Yu Zhang

State Key Laboratory of Medical Genetics of China, Ch’ang-sha-shih, Hunan, China

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Publications (452)1738.57 Total impact

  • Fuel Processing Technology 03/2016; 143:1-6. DOI:10.1016/j.fuproc.2015.11.004 · 3.35 Impact Factor
  • Yi Wang · Jing-Chun Feng · Xiao-Sen Li · Yu Zhang · Gang Li ·

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    ABSTRACT: HIV-1 infected patients frequently have osteolytic bone disease, which is caused by the dysregulation of the bone remodeling system that involves the interaction between osteoblasts and osteoclasts, but the relationship between osteolytic disease and HIV-1 infection remains unclear. In this study we tested whether HIV-1 infection of osteoclasts affects their differentiation. We prepared human osteoclasts from CD14+ monocytes and examined them for their susceptibility to HIV-1. Furthermore, we investigated the effect of HIV-1 infection on osteoclast differentiation. CD14-derived osteoclasts were shown to express CD4, CCR5, and CXCR4 each at the similar level to that shown with macrophages. R5-tropic HIV-1 and X4-tropic HIV-1 were found to infect CD14-derived osteoclasts and replicate in them. Furthermore, HIV-1 infection induced formation of larger osteoclastst, enhanced the expression of mRNAs for three osteoclast specific marker molecules (tartrate-resistant acid phosphatase, cathepsin K, and the calcitonin receptor), and up-regulated osteoclast bone resorption activity. Our results suggest that osteoclasts serve as a novel target for HIV-1 infection, which may enhance the osteoclast differentiation contributing to the development of osteolytic disease in HIV-1-infected patients.
    Retrovirology 12/2015; 12(1). DOI:10.1186/s12977-015-0139-7 · 4.19 Impact Factor
  • Jing-Chun Feng · Yi Wang · Xiao-Sen Li · Yu Zhang ·

    Energy & Fuels 11/2015; DOI:10.1021/acs.energyfuels.5b01970 · 2.79 Impact Factor
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    ABSTRACT: Cellular senescence impedes cancer progression by limiting uncontrolled cell proliferation. To identify new genetic events controlling senescence, we performed a small interfering RNA screenin human cancer cellsand identified a number of targets potentially involved in senescence of MDA-MB-231 human breast cancer cells. Importantly, we showed that knockdown of RAD21 resulted in the appearance of several senescent markers, including enhanced senescence-associated β-galactosidase activity and heterochromatin focus formation, as well as elevated p21 protein levels andRB1 pathway activation. Further biochemical analyses revealed that RAD21 knockdown led to the downregulation of c-Myc and its targets, including CDK4, a negative regulator of RB1, and blockedRB1 phosphorylation (pRB1) and the RB1-mediated transcriptional repression of E2F. Moreover, c-Myc downregulation was partially mediated by proteasome-dependent degradation within promyelocytic leukemia (PML) nuclear bodies, which were found to be highly abundant during RAD21 knockdown-induced senescence. Exogenous c-Myc reconstitution rescued cells from RAD21 silencing-induced senescence. Altogether, data arising from this study implicate a novel function of RAD21 in cellular senescence in MDA-MB-231 cells that is mainly dependent onRB1 pathway activation via c-Myc downregulation. This article is protected by copyright. All rights reserved.
    Journal of Cellular Biochemistry 11/2015; DOI:10.1002/jcb.25426 · 3.26 Impact Factor

