W.T. FELIPPE

Federal University of Santa Catarina, Nossa Senhora do Destêrro, Santa Catarina, Brazil

Are you W.T. FELIPPE?

Claim your profile

Publications (45)45.77 Total impact

  • [Show abstract] [Hide abstract]
    ABSTRACT: Introduction In individuals with periodontal disease, dental pulp status should be determined before a treatment plan is made. Pulse oximeters are promising diagnostic tools to evaluate pulp vascularization. This study used pulse oximetry to determine the level of oxygen saturation in dental pulp of intact permanent teeth with periodontal attachment loss (PAL) and gingival recession (GR) and to evaluate the correlation between periodontal disease and level of oxygen saturation in the pulp. Methods This study included 67 anterior teeth of 35 patients; all teeth showed intact crowns, PAL, a periodontal pocket (PP), and GR. The teeth underwent periodontal examination, cold and electric pulp testing, and pulse oximetry measurements. The Pearson correlation coefficient and a linear regression coefficient were calculated to evaluate the degree of correlation between periodontal disease markers (PAL, PP, and GR) and the level of oxygen saturation in dental pulp. These tests also evaluated possible associations between oxygen saturation and cold and electric pulp testing. Results PAL, PP, and GR had negative correlations with oxygen saturation in dental pulp. Conversely, no statistically significant association was found between oxygen saturation in dental pulp and the response to electric sensibility testing. Conclusions Oxygen saturation was lower in the pulp of permanent teeth with PAL, PP, and GR, indicating that periodontal disease correlates with the level of oxygen saturation in the pulp.
    Journal of Endodontics. 10/2014;
  • [Show abstract] [Hide abstract]
    ABSTRACT: This study evaluated the bond strength (BS) and the adhesive interface of four endodontic sealers to root canal dentine, before, and after immersion in phosphate buffered saline (PBS) to simulate an in vivo environment. Eighty roots were instrumented using ProTaper rotatory files, under irrigation with 17% EDTA and 1% NaOCl. Posteriorly were divided into four groups (n = 20) according to the sealer used: Endofill, AH Plus, Sealapex, and MTA Fillapex. Each group was divided into two subgroups (n = 10) and stored at 37°C immersed in water for 7 days and in PBS for 60 days. From each subgroup, 1 mm thick sections were obtained. One section of each region (coronal, middle, and apical) was submitted to the push-out test and failures were observed. Twelve sections of each subgroup (four from each region) were evaluated under SEM. Three-way ANOVA evaluation for BS showed significant differences between groups and regions (P < 0.0001), but not between subgroups (P > 0.05). AH Plus had significantly higher BS than the others sealers, regardless of the analyzed subgroup (Tukey's test, P < 0.5). The most common failures were adhesive to dentine and cohesive of the sealer. The SEM evaluation (Kruskal–Wallis, Mann–Whitney) showed homogeneous adhesive interface formed and sealer tags in all groups with significant statistical differences with AH Plus, regardless of PBS immersion. AH Plus was superior to the other sealers for both BS and quality of interface formation. Immersion in PBS did not interfere on BS or adhesive interface of the sealers tested. Microsc. Res. Tech., 2014. © 2014 Wiley Periodicals, Inc.
    Microscopy Research and Technique 09/2014; · 1.59 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Introduction Pulse oximetry is a noninvasive method for assessing vascular health based on oxygen saturation level. The method has recently also been used to assess dental pulp vitality, but a median oxygen saturation level suggestive of normal pulp physiology has not been determined. The objective of this study was to make a critical analysis of the published research to establish the median oxygen saturation for the diagnosis of normal dental pulps in maxillary anterior permanent teeth using pulse oximetry. Methods Studies reporting on the use of pulse oximeters to determine oxygen saturation in dental pulps were retrieved using the MEDLINE, Scientific Electronic Library Online, and Cochrane Central Register of Controlled Trials databases plus a manual search of relevant references cited by selected articles. Different combinations of the terms “oximetry,” “oximeter,” “pulp,” “dental,” and “dentistry” were used in the search. Statistical analysis was performed for each group of teeth (central incisors, lateral incisors, and canines) using R statistical software (US EPA ORD NHEERL, Corvallis, OR) and a random effects model (P < .0001) with an I2 of 99%. Results Of the 295 articles found, only 6 met the inclusion criteria (472 teeth). Of these, the number of articles included in each analysis (according to tooth group) was as follows: all 6 studies (288 teeth) for central incisors at a median oxygen saturation of 87.73%, 3 studies (90 teeth) for lateral incisors at a median oxygen saturation of 87.24%, and 4 studies (94 teeth) for canines at a median oxygen saturation of 87.26%. Conclusions The median oxygen saturation in normal dental pulps of permanent central incisors, lateral incisors, and canines was higher than 87%.
