Abdullah Taskin

Harran University, Charan, Şanlıurfa, Turkey

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Publications (37)51.63 Total impact

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    ABSTRACT: The objective was to evaluate effects of dietary restriction on oxidative status and sperm parameters in rats exposed to long-term heat stress. Forty healthy Sprague-Dawley rats (2.5 months old) were equally allocated into 4 groups (with respect to diet and temperature): room temperature (22°C)-ad libitum; room temperature-dietary restriction (40%); high temperature (38°C)-ad libitum; and high temperature-dietary restriction. At the end of the 9th week, some oxidants (lipid hydroperoxide, total oxidant status, oxidative stress index) and some antioxidants (total antioxidant status, sulfhydryl groups, ceruloplasmin, paraoxonase and arylesterase activities) were measured in testicular tissues. In addition, concentration, motility, volume, abnormal sperm count, acrosome and membrane integrity of epididymal sperm were also evaluated. All data were analysed by 2-way ANOVA (P<0.05). High temperature did not significantly affect most oxidative and antioxidative parameters (except for sulfhydryl groups and ceruloplasmin), yet it impaired all sperm values. Neither sperm values nor oxidative status, with the exception of sulfhydryl groups, ceruloplasmin and arylesterase in the testis tissue, were significantly affected by dietary restriction. We concluded that long-term heat stress did not significantly affect testicular oxidative status in young rats, although sperm were sensitive to heat stress. Furthermore, dietary restriction failed to improve sperm quality and oxidative status, except some individual antioxidant parameters in young rats exposed to long-term heat stress.
    Reproduction Fertility and Development 12/2014; 27(1):225. · 2.58 Impact Factor
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    ABSTRACT: The aim of this study was to evaluate the protective activity of sildenafil treatment against ischemia-reperfusion damage created experimentally in rat ovaries. For this study, 42 female Wistar rats were used, and the rats were separated randomly into six groups consisting of seven rats each: sham, torsion, torsion-detorsion, torsion-detorsion + saline, torsion-detorsion + sildenafil 0.7 mg/kg and torsion-detorsion + sildenafil 1.4 mg/kg. With the exception of the sham group, an ovarian torsion procedure was implemented in all other groups for 2 h. Then, a detorsion procedure was implemented to the groups for 2 h, with the exception of the torsion group. Medications were given intraperitoneally, one-half hour before the detorsion procedure in the saline, 0.7 and 1.4 mg/kg sildenafil groups. Finally, 2 ml of blood samples was drawn for markers of oxidative stress, while the ovaries which were torsioned for the histological examination were extracted from all rats. According to the histopathological damage scores, the least damage was seen in the sham group and the most damage was seen in the torsion-detorsion group. The sildenafil treatment appeared to be effective in decreasing tissue damage; however, there were no differences between the dosages. Additionally, it was determined that the oxidative stress levels were higher in the torsion-detorsion group, while the sildenafil treatment caused a significant decrease in the oxidative stress levels. The results of the current study showed that the sildenafil treatment can be effective in preventing tissue damage and oxidative stress induced by the ischemia-reperfusion created in rat ovaries.
    Archives of Gynecology and Obstetrics 11/2014; · 1.33 Impact Factor
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    ABSTRACT: This study was conducted to evaluate the effects of dietary restriction on oxidative status and sperm parameters in rats exposed to long-term heat stress. Forty healthy Sprague–Dawley rats, aged 2.5 month, were divided into four groups of 10 with respect to feeding and temperature regimen (room temperature (22 °C)-ad libitum, room temperature–dietary restriction (40%), high temperature (38 °C)-ad libitum, high temperature–dietary restriction). At the end of the 9th week, some oxidants (lipid hydroperoxide, total oxidant status, oxidative stress index) and antioxidants (total antioxidant status, sulfhydryl groups, ceruloplasmin, paraoxonase and arylesterase activities) were measured in the testis tissue. The concentration, motility, volume, abnormal sperm count, acrosome and membrane integrity of epididymal spermatozoon and intratesticular testosterone levels were evaluated. High temperature did not change oxidative and antioxidative parameters except for sulfhydryl groups and ceruloplasmin, yet it impaired all sperm values. Neither sperm values nor oxidative status apart from sulfhydryl groups, ceruloplasmin and arylesterase was affected by dietary restriction in the testis tissue. These results suggest that long-term heat stress does not have a significant effect on testicular oxidative status, while the spermatozoa are sensitive to heat stress in young rats. Dietary restriction failed to improve the sperm quality and oxidative status except some individual antioxidant parameters; conversely, it decreased intratesticular testosterone level in the young rats exposed to long-term heat stress.
