Natalie Trachtmann

Universität Stuttgart, Stuttgart, Baden-Württemberg, Germany

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Publications (5)20.98 Total impact

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    ABSTRACT: Biosynthesis as a model: Based on the branched structure of biosynthetic pathways, such as the shikimate pathway, the selective bioproduction of a set of diverse metabolites has been achieved by means of metabolic engineering (see scheme). A scale-up for preparative purposes was performed, resulting in high product titers and yields from renewable resources.
    Angewandte Chemie International Edition 08/2011; 50(34):7781-6. DOI:10.1002/anie.201103261 · 11.26 Impact Factor
  • Christoph Albermann · Natalie Trachtmann · Georg A Sprenger ·
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    ABSTRACT: We report a method for the integration of expression cassettes into the Escherichia coli chromosome using rare and dispensable sugar degradation gene loci as sites for integration. Clones carrying successfully recombined DNA fragments in the chromosome are easily screened using a solid differential medium containing the respective sugar compound. As an example for the heterologous expression of a complex natural product biosynthesis pathway, we show the stepwise chromosomal integration of the zeaxanthin biosynthesis pathway from Pantoea ananatis into E. coli.
    Biotechnology Journal 01/2010; 5(1):32-8. DOI:10.1002/biot.200900193 · 3.49 Impact Factor
  • R. Feuer · M. Ederer · N. Trachtmann · T. Sauter · E.D. Gilles · G. Sprenger · O. Sawodny ·
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    ABSTRACT: Mathematical models of the metabolism of organisms like Escherichia coli provide a possibility to improve the production of industrial relevant compounds. These models allow predictions of the behavior of metabolism and thus proposals to change it to an industrial benefit. We use an innovative approach utilizing evolutionary adaptation for the activation of relevant biosynthesis pathways. The evolutionary trend of E. coli to optimal substrate yields is employed to maximize the production of an industrial desired compound. Pyruvate is a central metabolite in metabolism that is essential for survival. Under an evolutionary pressure an E. coli mutant with knock outs in the main pyruvate synthesis pathways deregulates alternative pyruvate synthesis pathways. Many of those alternative pathways operate via industrial relevant compounds. Thus their deregulation is an important step towards efficient production strains. This paper uses a metabolic network model and presents a method to predict possible deregulated pathways. Furthermore tools to analyze the pathways and to support their understanding are presented.
    SICE, 2007 Annual Conference; 10/2007
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    ABSTRACT: Rhodococcus (opacus) erythropolis HL PM-1 grows on 2,4,6-trinitrophenol or 2,4-dinitrophenol (2,4-DNP) as a sole nitrogen source. The NADPH-dependent F420 reductase (NDFR; encoded by npdG) and the hydride transferase II (HTII; encoded by npdI) of the strain were previously shown to convert both nitrophenols to their respective hydride Meisenheimer complexes. In the present study, npdG and npdI were amplified from six 2,4-DNP degrading Rhodococcus spp. The genes showed sequence similarities of 86 to 99% to the respective npd genes of strain HL PM-1. Heterologous expression of the npdG and npdI genes showed that they were involved in 2,4-DNP degradation. Sequence analyses of both the NDFRs and the HTIIs revealed conserved domains which may be involved in binding of NADPH or F420. Phylogenetic analyses of the NDFRs showed that they represent a new group in the family of F420-dependent NADPH reductases. Phylogenetic analyses of the HTIIs revealed that they form an additional group in the family of F420-dependent glucose-6-phosphate dehydrogenases and F420-dependent N5,N10-methylenetetrahydromethanopterin reductases. Thus, the NDFRs and the HTIIs may each represent a novel group of F420-dependent enzymes involved in catabolism.
    Applied and Environmental Microbiology 06/2003; 69(5):2748-54. DOI:10.1128/AEM.69.5.2748-2754.2003 · 3.67 Impact Factor
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    ABSTRACT: Rhodococcus (opacus) erythropolis HL PM-1 grows on 2,4,6-trinitrophenol (picric acid) or 2,4-dinitrophenol (2,4-DNP) as sole nitrogen source. A gene cluster involved in picric acid degradation was recently identified. The functional assignment of three of its genes, npdC, npdG and npdI, and the tentative functional assignment of a fourth one, npdH, is reported. The genes were expressed in Escherichia coli as His-tag fusion proteins that were purified by Ni-affinity chromatography. The enzyme activity of each protein was determined by spectrophotometry and HPLC analyses. NpdI, a hydride transferase, catalyses a hydride transfer from reduced F420 to the aromatic ring of picric acid, generating the hydride sigma-complex (hydride Meisenheimer complex) of picric acid (H(-)-PA). Similarly, NpdI also transformed 2,4-DNP to the hydride sigma-complex of 2,4-DNP. A second hydride transferase, NpdC catalysed a subsequent hydride transfer to H(-)-PA, to produce a dihydride sigma-complex of picric acid (2H(-)-PA). All three reactions required the activity of NpdG, an NADPH-dependent F420 reductase, for shuttling the hydride ions from NADPH to F420. NpdH converted 2H(-)-PA to a hitherto unknown product, X. The results show that npdC, npdG and npdI play a key role in the initial steps of picric acid degradation, and that npdH may prove to be important in the later stages.
    Microbiology 04/2002; 148(Pt 3):799-806. DOI:10.1099/00221287-148-3-799 · 2.56 Impact Factor