Zongsuo Liang

Zhejiang University of Science and Technology, Hang-hsien, Zhejiang Sheng, China

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Publications (54)109.02 Total impact

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    ABSTRACT: Four Panax genus species (Panax notoginseng (PN), Panax stipuleanatus (PS), Panax vietnamensis (PV) and Panax japonicus (PJ)) are all distributed in Southeast Asia. Besides PN, the other three species attracted little attention because of their limited distribution area, less wide application or relatively low quantity. PN had been cultivated as an industrial crop for hundreds years in China while the other three species as substitutes for PN in minority ethnic groups had no large-scale cultivation until now. The saponins of the four medical plants had been investigated in previous study while there has been virtually no reports describing their other compounds. In this study, the gas chromatography-mass spectrometry (GC–MS) was used to compare the volatile oils compounds of the four species. A total of 146 compounds were identified among all the species and every species contained compounds with similar quantity (all about 70). The falcarinol (28.86%) and andrographolide (38.35%) were the main compositions for PN. Furthermore, falcarinol was the dominant composition for PV (70.65%) and PS (64.61%), while pentadecanoic acid and ledene oxide-(II) were the high content compounds for PJ, accounting 9.31% and 8.39%, respectively. In addition, hierarchical clustering analysis was conducted to evaluate the relationship between the four Panax species based on contents (%) of chemical constituents in volatile oils. The results indicated that the PV and PS exhibited high similarity of volatile oils compounds. The PN, PV and PS could be used as the raw materials of falcarinol presenting well biological activities.
    Industrial Crops and Products 11/2015; 74. DOI:10.1016/j.indcrop.2015.05.059 · 2.84 Impact Factor
  • Bo Li · Chenlu Zhang · Liang Peng · Zongsuo Liang · Xijun Yan · Yonghong Zhu · Yan Liu
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    ABSTRACT: Salvia is an important genus containing nearly 1000 species of the Labiatae family; most species of Salvia are traditional herbal medicines and industrial materials with various active components. Salvia is also evidently rich in essential oils and phenolic acids. This study aimed to investigate the chemical composition of the essential oils and the content of phenolic acids in four Salvia species cultivated in Yangling, PR China. The essential oils from the fresh leaves and flowers of Salvia miltiorrhiza, Salvia przewalskii, Salvia officinalis, and Salvia deserta were obtained by simultaneous distillation extraction and evaluated by GC–MS; the phenolic acids were analyzed by HPLC. Essential oil derived from S. deserta flowers contained at most 72 compounds, while 11 compounds were common to all Salvia oils. Aromadendrene oxide-(1) (8.3%) and β-caryophyllene (11.05%) were the major constituents in essential oils from S. miltiorrhiza leaves and flowers, respectively. The limonene content in leaf oil markedly differed from that of flower oil from S. przewalskii; thujone was the predominant component in both the leaf and flower oils of S. officinalis. Moreover, β-phellandrene (29.74%) was the most abundant compound in the essential oil of S. deserta flowers. Composition of essential oils from these Salvia species showed strong tissue and organ specificity. Furthermore, the contents of ferulic acid, caffeic acid and rosmarinic acid in the aerial parts of these species were also investigated. The leaves of S. przewalskii and flowers of S. officinalis were rich in rosmarinic acid, with contents of 64.1 ± 3.9 and 53.4 ± 2.9 mg/g dry weight, respectively. Thus, S. przewalskii leaves could be considered a good source of rosmarinic acid.
    Industrial Crops and Products 07/2015; 69. DOI:10.1016/j.indcrop.2015.02.047 · 2.84 Impact Factor
  • Molecules 05/2015; 20(5):7574-7585. DOI:10.3390/molecules20057574 · 2.42 Impact Factor
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    ABSTRACT: Blind and excessive application of fertilizer was found during the cultivation of Panax notoginseng in field, as well as the increase of root rot disease incidence.
