Neuza Maria Souza Picorelli Assis

Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil

Are you Neuza Maria Souza Picorelli Assis?

Claim your profile

Publications (14)9.78 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: To evaluate the presence of the anterior extension (AE) of the mandibular canal and to measure the bone height (BH) at different points along the extension. Cone-beam computed tomography examinations of 108 patients were bilaterally evaluated regarding the presence of the AE, and linear measurements of the AE and BH were obtained. For comparisons between sides and genders, Student t test was applied. Variations in BH measurements were assessed by a 2-way analysis of variance. Through a simple linear regression analysis, formulas were determined for calculations of the BH in the AE. A right AE was detected in 76.9% and left AE in 77.8% of the samples. In the majority of the cases, the extension was of the rectilinear type. The mean length of the AE was 8.5 mm, and there was no statistically significant difference observed in relation to gender or side. It was observed that the BH increases as it approaches the midline. The AE of the mandibular canal must be evaluated during presurgical planning. The formulae presented in this study provide a method to calculate BH values in the interforaminal region and can be used to assist in presurgical planning, especially in the cases in which the identification of the hipodense site, which corresponds to the AE, is not evident.
    Implant dentistry 04/2015; 24(2):192-6. DOI:10.1097/ID.0000000000000226 · 1.11 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Numerous factors may keep surgeons from placing implants in the anterior maxilla in order to avoid suboptimal restorative outcomes. This paper describes a technique of an autogenous-free gingival-bone block graft, which allows bone and gingival augmentation and a primary seal to be achieved simultaneously. Additionally, it describes a technique for achieving primary soft tissue closure of maxillary extraction sockets using a rotated pedicle palatal connective tissue flap.
    Journal of Oral Implantology 04/2014; 40(2):183-187. DOI:10.1563/AAID-JOI-D-12-00016 · 0.98 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Several studies have suggested a link between periodontal disease and preterm birth, but the mechanism of how this occurs remains controversial. Therefore, this study aimed to investigate whether periodontal disease, defined according to two commonly used clinical definitions, is associated with preterm birth and to examine the association regarding oral health-related behaviors during pregnancy. This case-control study included women 18-40 years of age. Demographic and socio-economic data, information on current and previous pregnancies, and data on dental health-related behaviors and periodontal clinical parameters were collected within 48 h postpartum. Periodontal disease was assessed according to two definitions: four or more teeth with at least one site showing a probing depth of ≥ 4 mm and clinical attachment level of ≥ 3 mm (Definition 1); or at least one site with probing depth and clinical attachment level of ≥ 4 mm (Definition 2). The chi-square test was used to examine differences in the proportion of categorical variables. Bivariate analysis was performed to analyze the proportion of preterm births with respect to independent variables. Multiple logistic regression analyses were used to assess the association between periodontal disease and preterm birth. Odds ratios (ORs) were calculated with a 95% confidence interval (95% CI). A total of 296 postpartum women met the inclusion criteria. The case group included 74 women who delivered a preterm neonate (< 37 wk of gestation) and the control group included 222 women with deliveries at term (≥ 37 wk). Periodontal disease according to Definition 1 was not associated with fewer weeks of gestation (adjusted OR (ORadjusted ) = 1.62; 95% CI = 0.80-3.29; p = 0.178). However, a significant association was found between periodontal disease, according to Definition 2, and preterm birth (ORadjusted = 1.98; 95% CI = 1.14-3.43; p = 0.015). Increased appetite and a low number of daily toothbrushings were associated with preterm birth, regardless of the definition of periodontal disease used. Periodontal disease defined according to Definition 2 and unfavorable oral health-related behavior were factors associated with preterm birth.
