Publications (22)38.23 Total impact
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Article: Investigation of in vivo metabolic profile of Abelmoschus Manihot based on pattern recognition analysis.
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ABSTRACT: ETHNOPHARMACOLOGICAL RELEVANCE: Abelmoschus manihot (L.) Medik. var. manihot is one of the most commonly used Chinese medicines and has played an important role in treating chronic glomerulonephritis and diabetic nephropathy. AIM OF THE STUDY: Metabolites identification of traditional Chinese medicine (TCM) is a complex and time-consuming process due to the complicity of TCM and subsequent large number of detected ions. In this paper, UPLC-MS combined with pattern recognition analysis approach were used to simplify and quicken the identification of the metabolites of Abelmoschus Manihot. MATERIALS AND METHODS: Rat urine samples were collected before (as control sample) and after Abelmoschus Manihot administration. Pattern recognition analysis method was used to differentiate components between Abelmoschus Manihot-treated group and its controlled comparison. These components could be considered as Abelmoschus Manihot-related metabolites in vivo. RESULTS: LC-MS based metabolomics could be an advanced tool to help us find metabolites with regards to its capacity of processing large datasets, differentiating and classifying of sample groups, as well as its indiscriminative nature of biomarker and metabolite identification. Using this method, seven metabolites were identified, which are flavonoid aglycone glucuronidation, sulfatation, and methylation metabolites. CONCLUSION: Our results showed that UPLC-MS based- pattern recognition analysis approach can be used to quickly identify Abelmoschus Manihot related metabolites in biological fluids. Furthermore, this work demonstrates the potential application of combining the UPLC-MS approach with the metabolomics approach in identifying the metabolites of TCM.Journal of ethnopharmacology 04/2013; · 2.32 Impact Factor -
Article: [Study on antioxidant interaction of different preparations and proportions of danggui-chuanxiong drug pair].
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ABSTRACT: To observe the in vitro antioxidant interaction of different preparations and proportions of Danggui-Chuanxiong drug pair in the DPPH free radical scavenging rate with the response surface methodology. The 2,2-diphenyl-1-picryl-hydrazyl (DPPH) free radical scavenging rate method was adopted for determining the antioxidant activity of extracts from Danggui-Chuanxiong with 10 proportions and three extraction processes. The response surface methodology was used to determine the parameters of the dose-effect curve and establish a three-dimensional response surface model. The three-dimensional response surface graph was constructed with Matlab software. All of the 30 samples with different proportions and preparations had antioxidant effect in scavenging free radicals and a remarkable dose-effect relationship. Their water extracts had a narrow synergistic range, with only spot distribution. Their antagonist ranges were districted in six bands of various widths. The synergistic ranges of ethanol extracts were districted in small bands, with the antagonist ranges scattered in points. The synergistic ranges of their water-alcohol extracts were distributed in three bands, with their antagonist ranges scattered in points. In short, the water-alcohol extracts showed a wider synergistic range than ethanol extracts, followed by water-extracts. All of the three extraction processes showed no obvious synergistic and antagonist effects. The quantitative study on the interaction of traditional Chinese medicines with different compatibilities with the response surface methodology provides reference of thoughts and methods for relevant studies.Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 01/2013; 38(2):234-8. -
Article: Application of microdialysis for elucidating the existing form of hyperoside in rat brain: Comparison between intragastric and intraperitoneal administration.