    Journal of ginseng research 04/2015; 6. DOI:10.1016/j.jgr.2015.04.003 · 2.30 Impact Factor
  • Yonghong Liu · Wenting Liu · Zongsuo Liang
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    ABSTRACT: Pinellia ternata (Thunb.) Berit., a perennial herb belonging to Araceae, is one of the few medicinal plants to produce purine alkaloids. It is speculated that endophytic bacteria from P. ternata may produce guanosine or inosine. However, there is no report about endophytic bacteria in P. ternata. In this study, endophytic bacteria were isolated from P. ternata and examined for the first time. This study finds a novel way to increase the yield of P. ternata herb, and to provide some new alkaloid producers. Plant material includes leaves, tubers, and roots of cultivated and wild P. ternata. The dilutions were smeared onto beef extract-peptone medium and cultured at 28 °C in darkness for 48-72 h. Co-culture treatments were prepared by inoculating 100 mL liquid 1/2 MS medium with bacterial culture broth at concentrations of 0 (control), 0.5%, and 1.5% (v/v). Of the 34 endophytic bacterial colonies isolated from P. ternata leaves, roots, and tubers, five strains were able to produce purine alkaloids. Results from 16s rDNA sequence analysis indicated that the bacteria belonged to Bacillus cereus, Aranicola proteolyticus, Serratia liquefaciens, Bacillus thuringiensis, and Bacillus licheniformis. Co-culture with living Serratia liquefaciens cells increased PLB growth by 58-71%. Co-culture with living Bacillus licheniformis cells increased PLB growth by 4-11%. This study provides a novel way for improving the yield of P. ternata herb, and for the production of purine alkaloids by the fermentation industry.
    Pharmaceutical Biology 04/2015; 53(10):1-4. DOI:10.3109/13880209.2015.1016580 · 1.34 Impact Factor
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    ABSTRACT: Magnesium (Mg) is one of the most important nutrients, involves mainly in plant growth and development. However, the signaling pathways response to magnesium stresses (MgSs) is known little, but several studies lately may improve the research development. Several phytohormones such as abscisic acid (ABA), ethylene, auxins, and their factors were found responding to MgSs, and there may be some signal pathways linking these hormones and downstream reactions together, e.g., carbon fixation and transfer, starch and sugar metabolism, ion uptaking and reactive oxygen species (ROS) increasing. Consequently, Arabidopsis morphogenesis is remodeled. In this review, we mainly discussed recent literatures involving in plant response to MgSs (Mg deficiency (MgD) and Mg toxicity (MgT)), including plant morphogenesis remodeling, magnesium transporters and signaling transductions. Moreover, the future study challenges related to the responding signalings to MgSs in plants are also presented. Regardless, the iceberg of signal transduction of MgSs in plants is appeared.
    Plant signaling & behavior 03/2015; 10(3). DOI:10.4161/15592324.2014.992287
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    ABSTRACT: Salvia castanea Diels f. tomentosa Stib. is an endemic medicinal plant distributed in China, and the notably high content of tanshinone IIA in the root is proven effective for the therapy of heart diseases. Hairy root induction of this Salvia species was inoculated with Agrobacterium rhizogenes strain ATCC 15834. Transformed hairy root was cultured in 6,7-V liquid medium for growth kinetics assessment and elicitation. An S curve was present in the hairy root cultures based on the fresh and dry weights with an interval of 3 days. An optimum concentration of the applied elicitors (15 μM Ag(+), 200 μM methyl jasmonate, and 200 mg l(-1) yeast extract elicitor) benefitted both the growth status and tanshinone accumulation in the hairy root cultures. Tanshinone IIA contents were mostly stimulated 1.8-fold and 1.99-fold compared with the control by Ag(+) and methyl jasmonate elicitation, respectively. Yeast extract dramatically enhanced dry mass accumulation, while it promoted cryptotanshinone content of 2.84 ± 0.33 mg g(-1) dry weight at most in the hairy root cultures. Selected elicitors diversely influenced tanshinone accumulation in the time courses of hairy root cultures within 7 days. Furthermore, transcripts of selected genes in the tanshinone biosynthetic pathway were remarkably upregulated with elicitation. Yeast extract elicitor heightened 13.9-fold of isopentenyl diphosphate isomerase expression level at 12 h, while it increased 16.7-fold of geranylgeranyl diphosphate synthase transcript at 24 h compared with that of the control, which was more effective than Ag(+) and methyl jasmonate. This study provided a convenient hairy root culture system of S. castanea Diels f. tomentosa Stib. for tanshinone production for the first time.
