Publications (2)1.53 Total impact
Article: [Hepatitis A virus detection in shellfish from Tunisia by reverse transcription-nested PCR--investigation of a correlation between viral and bacterial contamination].[show abstract] [hide abstract]
ABSTRACT: We aimed at evaluating the contamination by hepatitis A virus (HAV) of 54 shellfish samples collected from five Tunisian shellfish harvesting areas and finding a correlation between bacterial and viral contamination. Fifty-four shellfish samples were analysed in our study. Two methods of viral extraction were evaluated by reverse transcription-nested PCR. The first one was based on elution by glycine solution and the second one used a beef extract solution. Bacteriological determination (Samonella and E. coli) was carried out for all shellfish samples. Glycine extraction showed a higher detection rate of HAV compared to the saline beef extraction method. The hepatitis A virus was detected in 32 % of shellfish samples analysed. None of the samples revealed the presence of Samonella. From 17 samples positive for HAV, we found six samples showing a number of E. coli below the European legislation. An important HAV contamination was observed in our study. No correlation between bacterial and viral contamination was found.Pathologie Biologie 11/2009; 59(4):217-21. · 1.53 Impact Factor
Article: De´ tection du virus de l’he´ patite A dans les coquillages en Tunisie par reversetranscription-nested PCR – recherche de corre´ lation entre la contamination viraleet bacte´ riennePathologie Biologie. 01/2009; doi:10.1016/j.patbio.2009.10.009.