    Andrologia 11/2014; · 1.55 Impact Factor
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    ABSTRACT: Although placental abruption is an acute condition, it is thought that the underlying pathology is chronic vasculopathy. Collagen is one of the important components of vascular structure, and there is a correlation between collagen turnover and prolidase enzyme activity (PEA). Thus, our aim was to assess whether there is a difference in serum oxidative stress level and PEA between pregnant women with placental abruption and those with a healthy pregnancy.
    Archives of Gynecology and Obstetrics 09/2014; · 1.33 Impact Factor
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    ABSTRACT: Abstract Objective: In this experimental study, we investigated the possible effects of intracameral moxifloxacin on oxidative stress parameters and endothelial cell morphology in corneal tissue. Methods: In total, 30 rats were randomly assigned to three groups of 10 rats: the sham group (Group 1, n = 10); the control group (Group 2), where balanced salt solution (BSS) was administered at a dose of 0.01 cc (n = 10); and the treatment group (Group 3), where moxifloxacin was administered at a dose of 0.05 mg/0.01 cc (n = 10). Total antioxidant status (TAS) and total oxidant status (TOS) in corneal tissue and blood samples were measured and the oxidative stress index (OSI) was calculated. Also, corneal tissue histopathology was evaluated with caspase-3 and caspase-8 staining. Apoptotic activity was also evaluated. Results: In blood samples, TAS, TOS, and OSI levels were not statistically significantly different (all p > 0.05). Compared with the sham and control groups, TOS and OSI levels in cornea tissue were significantly different in the moxifloxacin group (all p < 0.05). However, compared with the control group, no statistically significant difference was found in the sham group (all p > 0.05). Compared with the sham and control groups, apoptotic activity was higher in the moxifloxacin group, in both immunohistochemical staining for caspase-3 and caspase-8. Conclusions: Intracameral moxifloxacin injection seems to be safe systemically, but it may have toxic effects on corneal tissues, as suggested by oxidative stress parameters and a histopathological evaluation.
    Cutaneous and ocular toxicology. 06/2014;
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    ABSTRACT: Carbon monoxide poisoning (COP) is an important cause of mortality and morbidity worldwide. This study was to investigate the levels of serum paraoxonase (PON), arylesterase (ARYL), ceruloplasmin (Cp), and sulfhydryl (-SH) in the treatment of COP, and to further understand the pathophysiology of COP.
    World journal of emergency medicine. 01/2014; 5(2):91-5.
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    ABSTRACT: Epilepsy is the most common neurologic disorder of childhood. In approximately 6-14% of all patients with epilepsy, complete seizure control is difficult to achieve with current antiepileptic treatments. Several current studies have shown in both animals and people that the lengthening of epileptic seizures and frequent recurrence increases the likelihood of neuronal damage. S-100B protein is the most analyzed brain derived peripheral biochemical marker in brain damage. This study aimed to evaluate interictal serum S-100B protein levels in children diagnosed with intractable epilepsy. A group of 32 patients with intractable epilepsy and 25 healthy controls were recruited. Serum S-100B protein levels were measured using a commercially available electrochemiluminescence immunoassay (ECLIA kit, as supplied and according to the manufacturer's standards. The serum S-100B protein levels of the patient group in the study were found to be 0.094±0.011 microgram/L, and 0.083±0.014microgram/L in the age-matched control group. The difference between the groups was determined to be statistically significant (P=0.004). In conclusions, it can be said that as the serum S-100B protein levels of the patients with focal epilepsy were high compared to those of the control group, this can be reliable peripheral biomarker for neuronal damage in patients with focal intractable epilepsy.
