Publications (4)0 Total impact
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Shotaro Nishimura,
Masako Ootsu,
Ichiro Oshima,
Shoji Tabata,
Hisao Iwamoto,
正太郎 西村,
雅子 大津,
一郎 大島,
正志 田畑,
久雄 岩元,
ショウタロウ ニシムラ,
マサコ オオツ,
イチロウ オオシマ,
ショウジ タバタ, ヒサオ イワモト
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ABSTRACT: The three dimensional collagen architectures of rat, pig and chicken adenohypophyses were cmopared using a combination of cell maceration and scanning electron microscopy. The collagen network in the rat adenohypophysis was loose and the compartments of the cell clusters were obscure. The collagen fibils were more densely worked into the vascular walls than into the walls of the cell clusters. In contrast, the collagen construction in the pig adenohypophysis displayed obvious compartments in the walls of the clusters, and the thick bundles of collagen fibris were usually cylindrical in shape. In the chicken adenohypophysis, distinct cell clusters were separated by membrane-like collagen septa and the walls of these clusters were constructed of a very fine meshwork of collagen fiblis. These results indicate that the collagen architecture in the adenohypophysis differs among animal species, which may be due to differences in their gland sizes, cell distributions and cascular development.
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Nobuya Shiba,
Yoshi-Nori Nakamura,
Masatoshi Matsuzaki,
Shoji Tabata,
Shotaro Nishimura,
Eisaku Tsuneishi,
Hisao Iwamoto,
伸弥 柴,
好徳 中村,
正敏 松崎, [......],
正太郎 西村,
英作 常石,
久雄 岩元,
ノブヤ シバ,
ヨシノリ ナカムラ,
マサトシ マツザキ,
ショウジ タバタ,
ショウタロウ ニシムラ,
エイサク ツネイシ, ヒサオ イワモト
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ABSTRACT: Collagen content and architecture of the longissimus thoracis(LT) muscle were compared between the concentrate- and forage-fed male goats. During the experimentsl period of 9 to 26 wks old, the former gained 15.7kg in body weight by feedling on commercial concentrate food and Italian ryegrass hay, while the latter gained only 2.4kg by feedling on hay only. Dry matter, crude fat were contained at significantly higher percentages in the LT muscle of the concentrate-fed goats than the forage-fed goats, and conversaly, collagen content was significantly higher in the muscle of the latter. However, the collagen pyridinoline content did not differ between them. The myofibers in the concentrate-fed goats(54.0μm) were twice the size of those in the forage-fed goats(25.5μm). The higher content of crude fat(2.89%) in the LT muscle of the concentrate-fed goats was not enough for adipose tissues to develop within the perimysium as marbling. Immunohistochemical methods revealed typesⅠ and Ⅲ collagens over both perimysium and endomysium. Thin colagen bundles were loosely distributed in the goats muscles appeared as dense stacks of thick collagen fibers. From these results, it was indicate that the development of loose tissues of slender collagen fibers had an intimate relationship with the rapid growth rate of muscle rather than the development of adipose tissue.
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哲次 衛藤,
孝明 篠原,
登美子 文田,
建次 川畑,
邦仁 阪下,
正陽 大園,
久雄 岩元,
貴文 後藤,
Tetsuji Eto,
Taka-aki Shinohara, [......],
Hisao Iwamoto,
Takafumi Goto,
テツジ エトウ,
タカアキ シノハラ,
トミコ フミタ,
ケンジ カワバタ,
クニヒト サカシタ,
マサキヨ オオゾノ, ヒサオ イワモト,
タカフミ ゴトウ
