V P Ladygina

Publications (14)5.69 Total impact

• Article: Mössbauer investigation of temperature transformations in bacterial ferrihydrite

  S. V. Stolyar, O. A. Bayukov, V. P. Ladygina, R. S. Iskhakov, L. A. Ishchenko, V. Yu. Yakovchuk, K. G. Dobretsov, A. I. Pozdnyakov, O. E. Piksina
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  ABSTRACT: Ferrihydrite nanoparticles formed as a result of the microorganism activity have been studied using Mössbauer spectroscopy, X-ray powder diffraction analysis, and X-ray fluorescence analysis. Three positions of trivalent iron with nonoverlapping ranges of quadrupole splittings have been revealed in bacterial ferrihydrite: QS{Fe3+(1)} = 0.49–0.83 mm/s, QS{Fe3+(2)} = 0.84–1.10 mm/s, and QS{Fe3+(3)} = 1.25–1.73 mm/s. It has been experimentally demonstrated that the Fe3+(3) positions are the centers of nucleation of the hematite phase in the course of heat treatment.
  Physics of the Solid State 05/2012; 53(1):100-104. · 0.71 Impact Factor
• Article: A Method for Introduction of Magnetic Nanoparticles into Tissues by Means of Magnetic Field Gradient: An Experimental Study

  K. G. Dobretsov, V. Yu. Afon’kin, A. K. Kirichenko, V. P. Ladygina, S. V. Stolyar, O. A. Bayukov, A. V. Sipkin
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  ABSTRACT: Targeted effects of magnetic nanoparticles were studied. Solution with iron-containing nanosubstance was applied to resected nasal bone and cartilage tissues. Magnetic field was generated by a Polus-101 device for low-frequency magnetotherapy, which provided permanent work of one inductor (10.14±19.56 mT). The results indicate that magnetic nanoparticles placed into magnetic field gradient penetrate into the thickness of the cartilage and bone tissues.
  Bulletin of Experimental Biology and Medicine 05/2012; 147(6):750-752. · 0.27 Impact Factor
• Article: Mössbauer study of bacterial ferrihydrite

  S. V. Stolyar, O. A. Bayukov, Yu. L. Gurevich, V. P. Ladygina, R. S. Iskhakov, P. P. Pustoshilov
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  ABSTRACT: Mössbauer measurements reveal four inequivalent Fe sites in ferrihydrite produced by Klebsiella oxytoca. The origin of these sites can be understood in terms of two nanosized structural regions in the bacterium and a certain ordering of bilayers and single layers of Fe-occupied octahedra.
  Inorganic Materials 04/2012; 43(6):638-641. · 0.41 Impact Factor
• Article: Mössbauer investigation of iron-producing bacteria Klebsiella oxytoca

  S. V. Stolyar, O. A. Bayukov, Yu. L. Gurevich, R. S. Ishkakov, V. P. Ladygina
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  ABSTRACT: The process of ferrihydrite formation in bacteria Klebsiella oxytoca has been investigated as a function of the bacteria cultivation time. The effect of temperature on the ferrihydrite transformations has been studied.
  Bulletin of the Russian Academy of Sciences Physics 04/2012; 71(9):1286-1290.
• Article: Iron-containing nanoparticles from microbial metabolism

  S. V. Stolyar, O. A. Bayukov, Yu. L. Gurevich, E. A. Denisova, R. S. Iskhakov, V. P. Ladygina, A. P. Puzyr’, P. P. Pustoshilov, M. A. Bitekhtina
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  ABSTRACT: We have studied the crystal structure and magnetic properties of Fe-containing nanoparticles formed as a result of microbial metabolism. Our experimental data, coupled with earlier results, suggest that the nanoparticles consist of ferrihydrite, Fe2O3 · nH2O, and are in a superparamagnetic state.
  Inorganic Materials 04/2012; 42(7):763-768. · 0.41 Impact Factor
• Article: Magnetic properties and application of biomineral particles produced by bacterial culture