    Neuroscience Letters 11/2013; · 2.03 Impact Factor
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    ABSTRACT: Oxidative stress has been shown to play an important role in the pathogenesis of pre-eclampsia, and DNA damage frequently occurs in cells exposed to such stress. The aim of the present study was to investigate DNA damage and oxidative stress in mildly pre-eclamptic women and their offspring. We studied 25 mildly pre-eclamptic mothers, 20 healthy controls, and their infants. Mononuclear leukocyte DNA damage, total antioxidant status (TAS), and total oxidant status (TOS) were determined and the oxidative stress index (OSI) was calculated. DNA damage, and TOS and OSI levels were significantly increased, and TAS levels significantly decreased, in maternal and cord blood samples of the mildly pre-eclamptic group. A positive correlation between the extent of DNA damage and diastolic blood pressure was evident in pre-eclamptic mothers and there was a negative correlation between the extent of DNA damage and TOS. Both oxidative stress and DNA damage are elevated in mildly pre-eclamptic patients and their offspring. Increased oxidative stress may be important in inducing DNA damage in pre-eclamptic patients.
    European journal of obstetrics, gynecology, and reproductive biology 08/2013; · 1.97 Impact Factor
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    ABSTRACT: The aim of this study was to investigate the role of ischemic preconditioning (IPC) on ischemia/reperfusion (I/R)-induced injury of rat testis and determine the effects of 5-hydroxydecanoic acid (5-HD), a selective KATP channel antagonist, and Y-27632, a selective Rho kinase inhibitor, on IPC. I/R injury was induced by 180min ischemia and 60min reperfusion of testis. There were 5 groups. Group 1 served as untreated controls. The rats in Group 2 were subjected to I/R only. In Group 3, 3cycles of IPC (5min transient ischemia plus 5min reperfusion) were performed prior to I/R. In groups 4 and 5, the rats were treated as in Group 3 but received intraperitoneal injections of 0.3mg/kg Y-27632 or 10mg/kg 5-HD prior to IPC, respectively. I/R led to severe histopathological lesions in the rat testis and significantly lowered the scoring. I/R resulted in significant elevation in tissue lipid peroxide levels, myeloperoxidase (MPO) activity, and total antioxidative capacity (TAC), total oxidative status, and oxidative stress index levels. Protective effects of IPC on I/R-induced testicular injury of rats were observed with the significant recovery in these biochemical parameters. Y-27632 treatment led to a significant decrease in MPO activity, but there were no significant changes in the remaining parameters. Effects of IPC were blocked by 5-HD except in the TAC levels. Our results showed that IPC protected rat testis against I/R-induced injury via activation of KATP channels. Additionally, Rho kinase inhibition preserved the effects of IPC in testis.
    Journal of Pediatric Surgery 07/2013; 48(7):1565-72. · 1.38 Impact Factor
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    ABSTRACT: To evaluate the oxidative status following a seizure in children experiencing a simple febrile seizure. The cross-sectional study was conducted at Harran University, Turkey, between January and September 2011. It comprised 32 paediatric patients who, within the preceding 8 hours, had experienced a seizure due to upper respiratory tract infection and had been diagnosed with simple febrile seizure, and 30 healthy children as the control group. Blood was taken from the patients 8 hours after the seizure. Total oxidant level and Total anti-oxidant level were measured according to the Erel technique and the oxidative stress index was calculated. Data was analysed using SPSS 11.5. The mean values of the total oxidant level and the oxidative stress index of the cases were found to be significantly high compared to the controls and the total anti-oxidant level was found to be significantly low (p < 0.01, p < 0.01, p < 0.03 respectively). The increased total oxidant level and decreased total anti-oxidant level resulting in increased oxidative stress associated with febrile seizure patients may increase the risk of experiencing febrile seizures.
