Roberto Cremonini

Publications (5)12.29 Total impact

• Article: The effects of nano-TiO2 on seed germination, development and mitosis of root tip cells of Vicia narbonensis L. and Zea mays L

  Monica Ruffini Castiglione, Lucia Giorgetti, Chiara Geri, Roberto Cremonini
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  ABSTRACT: This study aimed to provide new information about phyto-toxicology of nano-TiO2 on plant systems. To contribute to the evaluation of the potential harmful effects of the nanoparticles on monocots and dicots we considered their effects on seed germination and root elongation applying a concentration range from 0.2 to 4.0‰ in the plants Zea mays L. and Vicia narbonensis L. Moreover, we achieved a genotoxicity study at cytological level in root meristems by means of traditional cytogenetic approach, to evidence possible alterations in mitotic activity, chromosomal aberrations, and micronuclei release. From these analyses it comes out that nano-TiO2 particles, after short-term exposure and under our experimental conditions, delayed germination progression for the first 24h in both materials. Root elongation was affected only after treatment with the higher nano-TiO2 concentration. Further significant effects were detected showing mitotic index reduction and concentration-dependent increase in the aberration emergence that evidenced a nano-TiO2-induced genotoxic effect for both species. KeywordsChromosome aberrations–Genotoxicity–Mitotic activity–Nanoparticles–Nanotechnology EHS
  Journal of Nanoparticle Research 04/2012; 13(6):2443-2449. · 3.29 Impact Factor
• Source

  Available from: Gianfranco Venora

  Article: DNA methylation pattern in a barley reconstructed karyotype with deleted ribosomal gene cluster of chromosome 6H.

  Monica Ruffini Castiglione, Gianfranco Venora, Concetta Ravalli, Kostadin Gecheff, Lubomir Stoilov, Roberto Cremonini
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  ABSTRACT: A reconstructed barley karyotype (T-35) was utilised to study the influence of chromosomal rearrangements on the DNA methylation pattern at chromosome level. Data obtained were also compared with the distribution of Giemsa N-bands and high gene density regions along the individual chromosomes that have been previously described. In comparison to the control karyotype (T-1586), the DNA methylation pattern was found to vary not only in the reconstructed chromosomes but also in the other chromosomes of the complement. Significant remodelling process of methylation pattern was found also in the residual nucleolus organiser regions (NOR) on chromosome 5H as a consequence of deletion comprising the whole NOR of chromosome 6H in T-35. Moreover, differences between corresponding segments of the homologues with respect to some other chromosome locations were also observed. Repositioning of genomic DNA methylation along the metaphase chromosomes following chromosomal reconstruction in barley seems to be essential to ensure correct chromatin organisation and function.
  Protoplasma 02/2010; 242(1-4):13-8. · 1.92 Impact Factor
• Article: Methylated-rich regions and tandem repeat arrays along the chromosome complement of Colpodium versicolor (Stev.) Schmalh.

  Monica Ruffini Castiglione, Violet Kotseruba, Roberto Cremonini
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  ABSTRACT: The grass Colpodium versicolor (Stev.) Schmalh is one of six angiosperms with extremely reduced chromosome set 2n = 2x = 4. The chromosome complement of this species was studied. The DNA methylation pattern was determined with a specific monoclonal antiboby. 5-Methylcytosine residues are present in different chromosomal sites, with specific occurrence, some methylated bands showing differences between homologous chromosomes. Moreover, a fluorescent in situ hybridisation with telomere repeats and 45S rDNA sequences were performed. Hybridisation signals of telomeric repeats are detectable at the distal ends of the two pair of chromosomes, while 45S rDNA is localised in one chromosomal site, corresponding to the secondary constriction. In addition, 45S rDNA, as well as telomere-associated sequences, results to be 5-methylcytosine-enriched. The results are discussed and compared with those previously obtained in other plant systems 2n = 4 with the aim to enable a better knowledge of the lengthwise differentiation of this chromosome complement.
  Protoplasma 08/2009; 237(1-4):13-8. · 1.92 Impact Factor
• Article: 1,5-Anhydroxylitol from leaves of Olea europaea.

  Elisabetta Campeol, Guido Flamini, Pier Luigi Cioni, Ivano Morelli, Felicia D'Andrea, Roberto Cremonini
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  ABSTRACT: 1,5-Anhydroxylitol, a compound never found previously in the vegetal kingdom was obtained from Olea europaea leaves in approximately 0.5-1% yield.
  Carbohydrate Research 12/2004; 339(16):2731-2. · 2.33 Impact Factor
• Article: Volatile fractions from three cultivars of Olea europaea L. collected in two different seasons.

  Elisabetta Campeol, Guido Flamini, Pier Luigi Cioni, Ivano Morelli, Roberto Cremonini, Lucia Ceccarini
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  ABSTRACT: The chemical composition of the volatile fractions from leaves of three Olea europaea L. cultivars (Leccino, Frantoio, and Cipressino) harvested at two different times of the year were examined by GC and GC-MS. The results showed a high content of aliphatic aldehydes in the three cultivars during both harvesting periods and an increase of (E)-2-hexenal (an aldehyde with high antimicrobial properties) percentage from July to November.
  Journal of Agricultural and Food Chemistry 04/2003; 51(7):1994-9. · 2.82 Impact Factor