C Cortese

Sant'Andrea Medical Hospital, Spezia, Liguria, Italy

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Publications (123)302.6 Total impact

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    ABSTRACT: p63 is an important regulator of epithelial development expressed in different variants, containing (TA) or lacking (ΔN) the N-terminal transactivation domain. The different isoforms regulate stem cell renewal and differentiation as well as cell senescence. Several studies indicate that p63 isoforms also play a role in cancer development, however very little is known on the role played by p63 in regulating the Cancer Stem phenotype. Here we investigate the cellular signals regulated by TAp63 andΔNp63 in a model of epithelial-cancer stem cells. To this end we used colon cancer stem cells, over-expressing either TAp63 or ΔNp63 isoforms, to carry out a proteomic study by chemical labeling approach coupled to network analysis. Our results indicate that p63 is implicated in a wide range of biological processes, including metabolism. This was further investigated by a targeted strategy, both at protein and metabolite level. The overall data show that TAp63 over-expressing cells are more glycolytic active than ΔNp63 cells, indicating that the two isoforms may regulate the key steps of glycolysis in an opposite manner. The mass spectrometry proteomics data of the study have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with dataset identifiers PXD000769 and PXD000768.
    Journal of Proteome Research 03/2014; · 5.06 Impact Factor
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    ABSTRACT: To assess whether inflammatory markers predict atherosclerotic disease activity after carotid treatment in patients with severe carotid stenosis and nonsignificant coronary artery disease undergoing carotid stenting. From March 1, 2004, to September 30, 2005, a total of 55 consecutive patients (mean ± SD age, 69±8.3 years; 26 men) with severe carotid stenosis and nonsignificant coronary artery disease were treated with carotid stent implantation. Patients were followed up for a period of 5 years for the occurrence of cardiovascular events. A significant correlation between quantitative analysis of debris entrapped in the filters and inflammatory markers was found. Moreover, the number of particles per filter, the total particles area, and the mean particle axis per filter were significantly higher in patients with clinical events at the follow-up compared with patients without events (87 vs 32, P=.006; 50,118.7 vs 17,782, P=.002; 33.9 vs 30.2, P=.03). At 5-year follow-up we recorded cardiovascular or neurologic events in 11 of the 55 patients (20%). Higher preprocedural levels of high-sensitivity C-reactive protein, interleukin 6 soluble receptor, and interleukin 6 were significantly associated with clinical events at follow-up (P<.001, P=.05, and P=.02, respectively). In particular high-sensitivity C-reactive protein measured at 24 and 48 hours after carotid stenting showed a significant correlation with clinical events (P=.001). Also preprocedural intracellular adhesion molecule 1 and circulating vascular cell adhesion molecule 1 blood concentrations were significantly correlated with a worse prognosis at follow-up (P=.04 and P=.03, respectively). In patients with severe carotid stenosis and nonsignificant coronary artery disease, inflammation is associated with atherosclerotic disease activity and a worse prognosis. Interleukin 6, interleukin 6 soluble receptor, intracellular adhesion molecule 1, vascular cell adhesion molecule 1, and high-sensitivity C-reactive protein levels at baseline and 24 and 48 hours after carotid stenting are predictive of neurologic and cardiovascular events at follow-up.
    Mayo Clinic Proceedings 01/2012; 87(1):50-8. · 5.79 Impact Factor
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    ABSTRACT: The Evaluation of Final Feasible Effect of Ultra Control Training and Sensitization (EFFECTUS) study is aimed at implementing global cardiovascular (CV) risk management in Italy. To evaluate the impact of diabetes mellitus (DM) on attitudes and preferences for clinical management of global CV risk among physicians treating diabetic or nondiabetic patients. Involved physicians were asked to submit data into a study-designed case-report form, covering the first 10 adult outpatients consecutively seen in May 2006. All available clinical data were centrally analyzed for global CV risk assessment and CV risk profile characterization. Patients were stratified according to the presence or absence of DM. Overall, 1078 physicians (27% female, ages 50 ± 7 y) collected data of 9904 outpatients (46.5% female, ages 67 ± 9 y), among whom 3681 (37%) had a diagnosis of DM at baseline. Diabetic patients were older and had higher prevalence of obesity, hypertension, dyslipidemia, and associated CV diseases than nondiabetic individuals (P<0.001). They had higher systolic blood pressure, total cholesterol, triglycerides, and creatinine levels, but lower high-density lipoprotein cholesterol levels than nondiabetic patients (P<0.001). Higher numbers of blood pressure and lipid-lowering drugs and antiplatelet agents were used in diabetic than in nondiabetic patients (P<0.001). The EFFECTUS study confirmed higher CV risk and more CV drug prescriptions in diabetic than in nondiabetic patients. Presence of DM at baseline significantly improved clinical data collection. Such an approach, however, was not paralleled by a better control of global CV risk profile, which was significantly worse in the former than in the latter group.