  • Tetrahedron Letters 10/2015; DOI:10.1016/j.tetlet.2015.10.051 · 2.38 Impact Factor
  • Hong Zhang · Yu Zhang · Min Yang · Miaomiao Liu ·
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    ABSTRACT: While antibiotic pollution has attracted considerable attention due to its potential in promoting the dissemination of antibiotic resistance genes in the environment, the antibiotic activity of their related substances has been neglected, which may underestimate the environmental impacts of antibiotic wastewater discharge. In this study, a real-time quantitative approach was established to evaluate the residual antibacterial potency of antibiotics and related substances in antibiotic production wastewater (APW) by comparing the growth of a standard bacterial strain (Staphylococcus aureus) in tested water samples with a standard reference substance (e.g. oxytetracycline). Antibiotic equivalent quantity (EQ) was used to express antibacterial potency, which made it possible to assess the contribution of each compound to the antibiotic activity in APW. The real-time quantitative method showed better repeatability (Relative Standard Deviation, RSD 1.08%) compared with the conventional fixed growth time method (RSD 5.62-11.29%). And its quantification limits ranged from 0.20 to 24.00 μg L(-1), depending on the antibiotic. We applied the developed method to analyze the residual potency of water samples from four APW treatment systems, and confirmed a significant contribution from antibiotic transformation products to potent antibacterial activity. Specifically, neospiramycin, a major transformation product of spiramycin, was found to contribute 13.15-22.89% of residual potency in spiramycin production wastewater. In addition, some unknown related substances with antimicrobial activity were indicated in the effluent. This developed approach will be effective for the management of antibacterial potency discharge from antibiotic wastewater and other waste streams.
    Environmental Sciences: Processes and Impacts 09/2015; 17(11). DOI:10.1039/c5em00228a · 2.17 Impact Factor
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    ABSTRACT: Higher alcohols, longer chain alcohols, contain more than 3 carbon atoms, showed close energy advantages as gasoline, and were considered as the next generation substitution for chemical fuels. Higher alcohol biosynthesis by native microorganisms mainly needs gene expression of heterologous keto acid decarboxylase and alcohol dehydrogenases. In the present study, branched-chain α-keto acid decarboxylase gene from Lactococcus lactis subsp. lactis CICC 6246 (Kivd) and alcohol dehydrogenases gene from Zymomonas mobilis CICC 41465 (AdhB) were transformed into Escherichia coli for higher alcohol production. SDS-PAGE results showed these two proteins were expressed in the recombinant strains. The resulting strain was incubated in LB medium at 37 °C in Erlenmeyer flasks and much more 3-methyl-1-butanol (104 mg/L) than isobutanol (24 mg/L) was produced. However, in 5 g/L glucose-containing medium, the production of two alcohols was similar, 156 and 161 mg/L for C4 (isobutanol) and C5 (3-methyl-1-butanol) alcohol, respectively. Effects of fermentation factors including temperature, glucose content, and α-keto acid on alcohol production were also investigated. The increase of glucose content and the adding of α-keto acids facilitated the production of C4 and C5 alcohols. The enzyme activities of pure Kivd on α-ketoisovalerate and α-ketoisocaproate were 26.77 and 21.24 μmol min(-1) mg(-1), respectively. Due to its ability on decarboxylation of α-ketoisovalerate and α-ketoisocaproate, the recombinant E. coli strain showed potential application on isoamyl alcohol and isobutanol production.
    Journal of Industrial Microbiology 09/2015; 42(11). DOI:10.1007/s10295-015-1656-z · 2.44 Impact Factor

  • Letters in Drug Design &amp Discovery 09/2015; 12(999):1-1. DOI:10.2174/1570180812666150907203342 · 0.77 Impact Factor
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    ABSTRACT: MicroRNA-128 (miR-128) has been identified as a negative regulator of malignant phenotypes of prostate cancer (PCa) cells. The aim of this study was to evaluate the prognostic implications of both tissue and serum levels of miR-128 expression in PCa patients undergoing radical prostatectomy. A series of 128 cases with PCa were evaluated for both tissue and serum levels of miR-128 expression by quantitative reverse-transcription PCR. Compared with non-cancerous prostate tissues and normal sera, both tissue and serum levels of miR-128 expression were significantly decreased in PCa patients (both P<0.001). Importantly, there was a close correlation between tissue and serum levels of miR-128 expression in PCa patients (rs=0.808, P<0.001). Then, low miR-128 expression in both PCa tissues and patients' sera were dramatically associated with aggressive clinicopathological features, including advanced pathological stage (both P=0.001), positive lymph node metastasis (P=0.006 and 0.01, respectively), high preoperative PSA (both P=0.01) and positive angiolymphatic invasion (both P=0.02). Moreover, Kaplan-Meier survival analysis showed that low miR-128 expression in both PCa tissues and patients' sera were significantly associated with short biochemical recurrence (BCR)-free survival. Furthermore, multivariate analysis indicated that both tissue and serum levels of miR-128 expression were independent prognostic factors for BCR-free survival of PCa patients. Our data suggest that the decreased expression of miR-128 in both tissue and serum samples of PCa patients may be associated with tumor malignant progression and BCR-free survival. Particularly, serum miR-128 may be developed as a novel noninvasive biomarker for PCa diagnosis and prognosis.
    International journal of clinical and experimental pathology 09/2015; 8(7):8394-401. · 1.89 Impact Factor
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    ABSTRACT: Background: Upregulation of microRNA-221 (miR-221) has been reported to induce the malignant phenotype of human osteosarcoma, suggesting its potential as a therapeutic target for this malignancy. However, the role of miR-221 in diagnosis and prognosis of osteosarcoma has been well less elaborated. Our aim was to investigate the clinicopathological, diagnostic, and prognostic value of miR-221 in human osteosarcoma. Methods: Expression levels of miR-221 in tumor tissues and patients' sera obtained from 108 cases of primary osteosarcomas were detected by quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR). Results: Compared with normal controls, the expression levels of miR-221 in osteosarcoma tissues and patients' sera were both dramatically upregulated (both P=0.001). Then, receiver operating characteristics (ROC) analysis showed that serum miR-221 level could efficiently distinguish osteosarcoma patients from healthy controls (Area Under ROC Curve, AUC=0.844). Additionally, the serum level of miR-221 in osteosarcoma patients with positive distance metastasis (P=0.01) and advanced clinical stage (P=0.006) was significantly higher than those without distance metastasis and with early clinical stage. Moreover, we found that high serum miR-221 level was correlated with shorter recurrence-free survival (RFS) and overall survival (OS) than low level (both P=0.001). Multivariate survival analysis confirmed that serum miR-221 level was an independent prognostic factor influencing the survival of patients with osteosarcoma. Conclusion: These findings reveal that miR-221 may play a crucial role in the occurrence and the progression of human osteosarcoma. More importantly, miR-221 may function as a promising marker for screening individuals with osteosarcoma and for identifying individuals with poor prognostic potentials.
    Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 09/2015; 75. DOI:10.1016/j.biopha.2015.07.018 · 2.02 Impact Factor

