L Mancinelli

Publications of L Mancinelli

• Investigation of Toxoplasma gondii presence in farmed shellfish by nested-PCR and real-time PCR fluorescent amplicon generation assay (FLAG).

  Authors: L Putignani, L Mancinelli, F Del Chierico, D Menichella, D Adlerstein, M C Angelici, M Marangi, F Berrilli, M Caffara, D A Frangipane di Regalbono, A Giangaspero

  Experimental parasitology. 10/2010; 127(2):409-17.

  To evaluate the presence of Toxoplasma gondii in edible farmed shellfish, 1734 shellfish specimens i.e., 109 Crassostrea gigas (6 pools), 660 Mytilus galloprovincialis (22 pools), 804 TapesTo evaluate the presence of Toxoplasma gondii in edible farmed shellfish, 1734 shellfish specimens i.e., 109 Crassostrea gigas (6 pools), 660 Mytilus galloprovincialis (22 pools), 804 Tapes decussatus (28 pools) and 161 Tapes philippinarum (6 pools), were collected from the Varano Lagoon (Apulia, Italy). Shellfish from 62 pools were subjected to two molecular techniques: a nested-PCR assay, and a fluorescent amplicon generation (FLAG) real-time PCR assay, both based on the multi-copy B1 target, were performed. One pooled sample of gills from C. gigas and one pooled sample of haemolymphs from T. decussatus were assessed as positive for T. gondii DNA by both techniques. The results demonstrated the presence of T. gondii in edible farmed C. gigas and T. decussatus and indicate that there may be a considerable health threat involved in eating contaminated raw shellfish.

• High Inter-Laboratory Reproducibility of MALDI-TOF Mass Spectrometry-Based Species Identification of Nonfermenting Bacteria.

  Authors: A Mellmann, F Bimet, C Bizet, A. D. Borovskaya, R. R. Drake, U Eigner, A M Fahr, Y. He, E. N. Ilina, M Kostrzewa, T Maier, L Mancinelli, W. Moussaoui, G Prévost, L Putignani, C. L. Seachord, Y. W. Tang, D Harmsen

  Journal of clinical microbiology. 09/2009;

  MALDI-TOF MS has emerged as rapid, cost-effective alternative for bacterial species identification. Identifying 60 blind-coded nonfermenting bacteria samples, this international study (eightMALDI-TOF MS has emerged as rapid, cost-effective alternative for bacterial species identification. Identifying 60 blind-coded nonfermenting bacteria samples, this international study (eight laboratories) achieved 98.75% inter-laboratory reproducibility. Only six of 480 samples were misidentified due to interchanges (4 samples) or contamination (1), or not identified because of insufficient signal intensity (1).