Jing Li

Wuhan General Hospital of Guangzhou Military Command, Wu-han-shih, Hubei, China

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Publications (6)11.85 Total impact

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    ABSTRACT: This study characterized the acaricidal activity of constituents of patchouli oil extracted from (Pogostemon cablin (Blanco) BENTH) against the house dust mite, Dermatophagoides farinae. A new compound, 2-(1,3-dihydroxy-but-2-enylidene)-6-methyl-3-oxo-heptanoic acid (DHEMH), was isolated from patchouli oil and characterized by (1)H-NMR, (13)C-NMR, LC-MS and elemental analysis (EA). This active component was identified as the hydrolysate of pogostone. Fifteen other constituents found in patchouli oil were also identified by GC-MS, including patchouli alcohol and pogostone. LD(50) studies carried out over 24 h using contact toxicity tests identified DHEMH as the most toxic compound to D. farinae (2.04 μg/cm(2)), followed by patchouli oil (6.11 μg/cm(2)), benzyl benzoate (BP) (9.31 μg/cm(2)) and dibutyl phthalate (DBP) (58.52 μg/cm(2)). In vapor phase toxicity tests, all of these compounds were more effective in closed than open containers, indicating that the most efficient mode of delivery for these compounds is the vapor phase. These results indicate that DHEMH and patchouli oil merit further study as potential agents for the control of D. farinae.
    Chemical & pharmaceutical bulletin 08/2012; 60(2):178-82. DOI:10.1002/chin.201232224 · 1.38 Impact Factor
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    ABSTRACT: In our effort to develop novel plant-derived acaricides, we examined the contact and fumigant toxicity of Asarum heterotropoides (Asarum sieboldii Miquel) essential oil constituents to the house dust mite Dermatophagoides farinae (Acari: Pyroglyphidae). Ten constituents, including methyl eugenol (relative amount 42.18 %), were identified by gas chromatography-mass spectroscopy (GC-MS) in the A. sieboldii Miq. essential oil. In contact toxicity tests, methyl eugenol (4.2 μg/cm(2), 24 h LD50) was most toxic to D. farinae, followed by benzyl benzoate (9.1 μg/cm(2)), A. sieboldii Miq. essential oil (37.7 μg/cm(2)), and dibutyl phthalate (DBP 57.9 μg/cm(2)). The potency of methyl eugenol and A. sieboldii Miq. essential oil was higher than benzyl benzoate and DBP, with mortalities of 100, 100, 94.6, and 13.2 %, respectively, after 2.5 h of exposure. In the vapor phase mortality bioassay, methyl eugenol and A. sieboldii Miq. essential oil resulted in 100 % mortality in closed containers after 24-h exposure, but only 4.7 and 7.9 %, respectively, in open containers, indicating that the toxicity in these tests was largely due to the vapor phase. Methyl eugenol and A. sieboldii Miq. essential oil merit further study as potential D. farinae control compounds.
    Parasitology Research 07/2012; 111(5):1895-9. DOI:10.1007/s00436-012-3032-5 · 2.33 Impact Factor
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    ABSTRACT: Early diagnosis and treatment is known to improve prognosis for nasopharyngeal carcinoma (NPC). The study determined the specific peptide profiles by comparing the serum differences between NPC patients and healthy controls, and provided the basis for the diagnostic model and identification of specific biomarkers of NPC. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) can be used to detect the molecular mass of peptides. Mass spectra of peptides were generated after extracting and purification of 40 NPC samples in the training set, 21 in the single center validation set and 99 in the multicenter validation set using weak cationic-exchanger magnetic beads. The spectra were analyzed statistically using FlexAnalysis™ and ClinProt™ bioinformatics software. The four most significant peaks were selected out to train a genetic algorithm model to diagnose NPC. The diagnostic sensitivity and specificity were 100% and 100% in the training set, 90.5% and 88.9% in the single center validation set, 91.9% and 83.3% in the multicenter validation set, and the false positive rate (FPR) and false negative rate (FNR) were obviously lower in the NPC group (FPR, 16.7%; FNR, 8.1%) than in the other cancer group (FPR, 39%; FNR, 61%), respectively. So, the diagnostic model including four peptides can be suitable for NPC but not for other cancers. FGA peptide fragments identified may serve as tumor-associated biomarkers for NPC.