    Journal of Periodontal Research 08/2013; DOI:10.1111/jre.12124 · 2.22 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to evaluate the influence of changes in maxillomandibular positioning during cone beam computed tomography (CBCT) imaging on the planning of dental implants. Ten skulls were marked bilaterally with metal spheres in four regions: incisors, canine, premolars, and molars. CBCT scans were obtained in seven positions: standard position (SP), displacements of 10° and 20° above and below the SP, and lateral displacements of 10° and 20° from the SP. Subsequently, bilateral measurements of the height and width of the maxilla and mandible were performed on all images. The results showed that the position with a displacement of 20° above the SP presented the greatest differences in the measurements of bone height and width. In the bilateral comparisons, the maxillary bone width showed the greatest differences, especially for the regions of the premolars and molars. It is concluded that alterations of positioning during the acquisition of CBCT images can lead to alterations in the measurements of bone height and width, which may result in errors in implant planning and cause damage to anatomical structures.
    International Journal of Oral and Maxillofacial Surgery 07/2013; 42(7):880–886. DOI:10.1016/j.ijom.2013.03.001 · 1.36 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Periodontal disease is one of the most prevalent oral diseases. An association between this disease and pregnancy has been suggested, but available findings are controversial. We evaluated the expression levels of interleukins (IL-1β and IL-6), tumor necrosis factor-alpha (TNF-α), and inducible nitric oxide synthase (iNOS) in pregnant women with and without periodontal disease in comparison with non-pregnant women with and without periodontal disease since studies have suggested a relationship between periodontitis and the expression levels of these genes. The women in the sample were distributed into four groups: pregnant and non-pregnant women, with or without periodontal disease, a total of 32 women. The periodontal condition was evaluated according to the probing depth, clinical attachment level and bleeding on probing. Analysis of gene expression was performed by real-time PCR. Comparisons were made of the level of gene expression among the four groups. Expression of IL-1β in the non-pregnant women with periodontal disease was 12.6 times higher than in the non-pregnant women without periodontal disease (P < 0.01), while expression of TNF-α in the non-pregnant women without periodontal disease was 3.5 times higher than in the pregnant women with periodontal disease (P < 0.05). Despite these differences, our overall findings indicate no differences in the expression levels of the cytokines IL-1β, IL-6, TNF-α, and iNOS in pregnant women with and without periodontal disease in comparison with expression of the same genes in non-pregnant women with and without periodontal disease, suggesting that periodontal disease is not influenced by pregnancy.
    Genetics and molecular research: GMR 09/2012; 11(AOP). DOI:10.4238/2012.September.20.3 · 0.85 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Objective: Dental implantology based on osseointegration is an established treatment option and new imaging methods have been used to ensure a more accurate planning. Cone beam computed tomography (CBCT) has now become an important tool for preimplantation assessment. The aim of this study was to evaluate the influence of changes in the maxilo-mandibular positioning during the acquisition of the CBCT ortorradial cuts, for planning of dental implants. Method: We used ten skulls, which received, bilaterally, metallic markers in four distinct regions: incisors, canines, premolars and molars. Tomographic scans of the skulls were obtained in seven positions: standard (PN), with displacements above and below 10° and 20° (P10 +, P20 +, P10- and P20-), as well as lateral displacement of 10° and 20° (PL10 and PL20). Subsequently, measurements of implant bone height and thickness in standard images and for those obtained with displacements were performed in four distinct regions identified by markers, bilaterally, for both maxilla and jaw. Result: The results showed that P20+ position presented the greatest difference in mean measures of bone height and thickness, when compared to the NP position. In bilateral comparison, for the regions of incisor, canine and premolar, the P20+ position showed the greatest difference compared to the PN. However, for the molar region, the P20- position showed the greatest difference in mean values. Conclusion: It was concluded that maxilo-mandibular positioning variations produce dimensional changes in bone height and thickness measures in ortorradial CBCT images, which may lead to errors in implants planning.
