Publications (23)38.08 Total impact
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Article: [The smallpox vaccines and the definitive destruction of the last virus strains].
Medicina Clínica 05/2011; 137(7):308-10. · 1.38 Impact Factor -
Article: [Role of the Sentinel Surveillance System in the Influenza A (H1N1) pandemic].
Enfermedades Infecciosas y Microbiología Clínica 02/2011; 29(3):240-2. · 1.49 Impact Factor -
Article: [Utility of a commercial antigenic detection method for diagnosing pandemic influenza a (H1N1)].
Enfermedades Infecciosas y Microbiología Clínica 02/2010; 28(8):558-9. · 1.49 Impact Factor -
Article: [General considerations about the new influenza A (H1N1)].
Medicina Clínica 10/2009; 133(16):626-8. · 1.38 Impact Factor -
Article: [Consecutive infections by different types and subtypes of influenza virus in the same epidemic season].
Enfermedades Infecciosas y Microbiología Clínica 09/2009; 28(4):257-8. · 1.49 Impact Factor -
Article: [Comparison study of a real-time reverse transcription polymerase chain reaction assay with an enzyme immunoassay and shell vial culture for influenza A and B virus detection in adult patients].
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ABSTRACT: The age of the patients and the type of sample are major problems in the diagnosis of influenza. Most available diagnostic techniques are highly effective in pediatric patients and in nasopharyngeal aspirates. However, in the adult population and using throat swabs, these techniques are much less reliable. We performed a prospective study comparing the efficacy of a commercial real-time reverse transcription PCR assay (RT-PCR) with that of an enzyme immunoassay (EIA) or shell vial culture (SV) in the detection of influenza A and B viruses in 125 throat swabs from adults with clinically suspected influenza during the 2007-2008 flu season. Throat swabs were subjected to rapid antigen detection for influenza viruses by means of a commercial dot-blot EIA. For the RT-PCR technique, RNA was extracted from 200 microL of each sample by the automated extraction system, EZ1 virus minikit (version 2.0). Genomic amplification of the extracted viral RNA was carried out using the OneStep RT-PCR FluA+FluB automated system with the SmartCycler amplification system. Each sample was inoculated into 2 SV of the MDCK cell line. Turnaround times were calculated from the time specimens were received in the laboratory to the time the result was reported to clinicians. The EIA system detected 27 (21.6%) positive samples, RT-PCR 62 (49.6%) positive samples, and SV 56 (44.8%) positive samples. Among the 62 positive samples, EIA detected 27 (43.5%), RT-PCR 62 (100%) and SV 56 (90.3%). With the use of RT-PCR, 38.4% of the adults studied were diagnosed on the same day samples were received. Among the total, 67.2% of diagnostic results were obtained within the first 24 hours; turnaround time was 1.1 days. The real-time RT-PCR method studied displayed high sensitivity and specificity in the detection of influenza virus in adult patients, when compared with the conventional techniques. With real-time RT-PCR, large numbers of samples can be rapidly tested and results provided the same day samples are received.Enfermedades Infecciosas y Microbiología Clínica 06/2009; 28(2):95-8. · 1.49 Impact Factor -
Article: Sensitivity evaluation of an immunochromatographic test for the rapid antigen detection of adenovirus.
Journal of Clinical Virology 08/2008; 42(3):291-2. · 3.97 Impact Factor -
Article: [Detection of oseltamivir resistance among human influenza A (H1N1) strains isolated in 2007-2008 flu season].
Medicina Clínica 07/2008; 131(2):63-4. · 1.38 Impact Factor -
Article: [Clinical and epidemiological characteristics of respiratory infections caused by human metapneumovirus in pediatric patients].
