Farzana Rashid

Publications (2)5.22 Total impact

• Source

  Available from: PubMed Central

  Article: Prevalence of HCV among the high risk groups in Khyber Pakhtunkhwa.

  Ijaz Ali, Lubna Siddique, Latif U Rehman, Najib U Khan, Aqib Iqbal, Iqbal Munir, Farzana Rashid, Sana U Khan, Safira Attache, Zahoor A Swati, Mehwish S Aslam
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  ABSTRACT: Hepatitis C is an infectious disease, caused by blood borne pathogen; the Hepatitis C Virus. In this study we analyzed blood samples collected from various risk groups for the prevalence of anti-HCV and active HCV infection with the help of Immunochromtographic tests and nested PCR. The prevalence of active HCV infection among the high risk groups was 15.57% (26/167). The prevalence of HCV in individual risk groups was 15%, 28%, 8%, 14.28% and 14.28% in the case of thalassemics, dialysis, major surgery group, dental surgery group and injection drug users respectively. Our analysis reveals the fact that health care facilities in the Khyber Pakhtunkhwa province of Pakistan are contributing a great deal towards the spread of HCV infection.
  Virology Journal 01/2011; 8:296. · 2.34 Impact Factor
• Article: Cis-regulatory elements affecting the Nanos gene promoter in the germline stem cells.

  Ijaz Ali, Muti ur Rehman, Farzana Rashid, Sanaullah Khan, Aqib Iqbal, Xia Laixin, Naeem ud din Ahmed, A Zahoor Swati
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  ABSTRACT: Drosophila Nanos gene plays an important role in stem cell maintenance and body patterning. With the purpose of understanding the cis-regulatory machinery involved in the transcription of the nanos gene in the germline stem cells, we examined its promoter fragment from +97 to -708 relative to the transcription start site and identified enhancer elements located between position -108 and +97. Experiments with transgenic flies revealed that the minimal promoter (from -108 to +20) is sufficient in the germline stem cells for the GFP expression in transgenic Drosophila. Moreover, the flag-tagged nanos protein blotting experiments revealed that a short promoter fragment plus some sequences of the nos 5'UTR spanning -108 to +97 could efficiently drive the expression of the flag-tagged [Nos-mRNA-nos3'UTR] transgene in transgenic flies indicating that the cis-regulatory elements located between positions -108 and +97 of the nanos promoter are sufficient to fully transcribe the nanos mRNA. Deletion of the identified cis-acting sequences from the promoter rendered it non-functional as it could no longer transcribe the nanos mRNA in transgenic flies thus revealing the importance of these sequences for the transcription of the nanos gene.
  Journal of biotechnology 12/2009; 145(4):323-9. · 2.88 Impact Factor