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ABSTRACT: To investigate the changes in the levels of DNA methylation in the testis during development after neonatal transient exposure to DNA methyltransferase (DNMT) inhibitors, we orally administered Sprague-Dawley (SD) rats with 5-aza-2'-deoxycytidine (5-aza-CdR; 0.025 or 0.25 mg kg(-1)) or cadmium (Cd; 1, 2 or 4 mg kg(-1)) daily from days 3-7 postpartum (pp). Sperm numbers decreased at day 70 pp in all exposure groups. We then used a PCR-based assay, combined bisulfite restriction analysis (COBRA) and pyrosequencing to determine the degrees of DNA methylation. Both 5-aza-CdR and Cd reduced DNMT activity in vivo after 5 days' exposure at day 8 pp but not at day 70 pp. In contrast, the DNA methylation level of LINE-1 was not changed in the testis, either at day 8 pp or at day 70 pp. We observed increased apoptosis and an increase in the p53 mRNA level, accompanied by a decreased DNA methylation level in the p53 gene promoter region, at day 8 pp in testis for the 5-aza-CdR-exposed groups but not in the Cd-exposed group. The Cd-exposed group exhibited a degradation of seminiferous tubules and inhibition of a stepwise change in methylation in the coding region of c-fos in testis at day 70 pp. Because we observed toxic phenotype development accompanied by aberrant DNA methylation, DNA methylation may play a role in chemical induced testis damage, with different DNMT inhibitors affecting DNA methylation levels in gene- or stage-specific manner.
Journal of Applied Toxicology 03/2011; 31(5):484-95. · 2.48 Impact Factor
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ABSTRACT: To evaluate the surgical management for the severe stenosis of peripheral esophageal access.
Twenty-three patients who underwent severe stenosis of peripheral esophageal access from 2000 to 2009, were retrospectively reviewed. The surgical plan was selected by considering the stage of stenosis, dilatation, reconstruction of hypopharynx, oesophagus or larynx.
Twenty-two patients resumed a normal oral diet and 21 cases succeeded in decannulation, no stenosis recurred after a 1-9 years follow-up.
The severe stenosis of peripheral esophageal access can get good results when the appropriate surgical plan were choice.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 11/2010; 24(21):972-4.
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ABSTRACT: The aim of the present study is to determine the long-term testicular effects of neonatal exposure to PCB153.
Sprague-Dawley (SD) rats were treated by oral gavage with PCB153 in corn oil at doses of 0, 0.025, and 2.5 mg/kg per day from postnatal day 3 (PND 3) to PND7. The rats were sacrificed on PND 8 and PND 77. TUNEL in situ detection for testis apoptosis, immunohistochemical staining of thyroid gland for thyroxine (T4), semi-quantitative RT-PCR for mRNA expression, and radioimmunoassay (RIA) for serum hormone levels were performed.
Neonatal treatment with PCB153 at both doses had no obvious effects on body weight, testis weight, testis histology, and germ cell apoptosis, but decreased T4 staining in thyroid gland was observed on PND 8. On PND 77, neonatal treatment with 2.5 mg/kg per day of PCB153 significantly reduced daily sperm product (DSP). Serum levels of thyroxine (T4) and free thyroxine (FT4) decreased, but there were no differences in thyroid-stimulating hormone (TSH) level between the control and exposed groups. Gap junction connexin43 (CX43) and cyclin-dependent kinase inhibitor (CDKI) P27kip1 mRNA expression, which was associated with Sertoli cell differentiation, was significantly reduced after PCB153 treatment on PND 8 but not on PND 77. Androgen-binding protein (ABP) and androgen receptor (AR) mRNA expression, which indicates Sertoli cell maturation, was suppressed on PND 77 after neonatal PCB153 exposure.
The findings in this study suggest that neonatal exposure to PCB153 induces persistent T4 reduction, which disturbs Sertoli cell function, and subsequently results in alterations in adult spermatogenesis. Birth Defects Res (Part B) 89:18-25, 2010. (c) 2009 Wiley-Liss, Inc.
Birth Defects Research Part B Developmental and Reproductive Toxicology 12/2009; 89(1):18-25. · 1.93 Impact Factor
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ABSTRACT: To explore the clinic characteristic and curative effect of staging tympanoplasty for chronic suppurative otitis media and cholesteatoma otitis media.