  • Journal of Natural Gas Science and Engineering 09/2015; DOI:10.1016/j.jngse.2015.08.071 · 2.16 Impact Factor
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    ABSTRACT: Investigation of the optimal injection temperature for the hydrate dissociation plays a significant role in the gas hydrate exploitation in the practical field. In this work, the experiments of hydrate dissociation by depressurization in conjunction with thermal stimulation (DT) with the different injection temperatures are carried out in a Cubic Hydrate Simulator (CHS). Evaluation of the entropy production minimization (EPM), the energy ratio and the thermal efficiency are employed to investigate into the optimized injection temperature for hydrate dissociation. The thermal efficiency decreases with the increase of the injection temperature. The optimal injection temperatures for the hydrate dissociation from the points of the maximization of the energy ratio and the minimization of the entropy production, which are equivalent to maximizing the energy production and minimizing the energy consumption, respectively, are 38.8 °C and 37.9 °C. The results of evaluations from the two aspects are in a quite good agreement. Thus, the warm water injection of approximately 38-39 °C is suitable for hydrate dissociation with the DT method, and the hot water injection beyond 39 °C is uneconomical for hydrate dissociation.
    Applied Energy 09/2015; 154:995-1003. DOI:10.1016/j.apenergy.2015.05.106 · 5.61 Impact Factor
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    ABSTRACT: Ionic liquid-modified metal sulfides/graphene oxide nanocomposites are prepared via a facile electrostatic adsorption. Ionic liquid (IL) is firstly used as surface modifier and structure-directing agent of metal sulfide (MS) crystallization process, obtaining ionic liquid modified-MS (IL-MS) nanoparticles with positive charges on surface. IL-MS/GO is obtained by electrostatic adherence between positively charged IL-MS and negatively charged graphene oxide (GO). The as-prepared sample shows enhanced photocurrent and highly efficient photocatalytic activity under visible light irradiation, indicating IL-MS/GO nanocomposites greatly promoted the separation of photogenerated electron–hole pairs.
    Applied Surface Science 08/2015; 346. DOI:10.1016/j.apsusc.2015.03.213 · 2.71 Impact Factor
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    ABSTRACT: In this work, Raman spectroscopic analysis was applied to determine the structures and cage occupancies of the hydrates that formed from the system of flue gas (simulated by carbon dioxide–nitrogen–sulfur dioxide)–sulfur dioxide aqueous solution, and from the system of flue gas–sulfur dioxide containing tetra-n-butyl ammonium bromide (TBAB) aqueous solutions (sulfur dioxide mass concentration 0, 1.0, and 7.0 wt%). Comprehensive TBAB (solid, aqueous, and hydrate) Raman spectra were also obtained. The results reveal that when TBAB is used as promoter, both sulfur dioxide and carbon dioxide are encaged in the hydrate from systems of flue gas-TBAB solution with low sulfur dioxide concentration (0, 1.0 wt%), whereas in the hydrate from the system of flue gas-sulfur dioxide highly concentrated (7.0 wt%) TBAB solution, sulfur dioxide will be the sole gas guest encaged in the semi-clathrate hydrate. This suggests the sulfur dioxide concentration significantly influences the hydrate cage occupancies and separation selectivity of the hydrate-based technology. A two-stage hydrate-based flue gas purification process is proposed: one aims at desulfurization when sulfur dioxide concentrates to a relatively high level with the solutions recycling and in the other we can remove the sulfur dioxide and carbon dioxide simultaneously.
    Spectroscopy Letters 08/2015; 48(7). DOI:10.1080/00387010.2014.909854 · 0.85 Impact Factor
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    Jing-Chun Feng · Yi Wang · Xiao-Sen Li · Gang Li · Yu Zhang ·

