Publications (3)2.89 Total impact
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Article: A bovine whole-genome radiation hybrid panel and outline map.
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ABSTRACT: A 3000-rad radiation hybrid panel was constructed for cattle and used to build outline RH maps for all 29 autosomes and the X and Y chromosomes. These outline maps contain about 1200 markers, most of which are anonymous microsatellite loci. Comparisons between the RH chromosome maps, other published RH maps, and linkage maps allow regions of chromosomes that are poorly mapped or that have sparse marker coverage to be identified. In some cases, mapping ambiguities can be resolved. The RH maps presented here are the starting point for mapping additional loci, in particular genes and ESTs that will allow detailed comparative maps between cattle and other species to be constructed. Radiation hybrid cell panels allow high-density genetic maps to be constructed, with the advantage over linkage mapping that markers do not need to be polymorphic. A large quantity of DNA has been prepared from the cells forming the RH panel reported here and is publicly available for mapping large numbers of loci.Mammalian Genome 09/2002; 13(8):469-74. · 2.89 Impact Factor -
Article: Three bovine {alpha}2-fucosyltransferase genes encode enzymes that preferentially transfer fucose on Gal{beta}1-3GalNAc acceptor substrates
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ABSTRACT: To investigate the synthesis of α2-fucosylated epitopes in the bovine species, we have characterized cDNAs from various tissues. We found three distinct α2-fucosyltransferase genes, named bovine fut1 , fut2 , and sec1 which are homologous to human FUT1 , FUT2 , and Sec1 genes, respectively. Their open reading frames (ORF) encode polypeptides of 360 (bovine H), 344 (bovine Se), and 368 (bovine Sec1) amino acids, respectively. These enzymes transfer fucose in α1,2 linkage to ganglioside GM 1 and galacto- N -biose, but not to the phenyl-&bgr;- d -galactoside, type 1 or type 2 acceptors, suggesting that their substrate specificity is different and more restricted than the other cloned mammalian α2-fucosyltransferases. Southern blot analyses detected four related α2-fucosyltransferase sequences in the bovine genome while only three have been described in other species. The supernumerary entity seems to be related to the α2-fucosyltransferase activity which can also use type 1 and phenyl-&bgr;- d -galactoside substrate acceptors. It was exclusively found in bovine intestinal tract. Our results show that, at least in one mammalian species, four α2-fucosyltransferases are present, three adding a fucose on α1,2 linkage on type 3/4 acceptor (Gal&bgr;1–3GalNAc) and another able to transfer also fucose on phenyl-&bgr;- d -galactoside and type 1 (Gal&bgr;1–3GlcNAc) acceptors. The phylogenetic tree of the enzymes homologous to those encoded by the bovine fut1 , fut2 , and sec1 genes revealed two main families, one containing all the H-like proteins and the second containing all the Se-like and Sec1-like proteins. The Sec1-like family had a higher evolutionary rate than the Se-like family. -
Article: Organization of the Bovine {alpha}2-Fucosyltransferase Gene Cluster Suggests that the Sec1 Gene Might Have Been Shaped Through a Nonautonomous L1-Retrotransposition Event Within the Same Locus
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ABSTRACT: By referring to the split coding sequence of the highly conserved α6-fucosyltransferase gene family (assumed to be representative of the common α2 and α6 fucosyltransferase gene ancestor), we have hypothesized that the monoexonic coding sequences of the present α2-fucosyltransferase genes have been shaped in mammals by several events of retrotransposition and/or duplication. In order to test our hypothesis, we determined the structure of the three bovine α2-fucosyltransferase genes ( bfut1, bfut2, and sec1 ) and analyzed their characteristics compared with their human counterparts ( FUT1, FUT2, and Sec1 ). We show that in mammals, a complex nonautonomous L1-retrotransposition event occurred within the locus of the α2-fucosyltransferase ancestor gene itself. A consequence of this event was the processing in Catarrhini of a Sec1 pseudogene via several point mutations.
