Publications (9)25.21 Total impact
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Article: Fibronectin potentiates topical erythropoietin-induced wound repair in diabetic mice.
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ABSTRACT: Diabetes mellitus disrupts all phases of the wound repair cascade and leads to development of chronic wounds. We previously showed that topical erythropoietin (EPO) can promote wound repair in diabetic rats. Fibronectin (FN) has a critical role throughout the process of wound healing, yet it is deficient in wound tissues of diabetic patients. Therefore, we investigated the effect of topical treatment of both EPO and FN (EPO/FN) on wound repair in diabetic mice. Full-thickness excisional skin wounds in diabetic and nondiabetic mice were treated with a cream containing vehicle, EPO, FN, or EPO/FN. We assessed the rate of wound closure, angiogenesis, apoptosis, and expression of inflammatory cytokines, endothelial nitric oxide synthase (eNOS) and β1-integrin, in the wound tissues. We also investigated the effect of EPO, FN, and EPO/FN on human dermal microvascular endothelial cells and fibroblasts cultured on fibrin-coated plates, or in high glucose concentrations. EPO/FN treatment significantly increased the rate of wound closure and this effect was associated with increased angiogenesis, increased eNOS and β1-integrin expression, and reduced expression of inflammatory cytokines and apoptosis. Our findings show that EPO and FN have an additive effect on wound repair in diabetic mice.Journal of Investigative Dermatology 02/2011; 131(6):1365-74. · 6.31 Impact Factor -
Article: Erythropoietin improves the survival of fat tissue after its transplantation in nude mice.
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ABSTRACT: Autologous transplanted fat has a high resorption rate, providing a clinical challenge for the means to reduce it. Erythropoietin (EPO) has non-hematopoietic targets, and we hypothesized that EPO may improve long-term fat graft survival because it has both pro-angiogenic and anti-apoptotic properties. We aimed to determine the effect of EPO on the survival of human fat tissue after its transplantation in nude mice. Human fat tissue was injected subcutaneously into immunologically-compromised nude mice, and the grafts were then treated with either 20 IU or 100 IU EPO. At the end of the 15-week study period, the extent of angiogenesis, apoptosis, and histology were assessed in the fat grafts. The results were compared to vascular endothelial growth factor (VEGF)-treated and phosphate-buffered saline (PBS)-treated fat grafts. The weight and volume of the EPO-treated grafts were higher than those of the PBS-treated grafts, whose weights and volumes were not different from those of the VEGF-treated grafts. EPO treatment also increased the expression of angiogenic factors and microvascular density, and reduced inflammation and apoptosis in a dose-dependent manner in the fat grafts. Our data suggest that stimulation of angiogenesis by a cluster of angiogenic factors and decreased fat cell apoptosis account for potential mechanisms that underlie the improved long-term survival of fat transplants following EPO treatment.PLoS ONE 01/2010; 5(11):e13986. · 4.09 Impact Factor -
Article: Topical erythropoietin promotes wound repair in diabetic rats.
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ABSTRACT: Wound healing in diabetic patients is slower than in healthy individuals. Erythropoietin (EPO) has non-hemopoietic targets in the skin, and systemically administered EPO promotes wound healing in experimental animals. This study investigated the effect of topical EPO treatment on defective wound repair in the skin of diabetic rats. Full-thickness excisional skin wounds were made in 38 rats, of which 30 had diabetes. The wounds were then treated topically with a cream that contained either vehicle, 600 IU ml(-1) EPO (low dose), or 3,000 IU ml(-1) (high dose) EPO. We assessed the rate of wound closure during the 12-day treatment period, and microvascular density (MVD), vascular endothelial growth factor (VEGF), and hydroxyproline (HP) contents, and the extent of apoptosis in wound tissues at the end of the 12-day treatment period. Topical EPO treatment significantly reduced the time to final wound closure. This increased rate of closure of the two EPO-treated wounds in diabetic rats was associated with increased MVD, VEGF, and HP contents, and a reduced extent of apoptosis. In light of our finding that topical EPO treatment promotes skin wound repair in diabetic rats, we propose that topical EPO treatment is a therapeutically beneficial method of treating chronic diabetic wounds.Journal of Investigative Dermatology 08/2009; 130(1):287-94. · 6.31 Impact Factor -
Article: Topical negative pressure wound therapy: a review of its role and guidelines for its use in the management of acute wounds.