    IADR General Session 2012; 06/2012
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: To evaluate the relationship between bone height (BH) for dental implants and anterior loop (AL) of mental nerve, on right and left sides, on cone beam computed tomography (CBCT) exams. This information may be used to provide recommendations to the surgeon regarding the BH available for dental implants placement. Method: The sample consisted of 72 CBCT scans presenting AL of the mental nerve. The right and left sides were analyzed separately. In each side three BH measurements were realized: BH1 – 1mm mesial to mental foramen; BH2 – at the end of the AL; BH3 – at the middle point between BH1 and BH3. The measurements were performed by a single calibrated examiner, analyzing parasagittal reconstructions on XoranCat® software for CBCT images. Data was analyzed by paired test and simple linear regression. Result: The means of BH varied from 12.67 to 15.41 mm on the right side, and from 13.67 to 15.78 mm on the left side. Comparing the sides of the mandible, there was a statistically difference for BH1 and BH3. Simple linear regression showed that BH increases as it approaches to midline. Based on this, it was proposed two formulas: one to estimate right BH3 based on left BH1 and other to estimate right BH3 based on left BH1. Conclusion: It was concluded that right and left sides show differences in BH on the AL region and, independently of sides, BH increases as it approaches to midline, revealing the useful of the proposed formulas and ensuring safely installing of endosseous implants in the most distal area of the interforaminal region.
    IADR General Session 2012; 06/2012
  • [Show abstract] [Hide abstract]
    ABSTRACT: The aim was to evaluate the correlation between disc displacements and degenerative bone changes in magnetic resonance images (MRI) of 112 patients of both genders, with signs and symptoms of temporomandibular disorder. For this purpose, a calibrated examiner evaluated 224 MRI by assigning scores for the displacement of the disc and degenerative bone changes. Disc displacement was found in 58.42% of the temporomandibular joints (TMJs) evaluated. Anterior displacement of the disc with reduction was the most common, occurring in 67.18% cases of joints with disc displacement. Degenerative bone changes were observed in 53.94% of the TMJs analysed. There was significant correlation between disc displacement with reduction and condylar flattening, disc displacement without reduction and condylar flattening, disc displacement without reduction, and associated degenerative bone changes (flattening and erosion, flattening, osteophyte and erosion; flattening and osteophytes, erosion and sclerosis, flattening and sclerosis, flattening, osteophytes and sclerosis). The correlation between advanced cases of disc displacement and the occurrence of degenerative bone changes emphasises the importance of MRI for an accurate diagnosis and development of an appropriate treatment plan and in cases in which clinical examination is not sufficient for these purposes.
    International Journal of Oral and Maxillofacial Surgery 03/2012; 41(9):1051-7. DOI:10.1016/j.ijom.2012.03.005 · 1.36 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objectives: to evaluate, by immunohistochemistry, vimentin and osteopontin expression in the repair of bone defects created in femurs of Wistar albinus rats treated with organic bovine bone graft and associated with low level laser therapy. Materials and methods: Histological slides were obtained from blocks of different animals which were divided as follow: group I (control), group II (Gen-Ox ) and group III (LLLT and Gen-Ox ). Four slides of days 3, 5, 7 and 15 were prepared. Two slides were assessed for the expression of osteopontin and two of vimentin. Two fields were considered for analysis: one in the bone-defect interface and the other near the periosteum. To perform the immunohistochemistry anti-vimentin and anti-osteopontin, we used the classical avidin-biotin peroxidase anti-peroxidase method. The positive staining was determined by identification of intracytoplasmic brown color in the reactions with both antibodies. The sections were analyzed in Zeiss microscope at a magnification of 200x, 400x and 1000x by two different observers. The average intensity of positive cells stained in the fields in each period was determined by a semiquantitative counting method which was classified by a scoring system as follow: 0 = no marking; + = mild labeling (up to one third of positive cells); + + = moderate labeling (up to two thirds of positive cells) and + + + = intense labeling (over two thirds of labeled cells). Results: All groups had marked for vimentin and osteopontin in all periods. We observed a stronger cell labeling for vimentin in the initial healing period in group III. There were no differences in cell labeling for osteopontin in animals subjected to low level laser therapy associated with graft as compared to other groups. Conclusion: From the above results, the expression of vimentin and osteopontin during early bone repair was observed within bone tissue.