    Protoplasma 03/2015; DOI:10.1007/s00709-015-0790-9 · 3.17 Impact Factor
  • Xuhong Du · Yulong Zhao · Dongfeng Yang · Yan Liu · Kai Fan · Zongsuo Liang · Ruilian Han
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    ABSTRACT: Panax notoginseng (Burk.) F.H. Chen (commonly known as Tianqi or Sanqi) is a famous traditional Chinese medicine and has been widely used for treatment in cardio- and cerebro-vascular diseases. However, the quality assessment of Sanqi is quite difficult because of ingredients complexity. In this work, the chemical fingerprints of 15 batches of P. notoginseng from different sources were investigated by ultra-performance liquid chromatography (UPLC). Fourteen common peaks in these samples were detected including 20(S)-protopanaxatriol saponins (PTS) and 20(S)-protopanaxadiol saponins (PDS). These samples were divided into three clusters by hierarchical clustering analysis (HCA). Cluster I and III possessed stronger anti-coagulation effects than cluster II. Multiple linear regression analysis (MLRA) showed that notoginsenoside R1, ginsenoside Rb1, ginsenoside Rd and an unknown compound might be the major effective compoounds for the quality control of Sanqi. PDS (Rb1, Rd, and the unknown compound) possesses a stronger anticoagulant activity than PTS (R1 only). This conjecture was confirmed by the dose–effect relationship evaluation of PDS and PTS. This study provides a general correlation model of fingerprint and efficacy relationship for the quality control of Sanqi and other TCMs.
    Analytical methods 02/2015; 7(7). DOI:10.1039/C4AY02277G · 1.94 Impact Factor
  • Pengda Ma · Jingying Liu · Anne Osbourn · Juane Dong · Zongsuo Liang
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    ABSTRACT: Salvia miltiorrhiza (Chinese name: dānshēn) is used in traditional Chinese medicine for the treatment of cardiovascular and cerebrovascular diseases. The tanshinones represent the most important biological active class of compounds present in dānshēn extracts. They are synthesized via either the cytoplasmic mevalonate or the plastidial 2-C-methyl-D-erythritol-4-phosphate pathway. Here, we summarize recent discoveries regarding the mechanisms underlying tanshinone biosynthesis and how the process is regulated. Tanshinone accumulation in planta is affected by a range of elicitors and by the composition of the culture medium. Its production in hairy root cultures can be enhanced by the over-expression of genes encoding 1-deoxy-D-xylulose 5-phosphate synthase, 3-hydroxy-3-methylglutaryl-CoA reductase, geranylgeranyl diphosphate synthase and allene oxide cyclase. The pathway leading to the biosynthesis of the tanshinone precursors miltiradiene and ferruginol, has been engineered in yeast.
