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Publications (2)1.31 Total impact

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    ABSTRACT: Benign bile duct stricture is common in surgical practice. The complications of late stricture formation and biliary sepsis still occur in bile duct reconstruction. Many biological and artificial materials have been used to replace the damaged bile duct to avoid bilio-enteric anastomosis, which bypasses the biliary sphincter mechanism. Amniotic epithelial (AE) cells are known to have unique characteristics, such as low-level expression of major histocompatibility complex antigens and a less restricted differentiation potential. AE cells differentiate into different cell types from all three germ layers, including cardiomyocytic, myocytic, osteocytic, adipocytic (mesodermal), pancreatic, hepatic (endodermal), neural, and astrocytic (neuroectodermal) cells in vitro, suggesting a promising candidate to reconstruct the damaged bile duct. Human amniotic grafts (as a source of stem cells) with or without vascularized peritoneomuscular flap were used to repair damaged bile ducts (non-circumferential and circumferential bile duct loss) in a dog model. Non-circumferential bile duct loss appeared to be satisfactorily repaired using amnion graft alone. However, circumferential duct loss was not adequately repaired with amnion graft alone, but it was adequately repaired using amnion graft with a vascularized peritoneomuscular flap. In adequately repaired cases, histological examination demonstrated that the biliary mucosal endothelium had grown over the amniotic membrane graft. Collectively, the data presented here suggest that the use of human amnion as a source of amniotic stem cells provides a very promising tool for tissue reconstruction.
    Medical science monitor: international medical journal of experimental and clinical research 10/2009; 15(9):BR243-7. · 1.22 Impact Factor
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    ABSTRACT: Amniotic membrane is considered a promising procedure as a graft in the field of ophthalmology and skin reconstruction. It has been shown to decrease inflammation, fibrosis, elicit no host immune reaction and also has antibiotic actions. Recently, the amnion has been shown to contain cells capable of plasticity. Therefore, we felt its application could be extended to include surgical reconstruction. We used ureteric reconstruction to study the feasibility of the amnion as a graft in these situations. We induced different degrees of injury in the ureter of dogs and reconstructed the defect with amniotic membrane. In addition, we also studied the effects of supplying the graft with a source of blood supply. The subjects were sacrificed after 6 weeks and histopathological examination showed impressive repair of the defect. The post reconstruction complications seen with present grafts were not seen with amniotic membrane. Therefore, amniotic membrane has produced noteworthy results and its potential should be further evaluated in order to be used as a cheap, readily available source of graft for the various surgical reconstruction procedures.
    Indian Journal of Surgery 06/2009; 71(3):121-7. · 0.09 Impact Factor