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ABSTRACT: ETHNOPHARMACOLOGICAL RELEVANCE: Hypericum perforatum (St. John's wort) is an important anti-depressant herb used in clinic and commonly prescribed for mild depression. Hyperoside is one of the major components of H. perforatum and is also detected in many plant species such as Abelmoschus manihot, Black Currant, Rosa agrestis, Apocynum venetum and Nelumbo nucifera. Aim of the study: As the hyperoside showed CNS (central nervous system) protective activity (e.g. anti-depressant-like effect), the possibility of hyperoside or its metabolites to reach CNS should be investigated. Moreover, the pharmacokinetics profile of hyperoside or its metabolites in rat brain should be studied for further elucidating the mechanism of hyperoside action on CNS. MATERIAL AND METHODS: A simple method for simultaneous determination of unbound hyperoside and its metabolite 3'-O-methyl-hyperoside in rat brain was developed by using ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) and microdialysis technique. This method was applied for pharmacokinetics study of hyperoside and 3'-O-methyl-hyperoside in rat brain after intragastric (i.g.) and intraperitoneally (i.p.) administration of hyperoside in vivo. RESULTS: Results showed that neither hyperoside nor its metabolites were detected in rat brain after i.g. administration but both compounds could be detected after i.p. administration. Considering the activity of hyperoside through both i.g. and i.p. administration, our results imply that the active components of hyperoside in vivo might be different. Therefore, further studies are needed to identify the active components of hyperoside in vivo through these two different routes. Moreover, non-oral administration route (e.g., i.p.) should be further investigated and be explored to obtain higher bioavailability and better activity for hyperoside. Our results also showed that the real existing form of hyperoside in rat brain were hyperoside and its methylated metabolite with maximum concentration to be 63.78ng/mL and 24.66ng/mL after 20mg/kg i.p. administration, respectively. Therefore, a more reasonable concentration of hyperoside should be considered in in vitro assay to reflect the real situation of hyperoside concentration in vivo. CONCLUSION: Due to the wide use of herbal remedies containing hyperoside, our investigation will contribute to further clarifying the action of this substance. Moreover, this method will be applied for clinical pharmacokinetics study of hyperoside and its metabolite as well as herbs that contain hyperoside.Journal of ethnopharmacology 10/2012; · 2.32 Impact Factor -
Article: [Analysis on correlation between general efficacy and chemical constituents of danggui-chuanxiong herb pair based on artificial neural network].
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ABSTRACT: To evaluate the effect of danggui-chuanxiong herb pair with different prescription proportions and formulas on nourishing and tonifying blood (NTB), activating blood circulation and dissolving blood stasis (ADBS), regulating menstruation and relieving pain (RMRP) in an all-round way, in order to reveal the correlation between the effect and constituents and their degrees. First, the indexes of effect were standardized. All effects were integrated by using multi-index aggregative index method. The weight coefficients of each index were worked out by the combining the statistics of testing frequency of each effect index given by the specialist-scored method and in relevant literatures released in the latest 10 years. Then, the total effect values were obtained by multiplying the standardized value of each index with the weight coefficients and then adding their results together. Finally, a fitting analysis was made on the contents of seven major active components in danggui-chuanxiong herb pair, total aromatic acids and total phthalide lactones and their total effect by using the artificial neural network, respectively. Water extracts of danggui-chuanxiong (1.5:1) had the best effect on total NTB; alcohol extracts of danggui-chuanxiong herb pair (1:1) had the best effect on total ADBS; alcohol extraction of danggui-chuanxiong herb pair (1.5:1) had the best effect on RMRP. By using the same extraction method, extracts of danggui had better effect on total NTB than Chuanxiong; while extracts of chuanxiong had better effect on total ADBS and RMRP than danggui. With the same prescription proportion, water extracts of danggui-chuanxiong herb pair had the best effect on total NTB; alcohol extracts of danggui-chuanxiong herb pair had the best effect on total ADBS and RMRP. Aromatic acids were the main effective components for NTB, especially chlorogenic acid and caffeic acid. Both aromatic acids and phthalide lactones were active components for ADBS, particular chlorogenic acid and senkyunolides I and H showed greater effect on ADBS. Both aromatic acids and phthalide lactones were also the important active constituents for the effect of RMRP, especially ligustilide, caffeic acid, ferulic acid, and senkyunolide I showed greater effect on RMRP. Both aromatic acids and phthalide lactones of danggui-chuanxiong herb pair contributed to NTB, ADBS and RMRP. Aromatic acids laid particular stress on NTB and ADBS, while phthalide lactones laid particular stress on RMRP. In this study, determined effect indexes of the herbal medicines were integrated by using multi-index aggregative index method, and a fitting correlation analysis was made on the component content of the herbal medicines and their integration effect by using the artificial neural network, in order to specify the active constituents of the herbal medicines with different effects and their contributions to the general efficacy and provide new ideas and methods for basic study on complicated active constituents of the herbs medicines.Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica 10/2012; 37(19):2935-42. -
Article: [Interaction of nourishing and tonifying blood effects of the combination of Angelicae sinensis Radix and Astragali Radix studied by response surface method].
