Fan-Xu Meng

State Oceanic Administration, Tsingtao, Shandong Sheng, China

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Publications (7)12.64 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: A Gram-negative, short rod-shaped bacterium, designated 26DY36(T), was isolated from a sediment sample collected from the North Atlantic Rise. The isolate required NaCl and grew best with 2 % (w/v) sea salts at temperature of between 30 and 35 (o)C at pH 7.0. It formed yellow colonies, produced carotenoid-like pigments and did not produce bacteriochlorophyll a. Strain 26DY36(T) was positive for hydrolysis of aesculin, gelatin, tyrosine and Tween 20, 40, 60 and 80, and negative for hydrolysis of casein, DNA and starch. The major respiratory quinone was ubiquinone-10. The major polar lipid profile consisted of sphingoglycolipid, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and two unidentified glycolipids. The principal fatty acids (>5 %) were C18:1ω7c, C17:1ω6c, C15:0 2OH and C16:0. The genomic DNA G + C content was 59.4 mol%. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain 26DY36(T) should be assigned to the genus Altererythrobacter. The 16S rRNA gene sequence similarities between the isolate and the type strains of Altererythrobacter species were in the range 92.7 - 96.5 %. On the basis of phenotypic and genotypic data, strain 26DY36(T) represents a novel species of the genus Altererythrobacter, for which the name Altererythrobacter atlanticus sp. nov. (type strain 26DY36(T) = CGMCC 1.12411(T) = JCM 18865(T)) is proposed.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 09/2013; · 2.11 Impact Factor
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    ABSTRACT: Deep-sea polymetallic nodules, rich in metals such as Fe, Mn, and Ni, are potential resources for future exploitation. Early culturing and microscopy studies suggest that polymetallic nodules are at least partially biogenic. To understand the microbial communities in this environment, we compared microbial community composition and diversity inside nodules and in the surrounding sediments. Three sampling sites in the Pacific Ocean containing polymetallic nodules were used for culture-independent investigations of microbial diversity. A total of 1013 near full-length bacterial 16S rRNA gene sequences and 640 archaeal 16S rRNA gene sequences with ~650 bp from nodules and the surrounding sediments were analyzed. Bacteria showed higher diversity than archaea. Interestingly, sediments contained more diverse bacterial communities than nodules, while the opposite was detected for archaea. Bacterial communities tend to be mostly unique to sediments or nodules, with only 13.3% of sequences shared. The most abundant bacterial groups detected only in nodules were Pseudoalteromonas and Alteromonas, which were predicted to play a role in building matrix outside cells to induce or control mineralization. However, archaeal communities were mostly shared between sediments and nodules, including the most abundant OTU containing 290 sequences from marine group I Thaumarchaeota. PcoA analysis indicated that microhabitat (i.e., nodule or sediment) seemed to be a major factor influencing microbial community composition, rather than sampling locations or distances between locations.
    Deep Sea Research Part I: Oceanographic Research Papers. 09/2013; 79:40–49.
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    ABSTRACT: A Gram-negative, short rod-shaped bacterium, designated 22DY03(T), was isolated from a sediment sample collected from the East Pacific Rise. The isolate required NaCl and grew best with 3-7 % (w/v) sea salts at temperature of between 30 and 35 (o)C within pH 7.0. It formed non-pigmented colonies and produced exopolysaccharide, but did not produce Bchl a. Strain 22DY03(T) was positive for glucose fermentation as well as hydrolysis of aesculin and Tween 20, and negative for hydrolysis of casein, DNA, gelatin, starch and Tween 40, 60 and 80. The major respiratory quinone was ubiquinone-10. The polar lipid profile consisted of a mixture of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol, two unidentified phospholipids and four unidentified polar lipids. The major fatty acids were C19:0 cyclo ω8c, C18:1ω7c and 11-methy C18:1ω7c. The genomic DNA G + C content was 64.6 mol%. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain 22DY03(T) should be assigned to the genus Roseivivax. The 16S rRNA gene sequence similarities between the isolate and the type strains of Roseivivax species were in the range 94.1-95.8 %. On the basis of phenotypic and genotypic data, strain 22DY03(T) represents a novel species of the genus Roseivivax, for which the name Roseivivax pacificus sp. nov. (type strain 22DY03(T) = CGMCC 1.12410(T) = JCM 18866(T)) is proposed.
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 08/2013; · 2.11 Impact Factor
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    ABSTRACT: A Gram stain-negative, aerobic, rod-like, motile by peritrichous flagella and moderately halophilic bacterium, designated strain B6(T), was isolated a deep-sea sediment collected from the South Atlantic Ocean. The isolate grew with 0.5-15% (w/v) NaCl, 4-37 (o)C and pH 5.0-8.5, and showed a high tolerance to zinc, manganese, cobalt and copper ions. The major fatty acids were C16:0, C19:0 cyclo ω8c and C12:0. The predominant ubiquinone was Q-9. The genomic DNA G+C content was 61.1 mol%. Phylogenetic analysis based on 16S rRNA gene comparisons indicated that strain B6(T) belonged to the genus Halomonas, and the closest relative was Halomonas xinjiangensis TRM 0175(T) (96.1%). Based upon the phenotypic, chemotaxonomic and genetic data, strain B6(T) represents a novel species in the genus Halomonas, for which the name Halomonas zincidurans sp. nov. is proposed. The type strain is B6(T) (= CGMCC 1.12450(T) = JCM 18472(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 06/2013; · 2.11 Impact Factor
