Xiao-Li Liu

Yunnan University, Yün-nan, Yunnan, China

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Publications (5)3.25 Total impact

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    ABSTRACT: Cytoplasmic male sterility (CMS) in plants is known to be associated with structural change and the presence of new chimeric genes in mitochondrial DNA (mtDNA). In this study, the atp6 and apt9 gene fragments were cloned from mtDNA of a CMS line and its maintainer and restorer lines of ramie (Boehmeria nivea (L.) Gaud.) using PCR and degenerate primers that were designed according to the conserved sequences within the coding region of chondriogenes atp6 and atp9 of some dicotyledons recorded in GenBank. In spite of incompleteness of the coding region, the cloned fragments showed a homology of over 94% and over 85% with atp6 and atp9 genes, respectively, from the dicotyledons in GenBank. The whole sequences of atp6 and atp9 genes including the complete open reading frames were cloned by amplifying the 3′ and 5′ end unknown sequences of these gene fragments using a DNA Walking strategy. The atp6 gene showed no difference among the CMS line, maintainer and restorer lines of ramie in DNA sequence, transcription and translation, and in the levels of protein. However, compared to the atp9 gene from the maintainer and restorer lines, within the coding region, the atp9 gene from the CMS line had a number of different nucleotides and a sequence deficiency of as many as 21 nucleotides at the 3′ end. An unusual high expression of the atp9 gene in the CMS line at the budding and full-bloom stages was revealed by RT-PCR analysis. The results indicated that the variation in DNA sequence and its encoding product, and/or the abnormal expression of the atp9 gene in the CMS line, may be closely related to male sterility in ramie. This work provides basic knowledge for understanding the function of the atp9 gene causing CMS in ramie and subsequently the molecular genetic mechanisms.
    Molecular Breeding 01/2012; · 3.25 Impact Factor
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    ABSTRACT: Neopicrorhiza scrophulariiflora Pennel (Hong) (Scrophulariaceae) is an endangered medicinal plant, endemic to the Eastern Himalayas and the Hengduan Mountains. Levels of genetic variation and genetic structure of seven populations of N. scrophulariiflora in China were studied using inter-simple sequence repeat (ISSR) markers. Thirteen primers amplified 82 total loci from 7 populations composed of 136 individuals. All 82 loci were polymorphic, showing a percentage of polymorphic bands (PPB) of 100%, indicating considerable genetic variation at the species level. In contrast, a low level of genetic diversity within populations was detected with a mean PPB of 30.56%. In addition, Nei’s genetic diversity analysis (0.4073) and Shannon’s diversity index (0.5917) revealed similar genetic structure. High levels of genetic differentiation (Gst = 0.6955) and restricted gene flow (Nm = 0.2198) among populations were also detected. Anthropologic impacts, together with clonal propagation, genetic drift and geographical isolation might be the reasons which had shaped the genetic structure of this species.
    Biochemical Systematics and Ecology - BIOCHEM SYST ECOL. 01/2011; 39(4):297-301.
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    ABSTRACT: To study the difference of the quantitative multistate character of infructescence of Amomum tsao-ko from five populations, and the correlations between the quantitative multistate character and altitude, longitude and latitude. By observing and analyzing the quantitative multistate character of infructescence of Amomum tsao-ko from five populations. There is highly significant difference among the number of flowerlets each inflorescence from five populations. The higher the altitude, the less the number of flowerlets each inflorescence; and the higher the longitude is, the more the number of flowerlets each inflorescence. The temperature and the humidity of the circumstance affects the number of flowerlets each inflorescence. Lower temperature, higher diurnal variation of temperature, and higher diurnal variation of relative humidity causes less number of flowerlets each inflorescence. There is significantly negative correlation between the number of flowerlets each inflorescence and the maturing rate of infructescence. The difference of the maturing rate of infructescence from five populations don't approach significant level. The difference of the maturing rate of infructescence approaches significant level by multiple comparisons between Magnan population and Baoshan population, Gongshan population and Xichou population. There is not significant correlation between the maturing rate of infructescence and the altitude, the longitude and the latitude. The factors of the circumstance affects the number of flowerlets each inflorescence. The climate by the change of the altitude affects the number of flowerlets each inflorescence highly significantly. Selecting the fine genetic resources of Amomum tsao-ko for high yielding, the number of flowerlets of inflorescence is not better character. Maybe the difference of the maturing rate of inflorescence is mainly caused by different genetic resources. Analyzing the difference of genetic regenetic resources of Amomum tsao-ko.
    Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials 07/2010; 33(7):1034-8.
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    ABSTRACT: To study the anatomical structure of endangered alpine medical plant Neopicrorhiza scrophulariiflora and the high altitude adaptability. The leaf epidermis character as well as section structure of leaf, aerial stem and rhizome were observed by light microscopical technique. The leaf surface of Neopicrorhiza scrophulariiflora was covered with two kinds of glandular hair, and the stommata was anomocytic type. Moreover, the leaf was isolateral and differed from most of alpine plant. The aerial stem had well-developed mechanical tissue. The rhizome was distributed by well-developed cork layers and collenchyma. Large numbers of aerenchymas distributed widely in leaf, aerial stem and rhizome. There existed characteristic traits in Neopicrorhiza scrophulariiflora that adapted the alpine environment, however, there still had some particular character different from other alpine plant. Thus, the adaptive style of alpine plant to high altitude environment was diversity.
    Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials 04/2010; 33(4):507-10.
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    ABSTRACT: To explore the regulation of the growth of fruit of Amomum tsaoko and the factors influencing the weight of fruit. To compare and analyze the quantitative multistate character of fruit and seed of Amomum tsaoko. The quantitative multistate character of fruit and seed of Amomum tsaoko in populations and between populations were abundant. There was positive relativity between the rate of fertilization of ovule and the rate of fructify and this indicated the different heredity character among plants. The weight of fruit was mainly decided by the weight of seed regiment, The more the seed quantity, the larger the weight of seed regiment, and the larger the weight of fruit, then the higher the yield. The variety of the weight of fruit and the quantity of seed presented peak form along with the proper order of fruit growth in inflorescence but had no relativity. The abundant quantitative multistate character of fruit and seed of Amomum tsaoko provide the abundant materials for selecting good varieties. For high yield we should not only choose plants with high rate of fructify and high rate of fertilization of ovule the, but also pay attention to the influence of pollination to yield.
    Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials 08/2009; 32(8):1184-7.

Publication Stats

3 Citations
3.25 Total Impact Points

Institutions

  • 2011–2012
    • Yunnan University
      Yün-nan, Yunnan, China
  • 2010
    • Gansu College of Traditional Chinese Medicine
      Kan-shui, Chongqing Shi, China
  • 2009
    • Kunming University of Science and Technology
      Yün-nan, Yunnan, China