Nurit Golenberg

Publications (2)5.62 Total impact

• Article: Acute repair of chondrocytes in the rabbit tibiofemoral joint following blunt impact using P188 surfactant and a preliminary investigation of its long-term efficacy.

  Daniel I Isaac, Nurit Golenberg, Roger C Haut
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  ABSTRACT: Recent studies have indicated that there may be a correlation between acute chondrocyte damage and joint degeneration reminiscent of early-stage osteoarthritis (OA). P188 surfactant has been shown to acutely restore the integrity of damaged chondrocytes; however, its long-term efficacy is unknown. The hypothesis of this study was that a single injection of P188 into a traumatized joint would acutely repair damaged cell membranes and maintain their viability in the long term. Twelve rabbits were divided into two groups, with and without P188, and sacrificed 4 days after tibiofemoral (TF) impact. Another six rabbits were sacrificed after 6 weeks and divided into two groups, with and without P188 treatment immediately posttrauma. Treatment with P188 increased the viable cell density 4 days posttrauma. A higher density of viable cells was also documented 6 weeks posttrauma in the treated versus untreated limb. The results of the current study confirm the acute efficacy of P188 treatment, and may suggest long-term efficacy of treatment, but additional studies are still needed to investigate the chronic implications of the acute repair of cells in the traumatized joint.
  Journal of Orthopaedic Research 10/2009; 28(4):553-8. · 2.81 Impact Factor
• Article: Effect of intermittent cyclic preloads on the response of articular cartilage explants to an excessive level of unconfined compression.

  Feng Wei, Nurit Golenberg, Eugene T Kepich, Roger C Haut
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  ABSTRACT: Mechanical loading of articular cartilage can influence chondrocyte metabolism and lead to alterations in cartilage matrix composition. Most previous studies have focused on the effect of cyclic loading on cartilage mechanical properties and proteoglycan synthesis. However, the role of proteoglycans synthesized from cyclically loaded cartilage in response to an acute overload has not been elucidated. Therefore, we conducted studies where low intensity, intermittent cyclic loading was applied to chondral explants prior to an acute unconfined compression on the tissue. The chondral explants were randomly assigned to three groups: 7, 14, and 21 days of 10 cycles of 0.2 Hz sinusoidal loading at 0.5 MPa followed by an unloaded interval of 3,600 s. All explants were then taken to 25 MPa of unconfined compression. Biochemical assays were conducted to determine the tissue proteoglycan and hydroxyproline contents. The results showed cyclic preloading increased the proteoglycan content and mechanically stiffened the explants, making them more resistant to matrix damage and cell death under 25 MPa of unconfined compression up to 14 days. After 21 days of cyclic loading, however, the explants lost compressive stiffness and suffered more extensive damage in the unconfined compression test. This study investigated the role of cyclic loading in response of chondral explants to a potentially damaging, acute overload. In the long term, these types of studies may help understand the role of preconditioning of articular cartilage for in vitro or even in vivo studies of blunt force trauma to a joint.
  Journal of Orthopaedic Research 12/2008; 26(12):1636-42. · 2.81 Impact Factor