Yong Jiang

Tianjin University of Science and Technology, T’ien-ching-shih, Tianjin Shi, China

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Publications (13)8.64 Total impact

  • Yong Jiang, Zhe Sun, Tong-cun Zhang
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    ABSTRACT: The purpose of the experiment: to explore the primary cultural method of vascular endothelial cell in rat that we have got through obtained and cultivated, the experiment were provided to the corresp technique of the further research. Experimental methods: the aorta of Wistar rat was obtained in operating microscope, then removal the adventitia and adipose tissue. The blood vessel were sheared like the organization by 1mm×1mm. Using stationary culture in DMEM/F12 culture medium contained fetal bovine serum (FBS). The results of experiment: endothelial cell swimmed from the borderline of organization to grow by adherence after three days. Then the cell began to synthesis after 10 to 12 days and were confluenced to monolayer about 70%. The passage cell were grew prosperity from the digest of diastase vera and were converged like the cobblestone characteristic of endothelial cell. The method of experiment was convenient and economy, we can obtain vascular endothelial cell in high fineness without collagenase and the supplement of endothelial cell in cell growth.
    01/2011;
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    ABSTRACT: Recent studies have demonstrated that the expression of aquaporin1 (AQP1) in endothelia cells played important roles in cell migration, and migration-associated cell function such as wound healing, and neutrophil motility. In the present study, we provided evidence that AQP1 expression in vascular endothelia cells (VECs) during angiogenesis was associated with MRTF-A. Morphology and immunofluorescence assay demonstrated that VECs from normal and angiogenesis tissues had similar appearance. Reverse Transcription-Polymerase Chain Reaction (RT-PCR), Immunoblot and Immunofluorescence analysis identified that AQP1 expression was significantly higher in angiogenesis VECs than in normal VECs. MRTF-A expression was also remarkably enhanced in angiogenesis VECs as compared to normal VECs, assessed by RT-PCR. The bioinformatics analysis found that the CarG element existed in the promoter region of AQP1 gene of many familiar mammals, including of mouse, rat, human etc. These results were the first to indicate that AQP1 was a target gene which could be regulated by MRTF-A/SRF.
    01/2010;
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    Yong Jiang, Zhi-Bin Jiang
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    ABSTRACT: Recent studies have demonstrated that aquaporin (AQP) expression facilitates cell migration and pro-motes angiogenesis and neutrophil motility. Migra-tion of tumor cells is a crucial step in tumor invasion and metastasis. Here we investigated the expression of AQP in MCF-7 human mammary carcinoma cells and characterized its function in cell migration. Re-verse Transcription–Polymerase Chain Reaction, Immunoblot and Immunofluorescence analysis dem-onstrated two populations of MCF-7 cell clones with low (AQP1L) and high (AQP1H) AQP1 expression and the AQP1 protein expression patterns in the plasma membrane of MCF-7 cells. MCF-7 cell clones (AQP1L and AQP1H) with low and about two-fold higher osmotic water permeability were identified by functional assays with corresponding low and high AQP1 expression. Cell migration rate was remarka-bly higher in AQP1H cells as compared to AQP1L cells, assessed by wound healing and transwell mi-gration assays. Adenoviral-mediated mRNA and pro-tein expression of AQP1 in AQP1L cells increased their water permeability and migration rate to the level similar to AQP1H cells. The results provided direct evidence that aquaporin-mediated plasma membrane water permeability played an important role in mammary carcinoma cell migration and may be associated with mammary carcinoma invasion and metastasis.
    Journal of biomedical science and engineering 01/2010; 3:95-100. · 0.27 Impact Factor
  • Yong Jiang
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    ABSTRACT: Recent studies revealed an important role of aquaporins (AQPs) in cell migration and migration-associated cell function such as angiogenesis, wound healing, and neutrophil motility. Migration of tumor cells is a crucial step in tumor invasion and metastasis. In the present study, we investigated the expression of AQP1 in human HT20 colon cancer cells and characterized its function in cell migration. By reverse transcription-polymerase chain reaction (RT-PCR) and immunoblot analysis, expression of AQP1 was identified in HT20 cell lines. Immunofluorescence analysis indicated expression of AQP1 protein in the plasma membrane of HT20 cells. The recombinant adenovirus expressing human AQP1 increased the mRNA and protein expression of AQP1 in HT20 cells. In contrary, the RNA interference vector of AQP1 effectively inhibited the mRNA and protein expression of AQP1 in HT20 cells. Adenovirus-mediated high expression of AQP1 in HT20 cells increased relative plasma membrane water permeability and migration rate in both wound healing and invasive transwell migration assays. In contrary, RNA interference vector-mediated low expression of AQP1 in HT20 cells reduced relative plasma membrane water permeability and migration rate. AQP1 expression induced relocalization of actin protein and activation of RhoA and Rac. In nude mice, AQP1 increased extravasation of HT20 Cells in lung after tail vein injection. The results provided the direct evidence that aquaporin-mediated plasma membrane water permeability plays an important role in colon cancer cell migration and may be associated with colon cancer invasion and metastasis.
