Publications (3)4.23 Total impact
Article: Rhizobium tarimense sp. nov. isolated from soil in the ancient Khiyik river of Xinjiang, China.[show abstract] [hide abstract]
ABSTRACT: A Gram-negative, non-motile, pale-yellow, rod-shaped bacterial strain PL-41T, was isolated from Populus euphratica forest soil at the ancient Khiyik River valley in Xinjiang Uyghur Autonomous Region, People's Republic of China. Strain PL-41T grew optimally at 30 °C and pH 7.0-8.0. The major quinone was Q-10. The predominant cellular fatty acids of strain PL-41T were summed feature 8 (comprising C18:1ω7c and C18:1ω6c), C16:0 and C19:0 cyclo ω8c. Polar lipids of strain PL-41T include two unidentified aminophospholipids (APL1, 2), two unidentified phospholipids (PL1, 2), phosphatidylcholine and three unidentified lipids (L1-3). Strain PL-41T showed 16S rRNA gene sequence similarity of 97.0-97.5 % to the type strains of recognized Rhizobium species. Phylogenetic analysis of strain PL-41T based on the sequences of housekeeping genes recA and atpD confirmed (similarities are less than 90 %) its position as a distinct Rhizobium species. The DNA G+C content was 57.8 mol %. DNA-DNA relatedness between strain PL-41T and the type strains of Rhizobium huautlense S02T, Rhizobium alkalisoli CCBAU 01393T, Rhizobium vignae CCBAU 05176T and Rhizobium loessense CCBAU 7190BT were 33.4, 22.6, 25.5 and 45.1 %, respectively, indicating that strain PL-41T was distinct from them genetically. Strain PL-41T also can be differentiated from these four phylogenetically related Rhizobium species by various phenotypic properties. On the basis of phenotypic properties, phylogenetic distinctiveness and genetic data, strain PL-41T is considered to represent a novel species of the genus Rhizobium, for which the name Rhizobium tarimense sp. nov. is proposed. The type strain is PL-41T (=CCTCC AB 2011011T = NRRL B-59556T).INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2012; · 2.11 Impact Factor
Article: [Phylogenetic diversity of culturable endophytic bacteria isolated from the Populus euphratica at the disused ancient Ugan river].[show abstract] [hide abstract]
ABSTRACT: The aim of this study was to identify the culturable endophytic bacteria recovered from the Populus euphratica at the disused (122 years ago) ancient Ugan river of middle reachs of Tarim river, and to understand their Phylogenetic diversity and community structure. Bacteria were isolated from the storage liquid in the stem of 2 Populus euphratica stands by using 3 types of different cultural medium (Luria-Bertani, Trypticase Soy Agar and Nutrient Agar), followed carry out 16S rDNA identifications and analysis of their biodiversity. A total of 62 phenotypically different isolates were sequenced and according to their 16S rDNA sequence similarities to type strains of described organisms, they have been placed into four phylogenetic groups (1, Firmicutes; 2, Actinobacteria; 3, Alpha Proteobacteria and 4, Gamma Proteobacteria), 18 genera and 32 species. Among them, Bacillus and pseudomonas were the most widely distributed and predominant, occupied the majority of isolates 40.32% and 16.13%, respectively. Isolate KTH-63 (HM371419) formed a distinct clade with Macrococcus brunensis in phylogenetic tree based on 16S rDNA sequence among the family Staphylococcaceae, so it was demonstrated that the KTH-63 represents a potential novel genus and novel species within the family Staphylococcaceae with 92.491% sequence similarity with the described species Macrococcus brunensis of this family. Isolates KLH-1, KLH-21, KLH-18, KLH-25, KNA-3, KTH-8, KTH-14, KTH-20 and KNA-26 with 96.089% - 97.769% sequence similarities to their closely related members were presumed to be potential novel species, and the discovery rate of potential novel species in the endophytic bacterial community of Populus euphratica was reach up to 16.13%. Furthermore, 10 genera and 18 species have been added to the plant endophytic bacterial categoria by the data obtained in this work. The result showed that the cultivable endophytic bacterial diversity in Populus euphratica at Ugan river was very abundant and have high percentage of potential novel species, and it have greatly refreshed the plant endophytic bacterial records. The community structure obtained in this study also may be presumed as a miniature of the endophytic bacterial flora in the Populus euphratica during the recent ages before the affect of modern civilization prossessing to Tarim river valley, which deserve further study and exploitation.ACTA MICROBIOLOGICA SINICA 02/2011; 51(2):178-88.
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ABSTRACT: A Gram-staining-negative, yellow-coloured, strictly aerobic, non-spore-forming, rod-shaped bacterium, designated HS39(T), isolated from a soil sample collected from a natural Populus euphratica forest in Xinjiang, China, was characterized using a polyphasic approach. The isolate grew optimally at 30-37 °C, at pH 6.5-8.0 and with 0-3 % NaCl. Analysis of the 16S rRNA gene sequence of strain HS39(T) revealed that it is a member of the genus Sphingobacterium. Sphingobacterium mizutaii ATCC 33299(T) was the nearest relative (94.0 % 16S rRNA gene sequence similarity). The G+C content of the genomic DNA was 40.2 mol%. The major fatty acids were iso-C₁₅:₀, iso-C₁₇:₀ 3-OH and summed feature 3 (comprising C₁₆:₁ω6c and/or C₁₆:₁ω7c). The predominant isoprenoid quinone was MK-7. On the basis of phenotypic properties and phylogenetic inference, strain HS39(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium shayense sp. nov. is proposed. The type strain is HS39(T) (=CCTCC AB 209006(T) =NRRL B-59203(T)).INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2009; 60(Pt 10):2377-81. · 2.11 Impact Factor