Publications (4)5.44 Total impact
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Article: Suppression of NK activity of spleen cells by chicken fetal antigen present on Marek's disease lymphoblastoid cell line cells
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ABSTRACT: Chicken fetal antigen (CFA) expressed on the cell surface of Marek's disease (MD) lymphoblastoid cell line (MDCC-MSBI) cells was purified by affinity chromatography using a monoclonal antibody (2H3). A 4-hr 51Cr release assay was performed using spleen cells as effector cells to investigate the role of the CFA in the immune response of chickens, especially in relation to natural killer (NK) activity. Spleen cells of specific-pathogen-free (SPF) chickens showed reduced NK activity in the presence of CFA in vitro, as did those cells treated with MSBI soluble antigen. NK activity of spleen cells from chickens treated with CFA was also suppressed when compared to the cells from untreated or ovalbumin-treated chickens. In addition, MSBI clo. 18 transplantable tumors grew progressively in some of the 14-day-old chickens treated with CFA, whereas the tumors regressed in age-matched chickens with or without treatment by ovalbumin.International Journal of Cancer 09/1987; 40(3):378 - 382. · 5.44 Impact Factor -
Article: BACTERIAL INFECTION WHICH PRODUCES HIGH MORTALITY IN CULTURED JAPANESE FLOUNDER (PARALICHTHYS OLIVACEUS) IN HOKKAIDO
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ABSTRACT: An infectious disease occurred in two groups of young Japanese flounder (Paralichthys olivaceus) reared in a commercial fish farm in Hokkaido in August 1985. These fish had been introduced in January 1985 from individual fish farms located in the southwestern part of Japan. More than 80% of the fish died within two weeks. Ulcerative lesions and loss of the skin were prominently observed, and the muscle was exposed. Hemorrhage and loss of fins, protrusion of the rectum, and swelling of the spleen were also observed. Edwardsiella tarda was isolated in pure culture from various tissues and organs. Japanese flounder inoculated with the isolate experimentally by infiltration or by an in traperitoneal route died one week after the inoculation. Swelling of abdomen and accumulation of ascitic fluid containing blood were significant. -
Article: A Two-Dimensional Electrophoretic Analysis of Serum Proteins of Rainbow Trout (Oncorhynchus mykiss) Treated with Inflammation Inducers
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ABSTRACT: Changes in serum proteins of rainbow trout (Oncorhynchus mykiss) treated with inflammation inducers or exposed to high temperature were analysed by using two-dimensional gel electrophoresis employing isoelectric focusing and sodium dodecyl sulfate polyacrylamide gel electrophoresis. Fishes were inoculated with lipopolysaccharide (LPS) or Freund's complete adjuvant (FCA) as inflammation inducer, or exposed to water temperature shift from 12℃ to 25℃. Increase of a protein of which apparent molecular weight of 43,000 and isoelectric point of 6.8 to 7.0 (43 kDa protein) was found in serum of fish inoculated with LPS or FCA. Contrary to this, few changes in serum protein compositions were observed in fish exposed to high temperature. The results suggest that the 43 kDa protein induced by LPS or FCA is a possible acute phase protein of rainbow trout. -
Article: Existence of cytotoxic acitivity against BLV-transformed cells in lymphocytes from normal cattle and sheep
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ABSTRACT: Peripheral blood lymphocytes (PBL) from normal cattle and sheep were tested for their cytotoxic activity against several target cells using a 20-hour 51Cr release assay. The following characteristics of the effector cells were observed; 1) PBL from animals showed cytotoxic activity against two sheep cell lines (FLK and SF-28) that were transformed with bovine leukemia virus. However, normal sheep and bovine cells and Molony leukemia virus-induced mouse lymphoma cell line (YAC-1) were not killed by these cells. 2) A time course study showed that the activity was first observed at 4 to 8 hours and reached a maximum at 20 to 30 hours after incubation. 3) Cytotoxic activity was observed in both adherent and nonadherent cell fractions when PBL were passed through a nylon-wool column. This indicated that the effector cells showed some degree of adherence. 4) Treatment of PBL with carrageenan did not change the cytotoxic activity against target cells, indicating that phagocytic capability is not perhaps necessary for cytotoxicity to take place. These results indicate that the effector cells participating in the cytotoxic reaction resembled natural killer cells or natural cytotoxic cells which are present in murine and human systems. However, analysis of the cell surface markers of the effector cells is yet to be done in future studies.Veterinary Immunology and Immunopathology.