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    ABSTRACT: A Gram stain-negative, aerobic and rod-shaped bacterium, strain DY22(T), was isolated from a deep-sea sediment collected from the east Pacific Ocean. The isolate was found to grow in the presence of 0-20.0 % (w/v) NaCl and at pH 4.5-8.5; optimum growth was observed with 0.5-2.0 % (w/v) NaCl and at pH 5.0-7.0. Chemotaxonomic analysis showed the presence of ubiquinone-9 as predominant respiratory quinone and C16:0, C19:0 ω8c cyclo and C12:0 3-OH as major cellular fatty acids. The genomic DNA G+C content was determined to be 59.6 mol%. Comparative 16S rRNA gene sequence analysis revealed that the novel isolate belongs to the genus Salinicola. Strain DY22(T) exhibited the closest phylogenetic affinity to the type strain of Salinicola salarius with 97.2 % sequence similarity and less than 97 % sequence similarity with respect to other Salinicola species with validly published names. The DNA-DNA reassociation values between strain DY22(T) and S. salarius DSM 18044(T) was 52 ± 4 %. On the basis of phenotypic, chemotaxonomic and genotypic data, strain DY22(T) represents a novel species of the genus Salinicola, for which the name Salinicola peritrichatus sp. nov. (type strain DY22(T) = CGMCC 1.12381(T) = JCM 18795(T)) is proposed.
    Antonie van Leeuwenhoek 04/2013; · 2.07 Impact Factor
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    ABSTRACT: A Gram-stain-positive, neutrophilic, rod-shaped bacterium, strain A1g(T), was isolated from activated sludge of a bioreactor and was subjected to a polyphasic taxonomic characterization. The isolate grew in the presence of 0-17.0 % (w/v) NaCl and at pH 6.0-9.0; optimum growth was observed in the presence of 3.0-5.0 % (w/v) NaCl and at pH 7.0. Strain A1g(T) was motile, formed cream-coloured colonies, was catalase- and oxidase-positive and was able to hydrolyse aesculin, Tween 40 and Tween 60. Chemotaxonomic analysis revealed menaquinone-7 as the predominant respiratory quinone and anteiso-C₁₅:₀, anteiso-C₁₇:₀, iso-C₁₆:₀ and iso-C₁₅:₀ as major fatty acids. The genomic DNA G+C content of strain A1g(T) was 36.3 mol%. Comparative 16S rRNA gene sequence analysis revealed that the new isolate belonged to the genus Oceanobacillus and exhibited closest phylogenetic affinity to the type strains of Oceanobacillus oncorhynchi subsp. incaldanensis (97.9 % similarity) and O. oncorhynchi subsp. oncorhynchi (97.5 %), but less than 97 % sequence similarity with respect to the type strains of other recognized Oceanobacillus species. Levels of DNA-DNA relatedness between strain A1g(T) and reference strains O. oncorhynchi subsp. incaldanensis DSM 16557(T), O. oncorhynchi subsp. oncorhynchi JCM 12661(T) and Oceanobacillus iheyensis DSM 14371(T) were 29, 45 and 38 %, respectively. On the basis of phenotypic and genotypic data, strain A1g(T) is considered to represent a novel species of the genus Oceanobacillus, for which the name Oceanobacillus neutriphilus sp. nov. is proposed. The type strain is A1g(T) (=CGMCC 1.7693(T) =JCM 15776(T)).
    INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY 11/2009; 60(Pt 10):2409-14. · 2.11 Impact Factor
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    ABSTRACT: A Gram-positive, neutrophilic, non-motile and non-spore-forming actinobacterium, strain FS24(T), was isolated from a bioreactor treating salt-containing wastewater. This isolate grew in the presence of 0-15 % (w/v) NaCl and at 10-37 degrees C. The optimum NaCl concentration for growth of FS24(T) was 5 % (w/v) at 37 degrees C or 1 % (w/v) at 25 degrees C. Chemotaxonomic analysis revealed MK-9(H(2)) as the predominant menaquinone and the major cellular polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, four unknown glycolipids, two unknown phospholipids and an unknown lipid. The major fatty acids were anteiso-C(15 : 0 ), iso-C(15 : 0), iso-C(16 : 0 ) and anteiso-C(17 : 0). The genomic DNA G+C content was 66.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain FS24(T) clustered with members of the genus Citricoccus, exhibiting high sequence similarity to the 16S rRNA gene sequences of the type strains of Citricoccus alkalitolerans (98.9 %) and Citricoccus muralis (98.8 %), respectively. The DNA-DNA relatedness values of strain FS24(T) to C. alkalitolerans DSM 15665(T) and C. muralis DSM 14442(T) were 54 and 39 %, respectively. On the basis of phenotypic and genotypic data, strain FS24(T) represents a novel species of the genus Citricoccus, for which the name Citricoccus zhacaiensis sp. nov. is proposed. The type strain is FS24( T) (=CGMCC 1.7064(T) =JCM 15136(T)).
    International journal of systematic and evolutionary microbiology 09/2009; 60(Pt 3):495-9. · 2.11 Impact Factor

Publication Stats

16 Citations
12.64 Total Impact Points

Institutions

  • 2013
    • State Oceanic Administration
      Tsingtao, Shandong Sheng, China
    • Second Institute of Oceanography SOA
      Hang-hsien, Zhejiang Sheng, China
  • 2009
    • Zhejiang University
      • College of Life Sciences
      Hangzhou, Zhejiang Sheng, China