    Journal of the Pakistan Medical Association 05/2013; 63(5):594-7. · 0.41 Impact Factor
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    ABSTRACT: To determine the oxidative status and serum prolidase activity in tubal ectopic pregnancy and to see if there was any association between them. MATHODS: The cross-sectional study was conducted during 2009 and 2010 at the Departments of Obstetrics and Gynaecology and Clinical Biochemistry under the Faculty of Medicine, Harran University, Turkey. It comprised 40 patients with tubal ectopic pregnancies and 42 women with healthy pregnancies. Serum prolidase activity was measured spectrophotometrically. Oxidative status was determined using total antioxidant capacity. SPSS 11.5 was used for statistical analysis. Total antioxidant capacity levels were lower in the ectopic pregnancy group than the healthy group (p < 0.018), whereas total oxidant status, oxidative stress index and prolidase activity were higher (p < 0.05). Ectopic pregnancy may be associated with increased serum prolidase activity and oxidative stress, and this association may help to provide a better understanding about the pathogenesis of ectopic pregnancy.
    Journal of the Pakistan Medical Association 02/2013; 63(2):169-72. · 0.41 Impact Factor
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    ABSTRACT: Objective Regular physical activity is well known to play a cardioprotective role. The objective of this study was to investigate peripheral lymphocyte DNA damage and oxidative status in adult football players a three-day football tournament. Methods Twenty-five adult male football players and 25 sedentary male subjects were enrolled in the present study. Plasma total antioxidant status (TAS), total oxidant status (TOS) and the oxidative stress index (OSI) were determined. Peripheral lymphocyte DNA damage was determined using an alkaline comet assay. Results Plasma TOS, OSI and peripheral lymphocyte DNA damage were significantly lower in the adult football players than in the sedentary subjects (all: p<0.001), while TAS was significantly higher in the football players (p<0.001). The plasma TAS levels were inversely correlated with TOS, OSI and peripheral lymphocyte DNA damage (r =-0.683, p<0.001; r =-0.909, p<0.001; r =-0.608, p<0.001; respectively) in the adult football players. Conclusion These results indicate that physical activity is associated with increased antioxidant capacity and decreased oxidative stress. Such conditions are important for a healthy life. Further studies are needed to clarify the mechanisms underlying this association.
    Internal Medicine 01/2013; 52(2):213-7. · 0.97 Impact Factor
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    ABSTRACT: BACKGROUND: Breath-holding spells (BHS) are the most common form of non-epileptic paroxysmal events in infancy. The pathophysiology of BHS is not fully understood. Iron-deficiency anemia (IDA) may be a factor contributing to breath-holding spells. Although numerous reports have shown that elevated oxidative stress is implicated in the pathophysiology of neurodegenerative diseases and neurological conditions, such as epileptic seizures, brain damage, and neurotrauma, there are no data regarding the role of oxidative stress in the development of BHS. This study aimed to investigate oxidative stress in children with BHS. METHODS: This case-control study was conducted at the Department of Pediatric Neurology, Harran University School of Medicine, Sanliurfa, in Turkey. Blood samples from 31 patients (14 females, 17 males) with BHS which were taken at least 24 h after the BHS attack, and a control group of 35 healthy individuals (13 females, 22 males) were used for the measurement of the plasma total antioxidant capacity, total oxidant status, and oxidative stress index, hemoglobin concentration, serum iron, transferrin saturation and serum ferritin levels. RESULTS: The plasma total antioxidant capacity values were markedly lower and total oxidant status and oxidative stress index values in the BHS group were significantly higher than that in the controls (P ≤ 0.01). CONCLUSION: Our data suggest that the value of oxidative stress was significantly higher in patients with BHS than in the controls. Conditions associated with increased oxidative stress such as IDA may be a risk factor for the development of BHS.
