Tamara J Graves

Publications (2)2.41 Total impact

• Article: Fine mapping and expression of candidate genes within the chromosome 10 QTL region of the high and low alcohol-drinking rats.

  Paula J Bice, Tiebing Liang, Lili Zhang, Tamara J Graves, Lucinda G Carr, Dongbing Lai, Mark W Kimpel, Tatiana Foroud
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  ABSTRACT: The high and low alcohol-drinking (HAD and LAD) rats were selectively bred for differences in alcohol intake. The HAD/LAD rats originated from the N/Nih heterogeneous stock developed from intercrossing eight inbred rat strains. The HAD×LAD F2 were genotyped, and a powerful analytical approach, using ancestral recombination and F2 recombination, was used to narrow a quantitative trait loci (QTL) for alcohol drinking to a 2-cM region on distal chromosome 10 that was in common in the HAD1/LAD1 and HAD2/LAD2 analyses. Quantitative real-time PCR was used to examine mRNA expression of six candidate genes (Crebbp, Trap1, Gnptg, Clcn7, Fahd1, and Mapk8ip3) located within the narrowed QTL region in the HAD1/LAD1 rats. Expression was examined in five brain regions, including the nucleus accumbens, amygdala, caudate putamen, hippocampus, and prefrontal cortex. All six genes showed differential expression in at least one brain region. Of the genes tested in this study, Crebbp and Mapk8ip3 may be the most promising candidates with regard to alcohol drinking.
  Alcohol (Fayetteville, N.Y.) 09/2010; 44(6):477-85. · 2.41 Impact Factor
• Article: Identification of a novel cytosolic aldehyde dehydrogenase allele, ALDH1A1*4.

  Shelley M Moore, Tiebing Liang, Tamara J Graves, Kevin M McCall, Lucinda G Carr, Cindy L Ehlers
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  ABSTRACT: This paper reports the identification of a novel cytosolic aldehyde dehydrogenase 1 ( ALDH1A1 ) allele. One hundred and sixty-two Indo-Trinidadian and 85 Afro-Trinidadian individuals were genotyped. A novel ALDH1A1 allele, ALDH1A1*4 , was identified in an Indo-Trinidadian alcoholic with an A inserted at position -554 relative to the translational start site, +1. It was concluded that a wider cross-section of individuals needs to be evaluated in order to determine the representative frequency of the allele, and to see if it is associated with risk of alcoholism.
  Human genomics 08/2009; 3(4):304-7.