    Journal of Endodontics. 01/2014;
  • Source
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: AimTo evaluate connective tissue reactions to iRoot SP (Innovative Bioceramics, Vancouver, BC, Canada), mineral trioxide aggregate (MTA) Fillapex (FLPX) (Angelus Soluções Odontológicas, Londrina, Brazil), DiaRoot Bioaggregate (DiaDent Group International, Burnaby, BC, Canada) and white MTA (Angelus, Londrina, Brazil) in Wistar rats. MethodologyA total of 128 dentine tubes filled with the materials and 32 empty tubes (control) were implanted into 32 rats. After 7, 15, 30 and 90 days (n = 8 per period), the animals were euthanized, and the tissues were processed for histological evaluation using haematoxylin-eosin (H&E) and Von Kossa (VK) staining. Observations were made for cellular inflammatory components and the presence of multinucleated giant cells (MNGC), macrophages and tissue necrosis. Data were analysed by Fisher's exact and Kruskal–Wallis tests (P < 0.05). ResultsIn all experimental periods, MTA FLPX and iRoot SP scored higher than the other groups for the variable macrophages (P < 0.05). After 30- and 90-day experimental periods, MTA FLPX scored higher than the other groups for the variable MNGC (P < 0.05). After 90 days, the only group that exhibited samples with severe inflammatory response was MTA FLPX. VK positivity was observed in areas of necrosis in all groups, except in the control group. Conclusions The materials were considered biologically acceptable except MTA FLPX, which remained toxic to subcutaneous tissue even after 90 days.
    International Endodontic Journal 11/2013; · 2.05 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to evaluate the influence of exposure of the mineral trioxide aggregate (MTA) - with and without calcium chloride (CaCl2) - to phosphate-buffered saline (PBS) on the apical microleakage using a glucose leakage system. Sixty root segments were randomly divided into 4 experimental groups (n=15). After resecting the apical segments and enlarging the canals with Gates-Glidden drills, the apical cavities were filled with MTA with or without CaCl2 and the root canals were dressed with a moistened cotton pellet or PBS, as follows: 1) MTA/cotton pellet; 2) MTA/PBS; 3) MTA+10%CaCl2/cotton pellet; 4) MTA+10%CaCl2/PBS. All root segments were introduced in floral foams moistened with PBS. After 2 months, all root segments were prepared to evaluate the glucose leakage along the apical plugs. The amount of glucose leakage was measured following an enzymatic reaction and quantified by a spectrophotometer. Four roots were used as controls. The data were analyzed using Kruskal-Wallis and Mann-Whitney tests (p<0.05). There were no differences between groups 1 and 2 (p>0.05), and 3 and 4 (p>0.05). The addition of CaCl2 to the MTA significantly decreased its sealing ability (p<0.05). The interaction with PBS did not improve the MTA sealing ability. The addition of CaCl2 to the MTA negatively influenced the apical seal.