    Clinical Cardiology 09/2011; 34(9):560-6. · 1.83 Impact Factor
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    ABSTRACT: To evaluate the potential impact of physicians' age on global cardiovascular (CV) risk management in the population of the Evaluation of Final Feasible Effect of Ultra Control Training and Sensitisation (EFFECTUS) study. Involved physicians were stratified into three age groups (≤ 45, 46-55 and > 55 years), and asked to provide clinical data covering the first 10 adult outpatients, consecutively seen in May 2006. Overall 1078 physicians, among whom 219 (20%) were aged ≤ 45, 658 (61%) between 46 and 55, and 201 (19%) > 55 years, collected data of 9904 outpatients (46.5% female patients, aged 67 ± 9 years), who were distributed into three corresponding groups: 2010 (20%), 6111 (62%) and 1783 (18%), respectively. A higher prevalence of myocardial infarction and stroke was recorded by younger physicians rather than those aged > 46 years. Older physicians frequently recommended life-style changes, whereas a higher number of antihypertensive, antiplatelet, glucose and lipid-lowering prescriptions was prescribed by physicians aged ≤ 45 years. This analysis of the EFFECTUS study indicates a higher prevalence of vascular diseases among outpatients who were followed by younger physicians, who prescribed a higher number of CV drugs than older physicians. These older physicians have more attitude for prescribing favourable life-style changes than younger physicians.
    International Journal of Clinical Practice 06/2011; 65(6):649-57. · 2.43 Impact Factor
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    ABSTRACT: To examine levels of serum homocysteine (Hcy), vitamin B12 and folic acid in patients with pseudoexfoliation glaucoma (PEXG), primary open-angle glaucoma (POAG), and healthy control subjects. This study included 36 patients with PEXG, 40 with POAG, and 40 age-matched healthy subjects. Fasting plasma Hcy concentrations and levels of serum vitamin B12 and folic acid were measured using competitive chemiluminescent enzyme immunoassay; values exceeding 14 μm/l were considered elevated. Mean plasma Hcy was significantly higher in PEXG (16.55 ± 7.23 μm/l) compared with POAG (13.91 ± 3.61 μm/l) and controls (13.12 ± 5.13 μm/l) (p = 0.03 and p = 0.0007 respectively). There were no statistical differences in serum vitamin B12 and folic acid levels among PEXG, POAG and control subjects (p > 0.05). A moderate, although statistically significant, relationship between Hcy and folic acid levels was found in the PEXG group (R(2) = 0.23, p = 0.003). Hcy levels were found not to be related with folic acid or vitamin B12 in either POAG or control subjects. In this study, plasma Hcy is significantly higher in PEXG group than the POAG and control groups. Hyper-Hcy might play a role in the pathogenesis of PEXG. Hyper-Hcy may be an independent factor stressing vasculopathy in addition to pseudoexfoliation, so might be a modifiable risk factor for PEXG.
    Albrecht von Graæes Archiv für Ophthalmologie 03/2011; 249(3):443-8. · 1.93 Impact Factor
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    ABSTRACT: Cardiovascular diseases represent the leading cause of morbidity and mortality, worldwide. Early detection and appropriate management of cardiovascular risk factors and disease markers in daily clinical practice may improve preventive strategies and reduce the burden of cardiovascular disease. The EFFECTUS (Evaluation of Final Feasible Effect of Control Training and Ultra Sensitisation) programme was an educational programme aimed at evaluating prevalence of major cardiovascular risk factors among outpatients, and preferences and attitudes for cardiovascular disease management among Italian physicians in their routine clinical practice. This article provides an overview of the main findings of different analyses from the EFFECTUS database, which have demonstrated a high prevalence of cardiovascular risk factors, irrespective of the clinical settings and outpatient clinics in which patients were followed. Also, findings from this database suggest that more intensive clinical data recording was paralleled by better adherence to guidelines, and that use of electronic rather than conventional support for clinical data collection and registration improved accuracy in data recording, which translated into better management of patients at risk in daily clinical practice.
