Zhi-Ming Li

Sun Yat-Sen University, Guangzhou, Guangdong Sheng, China

Are you Zhi-Ming Li?

Claim your profile

Publications (3)1.02 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: 1. Cell transplantation has promise as a therapeutic option for restoring impaired heart function after acute myocardial infarction (AMI). However, the optimal cell type to use remains controversial. We investigated the therapeutic efficacy and feasibility of intramyocardial transplantation of human umbilical cord blood-derived endothelial progenitor cells (hUCB-EPC) in rats with AMI. 2. The Wistar rats myocardial infarction model was established by ligating the left anterior descending artery. The labelled hUCB-EPC were transplanted through intramyocardial injection. Left ventricular function was assessed using a pressure-volume catheter and echocardiogram. Anti-VIII immunohistochemistry staining was used to reflect the degree of angiogenesis in peri-infarcted areas by calculating the average capillary density. The fibrosis degree of infarcted myocardium was analysed by Masson staining and the collagen volume fraction was calculated. 3. The labelled donor endothelial progenitor cells were detected in the new microvessels in host myocardium by double-positive staining with CM-Dil and FITC-UEA-l. An increase in left ventricular ejection fraction, left ventricular fractional shortening, left ventricular end-systolic pressure, first derivative of left ventricular pressure (+dP/dtmax and -dP/dtmax), as well as a decrease in the left ventricular end-diastolic pressure in rats with cell therapy indicated a significant improvement in global heart function. The cell therapy group had increased microvessel formation and a decreased degree of myocardial fibrosis compared to the control group. Moreover, the degree of myocardial fibrosis was less than that of the control group. 4. The improved global heart function and decreased cardiac fibrosis in rats with AMI implies the potential benefit of hUCB-EPC transplantation.
    Clinical and Experimental Pharmacology and Physiology 05/2010; 37(5-6):551-6. DOI:10.1111/j.1440-1681.2010.05347.x
  • [Show abstract] [Hide abstract]
    ABSTRACT: Summary 1.Cell transplantation has promise as a therapeutic option for restore impaired heart function after acute myocardial infarction. However the use of optimal cell type remains controversial. We investigated the therapeutic efficacy and feasibility of intramyocardial transplantation of human umbilical cord blood-derived endothelial progenitor cells (hUCB-EPCs) in rats with acute myocardial infarction. 2.The Wistar rats myocardial infarction model was established by ligating the left anterior descending artery. The labeled hUCB-EPCs were transplanted through intramyocardial injection. Left ventricular function was assessed by a pressure-volume catheter and echocardiogram. The anti-VIII immunohistochemistry staining was used to reflect the degree of angiogenesis in peri-infarcted area by calculating the average capillary density. The fibrosis degree of infarcted myocardium was analyzed by Masson staining and collegen volume fraction was calculated. 3.The labeled donor EPCs were detected in the new microvessels in host myocardium by double-positive for CM-Dil and FITC-UEA-l staining. The increase in left ventricular ejection fraction, left ventricular fractional shortening, left ventricular end-systolic pressure, first derivative of LVP (+dP/dtmax and -dP/dtmax), as well as decrease in the left ventricular end- diastolic pressure in rats with cell therapy indicated a significant improvement of global heart function. Cell therapy group was associated with increased microvessel formation and decreased degree of myocardial fibrosis compared to the control group. Moreover, the degree of myocardial fibrosis was less than that of the control group. 4.The improved global heart function and decreased cardiac fibrosis in rats with acute myocardial infarction may imply the potential benefit of hUCB-EPCs transplantation.
    Clinical and Experimental Pharmacology and Physiology 12/2009; DOI:10.1111/j.1440-1681.2009.05347.x
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Cell-based vascular therapies of endothelial progenitor cells (EPCs) mediated neovascularization is still a novel but promising approach for the treatment of ischemic disease. The present study was designed to investigate the therapeutic potentials of human umbilical cord blood-derived EPCs (hUCB-EPCs) in rat with acute myocardial infarction. Human umbilical cord blood (hUCB) mononuclear cells were isolated using density gradient centrifugation from the fresh human umbilical cord in healthy delivery woman, and cultured in M199 medium for 7 days. The EPCs were identified by double-positive staining with 1, 1'-dioctadecyl-3, 3, 3', 3'-tetramethylindocarbocyanine percholorate-labeled acetylated low-density lipoprotein (Dil-Ac-LDL) and fluorescein isothiocyanate-conjugated Ulex europaeus lectin (FITC-UEA-l). The rat acute myocardial infarction model was established by the ligation of the left anterior descending artery. The hUCB-EPCs were intramyocardially injected into the peri-infarct area. Four weeks later, left ventricular function was assessed by a pressure-volume catheter. The average capillary density (CAD) was evaluated by anti-VIII immunohistochemistry staining to reflect the development of neovascularization at the peri-infarct area. The graft cells were identified by double immunofluorescence staining with human nuclear antigen (HNA) and CD31 antibody, representing human origin of EPCs and vascular endothelium, respectively. Expressions of cytokines, proliferating cell nuclear angigen (PCNA), platelet endothelial cell adhesion molecule (PECAM) and vascular endothelial growth factor (VEGF) were detected to investigate the underlying mechanisms of cell differentiation and revascularization. The donor EPCs were detectable and integrated into the host myocardium as confirmed by double-positive immunofluorescence staining with HNA and CD31. And the anti-VIII staining demonstrated a higher degree of microvessel formation in EPCs transplanted rats, associated with a significant improvement of global heart function in terms of the increase of left ventricular end-systolic pressure (LVESP), +dp/dtmax and -dp/dtmax as well as the decrease of LVEDP in rats with EPCs therapy comparing to the control rats (P < 0.05). Moreover, the expression of the rat PCNA mRNA and PECAM were both enhanced in the EPCs group compared with that of the control group. The human umbilical cord blood-derived EPCs could incorporate into new-born capillaries in rat myocardium, induce revascularization and improve the proliferation activity in the peri-infarct area, resulting in the improvement of global heart function. This may indicate a promising stem cell resource in cell-based therapy for ischaemic diseases.
    Chinese medical journal 04/2009; 122(5):548-55. · 1.02 Impact Factor