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Publications (5)4.73 Total impact

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    ABSTRACT: Aim The aim of the study was to assess the importance and usefulness in everyday clinical practice of the urinary concentrations of β-microglobulin (β2M) and retinol binding protein (RBP) in evaluation of the renal function in HIV-positive patients. Background Kidney proximal tubule cells are the most prone to the damage and for low molecular-weight proteins as β-microglobulin (β2M) and retinol binding protein (RBP) are sensible markers of the pathology of this part of the nephron. Materials and methods Urine collected from 86 HIV-positive patients consulted in HIV/AIDS Outpatients Clinic in Wroclaw. Results In the group with decreased base-line GFR on tenofovir β2M level was statistically significant higher than in group on tenofovir with normal base-line GFR (p ≤ 0.05), in group on other antiretroviral treatment (p ≤ 0.001) and in cART-naïve patients (p ≤ 0.001). Mean RBP levels in groups on tenofovir both with decreased and normal baseline GFR were statistically significant higher (p ≤ 0.01; p ≤ 0.01) than in controls. There is statistically significant (p ≤ 0.05) negative linear correlations between the urinary concentrations of β2M and RBP and GFR as well as between urinary β2M and serum creatinine level (p ≤ 0.05) in a group of patients receiving cART with tenofovir. Conclusion β2M and RBP concentrations in urine may become useful in diagnosis of renal failure and are more sensitive than the standard determination of creatinine in plasma and eGFR in patients treated with tenofovir. The determination of the excretion of β2M seems a better measurement of tenofovir nephrotoxicity than RBP.
    HIV & AIDS Review. 01/2014;
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    ABSTRACT: Introduction: An increasingly important issue in the Polish population is drug abuse. It leads to extensive damage of parenchymal organs, including kidney. Establishing early markers of organ damage and their monitoring during rehabilitation therapy is therefore of pivotal importance. This study evaluated the utility of highly specific and selective markers (NGAL, IL-18, a and π-GST isoenzyme, and ß2-M). The influence of opioid drugs and other factors on kidney function (HIV and HCV infections, duration and the kind of drugs abused) was determined. Materials and Methods: Urine collected from 83 subjects who abused drugs and 33 healthy volunteers was tested with ELISA using specific antibodies (IBL, Biotron, Bioporto-Diagnostics). HIV infection was confirmed with western-blotting and HCV with PCR. CD4 lymphocytes were quantified with flow cytometry. RFLP and PCR were used to determine the viral load of HIV and HCV (genotype). Results: A significant increase of IL-18, NGAL and β2M activity in heroin addicts compared to the control group was noted as well as the influence of HIV infection on NGAL and β2M excretion. A statistically significant (p=0.04) correlation between the viral load and IL-18 concentration was noted while no significant influence of the duration and the kind of drugs abused, the route of intake or the age of addicts was seen. Only the NGAL concentration was sex dependent and significantly higher in women. Discussion: This study showed the specific, clinical utility of IL-18, NGAL, and β2M in the evaluation of renal function in drug addicts. Early detection of nephropathy with biochemical indicators might help prevent severe conditions that require hospitalization and intensive care.
    Postępy Higieny i Medycyny Doświadczalnej (Advances in Hygiene and Experimental Medicine) 01/2013; 67:1214-21.
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    ABSTRACT: Calcineurin inhibitors improve kidney allograft survival in the posttransplantation period; however, they may cause nephrotoxicity. The objective of this study was to compare the effect of cyclosporine (CsA) and tacrolimus (Tac) treatment on the transplanted kidney. The study included 219 patients aged 21 to 65 years. Of these, 120 (39 women and 81 men) were treated with CsA and 99 (38 women and 61 men) were treated with Tac. Patients were divided into 4 groups depending on the time since kidney transplantation. We evaluated urine markers of nephrotoxicity: proximal tubular cells lysosomal enzymes (N-acetyl-beta-d-glucosaminidase [NAG] and its isoform NAG-B, beta-d-galactosidase, and beta-glucouronidase) and brush border enzymes (alanyl aminopeptidase and gamma-glutamyl transpeptidase). Urine activities of nephrotoxicity markers were compared in CsA- and Tac-treated patients groups depending on the duration of treatment and allograft function as measured by serum creatinine concentration. Correlation studies between CsA and Tac levels and enzyme activities were performed in both groups and in the entire patient cohort. NAG and its isoform NAG-B seemed to be the most reliable markers of nephrotoxicity. Despite the significant correlation between NAG urine activity and serum creatinine concentration in the CsA group, there were no significant differences in NAG or NAG-B activities between CsA- and Tac-treated graft recipients.
    Transplantation Proceedings 07/2009; 41(5):1660-5. · 0.95 Impact Factor
  • Transplantation 01/2008; 86. · 3.78 Impact Factor
  • Toxicology Letters - TOXICOL LETT. 01/2006; 164.