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ABSTRACT: The combination of Angelicae sinensis Radix (Danggui, DG) and Astragali Radix (Huangqi, HQ) is a popular herb pair commonly used in clinic for the treatment of blood deficiency syndrome in China. The aim of this paper is to study the interaction of DG and HQ nourishing and tonifying blood effects by response surface method. The blood deficiency mice were induced by injecting N-acetylphenylhydrazine (sc) and cyclophosphamide (ip). The blood deficiency mice were administrated intragastrically with DG-HQ extracts (0:1, 1: 5, 2:5, 2:3, 1:1, 3:2, 5:2, 5:1, 1:0). The changes of the peripheral blood indexes and organ indexes were observed. The indexes were integrated by comprehensive index method; the interactions of DG and HQ were analyzed by the response surface diagram established with Matlab software. The results showed that DG and HQ at most of their combination ratios had synergic effect. Within the range of 1:5 - 5:1, all of the extracts of DG-HQ showed synergic effect, and among which, high-doses had better effects than low-doses. The highest value (-1) of the synergic effect was showed when DG was 10 - 40 g at the same time of HQ as 90 -180 g, and DG was 50 - 100 g at the same time of HQ as 20 - 100 g. DG-HQ at all combination dosages within Chinese Pharmacopeia (DG: 6 - 12 g, HQ: 9 - 30 g) had certain synergic effect, and Danggui Buxue Decoction (DG: 6 g, HQ: 30 g) also was at this range. The results provided scientific basis to the clinical application of DG and HQ. And the response surface method was firstly applied to quantitatively evaluate the bio-activity change of herb combination, which provided a novel way for modern basic research on the interaction of herbs.Yao xue xue bao = Acta pharmaceutica Sinica 10/2012; 47(10):1375-83. -
Article: [The incompatibility mechanism based on the interaction of multiple-components for Flos Genkwa and Radix et Rhizoma Glycyrrhizae].
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ABSTRACT: By investigating the interaction between components from Flos Genkwa (FG) and Radix et Rhizoma Glycyrrhizae (RRG) and the dissolution profile of toxic components in co-decoction, the characteristics and possible mechanism of incompatibility were revealed. Ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) and ultra-high performance liquid chromatography triple-quadrupole mass spectrometry (UPLC-TQ/MS) were used to analyze multi-components in different herb extractions prepared by different ratios of FG/FG processed by vinegar (FGV) and RRG, which reflect the interaction and characteristics of multiple components in incompatibility combinations. The results showed that the components dissolution was influenced by compatibility ratio with certain regularity. Whether FG processed by vinegar or not, with the increase of RRG in co-decoction, the dissolution of diterpenes, especially for yuanhuacine, yuanhuadine and yuanhuajine, the toxic ingredients of FG, increased significantly. From these results, the material basis and one possible mechanism of incompatibility between FG and RRG is the increasing dissolution of diterpenes, toxic components of FG in co-decoction process, which caused by interaction between multi-components in these two herbs.Yao xue xue bao = Acta pharmaceutica Sinica 08/2012; 47(8):1043-8. -
Article: [Comparative assessing the effects of angelica root and chuanxiong on the hemorheology and the blood coagulation function in acute blood stasis rats].
