Huifang Hao

Publications (2)7.03 Total impact

• Source

  Available from: PubMed Central

  Article: Porcine FcγRIIb mediates enhancement of porcine reproductive and respiratory syndrome virus (PRRSV) infection.

  Songlin Qiao, Zhizheng Jiang, Xiaohui Tian, Rui Wang, Guangxu Xing, Bo Wan, Dengke Bao, Yonghui Liu, Huifang Hao, Junqing Guo, Gaiping Zhang
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  ABSTRACT: Antibody-dependent enhancement (ADE) of virus infection caused by the uptake of virus-antibody complexes by FcγRs is a significant obstacle to the development of effective vaccines to control certain human and animal viral diseases. The activation FcγRs, including FcγRI and FcγRIIa have been shown to mediate ADE infection of virus. In the present paper, we showed that pocine FcγRIIb, an inhibitory FcγR, mediates ADE of PRRSV infection. Stable Marc-145 cell lines expressing poFcγRIIb (Marc-poFcγRII) were established. The relative yield of progeny virus was significantly increased in the presence of sub-neutralization anti-PRRSV antibody. The Fab fragment and normal porcine sera had no effect. Anti-poFcγRII antibody inhibited the enhancement of infection when cells were infected in the presence of anti-PRRSV antibody, but not when cells were infected in the absence of antibody. These results indicate that enhancement of infection in these cells by anti-PRRSV virus antibody is FcγRII-mediated. Identification of the inhibitory FcγR mediating ADE infection should expand our understanding of the mechanisms of pathogenesis for a broad range of infectious diseases and may open many approaches for improvements to the treatment and prevention of such diseases.
  PLoS ONE 01/2011; 6(12):e28721. · 4.09 Impact Factor
• Article: Characterization and ligand specificity of sheep IgG2 receptor.

  Songlin Qiao, Yanyan Yang, Yunchao Liu, Gaiping Zhang, Jun Xi, Hong Zhang, Huifang Hao, Xiaohui Tian, Suzhen Yang, Ruiguang Deng
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  ABSTRACT: Neutrophils and macrophages in cattle express a novel class of immunoglobulin Fc receptor, specific for bovine IgG2, termed boFcgamma2R. In cows, the ability of neutrophils to kill immunoglobulin-opsonized microorganisms appears to depend largely on this subclass. Although related to other mammalian FcgammaRs, boFcgamma2R belongs to a novel gene family that includes the human killer Ig-like receptor and FcalphaRI (CD89) proteins. In this study, we describe the presence and characterization of this novel class of FcgammaR in sheep. The comparative analysis of this novel FcgammaR has allowed us to begin an exploration of some immunological characteristic of ruminants.
  Immunogenetics 09/2009; 61(8):597-601. · 2.93 Impact Factor