    International Union of Biochemistry and Molecular Biology Life 10/2009; 61(10):1001-9. · 2.79 Impact Factor
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    ABSTRACT: Promoter fragment of alkaline protease gene was cloned from Bacillus alcalophillus PB92 genome by TAIL-PCR. The fragment was sequenced and analyzed, then submitted to GenBank (EU130686). The results showed that it contained several typical promoter charactered regions and two reverse translation frames located in - 538 to - 370 bp and - 275 to - 128 bp region. Deletion analysis of the sequence indicated that 414 bp upstream of the TSS exhibited predominant promoter activity, while an 105 bp length could serve as this function. Additionally, our data demonstrated that a representative Sec- type signal peptide structure presented in PB92 alkaline protease signal peptide. The efficiency of P<sub>aprE</sub>- AprE signal peptide gene cassette was validated by its driving a plant sweet protein monellin gene highly expression in Bacillus subtilis 1A751.
    Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on; 07/2009
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    ABSTRACT: Bone marrow mesenchymal stem cells (MSCs) can differentiate into smooth muscle cells (SMCs) and have tremendous potential for cell therapy and tissue engineering. In this study, to understand the effects of TGF-beta3 on rat bone marrow-derived MSCs and the underlying molecular mechanism of this differentiation process, we investigated that the changes of myocardin-related transcription factors (MRTFs) at the transcriptional level after rat MSCs were treated with TGF-beta3. The results showed that TGF-beta3 increased the expression of contractile genes, such as SM22, smooth muscle-myosin heavy chain (SM-MHC), SM-alpha-actin in MSCs. When TGF-beta3 induced MSCs differentiation into SMCs, myocardin and MRTF-A were activated. The data indicated that TGF-betai3 induced rat bone marrow-derived MSCs differentiation into SMCs by activating mypcardin and MRTF-A.
    Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on; 07/2009
  • Yong Jiang
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    ABSTRACT: The asparagine-proline-alanine sequences (NPA motifs) in Loops B and E of aquaporin are highly conserved. To investigate the role of two NPA motifs in the structure and function of aquaporin water channels, we generated human aquaporins (AQP)-1 mutations with NPA1 deletion, NPA2 deletion and NPA1,2 double deletion. Immunoblotting and immunofluorescence analysis indicated that all the three human AQP1 mutants possessed identical protein pattern and similar plasma membrane expression pattern compared to wild-type AQP1. Plasma membrane osmotic water permeability analysis, measured by YFP-based fluorescence quenching method and Xenopus oocyte expression assays, demonstrated that NPA1 or NPA2 deletion significantly reduced human AQP1 water permeability nearly 50% compared to wild-type AQP1, while NPA1,2 double deletion had little effect on human AQP1 water permeability. These results provide evidence that NPA motifs are important for water permeation but not essential for the expression, intracellular processing and the basic structure of human aquaporin 1.
    International Union of Biochemistry and Molecular Biology Life 07/2009; 61(6):651-7. · 2.79 Impact Factor
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    ABSTRACT: The SET and MYND domain-containing protein 3 (SMYD3) gene was found to encode a novel histone methyltransferase involved in human cancer cells. It could specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including of several oncogenes (e.g., N-Myc, CrkL, Wnt10b, RIZ, and hTERT) and genes involved in the control of cell cycle (e.g., Cyclin G1 and CDK2) and signal transduction (e.g., STAT1, MAP3K11, and PIK3CB). To determine the effects of SMYD3 overexpression on cell transformation, serum dependence and apoptosis sensitivity, we expressed SMYD3 in NIH3T3 cells, and these cells showed several transformed phenotypes as demonstrated by foci formation and colony growth in soft agar. Besides, these transfectants also showed increased serum dependence and depression of sensitivity to apoptosis induced by dexamethasone. These findings lend further understanding to the role of SMYD3 in the genesis of human cancers and might throw light on the development of novel therapeutic approaches to human cancers.
    International Union of Biochemistry and Molecular Biology Life 07/2009; 61(6):679-84. · 2.79 Impact Factor
  • Yong Jiang, Xin Zhang, Rui Su, Nan Wang
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    ABSTRACT: Recent studies uncovered an important role of aquaporins in cell migration, and migration-associated cell function such as angiogenesis, wound healing, and neutrophil motility. Here we provide evidence that aquaporin expression is involved in MCF-7 human mammary carcinoma cell migration. Reverse transcription-polymerase chain reaction, immunoblot and immunofluorescence analysis demonstrated two populations of MCF-7 cell clones with low (MCF-7l) and high (MCF-7A) AQP1 expression and the AQP1 protein expression patterns in the plasma membrane of MCF-7 cells. MCF-7 cell clones (MCF-11 and MCF-7A) with low and about two-fold higher osmotic water permeability were identified by functional assays with corresponding low and high AQP1 expression. Cell migration rate was remarkably higher in MCF-7A cells as compared to MCF-7l cells, assessed by wound healing and Boyden chamber assays. Adenoviral-mediated AQP1 expression in MCF-7l cells increased their water permeability and migration rate to the level similar to MCF-7A cells. These results provide the direct evidence that aquaporin-mediated membrane water permeability enhances MCF-7 mammary carcinoma cell migration and may be associated with mammary carcinoma invasion and metastasis.