    High Blood Pressure & Cardiovascular Prevention 03/2011; 18(1):19-30.
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    ABSTRACT: In central nervous system cholesterol cannot be degraded but is secreted into circulation predominantly in the form of its polar metabolite 24(S)-hydroxycholesterol (24S-OH-Chol). Some studies suggested an association between 24S-OH-Chol metabolism and different neurological diseases including dementia. A possible decrease in 24S-OH-Chol plasma levels has been reported late onset Alzheimer's disease (LOAD) and vascular dementia (VD), but results of previous studies are partially contradictory. By high-speed liquid chromatography/tandem mass spectrometry we evaluated the plasma levels of 24S-OH-Chol in a sample of 160 older individuals: 60 patients with LOAD, 35 patients with VD, 25 subjects affected by cognitive impairment no-dementia (CIND), and 40 (144 for genetics study) cognitively normal Controls. We also investigated the possible association between PPARgamma Pro12Ala polymorphism and dementia or 24S-OH-Chol levels. Compared with Controls, plasma 24S-OH-Chol levels were higher in LOAD and lower in VD; a slight not-significant increase in CIND was observed (ANOVA p: 0.001). A positive correlation between 24S-OH-Chol/TC ratio and plasma C reactive protein (CRP) levels was found in the whole sample, independent of possible confounders (multiple regression p: 0.04; r2: 0.10). This correlation was strong in LOAD (r: 0.39), still present in CIND (r: 0.20), but was absent in VD patients (r: 0.08). The PPARgamma Pro12Ala polymorphism was not associated with the diagnosis of LOAD, VD, or CIND; no correlation emerged between the Ala allele and 24S-OH-Chol plasma levels. Our results suggest that plasma 24S-OH-Chol levels might be increased in the first stages of LOAD, and this phenomenon might be related with systemic inflammation. The finding of lower 24S-OH-Chol concentrations in VD might be related with a more advanced stage of VD compared with LOAD in our sample, and/or to different pathogenetic mechanisms and evolution of these two forms of dementia.
    BMC Neurology 01/2011; 11:121. · 2.56 Impact Factor
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    ABSTRACT: The Final Evaluation Feasible Effect of Ultra Control Training and Sensitization (EFFECTUS) is an educational program, aimed at improving global CV risk stratification and management in Italy. The present study evaluates differences on clinical approach to global CV risk among physicians involved in the EFFECTUS program and stratified in three geographical macro-areas (North, Center, South) of our Country. Physicians were asked to submit data already available in their medical records, covering the first 10 adult outpatients, consecutively seen in the month of May 2006. Overall, 1.078 physicians (27% females, aged 50 ± 7 years) collected data of 9.904 outpatients (46.5% females, aged 67 ± 9 years), among which 3.219 (32.5%) were residents in Northern, 3.652 (36.9%) in Central and 3.033 (30.6%) in Southern Italy. A significantly higher prevalence of major CV risk factors, including obesity, physical inactivity, hypertension and diabetes, was recorded in Southern than in other areas. Accordingly, Southern physicians more frequently prescribed antihypertensive, glucose and lipid lowering agents than other physicians, who paid significantly more attention to life-style changes in their clinical practice. This analysis of the EFFECTUS study demonstrates a high prevalence of CV risk factors in Italy, particularly in Southern areas, and indicates some important discrepancies in the clinical management of global CV risk among physcians working in different Italian regions.
    Nutrition, metabolism, and cardiovascular diseases: NMCD 12/2010; 22(8):635-42. · 3.52 Impact Factor
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    ABSTRACT: Plant sterols lower serum cholesterol concentration. Available data have confirmed the lipid-lowering efficacy in adults, while there is a relative dearth of data in children and almost exclusively restricted to subjects with familial hypercholesterolemia (FH). Aim of the present study was to evaluate the efficacy, tolerability and safety of plant sterol supplementation in children with different forms of primary hyperlipidemias. The effect of plant sterol consumption on plasma lipids was evaluated in 32 children with heterozygous FH, 13 children with Familial Combined Hyperlipidemia (FCH) and 13 children with Undefined Hypercholesterolemia (UH) in a 12-week open-label intervention study using plant sterol-enriched yoghurt. Plasma lipids and apolipoproteins were measured by routine methods. Markers of cholesterol synthesis (lathosterol) and absorption (campesterol and sitosterol) were measured by GC-MS. Tolerability and adherence to recommended regimen was very high. A significant reduction was observed in LDL-cholesterol in the three groups (10.7, 14.2 and 16.0% in FH, FCH and UH, respectively). Lathosterol concentrations were unchanged, reflecting a lack of increased synthesis of cholesterol. Of the two absorption markers, only sitosterol showed a slight but significant increase. Daily consumption of plant sterol dairy products favorably changes lipid profile by reducing LDL-cholesterol. To our knowledge, this is the first report of the use of plant sterols-enriched foods in treating children with primary hyperlipidemia such as FCH and UH, likely to be the most frequent form also in the young age in the western populations.