  L. A. Ishchenko, S. V. Stolyar, V. P. Ladygina, Y. L. Raikher, M. Balasoiu, O. A. Bayukov, R. S. Iskhakov, E. V. Inzhevatkin
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  ABSTRACT: Ferrihydrite nanoparticles (2-5 nm in size) produced by bacteria culture in the course of biomineralization of iron salt solutions from a natural medium exhibit unique magnetic properties: they are characterized by both the antiferromagnetic order inherent in a bulk ferrihydrite and the spontaneous magnetic moment due to the decompensation of spins in sublattices of a nanoparticle. The magnetic susceptibility enhanced by the superantiferromagnetism effect and the magnetic moment independent of the magnetic field provide the possibility of magnetically controlling these natural objects. This has opened up the possibilities for their use in nanomedicine and bioengineering. The results obtained from measurements of the magnetic properties of the bacterial ferrihydrite in its two main crystalline modifications are reported. This has made it possible to determine numerical values of the magnetic parameters of real biomineral nanoparticles. (C) 2010 Published by Elsevier Ltd
  Physics procedia 01/2011; 9:279-282.
• Article: A method for introduction of magnetic nanoparticles into tissues by means of magnetic field gradient: an experimental study.

  K G Dobretsov, V Yu Afon'kin, A K Kirichenko, V P Ladygina, S V Stolyar, O A Bayukov, A V Sipkin
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  ABSTRACT: Targeted effects of magnetic nanoparticles were studied. Solution with iron-containing nanosubstance was applied to resected nasal bone and cartilage tissues. Magnetic field was generated by a Polus-101 device for low-frequency magnetotherapy, which provided permanent work of one inductor (10.14+/-19.56 mT). The results indicate that magnetic nanoparticles placed into magnetic field gradient penetrate into the thickness of the cartilage and bone tissues.
  Bulletin of Experimental Biology and Medicine 06/2009; 147(6):750-2. · 0.27 Impact Factor
• Article: [Experience with the use of magnetic nanoparticles in medicine and prospects for their application in otorhinolaringology].

  K G Dobretsov, V Iu Afon'kin, S V Stoliar, V P Ladygina, A V Sipkin, A S Lopatin
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  ABSTRACT: The literature review of the methods for application of magnetic nanoparticles in different fields of medicine is presented. The paper summarizes the 40 year-long experience of Russian and foreign scientists with the use of magnetic suspensions, liquids, and particles with magnetic carriers from 10 to 1000 nm in diameter. Results of recent studies are presented, prospects for the development and application of nanomedical techniques in otorhinolaringology are discussed.
  Vestnik otorinolaringologii 02/2009;
• Article: Bacterial leaching of manganese ores

  A. V. Belyi, P. P. Pustoshilov, Yu. L. Gurevich, G. G. Kadochnikova, V. P. Ladygina
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  ABSTRACT: Leaching of various types of ores, containing 12–30% manganese, by the thiobacterium Acidithiobacillus ferrooxidans was studied. Leaching of reduced ores (manganocalcite and manganiferous limestone) was mediated mainly by degradation of manganiferous minerals (by sulfuric acid produced in the course of bacterial oxidation of pyrite or sulfur). Bacterial treatment of the ores for 144 and 192 h allowed solubilization of 96–98% of manganese. Inoculation of bacteria into pulp with pyrite increased the rate of leaching of oxide ore (psilomelane) by 37%, and the degree of its extraction within 180 h increased from 80 to 97%.
  Applied Biochemistry and Microbiology 01/2006; 42(3):289-292. · 0.56 Impact Factor
• Article: Investigation of bacterioplankton in the loess-containing lake Khanka

  L. A. Shchur, A. D. Aponasenko, V. P. Ladygina, V. N. Lopatin, G. V. Makarskaya
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  ABSTRACT: Some characteristics of bacterioplankton—generation time, daily (P) and specific (P/B) bacterioplankton production, and bacterial metabolic coefficientK 2—in the loess-containing Lake Khanka were determined using five modifications of the bacterial-count procedure with the fluorescent dyes fluorescamin and erythrosin. Experiments showed that the organomineral complex (OMC) in this lake is broken down by chemoorganoheterotrophic bacteria. The increase in the loess content of the lake water intensified bacterial growth and the cycles of potassium, silicon, and other biogenic elements. The addition of starch to a loess suspension activated the breakdown of OMC due to the adsorption of starch on the OMC/water interface and stimulation of the metabolism of attached bacteria.
  Microbiology 06/2000; 69(4):466-470. · 3.06 Impact Factor
• Article: [Characteristics of bacterioplankton in the Loess-containing Lake Khanka].

