[Show abstract][Hide abstract] ABSTRACT: The purpose of the present study was to develop a new method of chemoembolization to improve the therapeutic effectiveness and safety profile of cancer treatment. A chemoembolization approach was designed for human solid tumors using resorbable calcium-phosphate ceramic microspheres loaded with an agent anti-angiogenic to tumor vasculature in vivo. The human uterine sarcoma cell line FU-MMT-3 was used in this study because this tumor is aggressive and also exhibits a poor response to radiotherapy or any chemotherapy currently used. The calcium-phosphate ceramic microspheres loaded with TNP-470, an anti-angiogenic agent, were injected into FU-MMT-3 xenografts in nude mice three times per week for 8 weeks. The treatment using TNP-470-loaded microspheres suppressed tumor growth, compared to treatment with TNP-470 alone, microspheres alone, and the control. The mean tumor weight after treatment using TNP-470-loaded microspheres was significantly lower than that after treatment with microspheres alone. These ceramic microspheres were remarkably embolized in tumor microvessels as well as in the feeding arteries and a significant reduction of intratumoral vascularity was also demonstrated following treatment with TNP-470-loaded microspheres. Severe loss of body weight was not observed in any mice treated with the TNP-470-loaded microspheres, compared to treatment with TNP-470 alone. These results suggest that targeting tumor vasculature in human uterine sarcoma using calcium-phosphate microspheres might be more effective and safer than the treatment that employs anti-angiogenic agent alone. This new chemoembolization method incorporating an anti-angiogenic agent may contribute to the effective treatment of locally advanced or recurrent solid tumors.
(Cancer Sci 2010; 101: 984–990)
Cancer Science 04/2010; 101(4):984-90. DOI:10.1111/j.1349-7006.2009.01479.x · 3.53 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: We performed to synthesize the calcium-phosphate microsphere with well-controlled particle size by changing the frequency of the ultrasonic transducer. The crystalline phases of the resulting powders were composed of β-tricalcium phosphate (β-TCP) and hydroxyapatite (HAp); the Ca/P molar ratios were about 1.50. The SEM observation indicated that the resulting powders were composed of microspheres with a diameter of ~1 to ~2.5 μm. The particle sizes increased with decreasing ultrasonic frequency, and the distributions were quite narrow. The above results show that the particle size can be easily controlled by changing the frequency of ultrasonic transducer during spray-pyrolysis.
Phosphorus Research Bulletin 01/2005; 19:1-6. DOI:10.3363/prb1992.19.0_1