    Energy 08/2015; DOI:10.1016/j.energy.2015.07.110 · 4.84 Impact Factor
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    ABSTRACT: Highly stable monodispersed nano Cu hydrosols were facilely prepared by an aqueous chemical reduction method through selecting copper hydroxide (Cu(OH)2) as the copper precursor, poly(acrylic acid) (PAA) and ethanol amine (EA) as the complexing agents, and hydrazine hydrate as the reducing agent. The size of the obtained Cu colloidal nanoparticles was controlled from 0.96 to 26.26 nm by adjusting the dosage of the copper precursor. Moreover, the highly stable nano Cu hydrosols could be easily concentrated and re-dispersed in water meanwhile maintaining good dispersibility. A model catalytic reaction of reducing p-nitrophenol with NaBH4 in the presence of nano Cu hydrosols with different sizes was performed to set up the relationship between the apparent kinetic rate constant (kapp) and the particle size of Cu catalysts. The experimental results indicate that the corresponding kapp showed an obvious size-dependency. Calculations revealed that kapp was directly proportional to the surface area of Cu catalyst nanoparticles, and also proportional to the reciprocal of the particle size based on the same mass of Cu catalysts. This relationship might be a universal principle for predicting and assessing the catalytic efficiency of Cu nanoparticles. The activation energy (Ea) of this catalytic reaction when using 0.96 nm Cu hydrosol as a catalyst was calculated to be 9.37 kJ mol(-1), which is considered an extremely low potential barrier. In addition, the synthesized nano Cu hydrosols showed size-dependent antibacterial activities against Pseudomonas aeruginosa (P. aeruginosa) and the minimal inhibitory concentration of the optimal sample was lower than 5.82 μg L(-1).
    Nanoscale 07/2015; 7(32). DOI:10.1039/c5nr03414k · 7.39 Impact Factor
  • Yunyun Liu · Jingliang Xu · Yu Zhang · Zhenhong Yuan · Jun Xie ·
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    ABSTRACT: Viscosity trends in alkali-pretreated sugarcane bagasse (SCB) slurries undergoing high solids fed-batch enzymatic hydrolysis were measured for a range of solids loading from 15% to 36%. Solids liquefaction times were related to system viscosity changes. The viscosity decreased quickly for low solids loading, and increased with increasing solids content. Fed-batch hydrolysis was initiated with 15% solids loading, and an additional 8%, 7% and 6% were successively added after the system viscosity decreased to stable values to achieve a final solids content of 36%. Two enzyme-adding modes with 8.5 FPU/g solid were investigated. The batch mode with all enzyme being added at the beginning of the reaction produced the highest yields, with approximately 231.7g/L total sugars and 134.9g/L glucose being obtained after 96h with nearly 60% of the final glucan conversion rate. This finding indicates that under the right conditions, the fed-batch strategy might be a plausible way to produce high sugars under high solids. Copyright © 2015. Published by Elsevier B.V.
    Journal of Biotechnology 07/2015; 211. DOI:10.1016/j.jbiotec.2015.06.422 · 2.87 Impact Factor
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    ABSTRACT: Sugars released from alkali-pretreated SCB (sugarcane bagasse) were used for biofuel (bioethanol and biogas) production based on a high-solids fed-batch SSF (simultaneous saccharification and fermentation) process with delayed inoculation (DSSF). A DSSF process with 24 h delayed inoculation increased the ethanol production rate by eliminating glucose inhibition in the early stages of fermentation, and shortened the duration of the process. Increasing solids loading from 18 to 36% (w/v) enhanced glucose concentration, while ethanol conversion efficiency was decreased. Gradual feeding of the hydrolyzed medium could improve the DSSF process. DSSF, with batch feeding mode, achieved as high as 68.047 g/L (74.13% of theoretical yield) ethanol concentration with 30% (w/v) solids loading at 96 h. After evaporation, the residual stillage obtained 306.974 mL/g volatile solids (VS) methane through anaerobic digestion. Sequential bioethanol and biogas production improved the yield of utilized biomass.
    Energy 07/2015; DOI:10.1016/j.energy.2015.06.066 · 4.84 Impact Factor