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ABSTRACT: To comparatively assess the effects of angelica root and chuanxiong on the hemorheology and the blood coagulation function in acute blood stasis rats. Ice water bath and subcutaneous injection of adrenaline were both used to establish the acute blood stasis rat model. The whole blood viscosity, plasma viscosity, erythrocyte sedimentation rate (ESR), and haematocrit were tested to observe the effects of angelica root and chuanxiong on the hemorheology of blood stasis rats. The prothrombin time (PT), activated partial thromboplastin time (APTT), and plasma fibrinogen (FIB) were tested to observe the effects of angelica root and chuanxiong on the blood coagulation function of blood stasis rats. Finally all those indices were integrated to the total activating blood circulation effect value to assess the total effects of angelica root and chuanxiong on activating blood circulation and dissipating blood stasis. Compared with the normal control group, the hemorheological indices obviously increased, PT and APTT were obviously shortened, and the FIB content obviously increased in the model group. Compared with the model group, the water extracts, alcohol extracts, water-alcohol extracts of angelica root, chuanxiong, angelica root and chuanxiong 1:1, and angelica root and chuanxiong 1.5:1 could decrease the hemorheological indices, prolong the PT and APTT, and significantly reduce the content of FIB. Under the condition of the same preparation method, the alcohol extract of angelica root and chuanxiong 1:1 showed the best total effect. As for the single herbs, chuanxiong had better effect than angelica root on the total effects of activating blood circulation and dissipating blood stasis. Under the condition of the same ratio, the alcohol extracts of angelica root and chuanxiong showed better total effects of activating blood circulation and dissipating blood stasis than water extracts and water-alcohol extracts. The angelica root and chuanxiong could obviously improve the abnormality of hemorheology and blood coagulation function in acute blood stasis rats, thus providing evidence for revealing the scientific innovation of the compatibility of angelica root and chuanxiong.Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban 06/2012; 32(6):806-11. -
Article: [Absorption of flavonoids from Abelmoschus manihot extract by in situ intestinal perfusion].
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ABSTRACT: To explore the mechanism of the absorption of flavonoids from Abelmoschus manihot flowers, in situ intestinal recirculation was performed to study the effect of the absorption at different concentrations and different intestinal regions. To evaluate the conditions of the absorption of six flavonoids from Abelmoschus manihot flowers, the concentrations of Abelmoschus manihot in the perfusion solution were determined by HPLC at predesigned time. And we have investigated the inhibitory effect of six flavonoids from Abelmoschus manihot flowers on P-glycoprotein (P-gp) drug efflux pump. The results demonstrated that the absorption rates of flavonoids from Abelmoschus manihot flowers are not significantly different (P > 0.05) at various drug concentrations, the absorption of flavonoids from Abelmoschus manihot flowers is a first-order process with the passive diffusion mechanism. The absorption rates of each of flavonoids are significantly different. The absorption rate of flavonoid glycoside was lower than that of aglycone; the flavonoids from Abelmoschus manihot flowers could be absorbed in all of the intestinal segments. The best parts of intestine to absorb hyperoside and myricetin are jejunum and duodenum, separately. Verapamil could enhance the absorption of isoquercitrin, hyperoside, myricetin and quercetin-3'-O-glucoside by inhibiting P-glycoprotein (P-gp) drug efflux pump.Yao xue xue bao = Acta pharmaceutica Sinica 04/2011; 46(4):454-9. -
Article: Synthesis and bio-activity evaluation of scutellarein as a potent agent for the therapy of ischemic cerebrovascular disease.
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ABSTRACT: Scutellarein, the main metabolite of scutellarin in vivo, has relatively better solubility, bioavailability and bio-activity than scutellarin. However, it is very difficult to obtain scutellarein in nature compared with scutellarin. Therefore, the present study focused on establishing an efficient route for the synthesis of scutellarein by hydrolyzing scutellarin. The in vitro antioxidant activities of scutellarein were evaluated by measuring its scavenging capacities toward DPPH, ABTS(+•), (•)OH free radicals and its protective effect on H(2)O(2)-induced cytotoxicity in PC12 cells using MTT assay method. The results showed that essential point to the synthesis was the implementation of H(2)SO(4) in 90% ethanol in N(2) atmosphere; scutellarein had stronger antioxidant activity than scutellarin. The results have laid the foundation for further research and the development of scutellarein as a promising candidate for ischemic cerebrovascular disease.International Journal of Molecular Sciences 01/2011; 12(11):8208-16. · 2.60 Impact Factor -
Article: Qualitative and quantitative analysis of the major bioactive phenolic compounds of Glechoma longituba by LC-coupled with PAD and ESI-MS detection.
