Azumi Yamagishi

Publications (2)3.76 Total impact

• Article: Polymorphisms in the human apolipoprotein E receptor 2 gene in Japanese sporadic Alzheimer's disease patients.

  Akemi Hosoda, Takashi Inoue, Chang C Mao, Young-Hee Jeong, Azumi Yamagishi, Mingjuan Ye, Tokuo Yamamoto, Dong-Ho Kim, Shigeru Saeki
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  ABSTRACT: Apolipoprotein E (apoE) polymorphism is associated with onset of Alzheimer's disease (AD). We found seven polymorphisms in apoE receptor 2 (ApoER2), an apoE-binding receptor, in Japanese sporadic AD patients, but no association of ApoER2 polymorphisms with AD. We consider that the functions of ApoER2 in the brain may be compensated for by those of other apoE-binding receptors such as VLDL receptor.
  Bioscience Biotechnology and Biochemistry 03/2010; 74(3):677-9. · 1.28 Impact Factor
• Article: The low-density lipoprotein receptor-related protein 10 is a negative regulator of the canonical Wnt/beta-catenin signaling pathway.

  Young-Hee Jeong, Manami Sekiya, Michiko Hirata, Mingjuan Ye, Azumi Yamagishi, Sang-Mi Lee, Man-Jong Kang, Akemi Hosoda, Tomoe Fukumura, Dong-Ho Kim, Shigeru Saeki
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  ABSTRACT: Wnt signaling pathways play fundamental roles in the differentiation, proliferation and functions of many cells as well as developmental, growth, and homeostatic processes in animals. Low-density lipoprotein receptor (LDLR)-related protein (LRP) 5 and LRP6 serve as coreceptors of Wnt proteins together with Frizzled receptors, triggering activation of canonical Wnt/beta-catenin signaling. Here, we found that LRP10, a new member of the LDLR gene family, inhibits the canonical Wnt/beta-catenin signaling pathway. The beta-catenin/T cell factor (TCF) transcriptional activity in HEK293 cells was activated by transfection with Wnt3a or LRP6, which was then inhibited by co-transfection with LRP10. Deletion of the extracellular domain of LRP10 negated its inhibitory effect. The inhibitory effect of LRP10 was consistently conserved in HEK293 cells even when GSK3beta phosphorylation was inhibited by incubation with lithium chloride and co-transfection with constitutively active S33Y-mutated beta-catenin. Nuclear beta-catenin accumulation was unaffected by LRP10. The present studies suggest that LRP10 may interfere with the formation of the beta-catenin/TCF complex and/or its binding to target DNA in the nucleus, and that the extracellular domain of LRP10 is critical for inhibition of the canonical Wnt/beta-catenin signaling pathway.
  Biochemical and Biophysical Research Communications 02/2010; 392(4):495-9. · 2.48 Impact Factor