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ABSTRACT: Human Metapneumovirus (hMPV) was first identified in 2001 in respiratory samples from children and adults with acute respiratory tract infection. The aim of this prospective study was to determine the clinical and epidemiological characteristics of pediatric patients with an acute respiratory tract infection and exclusive isolation of hMPV in respiratory samples (December 2005-January 2007). All respiratory tract samples were submitted to rapid antigen detection against respiratory syncytial virus (RSV) and influenza A and B viruses. To isolate respiratory viruses, samples were inoculated in various cell lines using the shell vial culture assay. To isolate hMPV, the LLC-MK2 cell line was used. Only antigen-negative samples were studied for the presence of hMPV. Over the study period, 32 hMPV were isolated from different patients, accounting for 1.7% of all samples studied for this virus (only RSV-negative samples, 1,791) and 1.5% of all samples studied. Peak incidence was found between December and March of the two years studied. Of the 32 patients in whom hMPV was detected, 17 (53.2%) were female and 15 (46.8%) male. Mean age was 12.5 months (range, 1 month-4 years). The most frequent clinical symptoms were fever (90.6%), cough (87.5%), rhinorrhea and bronchiolitis (46.8%). Three patients (9.4%) were hospitalized. Respiratory infection caused by hMPV is considered an emergent disease in pediatric patients. The clinical manifestations are very similar to those of RSV infection; hence, only virological study can establish the definite etiological diagnosis.Enfermedades Infecciosas y Microbiología Clínica 03/2008; 26(2):72-6. · 1.49 Impact Factor -
Article: Comparison of different cell lines and incubation times in the isolation by the shell vial culture of human metapneumovirus from pediatric respiratory samples.
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ABSTRACT: We report a prospective study concerning the efficacy of LLC-MK2 (continuous monkey kidney cell), Hep-2, MDCK (Madin-Darby Canine Kidney), Vero and MRC-5 cell lines, by shell vial assay, and incubation time in the isolation of hMPV from pediatric respiratory samples. The overall sensitivity of the cell lines studied were: 100% for the LLC-MK2, 68.7% for the Hep-2, 28.1% for the Vero, 3.1% for the MDCK and 0% for the MRC-5. Only one strain (3.1%) showed growth in the four cell lines studied and 10 (31.2%) strains only grew in the LLC-MK2 cell line. The analysis of incubation times showed that only 14 strains (43.7%) were able to grow after 3 days of incubation, while all strains (100%) showed growth after 5 days. The use of shell vials with commercial LLC-MK2 cells could be a method for isolating hMPV from respiratory samples in the pediatric population.Journal of Clinical Virology 10/2007; 40(1):46-9. · 3.97 Impact Factor -
Article: [Neuraminidase inhibitors and their potential use in the avian influenza pandemic].
Medicina Clínica 01/2006; 125(20):780-3. · 1.38 Impact Factor -
Article: [Incidence of genital infections caused by herpes simplex virus type 1 (HSV-1) from 1995 to 2003].
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ABSTRACT: In recent years studies from various parts of the world have reported a significant increase in the number of genital infections caused by HSV-1, especially among women. We report a study of genital infections caused by HSV-1 in our geographic area from 1995 to 2003. Genital samples were inoculated in the Vero cell line and cultures were stained by monoclonal antibodies against HSV-1 and HSV-2. During the study period, 263 samples were positive for herpesviruses: 146 for HSV-1 (55.5%) and 117 for HSV-2 (44.5%). The 20 HSV-1 isolated in the genital tract represented 7.6% of all the herpesviruses isolated in this study. Of the 146 HSV-1 isolated, 20 (13.7%) were obtained in genital samples, 15 (75%) in women and 5 (25%) in men (p < 0.005). Four men (80%) were homosexual. No patient, male or female, was infected with the HIV and none worked as prostitutes. The percentage of HSV-1 genital infections increased in the last year of the study (33%), especially among women from the general population. The herpesvirus isolated in these samples should be characterized, since, in general, HSV-1 does not usually develop latency or recurrent infections in this anatomical area.Enfermedades Infecciosas y Microbiología Clínica 10/2005; 23(8):482-4. · 1.49 Impact Factor -
Article: [Avian influenza. A continual threat to human beings].
Medicina Clínica 04/2004; 122(9):339-41. · 1.38 Impact Factor -
Article: Comparison between indirect immunofluorescence assay and shell vial culture for detection of mumps virus from clinical samples.
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ABSTRACT: We report a prospective comparison of the efficacies of an indirect immunofluorescence assay (IFA) and shell vial culture (SVC) of throat swab and urine samples from patients with mumps. Throat swab samples were used for the IFA; the urine samples and throat swabs were inoculated into vials of Vero cells. We studied 62 patients by using 62 throat swabs and 50 urine samples (50 patients with both samples). Sixty (96.7%) throat samples were positive in the SVC, and 61 (98.3%) were positive in the IFA. For the 50 patients from whom both samples were available, the IFA was positive in 50 (100%) cases, the urine sample was positive in 49 (98%) cases, and the throat swab was positive in 48 (96%) cases (P > 0.05). This comparison of throat swabs and urine samples has shown that the two clinical samples are similar in efficacy.Journal of Clinical Microbiology 11/2003; 41(11):5186-7. · 4.15 Impact Factor -
Article: Evaluation of a new dot blot enzyme immunoassay (directigen flu A+B) for simultaneous and differential detection of influenza a and B virus antigens from respiratory samples.
