L M Ruiz-Velasco

Hospital Universitario de La Princesa, Madrid, Madrid, Spain

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Publications (5)4.33 Total impact

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    ABSTRACT: Patients with cystic fibrosis are at great risk of infection by nontuberculous mycobacteria from the environment because of certain predisposing factors such as bronchiectasis, malnutrition, and diabetes. The aim of this study was to analyze the mycobacterial content of sputum smears and cultures from adult patients with cystic fibrosis attended at a specialized unit for adults from March 1997 through December 2001. Sputum samples were collected prospectively according to a protocol applied at each visit, and during most exacerbations staining and culture for mycobacteria were ordered in addition to the usual cultures for bacteria and fungi. A tuberculin test was performed at the end of the study. Twenty-eight patients (16 men) with cystic fibrosis were enrolled. The mean (SD) age was 25.3 (6.7) years. A total of 251 samples were cultured (range in number of samples per patient, 1-31). The mean period of follow up was 40.3 (22.1) months. The sputum smear was positive in 29 cases (4 patients); the culture was positive in 7 patients. More than 3 samples were positive in only 4 patients. Mycobacterium abscessus was isolated in 3 cases, Mycobacterium avium complex in 2 and Mycobacterium simiae in 1 and other an unidentified rapid growth Mycobacterium species. The Mantoux test was positive in 5 patients. Two of the 4 patients in whose samples mycobacteria were isolated repeatedly required treatment. The prevalence of nontuberculous mycobacterial infection is high in patients with cystic fibrosis. Staining and culture for mycobacteria should be carried out regularly and whenever exacerbation of pulmonary symptoms cannot be attributed to bacteria usually found in such patients. Patients with recurrent isolations of mycobacteria should be monitored closely.
    Archivos de Bronconeumología 11/2005; 41(10):560-5. · 1.37 Impact Factor
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    ABSTRACT: ObjectivePatients with cystic fibrosis are at great risk of infection by nontuberculous mycobacteria from the environment because of certain predisposing factors such as bronchiectasis, malnutrition, and diabetes. The aim of this study was to analyze the mycobacterial content of sputum smears and cultures from adult patients with cystic fibrosis attended at a specialized unit for adults from March 1997 through December 2001.Patients and methodsSputum samples were collected prospectively according to a protocol applied at each visit, and during most exacerbations staining and culture for mycobacteria were ordered in addition to the usual cultures for bacteria and fungi. A tuberculin test was performed at the end of the study.ResultsTwenty-eight patients (16 men) with cystic fibrosis were enrolled. The mean (SD) age was 25.3 (6.7) years. A total of 251 samples were cultured (range in number of samples per patient, 1-31). The mean period of follow up was 40.3 (22.1) months. The sputum smear was positive in 29 cases (4 patients); the culture was positive in 7 patients. More than 3 samples were positive in only 4 patients. Mycobacterium abscessus was isolated in 3 cases, Mycobacterium avium complex in 2 and Mycobacterium simiae in 1 and other an unidentified rapid growth Mycobacterium species. The Mantoux test was positive in 5 patients. Two of the 4 patients in whose samples mycobacteria were isolated repeatedly required treatment.ConclusionsThe prevalence of nontuberculous mycobacterial infection is high in patients with cystic fibrosis. Staining and culture for mycobacteria should be carried out regularly and whenever exacerbation of pulmonary symptoms cannot be attributed to bacteria usually found in such patients. Patients with recurrent isolations of mycobacteria should be monitored closely.ObjetivoLOS pacientes con fibrosis quística (FQ) presentan un mayor riesgo de infección por micobacterias ambientales en relación con ciertos factores predisponentes como bronquiectasias, desnutrición y diabetes. El objetivo del presente estudio es analizar los resultados de las baciloscopias y cultivos de micobacterias de esputos de pacientes con FQ de una unidad de adultos, entre marzo de 1997 y diciembre de 2001.Pacientes y métodosLas muestras de esputo se recogieron de forma prospectiva