    IADR General Session 2011; 03/2011
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: This study aimed to determine the standard of gene expression of iNOS in gingival tissue from pregnancy women with and without periodontal disease. Method: Data were expressed as Cycle threshold (Ct) values and relative quantification performed. The comparison between the two groups was performed using the program Rest 2008. Gingival tissue was collected from an area with presence of periodontal pockets deeper than 4 mm in women with periodontal disease. In women without periodontal disease gingival tissue was collected from adjacent tissue to teeth with indication for extraction. All samples were collected during dental care in a School of Dentistry in Juiz de Fora, in the state of Minas Gerais, Southeast region of Brazil. Samples of gingival tissue (about 54 mg) were immediately immersed in 1.5 mL RNALatter and stored at -20 C. Total RNA was extracted using the RNeasy Mini Kit (Qiagen), following the manufacturer's recommendations. The first strand was synthesized and cDNA analysis of iNOS was performed by means of Real Time PCR. Results: This study analyzed the expression of iNOS gene into two pools consisting of samples of gingival tissue from these groups. The best concentrations of primer and the target gene cDNA for iNOS and the endogenous controls β-actin and GAPDH were 100 nM400 ng, 50 nM100 ng and 200 nM100 ng, respectively. The amplification efficiency of target gene and endogenous controls was 0.9 and the temperatures of dissociation were 79C, 86.2C and 86.5C, respectively. There were no peaks related to the amplification primer dimmer or nonspecific products to any genes when analyzing the dissociation curve. Conclusion: The pre-established conditions for the analysis will be able to draw a profile of differential expression for each group.
    03/2011
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objectives: This study was designed to address the association between maternal periodontal disease and preterm birth in a group of Brazilian women. Methods: A case-control study with post partum women aged 18-40 yrs was conducted. The mothers included those who gave birth between January and June 2010 at the obstetrics clinic of a general hospital in Juiz de Fora, Minas Gerais, Brazil. Demographic and socioeconomic data, information on current and anterior pregnancy histories, and periodontal clinical parameters were collected until 48 hours after childbirth. Periodontal disease was assessed according to two alternative definitions, namely: definition 1 four or more teeth with at least one site showing probing depth (PD)≥4 mm and clinical attachment loss (CAL)≥3 mm; and definition 2 at least one site showing PD and CAL≥4 mm. For each of these definitions, multivariate logistic regression analysis was conducted. Odds ratio (OR) was calculated with a 95% confidence interval (CI), to evaluate the association between periodontal disease and prematurity. Results: A total of 296 post partum women met the inclusion criteria and were divided as followed: case group - 74 women who delivered a preterm neonate; and control group the other 222 women. There were no significant differences neither in demographic information, except for origin municipality, nor in obstetric characteristics. Increased appetite and number of daily toothbrushing were associated with prematurity, regardless the definition of periodontal disease used. Definition 1 of periodontal disease was not associated with fewer weeks of gestation (OR=1.62; CI95%: 0.80-3.29; p=0.178). Significant association was found between definition 2 of periodontal disease and preterm birth (OR=1.98; CI95%: 1.14-3.43; p=0.015). Conclusion: The results suggest that periodontal disease may be an associated risk factor for prematurity, as well as point to the need of regular investigation on periodontal condition during pregnancy.