    Child s Nervous System 12/2012; · 1.24 Impact Factor
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    ABSTRACT: PURPOSE: An elevated level of S-100B in serum is generally considered to be a biochemical marker of nervous tissue damage. According to our knowledge, no studies have evaluated the serum S-100B protein concentration in children with temporal lobe epilepsy. The objective of this study was to measure the serum levels of S-100B protein in pediatric cases with temporal epilepsy. METHODS: This case-controlled cross-sectional study was performed at the Department of Pediatric Neurology, Harran University School of Medicine, Sanliurfa, in Turkey. Serum S-100B protein levels were studied in 19 (12 females, 7 males) children with temporal lobe epilepsy and in 25 (15 females, 10 males) healthy control subjects. Serum samples were collected within 30min after a complex partial seizure, and serum S-100B protein levels were measured with an electrochemiluminescence immunoassay for the quantification of protein (ECLIA kit, Roche(®) Diagnostics, Germany). RESULTS: The mean serum concentration of S-100B protein was 0.12±0.02μg/L in the temporal lobe epilepsy group and 0.07±0.01μg/L in the control group. The patients showed significantly elevated S-100B protein levels compared with healthy controls (P<0.001). CONCLUSION: Our data suggest that increased S-100B protein levels in the serum might reflect neuronal damage in the brains of children with temporal lobe epilepsy. These results do confirm the previous findings of elevated S-100B protein levels in adult patients with temporal lobe epilepsy.
    Seizure 11/2012; · 2.00 Impact Factor
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    ABSTRACT: OBJECTIVES: Tension-type headache (TTH) and fibromyalgia syndrome (FM) are worldwide seen chronic pain syndromes of unknown etiology. Despite the growing body of data on pathophysiology and generation mechanisms of pain; our knowledge on pain mechanisms in both FM and TTH is yet to be limited. PATIENTS AND METHODS: We investigated the plasma nitrite levels, total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) in 35 TTH, 33 FM patients and 31 healthy controls. RESULTS: The mean plasma nitrite levels and TAS levels were significantly low and OSI was found to be significantly high in TTH and FM groups compared to the control group (p=0.001, p=0.001, p=0.001 and p=0.001, respectively). The mean serum TOS levels were also significantly higher in FM group according to the control group (p=0.034), but there was not a significant difference between TTH and control groups (p=0.066). CONCLUSION: These results indicated that; FM and TTH patients revealed higher oxidative stress index and lower total nitrite levels than healthy controls. We conclude that oxidative stress may have a role in the pathophysiological mechanisms of TTH and FM, although, whether it is the cause or the consequence, is not clear.
    Clinical neurology and neurosurgery 10/2012; · 1.30 Impact Factor
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    ABSTRACT: Hepatitis C infection represents a common healthcare issue worldwide. The present trial was designed to investigate the role of prolidase, an enzyme that is significantly involved in the biosynthesis of collagen, and of the oxidative stress that is considered to be involved in the pathogenesis of various diseases, in the chronic hepatitis C infection. The trial was performed to assess the serum prolidase enzyme level and the oxidative-antioxidative status and to determine the relation between the serum prolidase activity and the oxidative stress parameters. A total of 95 individuals, including 55 patients with chronic hepatitis C infection (CHC) and 40 healthy adults, were enrolled in the trial. The values for prolidase, the total antioxidant status (TAS), the total oxidative stress (TOS), the oxidative stress index (OSI), sulfhydryl (SH), lipid peroxidation LOOH, catalase (CAT), and ceruloplasmin were measured and compared between the patient groups. The prolidase, TOS, LOOH, CAT, and the OSI values were higher in the chronic hepatitis C group compared to the control group (P < 0.001). The TAS, SH, and ceruloplasmin levels were lower in the CHC group relative to the control group (P < 0.001). We suppose that the values of prolidase and the oxidative stress are increased while the antioxidant levels are decreased in CHC. As a result, prolidase and the oxidative stress seem to be related with the progression of the disease.