    Acta Diabetologica 11/2010; 48(2):127-33. · 4.63 Impact Factor
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    ABSTRACT: Platelets, the smallest human blood cells component, have a key role in the control of haemostasis and thrombosis but they have also been shown to be implicated in a number of different pathological states because of their involvement also in the process of inflammation end its resolution. Their peculiar anucleated morphology render the proteomics an intriguing approach to understand their biology. Given the high impact of platelet in different diseases we have started a systematic investigation of protein repertoire in controlled platelet preparation. Platelets have been extracted from blood of healthy donors (n=6) collected by venipuncture in Vacutainer. The quality of the preparation was assessed by observation and enumeration in a Bürker chamber with a conventional tissue culture microscope. To characterize human platelets proteome we analysed the pool of purified platelets combining two proteomic approaches: 2-DE separation combined with Mass Spectrometry and nanoscale ultra performances LC-MS(E) shotgun proteomics experiments. The 2D gel analysis leads an average of 1900 protein spots, after the filtering of "noise" and "false positive" spots, over 500 were selected to be eligible for further analysis given their optimal spot quality value. To perform the analysis by ion accounting shotgun proteomic approach, based on nano ultra performance liquid chromatography (nUPLC) coupled to MS(E) processing of continuum LC-MS data, the same pool of samples was subject to liquid phase tryptic digestion and the peptide obtained used for the experiments. All the data obtained were analysed using ProteinLynx GlobalServer v2.3 (PLGS, Waters). Three analytical replicates run were acquire in high/low energy modes and associated to a human protein database returning the identification of 100 distinct genes. Comparative analysis of the Gene Ontology has been performed to evaluate the differential functional representation of the molecular repertoire investigated with these two orthogonal approaches. The overall molecular function classification revealed differences between the two proteomic approaches. In particular, we found significant differences in cytoskeletal proteins (19.65% 2-DE versus 45.60 Shotgun) and receptors (0,92% 2-DE versus 6.90% Shotgun).
    Blood transfusion = Trasfusione del sangue 06/2010; 8 Suppl 3:s140-8. · 1.86 Impact Factor
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    ABSTRACT: Introduction The EFFECTUS (Evaluation of Final Feasible Effect of Control Training and Ultra Sensitisation) study is an educational programme, aimed at implementing global cardiovascular risk management in daily clinical practice in Italy.Objective To evaluate global cardiovascular risk management among physicians stratified according to the use of conventional (CS) or electronic (ES) support for clinical data collection and registration.Methods Involved physicians were asked to submit data into a study-designed, case-report form, covering the first ten adult outpatients consecutively seen in May 2006. A case-report form was made available on CS or ES, depending on physicians' preferences and attitudes. All available data were centrally analysed for global cardiovascular risk assessment and cardiovascular risk profile characterization.Results Overall, 1078 physicians (27% females, aged 50 ± 7 years) collected data from 9904 outpatients (46.5% females, aged 67 ± 9 years). 299 physicians used CS for 2672 (27.0%) patients, whereas the remaining 779 physicians used ES for 7232 patients (73.0%). A higher prevalence of obesity, diabetes mellitus, ischaemic heart disease (mostly previous myocardial infarction) and stroke, was recorded by physicians using CS compared with those using ES. Blood pressure and fasting glucose levels were significantly higher in the CS group than in the ES group, although a significantly higher number of antihypertensive, glucose and lipid-lowering prescriptions was reported in the former than in the latter group. Physicians using ES paid significantly more attention to collecting data concerning cardiovascular risk factors, which were also more up-to-date compared with those recorded by physicians using CS.Conclusions This sub-analysis of the EFFECTUS study indicates a higher attention to the collection and management of data on major cardiovascular risk factors in physicians using ES than in those using CS. Our findings may suggest a potential way to improve global cardiovascular risk management in the clinical practices of Italy.Received for publication 8 February 2010; accepted for publication 14 March 2010.
