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ABSTRACT: Taking the Picea schrenkiana var. tianschanica forests at three sites with different longitudes (Zhaosu, Tianchi, and Qitai) in Tianshan Mountains as the objects, the cones were collected along an altitudinal gradient to analyze the variation of their seed morphological traits (seed scale length and width, seed scale length/width ratio, seed wing length and width, seed wing length/ width ratio, seed length and width, and seed length/width ratio). All the seed traits except seed width tended to decrease with increasing altitude. The seed traits except seed wing width, seed width, and seed length/width ratio all had significant negative correlations with altitude. Seed scale length and width and seed scale length/width ratio had significant positive correlations with longitude. Seed scale length, seed scale length/width ratio, and seed wing length/width ratio had significant negative correlations with slope degree. No significant correlations were observed between the seed traits except seed wing width and the slope aspect. Altitude was the main factor affecting the seed scale length, seed scale length/width ratio, and seed wing length/width ratio.
Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban 06/2012; 23(6):1455-61.
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Ling Li,
Bin Wang,
Peng Li, Zhi-qiang Bai,
Hai-tao Wang,
Hai-yan Liu,
Dong-meng Qian,
Xiu-xia Song,
Shou-yi Ding,
Zhi-yong Yan,
Guang-yu Jiang
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ABSTRACT: HCMV is a major cause of congenital brain disease in humans, and its neuropathogenesis is not yet fully understood. The objective
of the present study is to investigate the effect of human cytomegalovirus (HCMV) infection on human hippocampus neural precursor
cell (NPCs) differentiation in vitro. Fetal hippocampus tissue was dissociated mechanically and then cultured in proliferation medium with EGF and bFGF. The identification
and purity of the NPCs were confirmed by using immunofluorescence to detect the expression of the NPCs marker-Nestin. To drive
NPCs differentiation, bFGF and EGF were withdrawn from the medium and replaced with FBS (10%). HCMV AD169 (MOI=5) was added
into the differentiation medium at the onset of the differentiation. After 7 days of differentiation, in order to confirm
whether NPCs are permissive for HCMV infection, immunofluorescence was used to stain for the presence of immediate early (IE)
and late (pp65) HCMV proteins in the infected cells. The effects of HCMV infection on NPCs’ differentiation was observed by
detecting the ratio of nestin and GFAP positive cells with confocal microscopy and immunofluorescence. The data showed that
95%±8% of the cells (passage 4–8) cultured were Nestin positive which suggested that majority of the cells were NPCs. On day
7 postinfection, most of the infected cells were IE and PP65 positive. The percentage of Nestin-positive cells were 93%±10%
and 50%±19% (t=6.03, p<0.01) and those of GFAP-positive cells were 55±17% and 81%±11% (t=3.77, p<0.01) in HCMV treated and control groups respectively. These findings indicate that NPCs are HCMV permissive cells and HCMV
(AD 169) infection suppresses the differentiation of Hippocampus-genetic human NPCs into astrocytes. These effects may provide
part of the explanation for the abnormalities in brain development associated with congenital HCMV infection.
Virologica Sinica 04/2012; 24(6):552-558.
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ABSTRACT: ObjectiveTo provide a new approach for the treatment of tumor in Meckel’s cave, by dissecting adjacent structures of the nasal cavity-maxillary
sinus-pterygopalatine fossa-Meckel’s cave approach.
MethodsFifteen adult cadaver heads (30 sides) were dissected and the correlated anatomic landmarks were observed, measured and analyzed
in an operative route.
ResultsThe approach was divided into 3 steps: entering the maxillary sinus, the later pterygopalatine fossa and the final Meckel’s
cave. Safe access to Meckel’s cave could be achieved by tracing the vidian neurovascular bundles and dissecting the quadrangular
space (QS). The distances from the nasal columella to the apertura maxillaries, the sphenopalatine foramen, and the anterior
foramen of the pterygoid canal were (44.08±2.61) mm, (64.83±2.42) mm, and (70.43±2.94) mm, respectively. The angles between
the horizontal plate of the palatine bone and the link from nasal columella to apertura maxillaries, between the horizontal
plate of the palatine bone and the link from nasal columella to sphenopalatine foramen were (38.10±2.46)° and (26.15±2.26)°,
respectively.