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ABSTRACT: A sensitive and selective method based on HPLC-PAD and Quadrupole TOF ESI-MS-MS is presented for quality control of Glechoma longituba, the major bioactive phenolic compounds of which could be quantified simultaneously. LC-ESI-MS was applied for nine fully identified compounds and another eight partially identified compounds in the plant by comparing their mass spectral data and retention times with those of selected standards and literature data. For quantitative analysis, the chromatographic conditions and extraction procedure were optimized in order to ensure the exhaustive extraction of the plant material. The calibration curves for determination of the nine compounds showed good linearity over the tested ranges (r2 > 0.999). Measurement of intra-day and inter-day variability was conducted to assess precision of the method, and their RSDs were between 2.22%-2.92% and 1.35%-2.95%. The RSDs of repeatability and stability were between 0.90%-2.53% and 1.73%-2.94%. The recoveries of the nine compounds were between 94.7%-106.5%, with RSD value ranging from 0.56%-2.88%. These validation results demonstrated the suitability of the method for the precise and accurate determination of the main constituents of G. longituba. The method was used for the quality evaluation of twenty batches of G. longituba obtained from different regions of China. The multi-index comprehensive evaluation method for the different kinds of compounds can ensure the efficacy and safety of the traditional Chinese medicine.Natural product communications 01/2011; 6(1):17-20. · 1.24 Impact Factor -
Article: The protective effects of the active fraction of Shaofu Zhuyu decoction on hydrogen peroxide-induced oxidative injury in vascular smooth muscle cells.
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ABSTRACT: In this paper, the protective effects of the active fraction (SF-7) from Shaofu Zhuyu decoction (SFZYD) were tested on a hydrogen peroxide (H(2)O(2))-induced rat vascular smooth muscle cells (VSMCs) oxidative injury model. This active fraction (SF-7) shows potent antioxidant properties. The cell viability and oxidative damage markers of VSMCs were determined after exposure to H(2)O(2) for 16 hours. It was observed that SF-7 significantly increased cell survival and reduced apoptosis of H(2)O(2)-injured VSMCs. Moreover, SF-7 could markedly increase intracellular superoxide dismutase (SOD) activity and decrease the malondialdehyde (MDA) level in H(2)O(2)-injured VSMCs, and suppress the generation of intracellular reactive oxygen species (ROS), as well as intracellular Ca2+ concentration. Thus, SF-7 exhibits protective effects against H(2)O(2)-injury on VSMCs, which may be associated with its antioxidant properties. It is suggested that SF may be useful in the treatment of blood stasis syndrome in which oxidative injury is mainly implicated.Molecules 08/2010; 15(8):5066-78. · 2.39 Impact Factor -
Article: Purification and identification of three novel antioxidant peptides from Cornu Bubali (water buffalo horn).
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ABSTRACT: Cornu Bubali (water buffalo horn, WBH) has been used in Traditional Chinese Medicine (TCM) for thousands of years. In the present study, three peptides with antioxidant properties were purified from aqueous extract of Cornu Bubali (water buffalo horn, WBH) by consecutive chromatographic methods including gel filtration chromatography, ion-exchange chromatography and high performance liquid chromatography. The sequences of the three peptides were identified to be Gln-Tyr-Asp-Gln-Gly-Val (WBH-1, 708Da), Tyr-Glu-Asp-Cys-Thr-Asp-Cys-Gly-Asn (WBH-2, 1018Da) and Ala-Ala-Asp-Asn-Ala-Asn-Glu-Leu-Phe-Pro-Pro-Asn (WBH-3, 1271Da) by matrix assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry (MALDI-LIFT-TOF/TOF MS). The antioxidant activity of these peptides was tested using 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging assay directly. Methylthiazol tetrazolium (MTT) assay and lactate dehydrogenase (LDH) release assay were also used to evaluate the protection of peptides against hydrogen peroxide (H(2)O(2)) induced injury. The results showed that these peptides could reduce the DPPH radical and protect rat cerebral microvascular endothelial cells (rCMECs) against H(2)O(2)-induced injury, thus demonstrating that these peptides had antioxidant activity. These results suggest that WBH-1, WBH-2 and WBH-3, isolated from the aqueous extract of water buffalo horn are natural antioxidants and may contribute to the efficacy of WBH.Peptides 03/2010; 31(5):786-93. · 2.43 Impact Factor -
Article: Characterization of the active constituents in Shixiao San using bioactivity evaluation followed by UPLC-QTOF and Markerlynx analysis.