    High Blood Pressure & Cardiovascular Prevention 02/2010; 17(1):37-47.
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    ABSTRACT: Introduction: The EFFECTUS (Evaluation of Final Feasible Effect of Control Training and Ultra Sensitisation) study is an educational programme, aimed at implementing global cardiovascular risk management in daily clinical practice in Italy. Objective: To evaluate global cardiovascular risk management among physicians stratified according to the use of conventional (CS) or electronic (ES) support for clinical data collection and registration. Methods: Involved physicians were asked to submit data into a study-designed, case-report form, covering the first ten adult outpatients consecutively seen in May 2006. A case-report form was made available on CS or ES, depending on physicians’ preferences and attitudes. All available data were centrally analysed for global cardiovascular risk assessment and cardiovascular risk profile characterization. Results: Overall, 1078 physicians (27% females, aged 50 ± 7 years) collected data from 9904 outpatients (46.5% females, aged 67 ± 9 years). 299 physicians used CS for 2672 (27.0%) patients, whereas the remaining 779 physicians used ES for 7232 patients (73.0%). A higher prevalence of obesity, diabetes mellitus, ischaemic heart disease (mostly previous myocardial infarction) and stroke, was recorded by physicians using CS compared with those using ES. Blood pressure and fasting glucose levels were significantly higher in the CS group than in the ES group, although a significantly higher number of antihypertensive, glucose and lipid-lowering prescriptions was reported in the former than in the latter group. Physicians using ES paid significantly more attention to collecting data concerning cardiovascular risk factors, which were also more up-to-date compared with those recorded by physicians using CS. Conclusions: This sub-analysis of the EFFECTUS study indicates a higher attention to the collection and management of data on major cardiovascular risk factors in physicians using ES than in those using CS. Our findings may suggest a potential way to improve global cardiovascular risk management in the clinical practices of Italy.
    High Blood Pressure & Cardiovascular Prevention 01/2010; 17(1):37-47.
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    ABSTRACT: Physicians' adherence to cardiovascular (CV) guidelines has been found to be poor. In this regard, accuracy in keeping medical records could play an important role. This study was devised to describe which data are present in medical records from a large sample of physicians and to investigate the association and the link between completeness in recording and clinical appropriateness. The data extracted from medical records of 1078 doctors (general practitioners, cardiologists, and diabetologists) were analyzed, with a focus on CV prevention. The percentage of recorded data of several CV clinical variables was calculated. A multivariate analysis was performed to investigate the association between doctors' and patients' characteristics and different patterns in recording. Finally, the completeness in recording was calculated with a score and plotted against three indicators of appropriateness. The only risk factor that achieved a good standard of registration was blood pressure (89%). Low-density lipoprotein and waist circumference were largely under-recorded, whereas lifestyle data collection was almost negligible. Age, specialization, and use of electronic records increase the accuracy in recording. When one CV risk factor was predominant, the probability of having other risk factors recorded was reduced. A significant increase in the proportion of patients treated according to guidelines was found in doctors who were more accurate in recording. A link exists between accuracy in recording with both quality of care and adherence to guidelines. Specific training of all doctors in this field should be considered.
    European journal of cardiovascular prevention and rehabilitation: official journal of the European Society of Cardiology, Working Groups on Epidemiology & Prevention and Cardiac Rehabilitation and Exercise Physiology 09/2009; 16(6):722-8. · 2.51 Impact Factor
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    ABSTRACT: To set-up a method for a direct evaluation in human serum of paraoxonase enzymatic activities, establishing a possible correlation with Q192R genotype polymorphism. 101 different human serum samples were genotyped for paraoxonase Q192R polymorphism by PCR restriction analysis, and evaluated spectrophotometrically with regard to paraoxon and 2-coumaranone hydrolytic activities. Both activities of paraoxonase were assayed, quantified through normalization by arylesterase activity, and compared with the data concerning Q/R genetic polymorphism. The mean normalized paraoxonase activity was found to be significantly higher in RR than in QQ human sera (3.99+/-0.6 versus 1.32+/-0.44; P<0.0001); instead, the 2-coumaranone hydrolysis showed an opposite trend (0.10+/-0.02 versus 0.23+/-0.04, in RR and QQ sera respectively; P<0.0001). These methods were successfully applied to the whole serum, suggesting a possible use of this approach for a clinically relevant phenotypic characterization.