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ABSTRACT: Over the past two decades, topical negative pressure (TNP) wound therapy has gained wide acceptance as a genuine strategy in the treatment algorithm for a wide variety of acute and chronic wounds. Although extensive experimental and clinical evidence exists to support its use and despite the recent emergence of randomised control trials, its role and indications have yet to be fully determined. This article provides a qualitative overview of the published literature appertaining to the use of TNP therapy in the management of acute wounds by an international panel of experts using standard methods of appraisal. Particular focus is applied to the use of TNP for the open abdomen, sternal wounds, lower limb trauma, burns and tissue coverage with grafts and dermal substitutes. We provide evidence-based recommendations for indications and techniques in TNP wound therapy and, where studies are insufficient, consensus on best practice.International Wound Journal 10/2008; 5(4):511-29. · 1.46 Impact Factor -
Article: Assessment and management of persistent (chronic) and total wound pain.
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ABSTRACT: Persistent (chronic) wound-related pain is a common experience that requires appropriate assessment and treatment. It is no longer adequate for health care professionals to concentrate on the acute (temporary) pain during dressing change alone. The study provides useful recommendations and statements for assessing and managing total wound-related pain for patients, health care professionals and other policymakers. The recommendations have been developed with the involvement of an interprofessional panel of health care professionals from around the world.International Wound Journal 07/2008; 5(2):205-15. · 1.46 Impact Factor -
Article: A critical analysis of measurements used to assess and manage scars.
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ABSTRACT: Scars evolve through a maturation stage during which it is necessary to adapt different treatments. To adapt treatments, it is necessary to assess various parameters linked to inflammation. To this end, clinical scar assessments are subjective though reliable, and validation is operator dependent. The Vancouver Scar Scale, Visual Analogic Scale, Patient and Observer Scar Assessment Scale, and the Manchester Scale assess different scar characteristics. These scales are interesting, depending on the type of scars, and are easy to use but subject to errors. To use clinical a scale, the raters must be trained. Parameters can also be precisely assessed by technical means, whereby they rate only one parameter, but are more accurate. Some scales are easy to use, have low cost, and can be used for clinical assessment. Others scales are more complex and expensive, and can be used in research or treatment evaluation.The International Journal of Lower Extremity Wounds 01/2008; 6(4):249-53. · 1.20 Impact Factor -
Article: Evidence-based medicine: vacuum-assisted closure in wound care management.
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ABSTRACT: A review of the evidence base for the use of vacuum-assisted closure (VAC) therapy is presented, analysing both experimental and clinical data.International Wound Journal 10/2007; 4(3):256-69. · 1.46 Impact Factor -
Article: TIME heals all wounds.
Nursing Management 05/2004; 34(4):36-41; quiz, 41-2. -
Article: Wound bed preparation: a systematic approach to wound management.
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ABSTRACT: The healing process in acute wounds has been extensively studied and the knowledge derived from these studies has often been extrapolated to the care of chronic wounds, on the assumption that nonhealing chronic wounds were simply aberrations of the normal tissue repair process. However, this approach is less than satisfactory, as the chronic wound healing process differs in many important respects from that seen in acute wounds. In chronic wounds, the orderly sequence of events seen in acute wounds becomes disrupted or "stuck" at one or more of the different stages of wound healing. For the normal repair process to resume, the barrier to healing must be identified and removed through application of the correct techniques. It is important, therefore, to understand the molecular events that are involved in the wound healing process in order to select the most appropriate intervention. Wound bed preparation is the management of a wound in order to accelerate endogenous healing or to facilitate the effectiveness of other therapeutic measures. Experts in wound management consider that wound bed preparation is an important concept with significant potential as an educational tool in wound management. This article was developed after a meeting of wound healing experts in June 2002 and is intended to provide an overview of the current status, role, and key elements of wound bed preparation. Readers will be able to examine the following issues; the current status of wound bed preparation; an analysis of the acute and chronic wound environments; how wound healing can take place in these environments; the role of wound bed preparation in the clinic; the clinical and cellular components of the wound bed preparation concept; a detailed analysis of the components of wound bed preparation.Wound Repair and Regeneration 04/2003; 11 Suppl 1:S1-28. · 2.91 Impact Factor
Top co-authors
Top Journals
Institutions
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2009–2011
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Technion - Israel Institute of Technology
- Ruth and Bruce Rappaport Faculty of Medicine
Haifa, Haifa District, Israel
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2008
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University of Toronto
Toronto, Ontario, Canada -
Université de Montpellier 2
Montpellier, Languedoc-Roussillon, France
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