ABSTRACT: To study the value and feasibility of a novel method of urethrovesical anastomosis during radical laparoscopic prostatectomy.
From 2008 to 2009, 10 patients with local prostate cancer (LPC) underwent radical laparoscopic prostatectomy through the placement of two separate needles for running urethrovesical anastomosis. The first step comprised the first stitch placed in the posterior wall of anastomosis (at 4 o'clock) and then clockwise running suture from 4 to 12 o'clock position. The next steps entailed another needle, stitching at 3 o'clock position and counterclockwise running suture. Two sutures would meet at 12 o'clock position for the third and final knot. When the position of urinary leak was observed, an additional suture would be performed.
This technique was performed in 10 patients with a mean anastomosis duration of 30 minutes (range: 25 - 45) and a mean operative duration of 220 minutes (range: 200 - 300). The Foley catheter was implanted for 14 days. Neither bladder neck stricture nor urinary leak was observed with a follow-up period of 3 - 24 months.
The described technique is a feasible and safe method for urethrovesical anastomosis with a low rate of complications. And it may be quickly mastered so as to lower the learning curve of a novice.
Zhonghua yi xue za zhi 07/2010; 90(28):1955-7.
ABSTRACT: To investigate the growth of prostate cancer in vitro or in vivo by inhibiting the expression of EGFR and its intracellular effective proteins with small RNA interference ( SiRNA).
The hormone independence prostate cancer (HIPC) cell line PC-3 was transfected by EGFR SiRNA synthesized and cloned into a recombinant lentivirus vector. The growth rate of transfected PC-3 cell was measured by MTT. The expression of EGFR and the expression and phosphorylation of its intracellular proteins, such as Akt and MAPK, were detected by fluorescent Real-Time PCR and Western blot respectively. Meanwhile nude mice were transplanted with PC-3 cell to establish the tumor model and the tumor growth was observed.
The transfection efficiency was stable over 75% in PC-3 cell transfected with the recombinant lentivirus vector carrying EGFR SiRNA and the survival rate of PC-3 cell was only 40%-50%. Such depressant effects might be obtained by inhibiting the expression of EGFR mRNA and protein to only 10% as compared with their untreated levels (P < 0.01); meanwhile, the expression level and phosphorylation of Akt and MAPK also obviously decreased to 76.49% and 47.15% respectively (P < 0.05). Compared with the control group, the proliferation activity of tumors in nude mice was inhibited significantly by 34.83% (P < 0.05).
Lentivirus-mediated EGFR SiRNA can inhibit the growth of HIPC in vivo and in vitro by effectively suppressing the expression of EGFR and its intracellular proteins. The latter may be a potential candidate for future targeted therapy.
Zhonghua yi xue za zhi 08/2009; 89(32):2296-300.
ABSTRACT: To evaluate the feasibility and clinical efficacy of employing a homemade lasso to control the renal blood flow in retroperitoneal laparoscopic nephron-sparing surgery for the treatment of renal tumors.
Eight patients with benign renal tumors and 14 with malignant renal tumors underwent enucleation and wedge resection of renal tumors through retroperitoneal laparoscopy. Of these 22 patients, 15 were males and 7 females with a mean age of 45 years old. The mean tumor diameter was 3.2 cm.
All the procedures were technically successful without conversion to open surgery in all 22 patients. The mean operative time was 150 min and mean blood loss 80 ml. The mean postoperative hospital stay was 8 days. No intraoperative or postoperative complication occurred. All 14 cases of malignant renal tumors had a negative surgical margin. With a follow-up of 5-29 months, no recurrence occurred.
Retroperitoneal laparoscopic partial nephrectomy is feasible and safe for selective patients with renal tumors. Using a homemade lasso to control the renal blood flow in retroperitoneal laparoscopic partial nephrectomy is simple and less interferential.
Zhonghua yi xue za zhi 07/2009; 89(28):1983-5.
ABSTRACT: OBJECTIVE To observe and prove clinical therapeutic effect of Chinese drugs acupoint injection therapy on functional and mild arterial low-level blood supply erectile dysfunction (ED).
According to randomized and controlled method, 150 cases of functional and arterial low-level blood supply erectile dysfunction were divided into 3 groups, a Chinese drugs acupoint injection group, a saline acupoint injection and a Huichun Ruyi capsules group, 50 patients in each group. They were treated by acupoint injection of Danshen Injection and Chaihu Injection, acupoint injection of saline into Guanyuan (CV 4), Zuwuli (LR 10) and Huiyin (CV 1), and oral administration of Huichun Ruyi capsules, respectively. The changes of II EF-5 score, symptoms and signs, serum sexual hormones, Coprus Spongiosum PSV before and after the treatment were observed and compared.
The total effective rate was 92.0% in the Chinese drugs acupoint injection group, 84.0% in the saline acupoint injection group, and 66.0% in the Huichun Ruyi capsules group, with significant or very significant differences among the 3 groups (P < 0.05 or P < 0.01), and a very significant difference was shown in changes of II EF-5 score and Corus Spongiosum PSV before and after the treatment in the Chinese drugs acupoint injection group (P < 0.01), which were better than those in the saline acupoint injection group and the Huichun Ruyi capsules group (P < 0.05). No significant changes were found in levels of testosterone (T), follicule-stimulating hormone (FSH), luteotropic hormone (LH) prolactin (PRL) before and after the treatment in the 3 groups (P > 0.05).
Acupoint injection of Danshen Injection and Chaihu Injection has a definite curative effect on functional and arterial low-level blood supply ED. This therapy can raise II EF-5 score obviously and improve Corus Spongiosum PSV. The total effect of Chinese drugs acupoint injection group is better than that of saline acupoint injection or Huichun Ruyi capsules.
Zhongguo zhen jiu = Chinese acupuncture & moxibustion 12/2007; 27(12):881-5.
ABSTRACT: To compare and evaluate the clinical value among transperitoneal, retroperitoneal laparoscopic adrenalectomy and open adrenalectomy for the treatment of adrenal tumours.
From January 1996 to June 2002, transperitoneal laparoscopic adrenalectomy (TLA) was performed for 19 cases, 17 cases were successful (Group A), retroperitoneal laparoscopic adrenalectomy (RLA) for 21 cases, 19 cases were successful (Group B), during the same period 41 patients with adrenal tumours under 6 cm in diameter underwent open adrenalectomy (Group C). The procedure was evaluated and compared in the respects of tumors weight, operation time, blood loss, recovery time of GI function, hospital stay, complications transferable rate and indications among three groups.
Average tumors weight was (13.86) g in group A, (15.66) g in group B and (18.03) g in group C; mean operating time: group A was 17.21 min longer than group B, group A. B was 48.53 min longer and 31.32 min longer han group C respectively, mean blood loss in group A. B was less diminished by 121.55 ml and 137.05 ml than group C, and the blood loss in group B was less diminished by 15.50 ml than group A; mean recovery of Gl function, group A and B was 1.87 day and 2.17 day earlier than group C respectively, group B was 0.3 day earlier than group A, mean hospital stay was 2.60 day and 3.87 day shorter than group C, group B was 1.27 day shorter, than group A; transferable rate of operation was 10.5% (group A) and 9.5% (group B). Postoperative complication rate was 10.5%, 14.3% and 9.7% in group A. B and C respectively.
TLA and RLA were better than open method. On the hand of approach to adrenal gland disturbs to abdominal organ and postoperative recovery on RLA have some slight advantage.
Zhonghua wai ke za zhi [Chinese journal of surgery] 09/2003; 41(8):617-9.