Jeng-A Han

Publications (2)6.38 Total impact

• Article: Functional study of Villin 2 protein expressed in longissimus dorsi muscle of Korean native cattle in different growth stages.

  Yong-Cheng Jin, Jeng-A Han, Cheng-Xiong Xu, Sang-Kee Kang, Sang-Hun Kim, Kang-Suk Seo, Du-Hak Yoon, Yun-Jaie Choi, Hong-Gu Lee
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  ABSTRACT: The aim of this study was to investigate protein profiles related to the induction of adipogenesis within the bovine longissimus dorsi muscle (BLDM) by proteomic analysis. We analyzed BLDM proteins at different growth stages to clarify the physiological mechanisms of marbled muscle development in 20 head of Korean native cattle (11 month: 10 head, 17 month: 10 head). BLDM proteins were analyzed by two-dimensional electrophoresis and image analysis. Villin 2 was specifically identified by mass spectrometry and a protein search engine. Villin 2 protein expression in BLDM decreased during the fat development stage in test steers. In a Western blot cell culture study of spontaneously immortal bovine muscle fibroblasts, the abundance of Villin 2 was shown to be down-regulated during differentiation into muscle. In 3T3-L1 mouse embryonic fibroblasts, Villin 2 was decreased during differentiation into adipocytes. The results suggest that Villin 2 may be related to the induction of transdifferentiation and adipogenesis in bovine longissimus dorsi muscle.
  BMB reports 02/2012; 45(2):102-7. · 1.72 Impact Factor
• Source

  Available from: Seong Ho Choi

  Article: Proteomic analysis of endogenous conjugated linoleic acid biosynthesis in lactating rats and mouse mammary gland epithelia cells (HC11).

  Yong-Cheng Jin, Hong-Gu Lee, Cheng-Xiong Xu, Jeng-A Han, Seong-Ho Choi, Man-Kang Song, Young-Jun Kim, Ki-Beom Lee, Seon-Ku Kim, Han-Seok Kang, Byung-Wook Cho, Teak-Soon Shin, Yun-Jaie Choi
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  ABSTRACT: This study was conducted to investigate the amount of CLA synthesized endogenously by rat mammary tissues in response to TVA (a precursor for cis-9, trans-11 CLA endogenous synthesis) treatment as well as the differences in the protein expression of genes encoding the biosynthesis of CLA in rat mammary tissue and mouse mammary gland epithelia cells (HC11). Treatment with TVA resulted in improved CLA productivity. Furthermore, 2-DE revealed two spots in samples of mammary tissues and one spot in samples of mammary gland epithelia cells (HC11) that were consistently altered in the TVA treatment groups when compared with the control group (non-fatty acid). The mRNA expression patterns of three of the proteins (PDI, PRDX2, LAMR1), as measured by real-time PCR, were similar to the pattern of protein abundance. In addition, the expression of SCD mRNA in the mammary tissue of rats and HC11 cell treated with TVA was higher than in the control group. Our results suggest that the identified proteins may be related to CLA biosynthesis in mammary tissue.
  Biochimica et Biophysica Acta 11/2009; 1804(4):745-51. · 4.66 Impact Factor