ConclusionThe endoscopic approach of transnasal maxillary sinus-pterygopalatine fossa-Meckel’s cave (ENMPA) is a safe and direct way
to access Meckel’s cave, and could be employed for the treatment of tumor in Meckel’s cave.
目的通过对鼻腔-上颌窦-翼腭窝-Meckel 腔入路的内镜解剖学研究, 为治疗Meckel 腔肿瘤提供新的入路选择。
方法对15 具动静脉灌注乳胶的成人尸头标本进行模拟鼻内镜下该入路分层显微解剖, 对相关解剖标志进行了观察、 分析、 拍摄和测量。
结果该入路分为三步: 进入上颌窦, 进入翼腭窝, 最后进入Meckel 腔。 追踪翼맜神经血맜束, 解剖分离四方形空间可安全到达Meckel 腔。 鼻小柱至上颌窦口、 蝶腭孔、 翼맜前孔距离分别为(44.08±2.61) mm、 (64.83±2.42)
mm 和(70.43±2.94) mm。 鼻小柱至上颌窦口连线, 及其至蝶腭孔连线与腭骨水平板的夹角分别为(38.10±2.46)°和(6.15±2.26)°。
结论鼻内镜下鼻腔-上颌窦-翼腭窝-Meckel腔入路是到达Meckel腔前下内面的安全且直接的入路, 可用来治疗Meckel 腔肿瘤。
Neuroscience Bulletin 04/2012; 25(6):376-382. · 1.31 Impact Factor
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ABSTRACT: Human cytomegalovirus (HCMV) is the most common cause of congenital infection, resulting in birth defects such as microcephaly.
In this study, RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression
in neuroglia cells by HCMV infection. The results showed that basal, endogenous NGF expression in U251 was unchanged during
early HCMV infection. NGF expression is strongly down-regulated during the latent phase of infection. These results suggest
that HCMV can depress the NGF expression in U251 cells.
Virologica Sinica 04/2012; 24(3):209-214.
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ABSTRACT: Human herpes simplex virus 1 (HSV-1) causes facial, ocular, and encephalitic disease and is associated with latent infection
and cancer. Here, we developed a means of studying the pathogenesis of HSV-1 infection at the protein level by using the SELDI
Protein Chip to detect changes of protein expression in Vero cells cultured in vitro. After infection with HSV-1 and culture
for 12, 24 or 48 h, cells were harvested and lysed. IMAC3 arrays were applied to SELDI-TOF-MS to detect proteomic differences
before and after infection. The chip detected a series of differentially expressed protein peaks. Inte-restingly, both peaks
at 16 912 Da and 17 581 Da corresponded precisely with the molecular mass of ISG15, which may participate in antiviral activity
during the process of infection. Thus, the results we obtained can serve as a basis to study the pathogenesis of HSV-1 and
the interaction between the virus and its host. In addition, they can help in the discovery of new therapeutic targets for
treatment of HSV-1 infection.
Virologica Sinica 04/2012; 22(1):68-73.
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ABSTRACT: A vertical transect investigation on Picea schrenkiana var. tianschanica forests was conducted at five different longitudinal sites (Zhaosu, Gongliu, Wusu, Urumqi, and Hami) in Tianshan Mountains, and the distribution pattern of P. schrenkiana var. tianschanica population at each site was analyzed based on theoretical distribution model and aggregation intensity index. On the whole, the P. schrenkiana var. tianschanica population in Tianshan Mountains presented a clumped distribution, and the distribution pattern and clustering intensity were affected by the developmental stages of stem and the ranges of altitude to some degree. The clustering intensity increased with the increasing size (DBH) or developmental stage of stem, and had the highest values at high altitudes.
Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban 01/2011; 22(1):9-13.
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ABSTRACT: To provide a new approach for the treatment of tumor in Meckel's cave, by dissecting adjacent structures of the nasal cavity-maxillary sinus-pterygopalatine fossa-Meckel's cave approach.
Fifteen adult cadaver heads (30 sides) were dissected and the correlated anatomic landmarks were observed, measured and analyzed in an operative route.
