Chloe Spichiger-Haeusermann

Publications (2)19.43 Total impact

• Article: Coop functions as a corepressor of Pangolin and antagonizes Wingless signaling.

  Haiyun Song, Sandra Goetze, Johannes Bischof, Chloe Spichiger-Haeusermann, Marco Kuster, Erich Brunner, Konrad Basler
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  ABSTRACT: Wingless (Wg) signaling regulates expression of its target genes via Pangolin and Armadillo, and their interacting cofactors. In the absence of Wg, Pangolin mediates transcriptional repression. In the presence of Wg, Pangolin, Armadillo, and a cohort of coactivators mediate transcriptional activation. Here we uncover Coop (corepressor of Pan) as a Pangolin-interacting protein. Coop and Pangolin form a complex on DNA containing a Pangolin/TCF-binding motif. Overexpression of Coop specifically represses Wg target genes, while loss of Coop function causes derepression. Finally, we show that Coop antagonizes the binding of Armadillo to Pangolin, providing a mechanism for Coop-mediated repression of Wg target gene transcription.
  Genes & development 05/2010; 24(9):881-6. · 12.08 Impact Factor
• Article: The ISWI-containing NURF complex regulates the output of the canonical Wingless pathway.

  Haiyun Song, Chloe Spichiger-Haeusermann, Konrad Basler
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  ABSTRACT: Wingless (Wg) signalling regulates the expression of its target genes through Pangolin, Armadillo and their interacting co-factors. In a genetic screen for Wg signalling components, we found that imitation switch (ISWI), a chromatin-remodelling ATPase, had a positive role in transducing the canonical Wg signal, promoting the expression of the Wg target senseless. ISWI is found in several chromatin-remodelling complexes, including nucleosome remodelling factor (NURF). The effect of interfering with the function of other components of the NURF complex in vivo mimics that of ISWI. The NURF complex is also required for the efficient expression of other Wg target genes. Armadillo interacts directly with the NURF complex in vitro and recruits it to Wg targets in cultured cells. Together, our results suggest that the ISWI-containing NURF complex functions as a co-activator of Armadillo to promote Wg-mediated transcription.
  EMBO Reports 09/2009; 10(10):1140-6. · 7.36 Impact Factor