    RSC Advances 02/2015; 5(23). DOI:10.1039/C4RA13459A · 3.84 Impact Factor
  • Dongfeng Yang · Xuhong Du · Zongqi Yang · Zongsuo Liang · Zhixin Guo · Yan Liu
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    ABSTRACT: Plant secondary metabolites play an important role in the fields of food, medicine, agriculture and biofuels. Secondary metabolites are an important focus of crop breeding and metabolic engineering research. However, our understanding of secondary metabolism is far from complete, particularly in non-model plants. Functional genomics, which includes transcriptomics, proteomics, and metabolomics, opens a new avenue for deciphering secondary metabolism. Here we review the applications of functional genomics in secondary metabolism, including the discovery of novel genes, the identification of gene function, and the detection of novel pathways of the metabolic network. Results from these studies will accelerate our understanding of elicitation mechanism of secondary metabolism and how plants interact with their environment, ultimately improving the production of secondary metabolites by means of metabolic engineering. This article is protected by copyright. All rights reserved
    Engineering in Life Sciences 09/2014; 14(5). DOI:10.1002/elsc.201300075 · 1.89 Impact Factor
  • Liang Peng · Mei Ru · Kunru Wang · Bo Li · Yancui Wang · Pengguo Xia · Zongsuo Liang
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    ABSTRACT: Salvia miltiorrhiza is a significant source of bioactive compounds providing human health effects. Here, we surveyed root yield and the active constituents' divergences of second generation S. miltiorrhiza (SP2) responding to a spaceflight environment. High-performance liquid chromatography was conducted for the comprehensive constituents' characterizations of 28 SP2 lines (224 individuals) and the ground control (eight individuals). The results showed that the mean fresh and dry weight of roots ranged from 116 to 172 g and 25 to 119 g, respectively, in SP2 lines. In addition, the mean contents of four tanshinone compounds (tanshinone I, tanshinone IIA, cryptotanshinone, and dihydrotanshinone I) of 28 SP2 lines varied from 0.32 to 1.04 mg · g(-1), 0.47 to 2.39 mg · g(-1), 0.25 to 1.60 mg · g(-1), and 0.53 to 1.67 mg · g(-1), respectively. Except for salvianolic acid B, which varied drastically from 72 % to 201 % of the ground control treatment, the other six phenolic acid contents of the 28 SP2 lines all increased after spaceflight. Principal component analysis was performed to obtain an overview of the distribution of all samples, and score plots clearly separated the SP2 accessions from ground controls. Moreover, a positive relationship was observed between tanshinone I and tanshinone IIA (r = 0.790, p < 0.01), and rosmarinic acid was positively correlated with salvianolic acid B (r = 0.728, p < 0.01). In conclusion, this study demonstrated that a spaceflight environment induced SP2 accessions remarkably in the variation of root yield and active constituent content.
    Planta Medica 08/2014; 80(12). DOI:10.1055/s-0034-1382908 · 2.34 Impact Factor
  • Liang Peng · Mei Ru · Bangqing Wang · Yong Wang · Bo Li · Jing Yu · Zongsuo Liang
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    ABSTRACT: Salvia miltiorrhiza is one of the most important traditional Chinese medicinal plants for its therapeutic effects. In the present study, morphological traits, ISSR (inter-simple sequence related) and SRAP (sequence-related amplified polymorphism) markers were used to analyze the genetic diversity of 59 S. miltiorrhiza phenotypes. Out of the 100 ISSR primers and 100 SRAP primer combinations screened, 13 ISSRs and 7 SRAPs were exploited to evaluate the level of polymorphism and discriminating capacity. The results showed that the 13 ISSRs generated 190 repeatable amplified bands, of which 177 (93.2%) were polymorphic, with an average of 13.6 polymorphic fragments per primer. The 7 SRAPs produced 286 repeatable amplified bands, of which 266 (93.4%) were polymorphic, with an average of 38.1 polymorphic fragments per primer. Cluster analysis readily separated different morphological accessions, wild and cultivated controls based on morphological traits, ISSR and SRAP markers. The study indicated that morphological traits, ISSR and SRAP markers were reliable and effective for assessing the genetic diversity of phenotypic S. miltiorrhiza accessions. The overall results suggested that the introduction of genetic variation from morphology-based germplasms enlarged the genetic base for the collection, conservation and further breeding program of S. miltiorrhiza germplasm.