    Journal of applied oral science: revista FOB 08/2013; 21(4):341-5. · 0.39 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: To analyse the influence of exposure of mineral trioxide aggregate (MTA) with and without calcium chloride (CaCl2 ) to phosphate-buffered saline (PBS) on the push-out bond strength, over different experimental periods. One hundred and twenty dentine discs with standardized cavities were filled with MTA with and without 10% CaCl2 . The specimens were randomly divided as follows (n = 30): (G1) MTA in contact with a moistened cotton pellet, (G2) MTA immersed in PBS, (G3) MTA + CaCl2 in contact with a moistened cotton pellet and (G4) MTA + CaCl2 immersed in PBS. The samples were stored for 3, 28 and 60 days. The bond strengths were measured with the Instron Testing machine. Data were analysed using the three-way anova and Tukey test (P < 0.05). In general, the samples of MTA with and without CaCl2 , exposed to PBS, had higher bond strength values in all study periods (P < 0.05). Analysis of the influence of addition of CaCl2 to MTA (G1 × G3) evidenced significant differences in bond strength in the different periods (P < 0.05). The exposure of MTA to PBS positively influenced the push-out bond strength, whereas the addition of CaCl2 had a negative influence.
    International Endodontic Journal 07/2013; · 2.05 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: : The aim of this ex vivo study was to evaluate the effects of NaOCl and EDTA solutions, prior to root canal dressing with calcium hydroxide, on compressive strength of root dentin. Method: Forty-eight roots with single and straight canals were used. The endodontic treatment was conducted by the crown-down technique, and canals were irrigated with 2 ml of 0,9 % NaCl between each file or drill. Further, specimens were randomly divided into four groups (n = 12), according to the irrigating solution used: group 1 (control), 0.9% NaCl; group 2, 1% NaOCl; group 3, 17% EDTA; group 4, 1% NaOCl and 17% EDTA. The total time of irrigation in each group was 40 min, and every 10 min the tested solution was renewed. In group 4, the time was equally divided (alternating solutions every 10 min). Further, each root canal was dry and filled with calcium hydroxide paste. After X-rays to confirm the completion, each canal was sealed and stored at 37º C for 30 days. After this period, the roots were cut 1 mm below the cement-enamel junction and 6 mm apically from this cut, in order to obtain 6 mm-length cylinders. The compressive strength test was conducted in an Instron machine with a crosshead speed of 1mm/min. Result: The data recorded at fracture (MPa) were analyzed by ANOVA and Tukey test (α = 5%) and it was found that there was statistically significant difference between group 2, irrigated with NaOCl, and group 4, irrigated with EDTA and NaOCl. Group 2 had a mean compressive strength superior to that of Group 4. Conclusion: The root canal irrigation with 17% EDTA followed by 1% NaOCl promote a significant reduction of root dentin resistance observed after 30 days of calcium hydroxide dressing.
    IADR/AADR/CADR General Session and Exhibition 2013; 03/2013
  • [Show abstract] [Hide abstract]
    ABSTRACT: Many articles demonstrated that Passive Ultrassonic Irrigation (PUI) improves the cleaning and the smear layer removal. However no article has yet shown if the PUI influences on hydroxil ions (OH-) diffusion on dentin. Objective: The aim of this study was to evaluate the PUI effect on OH- diffusion arising from calcium hydroxide paste among root dentin. Methods: 50 humans teeth were endodontically prepared. The cement layer was removed and the root surface was washed with EDTA and NaOCl. The cementoenamel junction and the apical portion of each root was covered. 4 different protocols for final irrigation were employed (n=10): G1 - final irrigation with EDTA + NaOCl, G2 - final irrigation with EDTA + PUI + NaOCl, G3 – final irrigation with EDTA + NaOCl + PUI and G4 – final irrigation with EDTA + NaOCl both with PUI activation. 5 teeth received only distilled water as final solution (G5) and other five were irrigated with distilled water + PUI (G6) working was control groups. The root canal was then dried and filled with CH. The specimens were individually kept in plastic vials containing distilled water (10mL) with a known pH. After 7, 15, and 21 days the pH from the specimens distilled water was verified. This values were recorded and compared by using ANOVA and Tukey’s test was used to individuals comparisons (α=0.05). Results: No statistically significant difference was observed between the groups (p=0.1224). But it was observed among the different periods (p<0.0001). In the 15 and 21 days period was more OH- diffusion when compared to the 7 days analysis. Conclusion: PUI did not influence on OH- diffusion among radicular dentin. When CH is employed as an intracanal medication it is necessary at least 15 days for the paste to promote a proper alcalinization of the dentin.
