Dan Zhang

Publications (3)4.99 Total impact

• Article: Induction of thymic stromal lymphopoietin expression in 16-HBE human bronchial epithelial cells by 25-hydroxyvitamin D3 and 1,25-dihydroxyvitamin D3.

  Dan Zhang, Chenghua Peng, Haijin Zhao, Yang Xia, Dandan Zhang, Hangming Dong, Jiafu Song, Liqin Zhou, Shaoxi Cai, Fei Zou
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  ABSTRACT: Vitamin D exerts profound effects on airway epithelial cells. Thymic stromal lymphopoietin (TSLP) derived from airway epithelial cells plays a role in the innate and antigen‑specific adaptive immune responses. However, the effect of vitamin D on TSLP expression in airway epithelial cells is unclear. In this study, 16-HBE human bronchial epithelial (HBE) cells were cultured with various concentrations of 25-hydroxyvitamin D3 (25 D3) and 1,25-dihydroxyvitamin D3 (1,25 D3). The expression of TSLP in the 16-HBE human bronchial epithelial cell line was analyzed by PCR and enzyme-linked immunosorbent assay (ELISA). We found that the 16-HBE cells converted inactive 25 D3 to active 1,25 D3 and that TSLP mRNA and protein expression levels were significantly increased, peaking at 2 or 12 h in the cells exposed to 500 nM 25 D3 and 50 nM 1,25 D3 respectively. Since vitamin D3 upregulated protein 1 (VDUP1) plays a multifunctional role in a variety of cellular responses, we hypothesized that VDUP1 is involved in the induction of TSLP production by 25 D3. The results showed that the mRNA and protein levels of VDUP1 were significantly upregulated by vitamin D. Furthermore, the silencing of VDUP1 by small interfering RNA (siRNA) significantly inhibited the 25 D3- and 1,25 D3-mediated induction of TSLP expression. To characterize the metabolic properties of vitamin D in airway epithelial biology, we used the chemical inhibitor of 1α-hydroxylase, itraconazole. The results revealed that itraconazole (10-6 M) reduced the 25 D3- but not the 1,25 D3-induced TSLP expression in 16-HBE cells. Based on these data, it can be concluded that vitamin D increases TSLP expression in 16-HBE cells through the VDUP1 pathway, which suggests a novel mechanism by which vitamin D alters immune function in the lungs.
  International Journal of Molecular Medicine 04/2013; · 1.98 Impact Factor
• Article: Erratum to: Increased heat shock protein 70 levels in induced sputum and plasma correlate with severity of asthma patients.

  Changchun Hou, Haijin Zhao, Wenjun Li, Zhenyu Liang, Dan Zhang, Laiyu Liu, Wancheng Tong, Shao-Xi Cai, Fei Zou
  Cell Stress and Chaperones 07/2011; · 3.01 Impact Factor
• Article: [Dimethylsulfoxide-induced expression of thymic stromal lymphopoietin in human bronchial epithelial cells].

  Dan Zhang, Shao-xi Cai, Hai-jin Zhao, Lai-yu Liu
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  ABSTRACT: To investigate the effect of dimethylsulfoxide on the expression of thymic stromal lymphopoietin (TSLP) in human bronchial epithelial cell (HBE). 16HBE cells were incubated in the presence of dimethylsulfoxide at different concentrations, and the cell proliferation changes were observed. The expressions of TSLP mRNA and protein in the cells were detected by real-time quantitative PCR and ELISA, respectively. Dimethylsulfoxide induced significantly increased TSLP mRNA expression in HBE cells (P<0.01) in a concentration-dependent manner. The level of TSLP protein in the supernatant was also increased after dimethylsulfoxide treatment, but high concentration of dimethylsulfoxide resulted in e inhibited cell proliferation. Dimethylsulfoxide may affect the immunomodulatory function of HBE cells.
  Nan fang yi ke da xue xue bao = Journal of Southern Medical University 05/2010; 30(5):963-5.