    Biochemical Systematics and Ecology 08/2014; 55:84–92. DOI:10.1016/j.bse.2014.01.020 · 1.17 Impact Factor
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    ABSTRACT: Little information is available about signaling response to magnesium toxicity (MgT) in plants. This study presents the first evidence that abscisic acid (ABA) and DELLA proteins participate in signaling response to long-term MgT in Arabidopsis thaliana (Landsberg erecta). Morphological, physiological, and molecular characteristics of a wild-type and two Arabidopsis mutants, ABA-insensitive mutant abi1-1 and constitutive elevated GA response mutant quadruple-DELLA (DELLA-Q: gai-t6 rga-t2 rgl1-1 rgl2-1) were monitored under MgT and normal magnesium conditions. Two weeks of MgT treatment strongly influenced the growth of young plants, but growth inhibition of the DELLA-Q and abi1-1 mutants was less than that of the wild-type plants. Exogenous ABA further inhibited the growth of the DELLA-Q mutants, similar to that of the wild-type. Both ABA and MgT also promoted DELLA protein RGA accumulation in the nuclei. Transcriptional analysis supported these results and revealed that a complex signaling network has responded to MgT in Arabidopsis. DELLA enhancement, which depends on ABI1, contributed to the remodeling growth and development of young seedlings.
    Plant and Cell Physiology 07/2014; 55(10). DOI:10.1093/pcp/pcu102 · 4.98 Impact Factor
  • Yan Yan · Shuncang Zhang · Jiayi Zhang · Pengda Ma · Jiali Duan · Zongsuo Liang
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    ABSTRACT: Our study found that except Novosphingobium resinovorum (B5) Salvia miltiorrhiza root endophytic bacteria Pseudomonas brassicacearum sub sp. neoaurantiaca (B1), Rhizobium radiobacter (B2), Pseudomonas thivervalensis (B3), Pseudomonas frederiksbergensis (B4) significantly improved the activity of key enzymes 3-hydroxy-3-methyglutary1-CoA reductase and 1-deoxy-d-xylulose-5-phosphate synthase in the biosynthetic pathway of tanshinones. Specifically, HMGR activity with B1 treatment increased 2.1-fold that of control, 1-deoxy-d-xylulose-5-phosphate synthase activity with B2 treatment increased 5.0-fold that of control, which caused a significant increase in tanshinone content in the hairy roots. The dihydrotanshinone I and cryptotanshinone content under B1 treatment increased 19.2-fold and 11.3-fold, respectively, and total tanshinone content increased 3.7-fold that of control. The five endophytic bacteria B1, B2, B3, B4 and B5 all significantly decreased phenylalanine ammonia-lyase and tyrosine aminotransferase activity in hairy roots, of which, B3 treatment decreased phenylalanine ammonia-lyase activity by 46.2 %, and B2 treatment decreased tyrosine aminotransferase activity by 44.7 % compared with the control. Each of the five endophytic bacteria decomposed rosmarinic acid and salvianolic acid B, which caused a significant decrease in rosmarinic acid and salvianolic acid B content in hairy roots, with B2 treatment decreasing rosmarinic acid and salvianolic acid B content by 94.5 and 89.0 %, respectively, compared with the control. The five endophytic bacteria also inhibited the growth of S. miltiorrhiza hairy roots, of which, B2 and B4 treatment decreased hairy root biomass by 55.2 and 51.3 %, respectively, compared with the control, while hairy roots promoted the growth of B4 and B5 and inhibited the growth of B1 and B3.
    Acta Physiologiae Plantarum 05/2014; 36(5). DOI:10.1007/s11738-014-1484-1 · 1.52 Impact Factor
  • Yan Yan · Shuncang Zhang · Dongfeng Yang · Jiayi Zhang · Zongsuo Liang
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    ABSTRACT: The fermentation broth and mycelium pellet of Streptomyces pactum Act12 (Act12) may promote the accumulation of soluble sugar when added to Salvia miltiorrhiza hairy roots, increasing the accumulation level by as much as 23.20 % compared with the control; it may also inhibit the accumulation of soluble protein in the hairy roots, decreasing it by as much as 17.96 % compared with the control. The ACT12 also has a certain promotional effect on the growth of hairy root at an appropriate concentration of elicitors and upregulates the expression of genes 3-hydroxy-3-methyglutary1-CoA reductase (HMGR), 1-deoxy-D-xylulose 5-phosphate synthase (DXS), 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR), and geranylgeranyl diphosphate synthase (GGPPS). Among these effects, that of the HMGR gene expression is as high as 33.66 times that of the control, indicating that the test Streptomyces pactum may efficiently adjust the secondary metabolism of S. miltiorrhiza at the level of gene transcription, thereby greatly increasing the accumulation level of tanshinone in the hairy roots; among which, the cryptotanshinone levels increased most significantly, as much as 33.63 times that of the control, and the total tanshinone levels were 12.61 times that of the control.