    IADR/AADR/CADR General Session and Exhibition 2013; 03/2013
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: To evaluate ex vivo the effects of immersion in phosphate buffer solution (PBS) in bond strength (BS) and adhesive interface formation between endodontic sealers and root canal dentin. Methods: Eighty 16-mm long roots had their canals submitted to biomechanical preparation. After the final irrigation with EDTA and NaOCl, the canals were randomly divided into four groups (n = 20) according to the sealer used in obturation: Endofill (G1), AH Plus (G2), Sealapex (G3) and MTA Fillapex (G4). Half of the specimens of each group was tested after 07 days of immersion in water (Period A) and the other half after 60 days in PBS (period B). Two cross-sections of 1 mm thickness for each region (coronal, middle and apical) of the root were obtained. One slice per region was used to push-out test and the other one was prepared for interface analysis in scanning electron microscopy (SEM). Results: Three-way analysis of variance (α = 0.05) indicated statistically significant difference among the groups and the regions (p <0.0001), but not among periods. AH Plus (G1) had higher BS than the other sealers, regardless of the period analyzed (p <0.5). Adhesive failures were more commonly observed in the G1 Group (Endofill), and cohesive failures in the other groups. SEM analysis showed In both periods A and B, homogeneous adhesive interface and the presence of many tags when the AH Plus sealer was used. After 60 days of immersion in PBS it was observed greater depth of sealer penetration into dentinal tubules,only when Sealapex and MTA Fillapex were used. Conclusion: The immersion in PBS did not increase the bond strength to dentin of the tested sealers, although after this period the SEM analysis showed more homogeneous adhesive interface.
    06/2012
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objectives: To evaluate the influence of two intensities of pressure (30KPa and 60KPa) in bacterial penetration in filled root canals, and to correlate the depth of penetration with the time of exposure to the oral environment (45 and 120 days). Methods: One hundred-twenty-two root canals of dogs’ teeth were instrumented with ProTaper files and filled by single cone technique with gutta-percha and AH Plus sealer. After setting, root canal fillings were subjected to different pressures for 8 minutes according to groups: A-were not under pressure (n=34); B-30KPa (n=34); C-60KPa (n=34). Then, all root canal fillings were exposed to the oral environment in accordance with sub-groups (45 and 120 days). All sub-groups had positive (n=10) and negative controls (n=10). The animals were killed and jaws were removed and prepared for histobacteriological analysis. Histological sections were stained with Brown-Brenn to detect the presence of bacteria. Results: Bacterial infiltration was higher in cervical 4mm. The Kruskal-Wallis test did not shown significant difference in bacterial penetration among groups A, B and C in both periods: 45 days (p=0.903) and 120 days (p=0.211). Comparing the exposure times (45 and 120 days), there was no significant difference in bacterial penetration (p=0.608), although greater infiltration was detected in 120 days. Conclusions: The pressures of 30KPa and 60KPa did not induce alterations in root canal fillings to the point of influencing bacterial penetration in the different time periods. These pressures can be indicated to use in sealability assessment methodologies (ex vivo and in vitro) to facilitate the infiltration of fluid markers such as dyes, water and glucose.