    01/2009;
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    ABSTRACT: Myocardin-related transcription factors A (MRTF-A) is a myocardin-related transcription factor that has been found strongly activated CarG box - containing genes through its direct binding to serum response factor (SRF). In the present study, the MRTF-A expression vector was constructed. The MTT assay showed that transfection of MRTF-A could significantly decrease the anti-tumor effect of tamoxifen on MCF-7 human breast cancer cells. The bioinformatics analysis found that the CarG element existed in the promoter region of COMT gene of many familiar vertebrates, including of human, rhesus macaque, chimpanzee, etc. The results of RT-PCR assay further showed that MRTF-A could enhance the transcription level of COMT. These results are the first to indicate that COMT might be a target gene which could be regulated by MRTF-A/SRF, and such transactivation event might be involved in the process of tamoxifen resistance.
    01/2009;
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    ABSTRACT: Recent studies have demonstrated that aquaporin (AQP) expression facilitates cell migration and promotes angiogenesis and neutrophil motility. Migration of tumor cells is a crucial step in tumor invasion and metastasis. In the present study, we investigated the expression of AQP in human W489 colon cancer cells and characterized its function in cell migration. Two populations of W489 cell clones with high (W489h) and low (W489l) AQP1 expression were identified by RT-PCR and immunoblot analysis. Immunofluorescence indicated expression of AQP1 protein in the plasma membrane of W489 cells. W489 cell clones (W489h and W489l) with high and about 2.3 fold lower osmotic water permeability with corresponding high and low AQP1 expression were identified by functional assay. Serum-induced transwell migration rate of W489l (9.5plusmn1.1%) was remarkably lower than that of W489h (28.9plusmn0.9%). Wound healing assay demonstrated significantly decelerated wound closure in W489l cells as compared to W489h cells. RNA interference vector of AQP1 (AQP1i) effectively inhibited the mRNA and protein expression of AQP1 in W489h cells. Accordingly, relative plasma membrane water permeability, transwell migration rate and wound closure speed of W489h- AQP1i were reduced to the level similar to W489l cells. The results provided direct evidence that aquaporin-mediated plasma membrane water permeability plays an important role in colon cancer cell migration and may be associated with colon cancer invasion and metastasis.
    01/2009;
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    ABSTRACT: Aquaporin (AQP) has been reported to express in microvessels of many different type of tumor, indicating the possible involvement of AQP in tumor angiogenesis. Tumor angiogenesis is a mandatory step of tumor growth involving endothelial cell proliferation and migration from blood vessels in peritumoral tissues and formation of tubules. A murine Hepal-6 hepatocellular carcinoma-bearing model in AQP1 knockout mice was used to evaluate the role of AQP1 in Hepal-6 hepatocellular carcinoma angiogenesis and tumor growth. The results demonstrated remarkably retarded growth of subcutaneously inoculated Hepal-6 cells in AQPl-knockout (AQP1-l-) mice compared to that in wild-type (AQP1+/+) mice. Morphology of Hepal-6 hepatocellular carcinoma sections revealed tumor cells formed islands with microvessels in the center. Immunohistochemistry of tumor blood vessels demonstrated high-density and thin microvessels located in the center of tumor islands in AQP1+/+ mice, whereas sparse and enlarged vessels were evident in tumor islands in AQP1-l- mice coincident with larger necrotic area in outer layer of the islands. Blood vessel AQP1 immune staining of Hepal-6 hepatocellular carcinoma sections indicated that strong AQP1 protein labelling in vascular endothelium cells of hepatocellular carcinoma in AQP1+/+ mice and negative labelling of AQP1 in the counterpart structures in AQP1-l- mice. These results provided direct evidence that AQP1 deletion results in defective Hepal-6 hepatocellular carcinoma angiogenesis and retarded tumor growth that may be involved in insufficient blood supply and abnormal transendothelial fluid transport.
    01/2009;
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    ABSTRACT: Bone marrow mesenchymal stem cells (MSCs) can dif-ferentiate into smooth muscle cells (SMCs) and have tremendous potential for cell therapy and tissue engi-neering. In this study, to understand the effects of TGF-β3 on rat bone marrow-derived MSCs and the underlying molecular mechanism of this differentiation process, we investigated that the changes of myocar-din-related transcription factors (MRTFs) at the tran-scriptional level after rat MSCs were treated with TGF-β3. The results showed that TGF-β3 increased the expression of contractile genes, such as SM22, smooth muscle-myosin heavy chain (SM-MHC), SM-α-actin in MSCs. When TGF-β3 induced MSCs differentiation into SMCs, myocardin and MRTF-A were activated. The data indicated that TGF-β3 induced rat bone marrow-derived MSCs differentiation into SMCs by activating mypcardin and MRTF-A.