    Applied biochemistry and biotechnology 04/2014; 173(4). DOI:10.1007/s12010-014-0876-4 · 1.74 Impact Factor
  • Pengguo Xia · Hongbo Guo · Zongsuo Liang · Xiuming Cui · Yan Liu · Fenghua Liu
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    ABSTRACT: Sanchi (Panax notoginseng) root has been widely used as traditional herb to stanch blood, disperse gore and reduce pain in China for centuries. However, the nutritional information about its seed was unknown. Sanchi seeds obtained from Yunnan Province were analyzed for their nutritional composition, fatty acid, amino acid profile and sugar contents. Sanchi seeds were abundant in fats (46.35 %) and proteins (23.90 %). Notably, seven fatty acid compositions were determined and abundant in unsaturated fatty acid (99.56 %), containing 95.71 % oleic acid. Sanchi seed proteins were rich in glutenin, globulin and albumin (28.63, 27.83 and 26.81 %, respectively). Sanchi seed contain 17 kinds of amino acids, of which nine were essential amino acids, accounting for 41.30 %. These nutritional compositions indicate that Sanchi seed has the potentiality to be exploited as human edible oil, industrial use, new medicine or healthcare products for diabetes patients due to low sugar content.
    Genetic Resources and Crop Evolution 03/2014; 61(3). DOI:10.1007/s10722-014-0082-9 · 1.48 Impact Factor
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    ABSTRACT: Phenolic acids and tanshinones are two groups of bioactive ingredients in Salvia miltiorrhiza Bunge. As a heavy metal elicitor, it has been reported that Ag+ can induce accumulations of both phenolic acids and tanshinones in S. miltiorrhiza hairy roots. In this study, the effects of Ag+ treatment on accumulations of six phenolic acids and four tanshinones in S. miltiorrhiza hairy roots were investigated. To further elucidate the molecular mechanism, expressions of key genes involved in the biosynthesis of these ingredients were also detected. The results showed that although the total phenolic acids content was almost not affected by Ag+, accumulations of rosmarinic acid (RA), caffeic acid and ferulic acid were significantly increased, while accumulations of salvianolic acid B (LAB), danshensu (DSU) and cinnamic acid were decreased. We speculate that LAB probably derived from the branch pathway of DSU biosynthesis. Contents of four tanshinones were enhanced by Ag+ and their accumulations were more sensitive to Ag+ than phenolic acids. Genes in the upstream biosynthetic pathways of these ingredients responded to Ag+ earlier than those in the downstream biosynthetic pathways. Ag+ probably induced the whole pathways, upregulated gene expressions from the upstream pathways to the downstream pathways, and finally resulted in the enhancement of ingredient production. Compared with phenolic acids, tanshinone production was more sensitive to Ag+ treatments. This study will help us understand how secondary metabolism in S. miltiorrhiza responds to elicitors and provide a reference for the improvement of the production of targeted compounds in the near future.
    Molecules 01/2014; 20(1):309-24. DOI:10.3390/molecules20010309 · 2.42 Impact Factor
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    ABSTRACT: Rosmarinic acid and salvianolic acid B are two important phenolic compounds with therapeutic properties in Salvia miltiorrhiza Bunge. The biosynthesis of rosmarinic acid is initiated by two parallel pathways, namely the phenylpropanoid pathway and the tyrosine-derived pathway. Salvianolic acid B is a structural dimer of rosmarinic acid and is believed to be derived from rosmarinic acid. In the current study, methyl jasmonate (MeJA) and hyphal extracts from fungi were used as elicitors to examine the relationship between enzymes in the two parallel pathways and accumulation of phenolic compounds in S. miltiorrhiza hairy root cultures. The results showed that accumulations of rosmarinic acid, salvianolic acid B and total phenolics were enhanced by MeJA while suppressed by fugal extracts. Responses of enzymes in the tyrosine-derived pathway, at both the gene transcript and enzyme activity levels, showed a better consistency with alterations of phenolic compounds content after the two elicitors treated. Our study implied that compared with enzymes in the phenylpropanoid pathway, enzymes in the tyrosine-derived pathway are more correlated to rosmarinic acid and salvianolic acid B biosynthesis in S. miltiorrhiza hairy roots.
