Amy E S Stone

Publications (4)8.83 Total impact

• Source

  Available from: ufvetmedepidemiology.com

  Article: Education of global veterinarians.

  Jorge A Hernandez, Traci M Krueger, Sheilah A Robertson, Natalie Isaza, Ellis C Greiner, Darryl J Heard, Amy E S Stone, Michelle L Bellville, Victoria Condor-Williams
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  ABSTRACT: In 2003, the University of Florida (UF) College of Veterinary Medicine (CVM) created an Office of International Programs (OIP) in response to one of ten initiatives of the UF Strategic Plan: internationalization of the curriculum. The OIP has developed coursework that provides students with an opportunity for international exposure during the veterinary curriculum at three levels. In Level 1 (on campus) students can participate in a seminar series in global health: www.ufglobalhealth.org. This is an elective course offered to professional students at the UF Health Science Center (Dentistry, Medicine, Pharmacy, Public Health, and Veterinary Medicine). In Level 2 (abroad), students can participate in structured study abroad programs under the supervision of UF faculty and international scholars from collaborative institutions abroad. In Level 3 (on campus and abroad), students can participate in a certificate program in international veterinary medicine. This is a 15-credit program, parallel to the veterinary curriculum. By offering courses on campus and abroad, we want to empower the curriculum with a global perspective of the veterinary profession, as well as with a humanist education that can help students recognize the importance of respect for cultural differences and the reasons for different degrees of development and growth in the world. In addition, this paper presents the need for veterinary medicine and other disciplines in the health sciences to communicate with other disciplines in the social sciences and natural sciences to create development practitioners equipped with cross-disciplinary knowledge and skills needed to formulate, implement and evaluate solutions aimed at breaking the cycle of poverty and disease in low income societies. Finally, this paper makes a call to the American Veterinary Medical Association Council on Education to assess the need to recognize the importance of internationalization of the veterinary curriculum as a key standard for accreditation of colleges or schools of veterinary medicine.
  Preventive Veterinary Medicine 10/2009; 92(4):275-83. · 2.05 Impact Factor
• Article: Expression of a Porphyromonas gingivalis hemagglutinin on the surface of a Salmonella vaccine vector.

  Ryutaro Isoda, Scott P Simanski, Latha Pathangey, Amy E S Stone, Thomas A Brown
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  ABSTRACT: Live, attenuated Salmonella strains can serve as vectors for the delivery of recombinant vaccine antigens for development of oral mucosal vaccines. Various vaccine parameters can affect the immune responses elicited by Salmonella vectors, including the expression level, location and timing of expressed antigens. We have previously established immunogenic Salmonella enterica serovar Typhimurium strains which cytoplasmically express hemagglutinin B (HagB) of Porphyromonas gingivalis, a putative periodontal pathogen. In this study, we sought to determine whether the 39 kDa HagB protein could be stably expressed on the surface of an avirulent Salmonella vaccine strain. The hagB gene was cloned into an expression plasmid as a C-terminal fusion with Lpp-OmpA, a hybrid surface display system. High expression of Lpp-OmpA-HagB proved to be toxic to the vaccine strain, and it was necessary to introduce attenuating mutations in the trc promoter. Stable expression was obtained in transformants with promoter mutations that resulted in low levels of expression. The expression of Lpp-OmpA-HagB was confirmed by ELISA and Western blot. Localization to the outer membrane/periplasm was confirmed by transmission electron microscopy using immunogold labeling, surface labeling of whole mounts using electron microscopy, flow cytometry, and by quantitation of HagB in cytoplasmic, as well as inner and outer cell membrane fractions. When delivered orally in mice, the surface-expressing strain induced higher serum IgG and IgA responses to HagB than a cytoplasmic expressing strain, while responses in secretions were comparable. These results suggest that surface localization may differentially enhance the immunogenicity of antigens expressed by live, avirulent Salmonella vaccine vectors.
  Vaccine 02/2007; 25(1):117-26. · 3.77 Impact Factor
• Article: IL-12 reduces the severity of Sendai virus-induced bronchiolar inflammation and remodeling.

  Amy E S Stone, Steeve Giguere, William L Castleman
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  ABSTRACT: The goal of this research was to determine whether differential pulmonary IL-12 gene expression controls susceptibility to Sendai virus-induced chronic airway inflammation and fibrosis in inbred rat strains. Sendai virus-resistant F344 rats and susceptible BN rats were studied from 1 to 14 days following virus inoculation. F344 rats had 3.4-fold higher IL-12 mRNA levels detected by real-time PCR in lung than BN rats as early as two days following inoculation. This increase in mRNA was associated at two days with increased total IL-12 protein and with a 2-fold increase in numbers of bronchiolar, OX-6-positive dendritic cells and an increased number of IL-12 p40-positive, bronchiolar macrophages and dendritic cells (p<0.05). Virus-susceptible BN rats treated with 3 mug of recombinant, mouse IL-12 intraperitoneally at the time of virus inoculation had a 22.1% decrease in severity of chronic bronchiolar inflammation and a 23.8% decrease in fibrosis compared to virus-inoculated BN rats treated with saline. IL-12 treatment induced increased IFN-gamma mRNA and protein expression after virus inoculation (p<0.05). The results demonstrate that there is differential pulmonary IL-12 gene expression between virus-susceptible and resistant rat strains and that IL-12 treatment can provide significant protection from virus-induced chronic airway inflammation and remodeling during early life.
  Cytokine 11/2003; 24(3):103-13. · 3.02 Impact Factor
• Article: Expression of a Porphyromonas gingivalis hemagglutinin on the surface of a Salmonella vaccine vector

  Ryutaro Isoda, Scott P. Simanski, Latha Pathangey, Amy E.S. Stone, Thomas A. Brown
  [show abstract] [hide abstract]
  ABSTRACT: Live, attenuated Salmonella strains can serve as vectors for the delivery of recombinant vaccine antigens for development of oral mucosal vaccines. Various vaccine parameters can affect the immune responses elicited by Salmonella vectors, including the expression level, location and timing of expressed antigens. We have previously established immunogenic Salmonella enterica serovar Typhimurium strains which cytoplasmically express hemagglutinin B (HagB) of Porphyromonas gingivalis, a putative periodontal pathogen. In this study, we sought to determine whether the 39 kDa HagB protein could be stably expressed on the surface of an avirulent Salmonella vaccine strain. The hagB gene was cloned into an expression plasmid as a C-terminal fusion with Lpp-OmpA, a hybrid surface display system. High expression of Lpp-OmpA-HagB proved to be toxic to the vaccine strain, and it was necessary to introduce attenuating mutations in the trc promoter. Stable expression was obtained in transformants with promoter mutations that resulted in low levels of expression. The expression of Lpp-OmpA-HagB was confirmed by ELISA and Western blot. Localization to the outer membrane/periplasm was confirmed by transmission electron microscopy using immunogold labeling, surface labeling of whole mounts using electron microscopy, flow cytometry, and by quantitation of HagB in cytoplasmic, as well as inner and outer cell membrane fractions. When delivered orally in mice, the surface-expressing strain induced higher serum IgG and IgA responses to HagB than a cytoplasmic expressing strain, while responses in secretions were comparable. These results suggest that surface localization may differentially enhance the immunogenicity of antigens expressed by live, avirulent Salmonella vaccine vectors.
  Vaccine.