Publication Stats

9k Citations
1,738.57 Total Impact Points


  • 2015
    • State Key Laboratory of Medical Genetics of China
      Ch’ang-sha-shih, Hunan, China
    • The University of Hong Kong
      • Centre of Influenza Research
      Hong Kong, Hong Kong
  • 2013-2015
    • Harbin Medical University
      • Department of Pharmacology
      Charbin, Heilongjiang Sheng, China
    • Hangzhou University
      Hang-hsien, Zhejiang Sheng, China
    • Tsinghua University
      • Department of Chemical Engineering
      Peping, Beijing, China
  • 2012-2015
    • Huazhong University of Science and Technology
      • • School of Computer Science and Technology
      • • School of Public Health
      Wu-han-shih, Hubei, China
    • Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
      Hua-yang, Sichuan, China
  • 2011-2015
    • Fudan University
      • Department of Neurology
      Shanghai, Shanghai Shi, China
    • Jilin University
      • State Key Laboratory on Integrated Optoelectronics
      Yung-chi, Jilin Sheng, China
  • 2008-2015
    • East China University of Science and Technology
      • • School of Materials Science and Engineering
      • • School of Pharmacy
      Shanghai, Shanghai Shi, China
    • National Institute of Advanced Industrial Science and Technology
      Tsukuba, Ibaraki, Japan
    • Kunming Medical College
      Yün-nan, Yunnan, China
  • 2006-2015
    • Tianjin University
      • State Key Laboratory of Precision Measurement Technology and Instruments
      T’ien-ching-shih, Tianjin Shi, China
  • 2002-2015
    • Chinese Academy of Sciences
      • • State Key Laboratory of Environmental Aquatic Chemistry
      • • Laboratory of Energy Systems and Renewable Energy
      • • State Key Laboratory of Drug Research
      • • Research Center for Eco-Environmental Sciences
      • • Dalian Institute of Chemical Physics
      Peping, Beijing, China
  • 2014
    • Ministry Of Environmental Protection China
      Peping, Beijing, China
    • Sichuan University
      • State Key Laboratory of Polymer Material Engineering
      Hua-yang, Sichuan, China
    • North China University of Water Conservancy and Electric Power
      Cheng, Henan Sheng, China
    • Peking University
      • Department of Materials Science & Engineering
      Peping, Beijing, China
    • Peking University School of Stomatology
      Peping, Beijing, China
  • 2012-2014
    • University of Massachusetts Medical School
      • Gene Therapy Center
      Worcester, Massachusetts, United States
  • 2010-2014
    • Wuhan University
      • • State Key Laboratory of Virology
      • • College of Life Sciences
      Wu-han-shih, Hubei, China
    • West Anhui University
      Luchow, Anhui Sheng, China
  • 2007-2014
    • Kunming University of Science and Technology
      Yün-nan, Yunnan, China
    • Northeast Normal University
      • • Department of Chemistry
      • • The Institute of Genetics and Cytology
      Hsin-ching, Jilin Sheng, China
  • 2012-2013
    • Zhengzhou University
      • Department of Chemistry
      Cheng, Henan Sheng, China
  • 2008-2013
    • Hunan University
      • College of Environmental Science and Engineering
      Ch’ang-sha-shih, Hunan, China
  • 2007-2013
    • University of Science and Technology of China
      • • Department of Automation
      • • School of Life Sciences
      • • Department of Modern Physics
      Luchow, Anhui Sheng, China
  • 2011-2012
    • Zhejiang Academy of Agricultural Sciences
      Zhegang, Jiangxi Sheng, China
  • 2007-2011
    • National Institutes of Health
      • • Section on Developmental Genetics
      • • Laboratory of Cell and Developmental Biology
      Maryland, United States
  • 2007-2010
    • Tongji Hospital
      Wu-han-shih, Hubei, China
  • 2006-2010
    • Dalian Institute of Chemical Physics
      Lü-ta-shih, Liaoning, China
  • 2003-2010
    • Shanghai Institutes for Biological Sciences
      Shanghai, Shanghai Shi, China
  • 2008-2009
    • North China Electric Power University
      • School of Business Administration
      Peping, Beijing, China
  • 2006-2009
    • Technical Institute of Physics and Chemistry
      Peping, Beijing, China
  • 2007-2008
    • The National Institute of Diabetes and Digestive and Kidney Diseases
      Maryland, United States
    • Leiden University
      • Molecular and Development Genetics
      Leyden, South Holland, Netherlands