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ABSTRACT: In the present paper, a method was established based on laser tweezer Raman spectroscopy for rapid quantification of astaxanthin in Pha F Fia Rhodozyma cells. First, the Raman spectra of astaxanthin standard solution with different concentrations were determined and the standard curve for astaxanthin with the peak intensity at 1 520 cm- was plotted; And then the Pha F Fia yeast cells cultivated in different nitrogen source and carbon source medium were divided into two parts, one for the detection of Raman spectra, and the other for the determination of ultraviolet visible spectrophotometry; Finally the relationship between the two methods was analyzed. The correlation coefficient of standard curve for astaxanthin is 0.998 3. Comparing laser tweezers Raman spectroscopy method with traditional ultraviolet visible spectrophotometry in analyzing the content of astaxanthin in unit mass Pha F Fia rhodozyma and the yield of astaxanthin in unit volume fermentation broth of Pha F Fia rhodozyma, the authors found that the data obtained have good linear relationship. And the correlation coefficients are 0.917 7 and 0.905 4, respectively. Therefore, both methods have almost the same effect of measuement. But laser tweezers Raman spectroscopy method is more efficient in the quantitative analysis of astaxanthin in Pha F Fia Rhodozyma cells.
Guang pu xue yu guang pu fen xi = Guang pu 09/2012; 32(9):2433-7. · 0.84 Impact Factor
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ABSTRACT: Zinc is a trace metal and acts as an active component of various enzymes. Zinc deficiency has been suggested to be associated with the development of diabetes. The present study investigated the role of zinc supplementation on prevention of diabetic conditions. A double-disease model mimicking hyperlipidemia and type 2 diabetes was created by applying high-fat diet and streptozotocin (STZ) to Wistar rats. We demonstrated that zinc supplementation improved symptoms of diabetes such as polydipsia and increased serum level of high-density lipoprotein cholesterol, indicating that zinc supplementation has a potential beneficial effect on diabetic conditions. The level of maldondialdehyde (MDA), an oxidative stress marker, was reduced in liver by zinc supplementation in high fat-fed rats with or without STZ injection. Meanwhile, we observed an increase in the expression of metallothioneins (MTs) in liver of rats treated with zinc. This suggests that the induction of MTs in liver, which has been shown to be important in scavenging free radicals, could be one of the underlying mechanisms of zinc supplementation on reducing MDA levels in liver. Finally, we found that zinc levels in liver were increased while there was no change in serum zinc levels, indicating that local zinc level might be a critical factor for the induction of MTs. Also, the level of MTs could potentially be an index of zinc bioavailability. Taken together, these results suggest that both zinc and MT could play an important role in balancing nutrition and metabolism to prevent diabetic development.
Journal of physiology and biochemistry 05/2012; · 1.71 Impact Factor
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ABSTRACT: The pigments from Rhodotorula glutinis were separated by using thin layer chromatography, and the result showed that Rhodotorula glutinis cells could synthesize at least three kinds of pigments, which were beta-carotene, torulene, and torularhodin. The Raman spectra based on the three pigments were acquired, and original spectra were preprocessed by background elimination, baseline correction, and three-point-smoothing, then the averaged spectra from different pigments were investigated, and the result indicated that Raman shift which represents C-C bond was different, and the wave number of beta-carotene demonstrated the largest deviation, finally torulene and torularhodin in Rhodotorula glutinis had more content than beta-carotene. Quantitative analysis of Raman peak height ratio revealed that peak height ratio of pigments showed little difference, which could be used as parameters for further research on living cells, providing reference content of pigments. The above results suggest that Raman spectroscopy combined with thin layer chromatography can be applied to analyze pigments from Rhodotorula glutinis, provides abundant information about pigments, and serves as an effective method to study pigments.
Guang pu xue yu guang pu fen xi = Guang pu 03/2012; 32(3):695-8. · 0.84 Impact Factor
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ABSTRACT: To observe the pathological changes in exfoliated cells from lower respiratory tract mucosa in patients undergoing mechanical ventilation for various duration.
The secretion of lower respiratory tract was collected in 144 patients of cerebral vascular diseases undergoing invasive mechanical ventilation, and exfoliated cells were examined under microscope at different time points. According to specimen collection time they were divided into five groups: less than 8 hours (28 cases), 8-24 hours (46 cases), 24 hours-3 days (22 cases), 3-7 days (20 cases), 7-28 days (14 cases), and over 28 days (14 cases). Then the injury to airway mucosa at different time of mechanical ventilation was determined.
The mucosal injury of trachea and bronchial was worsened gradually with the prolongation of mechanical ventilation. Airway mucosa structure of less than 8-hour of mechanical ventilation was generally normal, while most cells after 8-24 hours and 24 hours-3 days of ventilation showed mild injury, and most cells from tracheal and bronchial mucosa showed severe injury after 3-7 days, 7-28 days and over 28 days of ventilation. Except the difference was not statistically significant between 7-28 days and over 28 days group (P>0.05), the differences between remaining groups were statistically significant (all P<0.05). The correlated coefficient between the duration of mechanical ventilation and airway mucosal injury was 0.781 (P<0.05), and the difference was statistically significant.
There are linear correlation between length of mechanical ventilation and the airway mucosal cell injury; injuries emerges when the mechanical ventilation time exceeds 8 hours with the prolongation mechanical ventilation time, there is severe damage of airway mucosa when mechanical ventilation lasted more than 7 days, but there is no difference in extent of injury after 7 days.
Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue 10/2009; 21(10):587-9.