    Journal of Cellular Biochemistry 07/2012; 113(7):2268-78. DOI:10.1002/jcb.24097 · 3.37 Impact Factor
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    ABSTRACT: This paper assessed the potential of traditional Chinese medicine (TCM) preparations for using as environmentally acceptable and alternative commercial acaricides. 22 kinds of TCM, which contained abundant essential oils and showed insecticidal effects, were collected. Samples extracted with petroleum ether, ethyl acetate and methanol were tested against house dust mites Dermatophagoides farinae and their toxicity assessed. The results showed that 3 TCM of Cinnamonum cassia, Eugenia caryophyllata and Pogostemon cablin have higher activity, and the parallel tests showed that the petroleum ether extract had higher activities (0.0046 mg/cm2, 0.005 mg/cm2 and 0.006 mg/cm2 respectively, 24 h, LD50) than the extracts of ethyl acetate and methanol. The acaricidal activity of the ethyl acetate extracts from C. cassia, P. cablin and Asarum sieboldii (0.00144 mg/cm2, 0.00347 mg/cm2 and 0.05521 mg/cm2 respectively, 24 h, LD50) were almost comparable to that of benzyl benzoate and dibutyl phthalate. However, the methanolic extracts of were less effective. CONCLUSIONS. This study shows the use of extracts with petroleum ether of C. cassia, P. cablin and E. caryophyllata as eco-friendly biodegradable agents for the control of the house dust mite.
    Parasitology 05/2010; 137(6):975-83. DOI:10.1017/S0031182009991879 · 2.35 Impact Factor
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    ABSTRACT: Volatile oil from the clove bud was extracted by petroleum ether using Soxhlet Extractor. The acaricidal activity was examined using direct contact and vapour phase toxicity bioassays. In a filter paper contact toxicity bio-assay, at 2.5 h after treatment, clove bud oil at a dose of 12.20 microg/cm2 killed all dust mites. As judged by 24-h LD50 values, potent fumigant action was observed with clove bud oil (12.20 microg/cm2), showing an adequate acaricidal activity against indoor Dermatophagoides farinae.
    Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases 12/2009; 27(6):492-3, 497.
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    ABSTRACT: Local nasal immunotherapy has been reported to be effective for airway allergic diseases. A biodegradable material, chitosan (CS), has been reported to be safe and effective in allergen delivery. In this study, we tested immunotherapeutic efficiency by intranasal administration of Der f entrapped in CS microparticles to sensitized mice. BALB/c mice were sensitized intraperitoneally with Der f extract absorbed to alum, followed by intranasal treatment with PBS, CS, Der f or Der f-CS nano-vaccine for 6 weeks. The mice were subsequently challenged intranasally with Der f extract for 1 week, and we analyzed their clinical symptoms, antibody expression levels, cytokine levels, T cell proliferation and regulatory T cell numbers. Mice treated with intranasal Der f-CS nano-vaccine prior to challenge displayed an alleviated spectrum of symptoms including airway hyper-reactivity, lung inflammation and mucus production and had fewer eosinophilic cells in bronchoalveolar lavage fluid (BALF). Interestingly, the cytokine levels in Der f-specific IgE were reduced, but IgA in serum and BALF was increased. We also observed that IL-4 was reduced and IFN-gamma and IL-10 were increased among splenocytes and in BALF, which inhibits Der f-specific T-cell proliferation in splenocytes and increases regulatory T cells in the spleen. However, the mice challenged without intranasal Der f or Der f-CS vaccine treatment developed allergic asthma. Our results illustrate that intranasal administration of Der f-CS nano-vaccine plays roles in immunologic protection in murine allergic asthma by inducing regulatory T cells and Th1-type reaction.
    International Archives of Allergy and Immunology 07/2009; 150(3):221-8. DOI:10.1159/000222674 · 2.43 Impact Factor

Publication Stats

26 Citations
11.85 Total Impact Points


  • 2012
    • Wuhan General Hospital of Guangzhou Military Command
      Wu-han-shih, Hubei, China
  • 2009–2012
    • Shenzhen University
      • • College of Life Sciences
      • • Allergy and Immunology Institute
      Bao'an, Guangdong, China