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Publications (3)0 Total impact

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    ABSTRACT: Collagen content and architecture of the longissimus thoracis(LT) muscle were compared between the concentrate- and forage-fed male goats. During the experimentsl period of 9 to 26 wks old, the former gained 15.7kg in body weight by feedling on commercial concentrate food and Italian ryegrass hay, while the latter gained only 2.4kg by feedling on hay only. Dry matter, crude fat were contained at significantly higher percentages in the LT muscle of the concentrate-fed goats than the forage-fed goats, and conversaly, collagen content was significantly higher in the muscle of the latter. However, the collagen pyridinoline content did not differ between them. The myofibers in the concentrate-fed goats(54.0μm) were twice the size of those in the forage-fed goats(25.5μm). The higher content of crude fat(2.89%) in the LT muscle of the concentrate-fed goats was not enough for adipose tissues to develop within the perimysium as marbling. Immunohistochemical methods revealed typesⅠ and Ⅲ collagens over both perimysium and endomysium. Thin colagen bundles were loosely distributed in the goats muscles appeared as dense stacks of thick collagen fibers. From these results, it was indicate that the development of loose tissues of slender collagen fibers had an intimate relationship with the rapid growth rate of muscle rather than the development of adipose tissue.
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    ABSTRACT: グミ科, ヒッポファエ属の沙棘 (サジー, 学名:Hippophae rhamnoides L., 英語名:Seabuckthorn) は, 生理活性物質を豊富に含む植物である. ニーヴァンス (株) はサジーを用いて滋養強壮剤を開発中であるが, その搾り粕も栄養を豊富に含むため有効な活用法を模索している. 本研究では, 離乳直後の黒毛和牛15頭 (雄 9 頭, 雌 6 頭) を用いて, 配合飼料へのサジー搾り粕添加給与による離乳ストレスならびに成長に関する影響を血中成分により調査した. 対照区 ( 5 頭, 体重:81.1±4.6kg), サジー搾り粕 5 %添加区 ( 5 頭, 85.4±9.3kg) および同10%添加区 ( 5 頭, 80.2±6.4kg) を設定し, 離乳時から 6 カ月間の体重と血液性状の変化を調べた. 頚静脈から採血後, 血清中総蛋白質 (Biuret 法), アルブミン (BCG 法), 中性脂肪 (酵素法), γ-GTP (JSCC 準拠法), 免疫に関わる IgA, IgG および IgM (ネフェロメトリー法) 含量を測定した. 離乳時体重を基準とした場合のその後の成長に伴う体重比は, 対照区に比べて, 5 %と10%添加区で有意 (P <0.05) に低かった. 総蛋白質, アルブミンおよび中性脂肪含量は, 実験期間を通して変動したが一定の傾向はなかった. γ-GTP 含量は対照区と10%添加区に比べて, 5 %添加区では離乳時から 1 カ月目まで高値を示した (P <0.05). IgA, IgG および IgM 含量に一定の傾向はなかった. 以上より, 配合飼料へのサジー搾り粕添加 (10%以内) は, 牛の健康及び成長に害を及ぼすことはなかったが, 牛の血液性状ならびに体重増加にも特別の効果は及ぼさなかった. しかし, 育成期において増体の低下に影響することが示唆され, 配合飼料としての利用は直接添加することを避け, 加工や栄養素の抽出操作などを行う必要があるものと考えられた. Seabuckthorn (Hippophae rhamnoides L.) is the plant which contained many nutrients, for vitamin. In this study, the effect of seabuckthorn lees (SL) which added in concentrate feed on the growth and blood parameter, in particular factors in relation to stress in the weaning period was investigated using Japanese black cattle (n=15). Control group (n=5, body weight: 81.1±4.6kg) , 5% SL group (n=5, 85.4±9.3kg) and 10 % SL group (n=5, 80.2±6.4kg) were set up. The changes of body weight and blood parameter from wean to six months cattle were investigated. Body weight in 5 % and 10 % SL group were lower (P<0.05) than that of control group. There were differences of serum total protein, albumen and triglyceride among group. Contents of γ-GTP in 5% group was higher (P<0.05) those of control and 10 % group from weaning to one month. The contents of IgA, IgG and IgM were not changed. Therefore growth and blood parameter in cattle were not affected by the addition to SL (within 10 %). However, body weight in cattle decreased for SL addition. For use of SL, future study needs to investigate the processing and extracting of SL.
  • 佳典 仲村, ヨシノリ ナカムラ, Yoshinori Nakamura
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    ABSTRACT: 本研究ではMCP-1 値からみたエダラボンの効果について検討を行った。