    IADR General Session 2012; 06/2012
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: The aim of this study was to evaluate the coronal leakage in root canals filled with gutta-percha and AH Plus and MTA FILLAPEX. Method: After accessing, measuring and cleaning of the 36 single-rooted premolars root canals, the instrumentation was performed with the ProTaper system, until to instrument F4 under irrigation with 1%. sodium hypochlorite solution Thirty teeth were randomly divided in two experimental groups (n = 15) according to the sealer used: G1: MTA FILLAPEX and G2: AH Plus. After obturation, with ProTaper F4 cones, the teeth were kept at 37 °C for 07 days. Six teeth were used as positive control group (n = 3) and negative control group (n = 3). The infiltration test was realized with dye ink under a pressure of 60 kPa for 8 min. After diaphanization, the dye penetration was evaluated by root thirds. Result: There was infiltration of 8 and 7 samples of G1 and G2, respectively. In any sample of the G2, the dye reached the apical third, being restricted to the cervical and middle thirds. In four samples of G1, the dye was visualized in the apical third. Evaluating the total infiltration, the χ² test and Fisher's exact test revealed no significant difference between groups 1 and 2. When the dye penetration was evaluated by thirds, the proportions test showed that, for the apical third, G12 were significantly different (p = 0.0317). Conclusion: It was concluded that the two sealers allowed, similarly, the dye leakage in the cervical and middle thirds of the root. When considering infiltration to the apical third, the MTA FILLAPEX showed a lower quality of sealing.
    IADR General Session 2012; 06/2012
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: Mineral Trioxide Aggregate (MTA) has as radiopacifier bismuth oxide (Bi2O3), which has received criticism for not taking part of the material setting and for make it more porous. Portland cement has similar composition and lower price as compared to MTA and because of that, Portland cement has replaced MTA in many researches. This study aimed to evaluate the solubility and the disintegration of Portland cement with different concentrations of Bi2O3 (0, 10, 15, 20 e 30%). Method: Twelve teflon rings with 4 mm thickness and a 12 mm diameter were filled with cement. After setting, the samples were weighed (initial hidrated weight = PH0) and after 24 h in a dissecator, the samples were again weighed (initial dehydrated weight = PD0). Then the samples were divided and immersed in deionized water for 7 and 30 days. After those periods, the same procedures were performed and new weights were obtained (PH7d, PD7d, PH30d e PD30d). The solubility and disintegration of the cements was measured by the loss of mass identified by the weight of each sample, expressed as percentage of weight lost when compared to the original weight. For statistical analysis it was employed the two-way ANOVA test, in each period, and the Tukey HSD to indicate the significant differences (α=5%). It was not observed disintegration of the cements. Result: There was a difference between the groups in the 7 days analysis (hidrated and dehydrated) and between the periods for pure Portland cement when weighed dehydrated (p<0,05). Conclusion: Although there was statistical difference in the solubility of the groups at 7 days, all showed the same behavior after 30 days of storage in water.
    IADR General Session 2012; 06/2012
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objectives: To investigate, in an ex vivo apexification model, if the interaction of the MTA-dentin system with phosphate-buffered saline (PBS) and if the addition of calcium chloride (CaCl2) 10% to MTA influence the apical seal. Method: Sixty root segments were randomly divided into two groups (n=30) according to the cement used to form the apical plug: G1, MTA and G2, MTA + 10% CaCl2. Root segments were introduced in floral foams moistened with PBS and subdivided: G1A and G2A, a wet cotton pellet was placed in the cervical region for 24h and, after, replaced by a dry one; G1B and G2B, the remaining canal space was filled with PBS as an intracanal dressing. All access openings were filled and, after 2 months, prepared in a double chamber apparatus to evaluate the glucose leakage along the apical plugs. The amount of glucose leakage was quantified by a spectrophotometer. Data were analyzed by using Mann-Whitney test. Significance was set at α=5%. Results: There were no differences between groups 1A and 1B (p=0,258), and 2A and 2B (p=0,287); however, root segments dressed with PBS showed smaller number of samples with traces of the solution and lower glucose concentration. The addition of CaCl2 to the MTA significantly decreased its sealing ability (G1A x G2A, p=0,003) (G1B x G2B, p=0,019), mainly in samples from G2A. Conclusions: Despite there were no differences between groups 1A and 1B, and 2A and 2B, the interaction of the MTA-dentin system with PBS positively influenced the apical seal. The addition of CaCl2 to the MTA negatively influenced the apical seal.