y protocolizada en cada visita y en la mayoría de las exacerbaciones, en las que, además de los cultivos bacterianos habituales y de hongos, se solicitaron tinción y cultivo para micobacterias. Se realizó la prueba de la tuberculina al final del estudio.ResultadosSe incluyó a 28 pacientes con FQ, 16 varones, con una edad media (± DE) de 25,3 ± 6,7 años. Se cultivaron un total de 251 muestras (rango por paciente de 1 a 31). El tiempo medio de seguimiento fue de 40,3 ± 22,1 meses. En 29 casos (4 pacientes) la baciloscopia fue positiva y se obtuvieron cultivos positivos en 7 pacientes, sólo en 4 en más de 3 muestras. Se aislaron: Mycobacterium abscessus en 3 casos, M. avium complex en 2 y M. simiae en uno y en otro una especie de crecimiento rápido no identificada. En 5 pacientes el Mantoux fue positivo. Dos de los 4 pacientes con aislamientos reiterados presentaron deterioro clínico y requirieron tratamiento.ConclusionesHay una alta prevalencia de micobacterias ambientales en pacientes con FQ. Habría que realizar tinción y cultivo para micobacterias de forma periódica y en caso de exacerbación pulmonar no atribuible a infección bacteriana habitual. Hay que vigilar estrechamente a los pacientes con aislamientos repetidos.
    Archivos de Bronconeumología ((English Edition)). 10/2005;
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    ABSTRACT: Objective Patients with cystic fibrosis are at great risk of infection by nontuberculous mycobacteria from the environment because of certain predisposing factors such as bronchiectasis, malnutrition, and diabetes. The aim of this study was to analyze the mycobacterial content of sputum smears and cultures from adult patients with cystic fibrosis attended at a specialized unit for adults from March 1997 through December 2001. Patients and methods Sputum samples were collected prospectively according to a protocol applied at each visit, and during most exacerbations staining and culture for mycobacteria were ordered in addition to the usual cultures for bacteria and fungi. A tuberculin test was performed at the end of the study. Results Twenty-eight patients (16 men) with cystic ibrosis were enrolled. The mean (SD) age was 25.3 (6.7) years. A total of 251 samples were cultured (range in number of samples per patient, 1-31). The mean period of follow up was 40.3 (22.1) months. The sputum smear was positive in 29 cases (4 patients); the culture was positive in 7 patients. More than 3 samples were positive in only 4 patients. Mycobacterium abscessus was isolated in 3 cases, Mycobacterium avium complex in 2 and Mycobacterium simiae in 1 and other an unidentified rapid growth Mycobacterium species. The Mantoux test was positive in 5 patients. Two of the 4 patients in whose samples mycobacteria were isolated repeatedly required treatment. Conclusions The prevalence of nontuberculous mycobacterial infection is high in patients with cystic fibrosis. Staining and culture for mycobacteria should be carried out regularly and whenever exacerbation of pulmonary symptoms cannot be attributed to bacteria usually found in such patients. Patients with recurrent isolations of mycobacteria should be monitored closely
    Archivos De Bronconeumologia - ARCH BRONCONEUMOL. 01/2005; 41(10):560-565.
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    ABSTRACT: Escherichia vulneris is an enterobacteriaceae seldom isolated in microbiology laboratories. We report two cases of infection with positive cultures for this microorganism. Microbiological cultures of wound secretion and peritoneal fluid on usual media. Identification by PASCO and API 20E systems. Antimicrobial sensitivity was performed by a commercial microdilution system. The first case is a postsurgical hand infection from which was isolated Escherichia vulneris with Enterococcus faecium, it shows the typical presentation of infection by this bacteria. The second case consists of a peritonitis after aggressive abdominal surgery. In the peritoneal fluid culture, Escherichia vulneris and Cedecea neteri were recovered. The isolation of Escherichia vulneris has not been reported previously in Spain. We contribute towards its better knowledge with two clinical reports: a typical infection and an exceptional isolation from peritoneal fluid.
    Enfermedades Infecciosas y Microbiología Clínica 01/1994; 11(10):559-61. · 1.48 Impact Factor
  • Enfermedades Infecciosas y Microbiología Clínica 22(6):369-70. · 1.48 Impact Factor