Wen Zhang

Publications (2)4.26 Total impact

• Article: Erratum to: Effect of Bilayer Phospholipid Composition and Curvature on Ligand Transfer by the alpha-Tocopherol Transfer Protein.

  Wen Zhang, Grant Frahm, Samantha Morley, Danny Manor, Jeffrey Atkinson
  Lipids 10/2009; · 2.13 Impact Factor
• Source

  Available from: Jeffrey Atkinson

  Article: Effect of bilayer phospholipid composition and curvature on ligand transfer by the alpha-tocopherol transfer protein.

  Wen Xiao Zhang, Grant Frahm, Samantha Morley, Danny Manor, Jeffrey Atkinson
  [show abstract] [hide abstract]
  ABSTRACT: We report here our preliminary investigations on the mechanism of alpha-TTP-mediated ligand transfer as assessed using fluorescence resonance energy transfer (FRET) assays. These assays monitor the movement of the model alpha-tocopherol fluorescent derivative ((R)-2,5,7,8-tetramethyl-chroman-2-[9-(7-nitro-benzo[1,2,5]oxadiazol-4-yl amino)-nonyl]-chroman-6-ol; NBD-Toc) from protein to acceptor vesicles containing the fluorescence quencher TRITC-PE. We have found that alpha-TTP utilizes a collisional mechanism of ligand transfer requiring direct protein-membrane contact, that rates of ligand transfer are greater to more highly curved lipid vesicles, and that such rates are insensitive to the presence of anionic phospholipids in the acceptor membrane. These results point to hydrophobic features of alpha-TTP dominating the binding energy between protein and membrane.
  Lipids 06/2009; 44(7):631-41. · 2.13 Impact Factor