George Yeung

Publications (2)67.48 Total impact

• Article: Accurate whole-genome sequencing and haplotyping from 10 to 20 human cells.

  Brock A Peters, Bahram G Kermani, Andrew B Sparks, Oleg Alferov, Peter Hong, Andrei Alexeev, Yuan Jiang, Fredrik Dahl, Y Tom Tang, Juergen Haas, [......], Kaliprasad Pothuraju, Karel Konvicka, Mike Tsoupko-Sitnikov, Krishna P Pant, Jessica C Ebert, Geoffrey B Nilsen, Jonathan Baccash, Aaron L Halpern, George M Church, Radoje Drmanac
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  ABSTRACT: Recent advances in whole-genome sequencing have brought the vision of personal genomics and genomic medicine closer to reality. However, current methods lack clinical accuracy and the ability to describe the context (haplotypes) in which genome variants co-occur in a cost-effective manner. Here we describe a low-cost DNA sequencing and haplotyping process, long fragment read (LFR) technology, which is similar to sequencing long single DNA molecules without cloning or separation of metaphase chromosomes. In this study, ten LFR libraries were made using only ∼100 picograms of human DNA per sample. Up to 97% of the heterozygous single nucleotide variants were assembled into long haplotype contigs. Removal of false positive single nucleotide variants not phased by multiple LFR haplotypes resulted in a final genome error rate of 1 in 10 megabases. Cost-effective and accurate genome sequencing and haplotyping from 10-20 human cells, as demonstrated here, will enable comprehensive genetic studies and diverse clinical applications.
  Nature 07/2012; 487(7406):190-5. · 36.28 Impact Factor
• Source

  Available from: genome.gov

  Article: Human genome sequencing using unchained base reads on self-assembling DNA nanoarrays.

  Radoje Drmanac, Andrew B Sparks, Matthew J Callow, Aaron L Halpern, Norman L Burns, Bahram G Kermani, Paolo Carnevali, Igor Nazarenko, Geoffrey B Nilsen, George Yeung, [......], Dylan Vu, Alexander Wait Zaranek, Xiaodi Wu, Snezana Drmanac, Arnold R Oliphant, William C Banyai, Bruce Martin, Dennis G Ballinger, George M Church, Clifford A Reid
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  ABSTRACT: Genome sequencing of large numbers of individuals promises to advance the understanding, treatment, and prevention of human diseases, among other applications. We describe a genome sequencing platform that achieves efficient imaging and low reagent consumption with combinatorial probe anchor ligation chemistry to independently assay each base from patterned nanoarrays of self-assembling DNA nanoballs. We sequenced three human genomes with this platform, generating an average of 45- to 87-fold coverage per genome and identifying 3.2 to 4.5 million sequence variants per genome. Validation of one genome data set demonstrates a sequence accuracy of about 1 false variant per 100 kilobases. The high accuracy, affordable cost of $4400 for sequencing consumables, and scalability of this platform enable complete human genome sequencing for the detection of rare variants in large-scale genetic studies.
  Science 11/2009; 327(5961):78-81. · 31.20 Impact Factor