Curative effects of 132 patients cured by staging tympanoplasty and 325 patients cured by no staging tympanoplasty were retrospectively analyzed. Curative effects include ABG value (the reduced value of air-bone conduct gaps after operation), recrudescence of cholesteatoma, tympanic membrane reperforation and wet ear after operation.
In staging tympanoplasty group, ABG is 28.4 dB, rate of tympanic membrane reperforation is 1.5%, recrudescence of cholesteatoma is 0% and rate of wet ear after operation is 0%. In no staging tympanoplasty group, ABG is 21.3 dB, rate of tympanic membrane reperforation is 2.2%, recrudescence of cholesteatoma is 4.0% and rate of wet ear after operation is 1.2%.
The ABG, the rate of recrudescence of cholesteatoma in staging tympanoplasty group is much better than that in no staging tympanoplasty group. Tympanic membrane reperforation and wet ear after operation rate have no significant difference in statistic between two groups. We can conclude that staging operations will maintain and improve hearing level more effectively compare to no staging operation.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 06/2007; 21(10):441-3.
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ABSTRACT: To explore the effective treatment of juvenile outset recurrent respiratory papillomatosis (JO-RRP).
Thirty six patients of JO-RRP from 1993 to 2004 were analyzed retrospectively. Twenty seven patients were underwent laser excision by laryngoscope and nine patients were underwent laser excision through laryngostomy. Twenty eight patients who recurrenced more than two times in one year or involved extent of disease more than two anatomical subregion were give interferon after operation one week in addition.
All patients had normal deglutition, 28 cases (77.8%) had been succeed in decannulation. The complications included hoarseness in 8 cases, laryngeal stenosis in 6 cases and pulmonary infection in 3 cases.
The laser surgery by larynoscope and laryngostomy are two kinds of effective treatments for JO-RRP. The interferon therapy after the operation can restrain the recrudescence effectively.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 12/2006; 20(21):975-7.
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ABSTRACT: In patients of epilepsy a proportion of unexplained sudden deaths had been attributed to neurogenic arrhythmias. Although some evidence has suggested that epileptogenic activation of the cardiac parasympathetic nerves, which is revealed by ictal bradyarrhythmias or cardiac asystole, might be very critical in causing sudden deaths of patients of epilepsy the firing behavior of cardiac parasympathetic neurons (CPNs) during epileptic attack is not known. In the present study fluorescent tracer was injected into the cardiac sac of newborn rats to retrogradely label the parasympathetic neurons in the nucleus ambiguus (NA). The fluorescence-labeled NA neurons were further examined using whole-cell patch-clamp method in medulla slices with respiratory-like rhythm, and those with an inspiratory-related increase of the mixed inhibitory synaptic activity were identified as CPNs. We have demonstrated that blockade of the GABAergic and the glycinergic receptors in medulla slices evoked intermittent seizure-like firing of CPNs under current-clamp configuration, and evoked intermittent excitatory inward currents (IEICs) under voltage-clamp configuration. These results have given new evidence that CPNs might fire in a seizure-like pattern during epileptic attack, which might be responsible for the neurogenic ictal bradyarrhythmias, cardiac asystole, or even the sudden deaths of patients of epilepsy.
Epilepsy Research 09/2006; 70(2-3):172-83. · 2.29 Impact Factor
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ABSTRACT: To evaluate the clinical use of transiently evoked otoacoustic emissions (TEOAE) in patients with high-frequency sensorineural hearing loss, comparing the result of TEOAE with audiometry to find the correlation between them.
Fourteen normal adults and 25 patients with high-frequency hearing-loss were measured with TEOAE25 analyzer system and OB822 audiometer respectively, to compare the changing trend of the two examinations.
In the domain of 1.5 to approximately 4.5 kHz, there was significant difference of TEOAE between normal cases and hearing-loss cases. The results of TEOAE and audiometry had correlation with each other. The changing trend of the two examinations was accordant.
Combined with pure tone audiometry, TEOAE could discover the degree and range of high-frequency hearing loss more correctly.
Lin chuang er bi yan hou ke za zhi = Journal of clinical otorhinolaryngology 06/2005; 19(10):451-3.