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ABSTRACT: Shixiao San is a famous Traditional Chinese Medicine (TCM) formula that has been used for a long time for the treatment of gynecological diseases. In this paper, the active constituents in Shixiao San were characterized by using bioactivity evaluation followed by UPLC-QTOF and Markerlynx analysis. The analgesic activities of two extracts of Shixiao San were evaluated using the hot-plate test, acetic acid-induced writhing and dysmenorrhea mice model. The results showed that the analgesic activity of Shixiao San vinegary extract (boiling vinegar) was superior to the aqueous extract. UPLC-QTOF and Markerlynx analytic results showed that the process of boiling in vinegar may improve the dissolution of Shixiao San flavanoids, and these flavanoids may contribute to the observed analgesic activity. This work demonstrated that UPLC/QTOFMS and Markerlynx could serve as new methods for fast generation and automated analysis of information-rich data from Chinese herbal medicines.Molecules 01/2010; 15(9):6217-30. · 2.39 Impact Factor -
Article: Four new fatty acid esters from the Feces of Trogopterus xanthipes.
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ABSTRACT: Four new fatty acid esters have been isolated from Feces Trogopterus. Their structures were determined by spectroscopic and chemical methods to be bis(7-hydroxyheptyl) icosanedioate (1), bis(7-hydroxyheptyl) heptadecanedioate (2), bis(7-hydroxyheptyl) decanedioate (3), and bis(7-hydroxyheptyl) octanedioate (4). In the anticoagulative assay, compounds 3 and 4 had significant antithrombin activity.Lipids 09/2009; 44(9):849-53. · 2.13 Impact Factor -
Article: [Chinese herbal medicine PC-SPES II induces the apoptosis of androgen-independent prostate carcinoma cell line PC-3].
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ABSTRACT: To study the action mechanism of a Chinese herbal mixture PC-SPES II inducing the apoptosis of androgen independent prostate adenocarcinoma cell line (PC-3). The growth of PC-3 was shown by MTT. Immunofluorescence staining of acridine orange (AO) and flow cytometry were used to detect the apoptosis. The expressions of the apoptosis-related proteins were analyzed with their monoclonal or polyclonal antibodies after Western blotting. PC-SPES II not only inhibited the growth of PC-3 cells but also induced their death. The apoptosis of PC-3 cells treated with PC-SPES II was detected by immunofluorescence staining of AO and flow cytometry, which showed the apoptotic cells to be (29.8 +/- 5.6)%, but the untreated control cells (0.06 +/- 0.014)%, (P < 0.01). The expression of Bcl-2 and Bcl-xL, two antiapoptosis proteins, was decreased while Bax, a pro apoptosis protein, was elevated in the cells treated with PC-SPES II as compared with the untreated control (P < 0.01). Accordingly, the expression of the activated fragments of caspase-3 was also increased (P < 0.01). The Chinese herbal mixture PCSPES II can induce the apoptosis of PC-3. Its mechanism may lie in the up-regulation of Bax expression and down-regulation of Bcl-2 and Bcl-xL expressions, which induce the activated fragments of caspase-3 by mitochondria.Zhonghua nan ke xue = National journal of andrology 07/2007; 13(6):563-7. -
Article: Characterization of a novel human UDP-GalNAc transferase, pp-GalNAc-T15.
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ABSTRACT: We have cloned, expressed and characterized a novel member of the human UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) family, pp-GalNAc-T15. The pp-GalNAc-T15 transcript was ubiquitously expressed in human tissues. Recombinant pp-GalNAc-T15 transferred N-acetylgalactosamine (GalNAc) toward a panel of mucin-derived peptide substrates in vitro. Although pp-GalNAc-T15 showed significantly less catalytic activity than pp-GalNAc-T2, T15 transferred up to seven GalNAcs to the Muc5AC peptide, while T2 transferred up to five GalNAcs. These results clearly indicated that pp-GalNAc-T15 is a novel member of the human pp-GalNAc-T family with unique catalytic activity.FEBS Letters 06/2004; 566(1-3):17-24. · 3.54 Impact Factor -
Article: Expression of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-12 in gastric and colonic cancer cell lines and in human colorectal cancer.