    IADR General Session 2011; 03/2011
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: To investigate the effect of deviation of mandibular positioning on the measured height and width of dental implant sites in cross-sectional images of cone beam computed tomography (CBCT). Methods: Three macerated mandibles, which received metallic markers in four different regions, incisors, canines, premolars and molars, were subjected to CT scans in three positions: (1) standard, (2) with an inclination above 20 and (3) with an inclination below 20. One examiner measured the bone height and width at selected sites in the images at two different times. Later, the fix tool of the XoranCat software was used to correct the images obtained with supero-inferior inclinations. The same examiner measured the bone height and width of the corrected images. The means of the variables under study, height and width, in standard-position and in lower and upper position were compared in each of the evaluated regions, for the initial and corrected images, using parametric tests in SAS 8.02. Results: There were no statistically significant differences (p> 0.05) for any of the regions studied, in bone height and thickness, when supero-inferior inclinations were used, as well as in the corrected images. However, the inclinations generated oblique orthoradial cuts relative to the images obtained without inclination, and this fact is very evident in the premolar regions, where there was variation in the images corresponding to the mandibular foramen. Conclusion: Small changes in mandibular positioning do not produce dimensional changes in the linear measurements of CBCT orthoradial images that would justify re-examination, exposing the patient to another dose of radiation.
    IADR General Session 2010; 07/2010
  • [Show abstract] [Hide abstract]
    ABSTRACT: Amalgam has been used as a filling material for over 150 years. Mercury, copper, and zinc are present in restoration. The aim of this study was to compare mercury, copper, and zinc concentrations in extracted human teeth with amalgam restorations and teeth without restorations. Thirty-two teeth, 15 restored with dental amalgam and 17 without restorations, were chemically analyzed in an Optima 3300 DV (Perkin Elmer) plasma emission spectrometer. Mercury, copper, and zinc were found in human teeth regardless of the presence of amalgam restorations. The highest mercury concentrations were found in the coronary portions of the teeth with amalgam restorations. Copper concentrations were very high. Zinc concentrations in the teeth without restoration were lower than those seen in the coronary portion of the teeth with restorations.
    Archives of Environmental and Occupational Health 11/2009; 64(4):266-9. DOI:10.1080/19338240903339955 · 0.47 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The aims of this study were to correlate the clinical and radiographic stability of titanium miniscrews when used as orthodontic anchorage for maxillary canine retraction and to assess bone quality. Thirty titanium miniscrews were placed in 15 consecutive patients (8 male, 7 female; age range, 12 years 5 months-32 years 11 months) as orthodontic anchorage. Orthodontic loads were applied immediately after miniscrew placement (T1) with a nickel-titanium closing coil spring. The initial estimated load was 200 g. The bone quality in each region of interest was determined by multi-slice computed tomography. Bone mineral density (BMD) values ranged from 167 HU to 660.80 HU (mean, 420.63 HU). The specific regions had a mean close to the maximum value of a previously established scale for the posterior region of the maxilla (0-500 HU). The paired t test showed a statistically significant difference (P = 0.450) when the means of the differences between the right and left sides were compared. Twelve of the 15 subjects had significantly greater maxillary BMD on the right side. Linear regression also showed a low correlation between the 2 sides (P = 0.097). Clinically, the success index was 100%. Although 2 miniscrews were removed from 1 patient because of severe gingival inflammation with purulent secretion, none of the 28 remaining miniscrews showed any mobility after 90 days (T2). Comparisons of the means at T1 and T2 showed no statistically significant differences in these distances: between nasion and the miniscrew head, between orbitale and the miniscrew head, and between nasion and orbitale, assessed through cephalometric tracings made on lateral oblique radiographs (45 degrees ), pointing to the stability of the 28 miniscrews during the 90-day observation period. BMD values of all subjects were within the normal range of an established scale, and even close to maximum values. The regions between the maxillary second premolars and first molars, and mesial to the maxillary second premolars, are safe as far as bone quality is concerned for miniscrew placement during the first 90 days of canine distalization. A good surgical technique and appropriate planning for miniscrew placement, inflammation control, and adequate oral hygiene are fundamental to the success of this new anchorage system during maxillary canine distalization.
    American journal of orthodontics and dentofacial orthopedics: official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics 09/2009; 136(2):243-50. DOI:10.1016/j.ajodo.2007.08.031 · 1.44 Impact Factor