    Clinical biochemistry 08/2009; 42(15):1512-6. · 2.02 Impact Factor
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    ABSTRACT: Background Cardiovascular diseases still represent the leading cause of morbidity and mortality, worldwide. Early detection and appropriate management of major cardiovascular risk factors in clinical practice may improve preventive strategies in Western countries, including Italy.Objective To evaluate the prevalence of major cardiovascular risk factors and their impact on routine clinical practice of Italian general practitioners (GPs), cardiologists and diabetologists.Methods The study involved physicians who participated in an educational project on cardiovascular risk management, the EFFECTUS (Evaluation of Final Feasible Effect of Control Training and Ultra-Sensitisation) programme. Physicians were asked to report data already available in their clinical records of the first ten consecutive adult outpatients during May 2006. Data collection included patients' full medical history and therapies, physical examination (anthropometric parameters and blood pressure levels), laboratory results and diagnostic examinations. Data were then centrally analysed for global cardiovascular risk evaluation and cardiovascular risk profile characterization. The present study provides data from the baseline records of the EFFECTUS programme.Results A total of 1078 physicians (73% males and 27% females, mean age 50 ± 7 years), of which 841 (78%) were GPs, 140 (13%) cardiologists and 97 (9%) diabetologists, included data from 9904 outpatients (5300 males and 4604 females, mean age 67 ± 9 years). In the overall population, 2504 (25%) subjects were obese, 7436 (75%) had hypertension, 5873 (59%) had dyslipidaemia, 3681 (37%) had diabetes mellitus and 2633 (27%) had a history of ischaemic heart disease, while 1102 (11%) and 1247 (13%) had cerebral or peripheral artery disease, respectively. Significant differences were reported in the prevalence of detected cardiovascular risk factors among physicians operating in different clinical settings. Moreover, different clinical habits were recorded.Conclusions The present analysis illustrates a very high prevalence of cardiovascular risk factors, irrespective of the clinical settings in which patients were followed, in a vast Italian population. The study also describes differences in management of cardiovascular risk factors among physicians operating in different clinical settings.
    High Blood Pressure & Cardiovascular Prevention 06/2009; 16(2):55-63.
  • Atherosclerosis Supplements 06/2009; 10(2). · 4.33 Impact Factor
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    ABSTRACT: Body fatness and fat distribution are widely accepted as coronary heart disease risk factors. In this study, we have evaluated the contribution of generalized adiposity, assessed by body mass index (BMI), to carotid atherosclerosis, in participants with or without metabolic syndrome (MetS). We have analysed 840 female and 1002 male participants in a regional Cardiovascular Disease Prevention Campaign. Blood glucose and lipids were analysed by standard methods. According to BMI, calculated as weight (in kilograms)/height (in square metres), participants were divided into normal weight (BMI <25 kg/m2), overweight (BMI between 25 and 29.9 kg/m2) and obese (BMI>29.9 kg/m2). Carotid atherosclerosis was evaluated by echo Doppler. Blood pressure, waist circumference, triglycerides and glucose were significantly higher, and high-density lipoprotein was lower, in overweight and obese participants, compared with normal weight. MetS was more frequent among obese and overweight than normal-weight participants (51.7 vs. 21.5 vs. 9.8%, respectively). The prevalence of carotid atherosclerosis was 45.29% in participants with MetS, significantly higher than in participants without MetS (33.04%, P<0.0001), but it was similar across the three weight categories. Furthermore, in multiple regression analyses BMI was not significantly associated with carotid atherosclerosis. The present findings suggest that increasing body weight favours the clustering of coronary heart disease risk factors. Overweight and obesity, however, do not independently associate with carotid atherosclerosis.
    Coronary artery disease 03/2009; 20(2):94-9. · 1.56 Impact Factor
  • High Blood Pressure & Cardiovascular Prevention 01/2009; 16(2):55-63.