  L A Shchur, A D Aponasenko, V P Ladygina, V N Lopatin, G V Makarskaia
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  ABSTRACT: Some characteristics of bacterioplankton--generation time, daily (P) and specific (P/B) bacterioplankton production, and bacterial metabolic coefficient K2--in the loess-containing Lake Khanka were determined using five modifications of the bacterial-count procedure with the fluorescent dyes fluorescamin and erythrosin. Experiments showed that the organomineral complex (OMC) in this lake is broken down by chemoorganoheterotrophic bacteria. The increase in the loess content of the lake water intensified bacterial growth and the cycles of potassium, silicon, and other biogenic elements. The addition of starch to a loess suspension activated the breakdown of OMC due to the adsorption of starch on the OMC/water interface and stimulation of the metabolism of attached bacteria.
  Mikrobiologiia 69(4):559-64.
• Source

  Available from: Victor Ivanovich Stepanov

  Article: Magnetic properties of biomineral particles produced by bacteria Klebsiella oxytoca

  Y. L. Raikher, V. I. Stepanov, S. V. Stolyar, V. P. Ladygina, D. A. Balaev, L. A. Ishchenko, M. Balasoiu
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  ABSTRACT: Ferrihydrite nanoparticles (2-5 nm in size) produced by bacteria Klebsiella oxytoca in the course of biomineralization of iron salt solutions from a natural medium exhibit unique magnetic properties: they are characterized by both the antiferromagnetic order inherent in a bulk ferrihydrite and the spontaneous magnetic moment due to the decompensation of spins in sublattices of a nanoparticle. The magnetic susceptibility enhanced by the superantiferromagnetism effect and the magnetic moment independent of the magnetic field provide the possibility of magnetically controlling these natural objects. This has opened up the possibilities for their use in nanomedicine and bioengineering. The results obtained from measurements of the magnetic properties of the ferrihydrite produced by Klebsiella oxytoca in its two main crystalline modifications are reported, and the data obtained are analyzed theoretically. This has made it possible to determine numerical values of the magnetic parameters of real biomineral nanoparticles.
  Physics of the Solid State. 52(2):298-305.
• Article: [Bacterial leaching of manganese ores].

  A V Belyĭ, P P Pustoshilov, Iu L Gurevich, G G Kadochnikova, V P Ladygina
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  ABSTRACT: Leaching of various types of ores, containing 12-30% manganese, by the thiobacterium Acidithiobacillus ferrooxidans was studied. Leaching of reduced ores (manganocalcite and manganiferous limestone) was mediated mainly by degradation of manganiferous minerals (by sulfuric acid produced in the course of bacterial oxidation of pyrite or sulfur). Bacterial treatment of the ores for 144 and 192 h allowed solubilization of 96-98% of manganese. Inoculation of bacteria into pulp with pyrite increased the rate of leaching of oxide ore (psilomelane) by 37%, and the degree of its extraction within 180 h increased from 80 to 97%.
  Prikladnaia biokhimiia i mikrobiologiia 42(3):327-31.
• Article: [Protist plankton from the Khanka lake].

  V P Ladygina, Iu L Gurevich
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  ABSTRACT: We present data on studies of protists in Lake Khanka in late August-early September. A total of 99 species of protists have been found, of them 14 phytoflagellates, 14 zooflagellates, five sarcodins, two heliozoans, 62 infusoria, and two suctorians. Small infusoria from the class of Kinetophragminophora, order Oligotrichida, as well as suborder Tetrahymenina were most numerous. The density of many protist species in Lake Khanka exceeds markedly that in several other high-productivity water reservoirs. Most of the protists belong to bacteriovores (bacteriophages). Many of the found species have high density. This is evidence of the production of bacterial plankton in the lake. In view of very high water turbidity in the lake, we assume that the total production of phytoplankton is not that high. In this connection, an abundance of protists (protist plankton) indicates is an indication for the existence of an additional source of organic matter in Lake Khanka.
  Izvestiia Akademii nauk. Seriia biologicheskaia / Rossiiskaia akademiia nauk