    Journal of Clinical Laboratory Analysis 07/2012; 26(4):232-7. · 1.36 Impact Factor
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    ABSTRACT: The aim of this study was to evaluate oxidative stress in various clinical forms of hepatitis B infection and to investigate its role in the development of the chronic form of the disease. Ninety-three patients with inactive hepatitis B surface antigen (HbsAg) carrier state (IHBCS), 65 patients with chronic hepatitis B infection (CHB), and 42 healthy adults were included in the study. The following values were measured and compared in patient groups: total antioxidant status (TAS), total oxidative stress (TOS), oxidative stress index (OSI), sulfhydryl (SH), lipid peroxidation (LOOH), catalase (CAT), and ceruloplasmin. In patients with chronic hepatitis B, these values were compared with HBV DNA and fibrosis levels. ALT, TOS, LOOH, and OSI levels were higher in the CHB group compared to the other groups (P<0.001). Catalase levels increased in the CHB and IHBCS groups compared to the control group (P<0.001). Total aminooxidant and ceruloplasmin levels were found to be lowest in the CHB group and highest in the control group (P<0.001). Sulfhyrdyl was higher in the control group compared to the other groups (P<0.001). In the CHB group, there was no correlation between the HBV DNA and OSI (P>0.05). These finding suggested that oxidative stress is associated with hepatitis B activity.
    Annals of Laboratory Medicine 03/2012; 32(2):113-8. · 1.48 Impact Factor
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    ABSTRACT: To investigate the relationship between level of sperm DNA damage, seminal oxidative status, and shock-wave lithotripsy (SWL) for distal ureteral stones. Prospective study with control. Academic research institute. Men who had undergone SWL for distal and upper ureter stones. Level of sperm DNA damage and seminal oxidative status assessed through the examination of semen on the day before and 3 days, and 3 months after SWL. DNA damage score and oxidative stress in semen parameters. Sperm DNA damage score and semen total oxidant status (TOS) levels increased, but semen total antioxidant status (TAS) levels, sperm concentration, and motility decreased immediately after SWL. However, there were no statistically significant correlations between the DNA damage scores and the increased TAS and TOS levels in the study group. All of the changes returned completely to initial level during three months after SWL. SWL may affect fertility in men. Therefore, we suggest other treatment modalities, such as ureteroscopy, for young men with distal ureteral stones to prevent the development of male infertility.
    Fertility and sterility 08/2011; 96(5):1087-90. · 3.97 Impact Factor
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    ABSTRACT: The aim of this study is to evaluate the relationship between cord blood oxidative and antioxidative status and maternal parity number. Patients are grouped according to the maternal parity number: primiparous group (n = 36), multiparous group (n = 40), and grand multiparous group (n = 26). Cord blood samples are obtained in all subjects and assessed for total antioxidant capacity (TAC), total oxidant status (TOS) and oxidative stress index (OSI). The serum TAC and TOS were evaluated by using an automated colorimetric measurement method. TAC levels are significantly higher and oxidative stress indicators are significantly lower in newborns of primiparous women compared to multiparous women p < 0.05 for all). TAC level is increased, whereas TOS and OSI levels are decreased in newborns of grand multiparous mothers compared to primiparous mothers. TAC level is significantly higher, whereas TOS and OSI levels are significantly lower in newborns of grand multiparous women compared to multiparous women (p < 0.05 for all). These results suggest a relation between higher maternal parity and increased oxidative stress and decreased antioxidant defense capacity. On the other hand, the compensatory mechanisms improve the antioxidant defense system in newborns of grand multiparous women and may prevent oxidative stress.
    The journal of maternal-fetal & neonatal medicine: the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians 08/2011; 25(6):802-5. · 1.36 Impact Factor