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ABSTRACT: We report a prospective evaluation of a new dot blot enzyme immunoassay (EIA) method for the direct, rapid, qualitative, simultaneous, and differential detection of the influenza A (IA) and B (IB) virus antigen in different respiratory samples. The EIA method was compared with the shell vial culture system (MDCK cell line) used with the same samples. We studied 160 samples from 93 (58.1%) pediatric patients (hospital emergency room) and from 67 (41.9%) adult patients (sentinel network). Seventy-four(46.2%) samples were considered positive; of them, 46 (62.2%) were from pediatric patients and 28 (37.8%) were from an adult group (P < 0.05), with overall positive values of 49.9% and 41.7%, respectively. All 74 (100%) of the positive samples were isolated in cell culture versus the 68.9% that were detected as positive by the new EIA method (P < 0.05). Of the 41 samples positive for the IA virus, the EIA detected 34 (82.9%) positive samples; of the 33 samples positive for the IB virus, the EIA detected 17 (51.5%) positive samples (P < 0.05). No false-positive reaction was detected with the EIA method (specificity and positive predictive value of 100%). The overall results obtained in the comparison between the new EIA and the shell vial culture had a sensibility of 82.9% and predictive negative values of 92.4% for the IA virus and 51.5% and 84.3%, respectively, for the IB virus. This evaluation shows sensitivity and specificity percentages for the new EIA method that is acceptable for routine use in IA virus detection. The results obtained were worse for IB virus detection, but this new EIA method is actually the only one with the capacity to differentiate between the two influenza viruses.Journal of Clinical Microbiology 09/2002; 40(9):3515-7. · 4.15 Impact Factor -
Article: [Preliminary assessment of an immunochromatographic method (Directigen EZ-RSV) for antigenic detection of syncytial respiratory virus].
Enfermedades Infecciosas y Microbiología Clínica 23(7):455-6. · 1.49 Impact Factor -
Article: [Prevalence of genital infection due to Chlamydia trachomatis in the general population and in a group of prostitutes in Palma de Mallorca].
Enfermedades Infecciosas y Microbiología Clínica 22(7):439-41. · 1.49 Impact Factor -
Article: [Evaluation of a dot-blot enzyme immunoassay system for the detection of Influenza B/Hong Kong/330/01 antigen in children (2002-2003)].
Enfermedades Infecciosas y Microbiología Clínica 22(6):367. · 1.49 Impact Factor -
Article: [Factors affecting the virulence and pathogenicity of avian and human viral strains (influenza virus type A)].
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ABSTRACT: Most studies performed in avian viral strains seem to indicate that virulence is a polygenic phenomenon. However, hemagglutinin and neuraminidase and the genes codifying these substances (genes 4 and 6) play an essential role in viral pathogenesis. Avian strains can be classified as avirulent or virulent according to the ability of hemagglutinin to be activated by endoproteases of the respiratory tract only or by proteases from other tissues. This ability is based on the progressive development of mutations that lead to the substitution of the normal amino acids at the point of hemagglutinin hydrolysis by the other basic amino acids that determine the amplification of the spectrum of hydrolysis and activation. Neuraminidase participates in the acquisition of virulence through its capacity to bind to plasminogen and by increasing the concentration of activating proteases. Adaptation to the host, through recognition of the cell receptor, is another factor determining the virulence and interspecies transmission of avian strains. From an epidemiological point of view, viral strains should be subtyped and the activating capacity of hemagglutinin should be determined to identify their degree of virulence.Enfermedades Infecciosas y Microbiología Clínica 20(7):346-53. · 1.49 Impact Factor -
Article: Características clínicas y epidemiológicas de las infecciones respiratorias causadas por el metapneumovirus humano en pacientes pediátricos
Enfermedades infecciosas y microbiología clínica, ISSN 0213-005X, Vol. 26, Nº. 2, 2008, pags. 72-76.
Top co-authors
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Institutions
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2011
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Hospital Universitari Son Espases
Palma, Balearic Islands, Spain
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2005–2011
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Hospital Son Dureta
Palma, Balearic Islands, Spain
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