Baoling Yang

Dalian Nationalities University, Lü-ta-shih, Liaoning, China

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Publications (5)15.1 Total impact

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    ABSTRACT: A novel series of carbamate-linked cationic lipids containing hydroxyl headgroup were synthesized and included in formulations for transfection assays. The DNA-lipid complexes were characterized for their ability to bind DNA, their size, ζ-potential and cytotoxicity. Compared with our previously reported cationic transfection lipid DDCDMA lacking the hydroxyl group and the commercially available, these cationic liposomes exhibited relatively higher transfection efficiency.
    Colloids and surfaces B: Biointerfaces 09/2013; 112. DOI:10.1016/j.colsurfb.2013.08.040 · 4.15 Impact Factor
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    ABSTRACT: Two new types of stable ternary complexes were formed by mixing chitosan with DOTAP/pDNA lipoplex and DOTAP with chitosan/pDNA polyplex via non-covalent conjugation for the efficient delivery of plasmid DNA. They were characterized by atomic force microscopy, gel retarding, and dynamic light scattering. The DOTAP/CTS/pDNA complexes were in compacted spheroids and irregular lump of larger aggregates in structure, while the short rod- and toroid-like and donut shapes were found in CTS/DOTAP/pDNA complexes. The transfection efficiency of the lipopolyplexes showed higher GFP gene expression than DOTAP/pDNA and CTS/pDNA controls in Hep-2 and Hela cells, and luciferase gene expression 2-3-fold than DOTAP/pDNA control and 70-120-fold than CTS/pDNA control in Hep-2 cells. The intracellular trafficking was examined by confocal laser scanning microscopy. Rapid pDNA delivery to the nucleus enchanced by chitosan was achieved after 4 h transfection.
    Biotechnology Letters 01/2012; 34(1):19-28. DOI:10.1007/s10529-011-0748-8 · 1.59 Impact Factor
  • Defu Zhi · Shubiao Zhang · Bing Wang · Yinan Zhao · Baoling Yang · Shijun Yu
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    ABSTRACT: The structure of cationic lipids is a major factor for their transfection activity. A cationic lipid generally contains four functional domains: a hydrophilic headgroup, a linker, a backbone domain, and a hydrophobic domain. The structure of the hydrophobic domain determines the phase transition temperature and the fluidity of the bilayer and influences the stability of liposomes, the DNA protection from nucleases, the endosomal escape, the DNA release from complex, and the nuclear penetration. Also, toxicity of the lipids is influenced by the hydrophobic domain. The compounds used for gene delivery are classified according to the structure of the hydrophobic domain as follows: aliphatic chains, steroid domain, and fluorinated domain. In this review, we summarized recent research results concerning the structures of the hydrophobic domain, in order to find the effect of the hydrophobic domain on transfection efficiency. Understanding these would be very important for scientists to prepare novel cationic lipids and design novel formulations with high transfection efficiency.
    Bioconjugate Chemistry 04/2010; 21(4):563-77. DOI:10.1021/bc900393r · 4.51 Impact Factor
  • Yan Duan · Shubiao Zhang · Bing Wang · Baoling Yang · Defu Zhi
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    ABSTRACT: Cationic lipid/DNA complexes (lipoplexes) represent an attractive alternative to viral vectors for cell transfection in vitro and in vivo but still suffer from relatively low efficiency. Comprehension of the interactions between vectors and DNA as well as cellular pathways and mechanisms in DNA entry into cells and ultimately nuclei will lead to the design of better adapted non-viral vectors for gene therapy applications. Here, some recent developments in the field on the pathways and mechanisms involved in lipoplex-mediated transfection are discussed. The techniques that are widely used to study the mechanism of gene delivery are also discussed.
    Expert Opinion on Drug Delivery 09/2009; 6(12):1351-61. DOI:10.1517/17425240903287153 · 4.84 Impact Factor
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    ABSTRACT: Chlorella sp. isolated from municipal wastewater and entrapped in calcium alginate as algal sheets was employed to remove inorganic nutrients from domestic secondary effluents in parallel-plate bioreactor after starvation. The key factors affecting the nutrient removal efficiency, system stability and reuse efficiency of screens were discussed. The results show that cell density and starvation time significantly affected the nutrient uptake. A complete removal of NH 4 + -N and PO 4 3--P was achieved within 4 h of treatment with the optimal cell density in the mixture of algal and 3 mm gel sheets after third cycle. Six cycles of wastewater treatment were accomplished in 18 days, achieving higher removal efficiency. NH 4 + -N removal efficiency was 81% after 1.25 h and 98.81% after 4 h, while PO 4 3--P removal efficiency was 77.4% after 1.25 h and 100% after 4 h. It was confirmed that the immobilized Chlorella sp. has great potentialities in nutrient removal.