ABSTRACT: Sirtuin1 (SIRT1) is a nicotinamide adenine dinucleotide-dependent deacetylase. Recently, some studies have suggested that SIRT1 could be over-expressed in breast, prostate and colon cancers and up-regulated SIRT1 inactivates p53 by deacetylation. Therefore, we investigated the prevalence and the prognostic impact of SIRT1 and p53 expression in ovarian epithelial tumours.
Immunohistochemical expression of SIRT1 and p53 were evaluated using tissue microarray in 40 cases of benign epithelial tumours, 36 cases of borderline tumours, and 90 cases of malignant tumours.
Expression of SIRT1 was significantly increased in malignant epithelial tumours compared to benign and borderline epithelial tumours (p < 0.001). In particular, a high proportion of serous carcinoma expressed SIRT1 (86%, 55/64 cases). Despite the frequent expression of SIRT1 in malignant ovarian epithelial tumours, serous carcinomas of high FIGO stage showed less frequent SIRT1 expression compared to that of low stage serous carcinomas (p = 0.029). Moreover, increased expression of SIRT1 in serous carcinoma correlated with increased overall survival by univariate (p = 0.014) and multivariate analyses.
Over-expression of SIRT1 may play an important role in the early stage of ovarian carcinogenesis.
Pathology 06/2009; 41(4):366-71. · 2.38 Impact Factor
ABSTRACT: Background and aims: Recently, activation of the local renin-angiotensin system via angiotensin II type 1 and 2 receptor (AT1R and AT2R) was shown under neoplastic conditions in certain tumor tissues. We aimed to evaluate the expression of AT1R and AT2R in ovarian epithelial tumors and its correlation with other clinicopathologic parameters including patient survival. Methods: Immunohistochemical staining for AT1R and AT2R were performed by using tissue microarray in 40 benign epithelial tumors (20 serous adenomas and 20 mucinous adenomas), 36 borderline tumors (11 serous tumors and 25 mucinous tumors), and 90 malignant tumors (64 serous carcinomas, 19 mucinous carcinomas, 5 endometrioid carcinomas, and 2 clear cell carcinomas). Additionally, immunostaining for Ki-67 was performed in 90 malignant epithelial tumors. Results: The expression of AT1R and AT2R were significantly correlated with malignant potential of tumors (P < 0.001 and P < 0.001, respectively) and histological grade of malignant tumors (P < 0.001 and P= 0.001, respectively). However, there was no prognostic significance of AT1R and AT2R in ovarian carcinomas. Conclusion: Although the exact roles of angiotensin II receptor in the development of ovarian epithelial tumor is not clear, this study suggests that both AT1R and AT2R may involved in development and progression of ovarian epithelial tumors.
Basic and Applied Pathology 11/2008; 1(3):131 - 138.
Pathology 09/2008; 40(5):534-6. · 2.38 Impact Factor