    IADR General Session 2012; 06/2012
  • [Show abstract] [Hide abstract]
    ABSTRACT: Post space preparation of endodontically treated teeth should be carefully done, preserving a great dentin thickness, and avoiding fractures and perforations. Objective: As periapical radiographs are not an appropriated method to measure dentin thickness, the goal of this study was to verify the accuracy of Cone Beam Computed Tomography (CBCT) in measuring the thickness of radicular dentin walls. Methods: Ten premolars were embedded in polystyrene acrylic resin and sections were made in two predetermined marks. Photographic images of the sections were taken and measured in Image J program, creating an anatomic thickness of the four walls of root dentin (buccal, lingual, mesial, distal). The tomographic images of the slices were taken with the iCAT system and measured with ImageJ and i-CAT view software. Results: The results were recorded and a variance analysis was used to compare the measures (ANOVA three-way), with the significance level set at 5%. There was no significant difference when comparing methods of measuring the root dentin thickness (p<0.05). Conclusion: CBCT proved to be a reliable method to measure the radicular dentin thickness.
    IADR General Session 2012; 06/2012
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: This study aimed at analyzing the tissue response stimulated by the endodontic cements Mta Fillapex and Diaroot in subcutaneous tissue of rats. Method: Seventy two human dentin tubes were divided in 3 groups, filled with the experimental cements or kept empty for control group. The tubes were implanted in the dorsal area of 24 rats. After 7, 30 and 90 days, the animals were euthanized and the tissue in contact with the tube was removed, processed and stained. An evaluation of the tissue reactions was performed according to these events: definition and composition of the connective tissue capsule, blood vessel neo-formation, and presence of multinucleated giant cells, macrophages and severity of inflammation. Result: Mta Fillapex stimulated a moderate inflammatory reaction after 7 and 30 days in all specimens, and the connective tissue capsule was composed by granulation tissue. After 90 days, a moderate infiltrate was observed in 4 of the 7 samples, mild infiltrate in 2 samples and severe in 1 sample. On 7 days, Diaroot stimulated a mild infiltrate of inflammatory cells in 5 samples and moderate infiltrate in 2 samples with the tissue capsule constituted by granulation tissue in almost all samples. After 30 days, Diaroot showed a mild inflammatory infiltrate in 4 of the 8 samples, absent infiltrate in 1 and moderate in 2 samples, with connective tissue capsule constituted by granulation tissue. After 90 days, Diaroot elicited a mild inflammatory reaction in all samples with a capsule constituted by fibrous tissue. The control group stimulated a similar reaction when compared with the two cements after 7 days, and showed a reduction of the inflammatory reaction after 30 and 90 days. Conclusion: Diaroot was considered more biocompatible and showed better results when compared to Mta Fillapex. The control group and Diaroot presented similar results.
    06/2012
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: To evaluate the influence of apical patency on the periapical healing process in pulpless dogs’ teeth after root canal filling with AH Plus sealer. Methods: Seventy-eight root canals of dogs’ teeth were used. After pulp extirpation, the cemental canal was penetrated and widened up to a size 20 K-file. After, the canals were exposed to the oral cavity for 3 months for contamination. Root canals were randomly divided into 6 groups (n=13), and prepared up to size 50 K-file, maintaining (PAT) or not (NPAT) the apical patency with a size15 K-file, and dressing or not the root canals with calcium hydroxide paste (CH) for 30 or 7 days, as described: Group 1: PAT+CH for 30 days; Group 2: NPAT+CH for 30 days; Group 3: PAT+CH for 7 days; Group 4: NPAT+CH for 7 days; Group 5: PAT+ immediate root canal filling; and Group 6: NPAT+ immediate root canal filling. After 6 months, the jaws were prepared for histological analysis, and sections evaluated for quality of the apical foramen closure, presence of active resorption areas, and intensity of inflammatory reaction. Data were analyzed by Kruskal-Wallis test (p <0.05). Result: Despite of time and use of HC paste, apical patency had no effect in any of the parameters evaluated. Conclusion: It was concluded that maintenance of the apical patency does not influence the periapical healing of previously contaminated dogs' teeth.