The approach was divided into 3 steps: entering the maxillary sinus, the later pterygopalatine fossa and the final Meckel's cave. Safe access to Meckel's cave could be achieved by tracing the vidian neurovascular bundles and dissecting the quadrangular space (QS). The distances from the nasal columella to the apertura maxillaries, the sphenopalatine foramen, and the anterior foramen of the pterygoid canal were (44.08+/-2.61) mm, (64.83+/-2.42) mm, and (70.43+/-2.94) mm, respectively. The angles between the horizontal plate of the palatine bone and the link from nasal columella to apertura maxillaries, between the horizontal plate of the palatine bone and the link from nasal columella to sphenopalatine foramen were (38.10+/-2.46) degrees and (26.15+/-2.26) degrees , respectively.
The endoscopic approach of transnasal maxillary sinus-pterygopalatine fossa-Meckel's cave (ENMPA) is a safe and direct way to access Meckel's cave, and could be employed for the treatment of tumor in Meckel's cave.
Neuroscience Bulletin 12/2009; 25(6):376-82. · 1.31 Impact Factor
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ABSTRACT: To explore the change of endogenic nerve growth factor (NGF) expression in human glioma cells infected with human cytomegalovirus (HCMV).
U251 cells were cultured in RPMI 1640 culture medium and infected with HCMV AD169 strain in vitro to establish a cell model of viral infection. Morphologic changes of U251 cells were observed under inverted microscope before and after infection with HCMV. Expression of NGF gene and protein of cells was detected by RT-PCR and Western blotting before and after infection with HCMV.
The cytopathic effects of HCMV-infected cells appeared on day 5 after infection. However, differential NGF expression was evident on day 7. NGF expression was decreased significantly in U251 cells on day 7 after infection in comparison with control group (P < 0.05).
HCMV can down-regulate endogenous NGF levels in human glioma cell line U251.
Biomedical and Environmental Sciences 08/2009; 22(4):354-8. · 1.35 Impact Factor
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ABSTRACT: During the infection of host cells, IE2 protein is one of the first and most abundantly expressed products of HCMV genome, which plays an important role in the controlling of cell cycle and apoptosis. But the correlation between expression level and anti-apoptotic activity of IE2 protein is still not clear. In this study, we successfully established a HCMV IE2 protein expression cell line that was controlled by Tet-On system. The effect of IE2 protein on cell apoptosis and the expression of p53 was detected under different condition of induction. Our results showed that the IE2 protein could inhibit cell apoptosis induced by TNF-alpha. Additionally, the anti-apoptotic activity of IE2 protein seemed to be relevant to its expression level. However, we failed to detect any difference of p53 expression between the IE2 protein expression and non-expression cells. These data indicated that the IE2 protein might inhibit cell apoptosis through regulating different signal pathways.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] 06/2009; 25(3):190-5.
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ABSTRACT: The objective of present study is to investigate the effect of human cytomegalovirus (HCMV) infection on human hippocampus neural stem cells NSCs differentiation in vitro, Fetal hippocampus tissue was dissociated mechanically and then cultured in proliferation medium with EGF and bFGF. Immunofluorescence method was used to detect the expression of NSCs marker-Nestin within these cells. Cultured in 10% FBS, NSCs began to differentiate. On the onset of the differentiation, HCMV AD169 (MOI=5) was added into the differentiation medium. After 7 days differentiation, the effect of HCMV infection on NSCs differentiation was observed by detecting the rate of nestin, GFAP and HCMV immediate-early (IE) positive cells with confocal microscopy and immunofluorescence method. The resucts showed most of the cells (passage 4-6 ) were Nestin positive and could differentiate into NSE-positive neurons and GFAP-positive astrocytes. On day 7 postinfection, 86% +/- 12% of infected cells were IE positive. The percentage of Nestin-positive cells was 50% +/- 19% and 93% +/- 10% (t= 6.03, P<0.01)and those of GFAP-positive cells was 81% +/- 11% and 55 +/- 17% (t=3.77, P<0.01) in uninfected and infected cells respectively. These findings indicated that NSCs were HCMV permissive cell and HCMV AD 169 infection suppressed the differentiation of Hippocampus-genetic human neural stem cells into astrocytes.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] 05/2009; 25(3):196-201.
