Eric Lader

Publications (2)11.05 Total impact

• Article: The role of upregulated miRNAs and the identification of novel mRNA targets in prostatospheres.

  Stephanie M Cabarcas, Suneetha Thomas, Xiaohu Zhang, James M Cherry, Thomas Sebastian, Subu Yerramilli, Eric Lader, William L Farrar, Elaine M Hurt
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  ABSTRACT: TICs are characterized by their ability to self-renew, differentiate and initiate tumor formation. miRNAs are small noncoding RNAs that bind to mRNAs resulting in regulation of gene expression and biological functions. The role of miRNAs and TICs in cancer progression led us to hypothesize that miRNAs may regulate genes involved in TIC maintenance. Using whole genome miRNA and mRNA expression profiling of TICs from primary prostate cancer cells, we identified a set of up-regulated miRNAs and a set of genes down-regulated in PSs. Inhibition of these miRNAs results in a decrease of prostatosphere formation and an increase in target gene expression. This study uses genome-wide miRNA profiling to analyze expression in TICs. We connect aberrant miRNA expression and deregulated gene expression in TICs. These findings can contribute to a better understanding of the molecular mechanisms governing TIC development/maintenance and the role that miRNAs have in the fundamental biology of TICs.
  Genomics 12/2011; 99(2):108-17. · 3.02 Impact Factor
• Source

  Available from: Tamara L Jones

  Article: Multiplexing siRNAs to compress RNAi-based screen size in human cells.

  Scott E Martin, Tamara L Jones, Cheryl L Thomas, Philip L Lorenzi, Dac A Nguyen, Timothy Runfola, Michele Gunsior, John N Weinstein, Paul K Goldsmith, Eric Lader, Konrad Huppi, Natasha J Caplen
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  ABSTRACT: Here we describe a novel strategy using multiplexes of synthetic small interfering RNAs (siRNAs) corresponding to multiple gene targets in order to compress RNA interference (RNAi) screen size. Before investigating the practical use of this strategy, we first characterized the gene-specific RNAi induced by a large subset (258 siRNAs, 129 genes) of the entire siRNA library used in this study ( approximately 800 siRNAs, approximately 400 genes). We next demonstrated that multiplexed siRNAs could silence at least six genes to the same degree as when the genes were targeted individually. The entire library was then used in a screen in which randomly multiplexed siRNAs were assayed for their affect on cell viability. Using this strategy, several gene targets that influenced the viability of a breast cancer cell line were identified. This study suggests that the screening of randomly multiplexed siRNAs may provide an important avenue towards the identification of candidate gene targets for downstream functional analyses and may also be useful for the rapid identification of positive controls for use in novel assay systems. This approach is likely to be especially applicable where assay costs or platform limitations are prohibitive.
  Nucleic Acids Research 01/2007; 35(8):e57. · 8.03 Impact Factor