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Gastroenterology 11/2011; 142(1):13, 188-9. · 11.68 Impact Factor
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The American Journal of the Medical Sciences 11/2011; 342(5):423. · 1.39 Impact Factor
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Gastroenterology 09/2011; 141(5):1565, 1951-2. · 11.68 Impact Factor
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ABSTRACT: Branched-chain amino acids (BCAAs) are one of the important biomarkers for monitoring liver disease such as hepatitis or hepatoma. In this communication, we present the determination of the concentrations of BCAA in ascites by CE light-emitted diode-induced fluorescence (LEDIF) using 1.5% m/v poly(ethylene oxide) (average M(v) : ~8 000 000 g/mol) that was prepared in 10 mM sodium tetraborate solution (pH 9.3). Naphthalene-2,3-dicarboxaldehyde was used to derivatize 15 amino acids (AAs) to form naphthalene-2,3-dicarboxaldehyde (NDA)-AA derivatives prior to CE analysis. The separation of 15 NDA-AA derivatives was accomplished within 15 min, with RSD values of <5.8% (within-day) and 7.4% (between-days) with respect to their migration times. The limits of detection for the tested BCAAs ranged from 10.6 to 10.9 nM. We determined the concentrations of three BCAAs--leucine, isoleucine and valine--in ascites by applying a standard addition method, with recovery percentages ranging from 93.9 to 111%. The results obtained from this CE-LEDIF method is in good agreement with those by a gold standard method using an AA analyzer. We have found that the concentrations of the three BCAAs in ascites obtained from patients suffering from liver diseases were lower than those from healthy individuals. Our approach is highly efficient, sensitive, and cost-effective, which holds great potential for the diagnosis of liver diseases.
Electrophoresis 03/2011; 32(9):1080-3. · 3.30 Impact Factor
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ABSTRACT: To investigate the antioxidative status of patients with alcoholic liver disease (ALD) in southeastern Taiwan.
Our study comprised 27 patients with ALD recruited from Taitung Mackay Memorial Hospital, located in southeastern Taiwan. Patients with ALD included 12 non-aborigines (12 men) and 15 aborigines (11 men and 4 women). According to the severity of ALD, patients with ALD included 10 with hepatitis (9 men and 1 woman) and 17 with cirrhosis (14 men and 3 women). Twenty-two age- and gender-matched healthy adults served as the control group in this study. Venous blood (10 mL) of each subject was drawn into EDTA-containing tubes after 8 h overnight fasting.
Compared to the control group, patients with ALD showed significantly lower erythrocytic catalase (11.1 ± 0.7 U/mg Hb vs 8.0 ± 0.7 U/mg Hb, P < 0.05) and superoxide dismutase (9.5 ± 1.6 U/mg Hb vs 3.0 ± 0.2 U/mg Hb, P < 0.05) activities. Furthermore, the erythrocytic reduced glutathione/oxidized glutathione ratio was significantly lower in ALD patients than that in the control group (38.1 ± 5.4 vs 15.7 ± 1.9, P < 0.05). The results revealed that patients with ALD experienced more oxidative stress than those in the control group. The non-aboriginal, but not the aboriginal, ALD group had higher erythrocytic glutathione peroxidase (GPX) activity than that in the control group (46.1 ± 7.8 U/g Hb vs 27.9 ± 2.2 U/g Hb, P < 0.05). Hepatitis, but not cirrhosis, ALD patients had higher erythrocytic GPX activity than that in the control group (44.3 ± 8.6 U/g Hb vs 27.9 ± 2.2 U/g Hb, P < 0.05).
Our results indicate that both ethnicity and the severity of ALD may cause different erythrocytic antioxidative enzyme activities especially GPX activity.
World Journal of Gastroenterology 02/2011; 17(8):1063-70. · 2.47 Impact Factor
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Gastrointestinal endoscopy 01/2011; 73(1):159; discussion 159-60. · 6.71 Impact Factor
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Gut 01/2011; 60(1):16, 48. · 10.11 Impact Factor
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The American Journal of the Medical Sciences 11/2010; 340(5):413. · 1.39 Impact Factor
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The American journal of emergency medicine 11/2010; 28(9):1061.e1-3. · 1.54 Impact Factor
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The American Journal of the Medical Sciences 10/2010; 340(4):319. · 1.39 Impact Factor
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ABSTRACT: No studies focus on the population with perforated peptic ulcer in southeastern Taiwan. The present study aimed to assess the differences between the different races and the risk factors related to mortality and morbidity in postoperative patients in southeastern Taiwan.
The medical records of 237 patients were reviewed retrospectively. The following factors were analyzed: patient profiles, coexisting illnesses, diagnostic method, fever, preoperative shock, clinical data at emergency room, delay operation, site of perforation, operative method, positive ascites culture, species of microbes in ascites culture, postoperative complications, death and the length of hospital stay.
Aborigines were significantly different from non-aborigines in the ratio of female cases and in the habits of alcohol drinking and betel nut chewing. There were also four significantly different variables between them: fever, hemoglobin value, site of perforation and operative method. Total postoperative complication rate was 41.3% and 39 patients (16.6%) died. In multivariate analysis, age > or = 65 years, lipase > upper normal limit and preoperative shock were independent predictors of mortality. Significant risk factors associated with morbidity were NSAIDs use, creatinine > 1.5 mg/dL and preoperative shock.
Aborigines were different from non-aborigines in several categories. In southeastern Taiwan, NSAIDs use, creatinine > 1.5 mg/dL and preoperative shock were independent risk factors of morbidity, and age > or = 65 years, lipase > upper normal limit and preoperative shock were independent risk factors of mortality in postoperative perforated peptic ulcer. Lipase > upper normal limit is needed for further research on the influence on mortality.
Journal of Gastroenterology and Hepatology 09/2010; 25(9):1530-6. · 2.87 Impact Factor
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Gastrointestinal endoscopy 08/2010; 72(2):432-3; discussion 433. · 6.71 Impact Factor
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Clinical gastroenterology and hepatology: the official clinical practice journal of the American Gastroenterological Association 06/2010; 8(6):e61-2. · 5.64 Impact Factor
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The American Journal of the Medical Sciences 06/2010; 339(6):573. · 1.39 Impact Factor
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The American Journal of the Medical Sciences 05/2010; 339(5):485. · 1.39 Impact Factor
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Gastrointestinal endoscopy 04/2010; 71(4):844-5; discussion 845. · 6.71 Impact Factor
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Gut 03/2010; 59(3):406, 414. · 10.11 Impact Factor
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The American Journal of the Medical Sciences 03/2010; 339(6):574. · 1.39 Impact Factor
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The American Journal of the Medical Sciences 02/2010; 339(4):371. · 1.39 Impact Factor
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Gastroenterology 02/2010; 138(4):e5-6. · 11.68 Impact Factor