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ABSTRACT: The expression of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-12 (pp-GalNAc-T12) was studied in 3 normal human tissues (stomach, small intestine and colon), 3 stomach and 6 colon cancer cell lines, as well as in the resected cancer tissues and normal tissues (control) from 19 patients with colorectal cancer. Marathon Ready cDNAs were used as the templates of normal tissues. mRNA was extracted from the cell lines and resected tissues, and reverse-transcribed to cDNA. The expression of pp-GalNAc-T12 was determined with a real-time polymerase chain reaction (PCR). It was found that the expression of pp-GalNAc-T12 was strong in 3 normal tissues, weak or negligible in 9 cancer cell lines, and down-regulated in all of the colorectal cancer tissues as compared with normal control samples. Moreover, the expression of pp-GalNAc-T12 tended to inversely correlate with the TNM stage, and statistically was much lower in the samples with metastasis than in those without. However, the expression in the tissues did not correlate with the concentration of serum CA 19-9 routinely applied in the diagnosis and assessment of prognosis in patients with colonic cancers. the expression of pp-GalNAc-T12 seems to be a negative marker especially of metastatic gastric and colorectal cancer.Oncology 02/2004; 67(3-4):271-6. · 2.27 Impact Factor -
Article: Cloning and characterization of a novel UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase, pp-GalNAc-T14.
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ABSTRACT: A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) gene family was cloned and designated pp-GalNAc-T14. This type II membrane protein contains all motifs that are conserved in the pp-GalNAc-T family proteins and forms a subfamily with pp-GalNAc-T2 on the phylogenetic tree. Quantitative real time PCR analysis revealed significantly high expression of the pp-GalNAc-T14 transcript in kidney, although the transcripts were ubiquitously expressed in all tissues examined. Furthermore, the recombinant pp-GalNAc-T14 transferred GalNAc to a panel of mucin-derived peptide substrates such as Muc2, Muc5AC, Muc7, and Muc13 (-58). Our results provide evidence that pp-GalNAc-T14 is a new member of the pp-GalNAc-T family and suggest that pp-GalNAc-T14 may be involved in the O-glycosylation in kidney.Biochemical and Biophysical Research Communications 02/2003; 300(3):738-44. · 2.48 Impact Factor -
Article: Characterization of a novel human UDP-GalNAc transferase, pp-GalNAc-T10.
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ABSTRACT: A novel member of the human UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T) gene family was cloned as a homolog of human pp-GalNAc-T7, and designated pp-GalNAc-T10. pp-GalNAc-T10 transcript was found in the small intestine, stomach, pancreas, ovary, thyroid gland and spleen. In a polypeptide GalNAc-transfer assay, recombinant pp-GalNAc-T10 transferred GalNAc onto a panel of mucin-derived peptide substrates. Furthermore, pp-GalNAc-T10 demonstrated strong transferase activity with glycopeptide substrates.FEBS Letters 12/2002; 531(2):115-21. · 3.54 Impact Factor -
Article: Molecular cloning and characterization of a novel member of the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase family, pp-GalNAc-T12.
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ABSTRACT: We cloned in silico a novel human UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase (pp-GalNAc-T), pp-GalNAc-T12. The deduced amino acid sequence of pp-GalNAc-T12 contains all conserved motifs in pp-GalNAc-T family proteins. Quantitative real time polymerase chain reaction analysis revealed that the pp-GalNAc-T12 transcript was expressed mainly in digestive organs such as stomach, small intestine and colon. The recombinant pp-GalNAc-T12 transferred GalNAc to the mucin-derived peptides such as the Muc1a, Muc5AC, EA2 peptides and the GalNAc-Muc5AC glycopeptide. Since mucins are glycoproteins mainly produced in the digestive organs, our results suggest that pp-GalNAc-T12 plays an important role in the initial step of mucin-type oligosaccharide biosynthesis in digestive organs.FEBS Letters 08/2002; 524(1-3):211-8. · 3.54 Impact Factor
Top co-authors
Top Journals
Institutions
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2012
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Nanjing University of Traditional Chinese Medicine
- Jiangsu Key Laboratory for TCM Formulae Research
Nanjing, Jiangsu Sheng, China
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2011–2012
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Nanjing Medical University
Nanjing, Jiangsu Sheng, China
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2007
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St. Luke's Hospital
Cedar Rapids, IA, USA
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2002–2004
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National Institute of Advanced Industrial Science and Technology
- Research Center for Medical Glycoscience
Ibaraki, Osaka-fu, Japan
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