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ABSTRACT: MicroRNAs (miRNAs) play important roles in eukaryotes, plants and some viruses. It is increasingly clear that miRNAs-encoded by viruses can affect the viral life cycle and host physiology. Viral miRNAs could repress the innate and adaptive host immunity, modulate cellular signaling pathways, and regulate the expression of cellular and viral genes. These functions facilitate viral acute and persistent infections, and have profound effects on the host cell survival and disease progression. Here, we discuss the miRNAs encoded by herpesviruses, and their regulatory roles involved in virus-host interactions.
Virologica Sinica 12/2008; 23(6):459-472.
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ABSTRACT: The Picea schrenkiana var. tianschanica forest in Zhaosu, Gongliu, Wusu, Urumqi and Hami that covered about 12 longitudes (81 degrees 05'-93 degrees 41.5'E) along the Tianshan Mountains was investigated by using vertical transects to analyze the species richness of the forest along an altitudinal gradient at different longitudes. The results showed that at most longitudinal positions, P. schrenkiana var. tianschanica forest was basically of pure forest, with P. schrenkiana var. tianschanica as the dominant species, very few of other arbor and shrub species, and the species richness of herbs being controlled by the growth of P. schrenkiana var. tianschanica. This forest had the widest distribution, highest canopy density, best growth, and highest stand volume at middle altitudes, resulting in the lowest species richness of herbs. The herb species richness in P. schrenkiana var. tianschanica forest in Tianshan Mountains showed an inverse hump-shaped variation along the altitude, which could be described by the quadratic equation y = ax2 - bx + c (a, b, c > 0).
Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban 08/2008; 19(7):1407-13.
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ABSTRACT: By combining Fractional Spectral Subtraction (FSS) with Perceptual Linear Predictive (PLP), a hybrid method of noise robustness speech recognition isinvestigated in this paper. This method uses FSS for noisy speech to reduce noise components in the fractional Fourier domain. According to the results ofcomputing Itakura distance and Mean Square Error (MSE), an approximate optimal fractional order is then obtained by comparing the difference betweenthem. Perceptual Linear Predictive Cepstral Coefficients (PLPCC) is finally computed for the enhanced speech in terms of the above obtained order.It is shown that this hybrid method performs better compared with conventional spectral subtraction and PLPCC for digits speech recognition experiments. Moreover, this method denotes good noise robustness when noise levels increases.
Image and Signal Processing, 2008. CISP '08. Congress on; 06/2008
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ABSTRACT: ObjectiveTo detect differential protein expression in malignant and normal liver cell lines in vitro using the SELDI ProteinChip platform, for investigating the pathogenesis of liver cancer.
MethodsTwo cell lines, human normal liver cell line L02 and hepatoma cell line SMMC-7721 were cultured routinely, harvested in good
condition and lysed. After quantification, the supernatant of the lysate was tested by IMAC3 (Immobilized Mental Affinity
Capture) and WCX2 (Weak Cation Exchange) chips on the SELDI-TOF-MS ProteinChip reader.
ResultsProtein expression differed between the malignant and normal liver cell lines. A total of 20 differentially expressed proteins
were found, among which, 7 were captured by the IMAC3 chip and 14 by the WCX2 chip. Peaks at 5,419, 7,979 and 11,265 Da were
higher and at 8,103, 8,492, 10,160 and 11,304 Da lower in SMMC-7721 cells by the IMAC3 chip; peaks at 7,517, 7,945 and 7,979
Da were higher and at 5,061, 5,551, 5,818, 7,439, 9,401,10,100, 10,312, 11,621, 11,662, 11,830 and 12,772 Da lower in SMMC-7721
cells by the WCX2 chip. Interestingly, both chips captured the 7,979 Da peak. In addition, the 11,081 Da peak corresponded
precisely with the molecular mass of the calcium binding protein S100A10, which may participate in the formation of liver
cancer in association with p36.
ConclusionDetecting differential protein expression in malignant and normal liver cell lines using the SELDI ProteinChip platform was
simple, sensitive and repeatable. The results we obtained can serve as a basis for investigating the pathogenesis of liver
cancer and aid the discovery of new therapeutic targets.
Chinese Journal of Cancer Research 01/2007; 19(2):94-99. · 0.18 Impact Factor