    IADR General Session 2012; 06/2012
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objectives: The aim of this research was to evaluate the diffusion of hydroxyl ions from calcium hydroxide paste (CHP) placed in root canal before and after the removal of the filling material and re-instrumentation. Methods: Sixty root canals were shaped by step-back technique, 1 mm short of the apical foramen under irrigation with sodium hypochlorite 1% (SH). At the end of instrumentation, the root canals were irrigated with EDTA17% and SH for smear layer removal. The cementum layer was removed, the root surface was washed with EDTA, and the apical 3 mm of each root were covered with a fast-setting adhesive. The canals were filled with CHP, and after sealing the access openings, the teeth were washed and placed in individual vials containing 10 mL of distilled water, which had its pH measured after 7, 14, 21 and 28 days (pH1). After removal of CHP, the root canals were divided into five equal groups and filled with Resilon / Real Seal or gutta-percha and Endofill, Sealapex, AH Plus or MTA Fillapex sealers. After storage for 7 days at 37° C in 100% relative humidity, the root canals were re-treated. After the final irrigation with EDTA and HS, the CHP was again inserted into the canals, the teeth sealed, washed and placed in new vials containing 10 mL of distilled water. Again the pH of water was measured at 7, 14, 21 and 28 days (pH2). The initial and final pH readings (pH1 and pH2) were compared by t test (p < 0.05). Results: The pH readings obtained after retreatment (pH2) were significantly higher than those obtained before root canal filling (pH1). Conclusions: Despite of the sealer employed, it is possible to re-establish dentin permeability to ionic diffusion after retreatment.
    IADR General Session 2012; 06/2012
  • [Show abstract] [Hide abstract]
    ABSTRACT: Objective: The aim of this study was to analyze the interaction of the new cements MTA Fillapex, Diaroot Bioaggregate and iRoot SP with dentin in vivo. Method: One hundred and sixty human dentin tubes were randomly divided in 4 experimental groups. After filling with the materials tubes were implanted subcutaneously in 32 rats at four sites equidistant from the dorsal area. Empty tubes were used as control and implanted in a fifth site. After 7, 15, 30 and 90 days the animals were euthanized and the dentin tubes retrieved for scanning electron microscope (SEM) analysis. Result: In 7 and 30 days, the mineral deposition in the material-dentin interface was detected in all tubes filled with MTA Fillapex; and in 15 and 90 days it was detected in 7 of the 8 samples. Despite of the period, the mineral deposition was not detected in any of the control samples, neither in those filled with iRoot SP, Diaroot Bioaggregate and MTA Branco. Conclusion: It was concluded that MTA FILLAPEX was the only cement which interacted with dentin and promoted the in vivo biomineralization, and that this process was not affected by the experimental period.
    IADR General Session 2012; 06/2012
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Mineral trioxide aggregate (MTA) and Portland cement have been shown to be bioactive because of their ability to produce biologically compatible carbonated apatite. This study analyzed the interaction of MTA and white Portland cement with dentin in vivo. Seventy-two human dentin tubes were filled with MTA Branco, MTA BIO, and white Portland cement + 20% bismuth oxide (PC1) or PC1 + 10% of calcium chloride (PC2) and implanted subcutaneously in 18 rats at 4 sites from the dorsal area. Empty dentin tubes, implanted in rats of a pilot study, were used as control. After 30, 60, and 90 days, the animals were killed, and the dentin tubes were retrieved for scanning electron microscope analysis. In the periods of 30 and 60 days, the mineral deposition in the material-dentin interface (interfacial layer) and in the interior of dentinal tubules was detected in more tubes filled with MTA Branco and MTA BIO than in tubes filled with PC1 and PC2. After 90 days, the interfacial layer and intratubular mineralization were detected in all tubes except for 3 and 1 of the tubes filled with PC2, respectively. It was concluded that all the cements tested were bioactive. The cements released some of their components in the tissue capable of stimulating mineral deposition in the cement-dentin interface and in the interior of the dentinal tubules. MTA BIO and MTA Branco were more effective in promoting the biomineralization process than Portland cements, mainly after 30 and 60 days.
    Journal of endodontics 03/2012; 38(3):324-9. · 2.95 Impact Factor