R A Yurasov

Publications (3)5.91 Total impact

• Source

  Available from: Vladimir R Chechetkin

  Article: Kinetic effects on signal normalization in oligonucleotide microchips with labeled immobilized probes.

  S V Pan'kov, V R Chechetkin, O G Somova, O V Antonova, O V Moiseeva, D V Prokopenko, R A Yurasov, D A Gryadunov, A V Chudinov
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  ABSTRACT: Among various factors affecting operation of oligonucleotide microchips, the variations in concentration and in homogeneous distribution of immobilized probes over the cells are one of the most important. The labeling of immobilized probes ensures the complete current monitoring on the probe distribution and is reliable and convenient. Using hydrogel-based oligonucleotide microchips, the applicability of Cy3-labeled immobilized probes for quality control and signal normalization after hybridization with Cy5-labeled target DNA was investigated. This study showed that proper signal normalization should be different in thermodynamic conditions and in transient regime with hybridization far from saturation. This kinetic effect holds for both hydrogel-based and surface oligonucleotide microchips. Besides proving basic features, the technique was assessed on a sampling batch of 50 microchips developed for identifying mutations responsible for rifampicin and isoniazid resistance of Mycobacterium tuberculosis.
  Journal of biomolecular structure & dynamics 11/2009; 27(2):235-44. · 4.99 Impact Factor
• Article: Biological microchip for a simultaneous quantitative immunoassay of tumor markers in human serum.

  E N Savvateeva, E I Dementieva, M V Tsybulskaya, T V Osipova, T P Ryabykh, A Yu Turygin, R A Yurasov, A S Zasedatelev, A Yu Rubina
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  ABSTRACT: The biochip was constructed for simultaneous assay of total and free prostate-specific antigen, alpha-fetoprotein, cancer embryonic antigen, human chorionic gonadotropin, and neuron-specific enolase. These biochips represent an array of gel elements with covalently immobilized proteins. The major analytic characteristics of the developed method were obtained. It was shown that the results of simultaneous assay of six tumor markers in blood serum well correlated with routine measurement of each marker using enzyme immunoassay kits. This approach allowed us to reveal the hook effect of high concentrations during biochip assay, which prevents distortion of the diagnostic picture at high concentration of the analyte in the sample.
  Bulletin of Experimental Biology and Medicine 06/2009; 147(6):737-41. · 0.27 Impact Factor
• Source

  Available from: Denis O Fesenko

  Article: Alginate gel biochip for real-time monitoring of intracellular processes in bacterial and yeast cells

  D. O. Fesenko, T. V. Nasedkina, A. V. Chudinov, D. V. Prokopenko, R. A. Yurasov, A. S. Zasedatelev
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  ABSTRACT: A method was developed for producing cell biochips on the basis of calcium alginate. Cell immobilization in microvolumes of nontoxic alginate gel under mild conditions extended the range of testable micro-organisms. The possibility of studying the intracellular processes with alginate gel biochips was demonstrated in model experiments with Escherichia coli, Bordetella bronchiseptica, and Saccharomyces cerevisiae. Cell biochips proved to be suitable for simultaneous monitoring of nucleic acid and protein syntheses with two fluorescent dyes. The effect of chloramphenicol on nucleic acid synthesis was studied with five bacterial strains. Inducible synthesis of the green fluorescence protein (EGFP) in E. coli cells was monitored with the use of biochips. The level of EGFP synthesis correlated with the inductor concentration in the medium.
  Molecular Biology 12/2004; 39(1):84-89. · 0.66 Impact Factor