    Journal of Bioscience and Bioengineering 11/2013; DOI:10.1016/j.jbiosc.2013.10.013 · 1.79 Impact Factor
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    ABSTRACT: Salvia miltiorrhiza Bunge is one of the most renowned traditional medicinal plants in China. Phenolic acids that are derived from the rosmarinic acid pathway, such as rosmarinic acid and salvianolic acid B, are important bioactive components in S. miltiorrhiza. Accumulations of these compounds have been reported to be induced by various elicitors, while little is known about transcription factors that function in their biosynthetic pathways. We cloned a subgroup 4 R2R3 MYB transcription factor gene (SmMYB39) from S. miltiorrhiza and characterized its roles through overexpression and RNAi-mediated silencing. As the results showed, the content of 4-coumaric acid, rosmarinic acid, salvianolic acid B, salvianolic acid A and total phenolics was dramatically decreased in SmMYB39-overexpressing S. miltiorrhiza lines while being enhanced by folds in SmMYB39-RNAi lines. Quantitative real-time PCR and enzyme activities analyses showed that SmMYB39 negatively regulated transcripts and enzyme activities of 4-hydroxylase (C4H) and tyrosine aminotransferase (TAT). These data suggest that SmMYB39 is involved in regulation of rosmarinic acid pathway and acts as a repressor through suppressing transcripts of key enzyme genes.
    PLoS ONE 09/2013; 8(9):e73259. DOI:10.1371/journal.pone.0073259 · 3.23 Impact Factor
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    Zongsuo Liang · Yini Ma · Tao Xu · Beimi Cui · Yan Liu · Zhixin Guo · Dongfeng Yang
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    ABSTRACT: Salvia miltiorrhiza is one of the most important traditional Chinese medicinal plants because of its excellent performance in treating coronary heart disease. Phenolic acids mainly including caffeic acid, rosmarinic acid and salvianolic acid B are a group of active ingredients in S. miltiorrhiza. Abscisic acid (ABA), gibberellin (GA) and ethylene are three important phytohormones. In this study, effects of the three phytohormones and their interactions on phenolic production in S. miltiorrhiza hairy roots were investigated. The results showed that ABA, GA and ethylene were all effective to induce production of phenolic acids and increase activities of PAL and TAT in S. miltiorrhiza hairy roots. Effects of phytohormones were reversed by their biosynthetic inhibitors. Antagonistic actions between the three phytohormones played important roles in the biosynthesis of phenolic acids. GA signaling is necessary for ABA and ethylene-induced phenolic production. Yet, ABA and ethylene signaling is probably not necessary for GA3-induced phenolic production. The complex interactions of phytohormones help us reveal regulation mechanism of secondary metabolism and scale-up production of active ingredients in plants.
    PLoS ONE 09/2013; 8(9):e72806. DOI:10.1371/journal.pone.0072806 · 3.23 Impact Factor

Publication Stats

313 Citations
109.02 Total Impact Points


  • 2012–2015
    • Zhejiang University of Science and Technology
      Hang-hsien, Zhejiang Sheng, China
  • 2014
    • Northwest University
      Northwest Harborcreek, Pennsylvania, United States
    • Zhejiang Sci-Tech University
      Ch’u-chou, Zhejiang Sheng, China
  • 2009–2014
    • Northwest A & F University
      • College of Life Sciences
      Yang-ling-chen, Shaanxi, China
    • Northwest University
      • College of Life Sciences
      Xi’an, Shaanxi Sheng, China
  • 2013
    • Chinese Academy of Sciences
      Peping, Beijing, China
    • Hangzhou University
      Hang-hsien, Zhejiang Sheng, China