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    ABSTRACT: Stromelysin (MMP3), through its action on collagen and other matrix metalloproteinases, influences arterial wall remodeling. In healthy subjects, the 5A/6A polymorphism located in the promoter of the MMP3 gene is associated with common carotid remodeling, 6A/6A subjects having increased arterial diameter, wall thickness (intima-media thickness, IMT) and decreased wall shear stress (WSS). In the present study, we have investigated the influence of the 5A/6A polymorphism on common carotid remodeling in subjects with diabetes mellitus. Diabetic subjects (N.=136) and age-matched healthy male controls (N.=101) have been studied. Common carotid diameter, IMT and flow velocity have been measured by echo-Doppler. Blood viscosity has been measured by a cone/plate viscometer. WSS has been calculated. Diabetic patients had increased common carotid diameter, IMT, and decreased flow velocity and WSS (all P<0.05), compared with controls. In controls, subjects homozygous for the 6A allele had increased diameter, IMT and decreased WSS. In diabetics, no difference was observed in vascular parameters among the three genotypes. The 5A/6A polymorphism of the MMP3 gene influences arterial remodeling of the common carotid artery in healthy subjects, but not in patients with diabetes mellitus. Therefore, the significance of the 5A/6A polymorphism as a marker of risk in this high cardiovascular risk population seems to be somehow blunted.
    International angiology: a journal of the International Union of Angiology 10/2008; 27(5):413-8. · 1.46 Impact Factor
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    ABSTRACT: Plant sterols, added to several food sources, lower serum cholesterol concentrations. Plant sterol-induced cholesterol lowering is paralleled by a mild decrease in plasma levels of the antioxidant beta-carotene, the amount of this decrease being considered clinically non-significant. Whether the effect on lipid profile of daily consumption of plant sterol-enriched low-fat fermented milk (FM) is paralleled by a concomitant variation in a reliable marker of the oxidative burden like plasma isoprostane levels is unresolved. The effect of plant sterol consumption on plasma lipid and isoprostane levels of hypercholesterolemic patients was evaluated in a multicenter, randomized double blind study. Hypercholesterolemic patients consumed a FM daily for 6 weeks. Subjects were randomized to receive either 1.6g of plant sterol-enriched FM (n=60) or control FM product (n=56). After 6 weeks of plant sterol-enriched FM consumption, LDL cholesterol was reduced from 166.2+/-2.0 to 147.4+/-2.8 mg/dL (p=0.01). A significant reduction was observed for total cholesterol (from 263.5+/-2.6 to 231.0+/-3.2mg/dL, p=0.01). There was greater LDL cholesterol lowering among hypercholesterolemic patients with higher LDL cholesterol at baseline. We found a reduction of plasma 8-isoprostane in patients taking plant sterol-enriched FM (from 43.07+/-1.78 to 38.04+/-1.14 pg/ml, p=0.018) but not in patients taking the control product (from 42.56+/-2.12 to 43.19+/-2.0 pg/ml, p=NS). Campesterol and beta-sitosterol levels were not influenced by phytosterol consumption. Daily consumption of low-fat plant sterol dairy product favourably changes lipid profile by reducing LDL-cholesterol, and may also have an anti-oxidative effect through a reduction of plasma isoprostanes.
    Nutrition, metabolism, and cardiovascular diseases: NMCD 09/2008; 19(2):84-90. · 3.52 Impact Factor

Publication Stats

1k Citations
302.60 Total Impact Points

Institutions

  • 2011
    • Sant'Andrea Medical Hospital
      Spezia, Liguria, Italy
  • 2010
    • Università degli Studi di Torino
      Torino, Piedmont, Italy
  • 1998–2010
    • University of Rome Tor Vergata
      • • Dipartimento di Medicina dei Sistemi
      • • Dipartimento di Biologia
      Roma, Latium, Italy
  • 1999–2009
    • Universita' degli Studi "Magna Græcia" di Catanzaro
      • Department of Experimental and Clinical Medicine "Gaetano Salvatore"
      Catanzaro, Calabria, Italy
  • 2006
    • Policlinico Tor Vergata
      • Medicina Interna
      Roma, Latium, Italy
  • 1998–2005
    • Ospedale Pediatrico Bambino Gesù
      Roma, Latium, Italy
  • 2001
    • Università degli Studi di Teramo
      Teramo, Abruzzo, Italy
  • 2000
    • Istituto Superiore di Sanità
      • Department of Haematology, Oncology and Molecular Medicine
      Roma, Latium, Italy
  • 1990–2000
    • Second University of Naples
      Caserta, Campania, Italy
  • 1997
    • University of Camerino
      Camerino, The Marches, Italy
  • 1989
    • Mediterranean University of Reggio Calabria
      Reggio di Calabria, Calabria, Italy