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ABSTRACT: Numerous studies have demonstrated that endotoxin plays an important role in the development and progression of hepatic cirrhosis. However, there is no effective remedy for the prevention and treatment of intestinal endotoxemia. Taurine has been reported to have beneficial effects on endotoxemia. Oats have been shown to absorb intestinal toxins and increase excretion of intestinal toxins. The present study was to investigate whether a combination of taurine and oat has an additive inhibitory effect on endotoxin release in a rat liver ischemia/reperfusion model. Our results showed that the combination of taurine (300mgkg(-1)d(-1)) and oat fiber (15gkg(-1)d(-1)) significantly reduced endotoxin levels in the portal vein by 36.3% when compared to the control group (0.168+/-0.035Eu/ml in the treatment group vs 0.264+/-0.058Eu/ml in the control group, P<0.01). The treatment of taurine (300mgkg(-1)d(-1)) and oat fiber (15gkg(-1)d(-1)) induced 21.5% and 18.4% reduction in endotoxin levels, respectively, when compared to the control group (P<0.05). We conclude that the combination of taurine and oat fiber achieved an additive inhibitory effect on intestinal endotoxin release, which might be an effective approach for the treatment of intestinal endotoxemia.
Chemico-biological interactions 03/2010; 184(3):502-4. · 2.46 Impact Factor
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ABSTRACT: To explore the estrogenic effects and disruptive mechanism of NP and BPA by reporter gene-based assays we developed.
pERE-Luc plamid was generated by inserting estrogen response element (ERE) fragment into MCS of pGL3-promoter vector. MCF7 cells were cotransfected with pERE-Luc and phRL-SV40 using Sofast transfection reagent. The cells then treated with 17beta-estradiol (E2), tamoxifen (Tam), nonylphenol(NP) and bisphenol A (BPA) and expression of the repoter gene in the cell lysates was assayed using Dual-Lucferase reporter assay system.
The pERE-Luc plasmid was constructed. Luciferase activities of MCF7 cells transfected pERE-Luc showed dose-responed realitionship with E2. 1 x 10(-11) mol/L E2 could induce the expression of reporter gene and 1 x 10(-9) mol/L E2 resulted in the largest luciferase activity. E2 couldn't induce the luciferase activity without pERE-Luc. Tam is a complete antagonist, inhibited the E2-induced luciferase expression. NP induced the luciferase activity at concertrations > 1 x 10(-6) mol/L, BPA induced the luciferase activity at concertrations > 1 x 10(-6) mol/L. The estrogenic activity of NP was more than BPA.
The assay we established is usful, NP and BPA showed estrogenic activities.
Wei sheng yan jiu = Journal of hygiene research 01/2006; 35(1):13-5.
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ABSTRACT: To observe the clinical effect of cinobufacini injection in treating moderate and advanced primary liver cancer (PLC).
One hundred patients with moderate and advanced PLC were randomly divided into cino-treated group (50 patients) and control group (50 patients). The quality of life, tumor size, some changes of laboratory tests, and survival time were observed.
The progressive rate of cino-treated group (18%) was lower than that of the control group (32%). The quality of life of the cino-treated group (80%) was better than that of the control group (72%), but without statistical significance. The survival rate of >12 months of the cino-treated group (30%) was higher than that of the control group (18%). The patients' liver function such as serum total bilirubin and ALT decreased obviously in the cino-treated group while increased a lot in the control group. The level of AFP increased after treatment with statistical significance in the control group while there was no statistical significance in the cino-treated group.
Cinobufacini injection can not only inhibit the proliferation of cancer, but also protect liver function, improve quality of life and prolong survival time.
Journal of Chinese Integrative Medicine 10/2003; 1(3):184-6.
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ABSTRACT: Matrine (Mat), a component extracted from Sophora flavescens Ait, has a wide spectrum of pharmacological effects. Glycyrrhizin (Gly), a major active constituent of licorice (Glycyrrhiza glabra) root, has various pharmacological effects. Gly and Mat are ancillary drugs used clinically in China for protection of liver function and treatment of tumors. However, habitual administration of Gly may cause adverse effects marked by the development of pseudohypercorticosteroidism. This work was designed to see whether combination use of Gly and Mat could offer better liver protective and anti-hepatocarcinogenic effects than Gly or Mat alone, and whether it could reduce the adverse effects of Gly alone by acetaminophen-induced hepatotoxicity, diethylnitrosamine-induced hepatocarcinogenesis, induction of immunosuppression, albumen-induced swelling of rat hind paws. The results showed that compared with Gly or Mat alone, Gly + Mat reduced the mortality of acetaminophen overdosed mice more effectively, attenuate acetaminophen-induced hepatotoxicity, and reduced the number and area of γ-GT positive foci, thus protecting liver function and preventing HCC from occurring. In addition, Gly + Mat had a protective effect on immunosuppression, a strong non-specific anti-inflammatory effect, and an effect of reducing the incidence of sodium and water retention.
Chemico-Biological Interactions.