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ABSTRACT: 黒毛和牛骨格筋における筋線維型構成と骨格筋内脂肪含量の関係について検討した．26－29カ月齢まで肥育された黒毛和牛より屠殺後，食肉生産上，重要な骨格筋である胸最長筋，大腿二頭筋，半膜様筋，半腱様筋，外側広筋，胸腹鋸筋および大腿直筋を摘出した．酵素組織化学的方法により筋線維をI型，IIA型およびIIB型筋線維に分類し（Brooke and Kaiser, 1970），筋線維型構成の算出を行った．またソックスレイ法によりそれぞれの骨格筋内の脂肪含量割合を測定した．黒毛和牛骨格筋の筋線維型構成に関して，骨格筋の種類により割合が異なった．すなわちI型筋線維を多く持つ胸腹鋸筋（61.7％），胸最長筋（45.8％），大腿二頭筋上部（52％），中程度持つ外側広筋（30.0％），大腿二頭筋下部（32.6％），およびI型筋線維がさらに少ない半腱様筋（24.6％），半膜様筋（26.4％），大腿直筋（23.3％）に分類された．一方，II型筋線維はI型筋線維と逆の傾向を示しその傾向はIIA型筋線維の変化というよりはIB型筋線維の変化として現れた．各骨格筋内の脂肪含量割合も骨格筋間で著しい差異が認められた．胸腹鋸筋は57.4％と最も大きく，以下胸最長筋27.9％，大腿二頭筋上部22.8％，大腿直筋15.7％，大腿二頭筋下部13.5％，外側広筋12.2％，半膜様筋10.6％，および半腱様筋10.4％の順で減少した．筋線維型構成割合と骨格筋内脂肪含量割合の相関関係を見ると，I型筋線維の割合とは正の，逆にIIA型およびIIB型筋線維の割合とは負の有意な相関関係が認められた．本研究より黒毛和牛去勢肥育牛において，骨格筋の種類により骨格筋内に蓄積される脂肪含量割合は著しく異なり，それは筋線維型構成と密接に関係していることが明らかになった． The interrelationship between myofiber type composition and intramuscular-fat content was investigated in muscles of Japanese Black cattle. The cattle were mainly fed with concentrate and slaughtered at 26-29 mo of age. After slaughter, M.longissimus thracis, M.biceps femoris, M.semimembranosus, M.semitendinosus, M. vastus lateralis, M.serratus ventralis and M.rectus femoris were removed from each carcass. Myofibers were enzyme-histochemically classified into type I, IIA and IIB myofibers according to the nomenclature of Brooke and Kaiser(1970). The percentage of intramuscular-fat content in each muscle was measured with Soxhlet method. In these muscles, the percentage of each type myofiber was related significantly to the percentage of intramuscular-fat content of each muscle at 26-29 mo of age. Correlation coefficient between the percentage of Type I myofiber and intramuscular-fat content was positive significant (p<0.005). Conversely, correlation coefficient between the percentages of type IIA, IIB myofiber and intramuscular-fat content was negative significant (p<0.05). These results indicated that the percentage of Type I myofiber was significantly related to the accumulation of intramuscular-fat in the muscles of Japanese Black cattle.
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雄二 塩塚,
哲次 衛藤,
久雄 岩元,
貴文 後藤,
Yuji Shiotsuka,
Tetsuji Eto,
Hisao Iwamoto,
Takafumi Goto,
ユウジ シオツカ,
テツジ エトウ, ヒサオ イワモト,
タカフミ ゴトウ
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ABSTRACT: 黒毛和種の種雄牛1頭と繁殖雌牛14頭を用いて，自然発情（自然発情区）後の自然交配と発情同期化処理に基づく発情誘起（発情誘起区）による自然交配における繁殖成績を調べ，人工授精における繁殖成績と比較検討した．発情同期化処理としてCIDR（イージーブリード）を利用したオブシンク（Ovsynch : Ovary Synchronization）プログラムを用いた．それぞれの延受胎率は自然交配の自然発情区93.3％，発情誘起区50.0％，人工授精の自然発情区42.4％，発情誘起区40.0％を示し，受胎した繁殖牛1頭当たりの授精回数はそれぞれ1.1回，2.0回，2.4回，および2.5回であった．自然交配の自然発情区では14頭中13頭が初回授精にて受胎した（92.9％）．また，自然交配の受胎間隔は人工授精のそれより有意に短縮され（83日短縮），345日であった（P＜0.01）．黒毛和種において種雄牛による自然交配により繁殖成績の向上，繁殖管理の省力化が図られ，同一種雄牛で同一月齢の子牛生産が可能になると推察された．黒毛和種種雄牛の飼養管理にかかる年間経費は，年間40頭の子牛を受胎させる繁殖経費とほぼ等しく，種雄牛による自然交配の導入は大規模繁殖農家にとって有効であると考えられる．今後は黒毛和種による自然交配で生産された子牛に対する市場評価を高めることが課題であろう． Reproductive efficiency of a Japanese Black bull to 14 cows was examined on the mating in natural estrus and introduced estrus caused by Ovsynch (Ovary Synchronization) program with CIDR (EAZI-BREED) for synchronization of estrus. The efficiency of the mating was compared with it of artificial insemination (AI) service. Conception rate on the mating to natural estrous cows was 93.3%, the number of fertilization per one conception was 1.1 times, and the scores on the mating to Ovsynch-treated cows were 50.0% and 2.0 services respectively. On AI service, conception rate in natural estrus was 42.4%, the number of fertilization per one conception was 2.4, and the scores in introduced estrus were 40.0% and 2.5 services respectively. 13 of 14 cows were conceived by the first mating in natural estrus. Moreover, conception interval on natural mating (345 days) was shorter than on AI services (428 days). From these result, it is suggested that natural mating by bull is quite useful to improvement of reproductive efficiency and labor-saving management of reproduction and make it possible to breed calves whose father is the same at the same season. On the reproductive cost, the introduction of bull is effective for farmers who raise more than 40 calves a year. To realize this natural mating in Japanese Black cows, the estimate of its produced calf would be essential on the market.
