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ABSTRACT: Diphysidione (1), a new p-hydroxyphenyl anhydride, and vitexin (2) were isolated from the leaves of Diphysa carthagenensis. The structure of the new metabolite was characterised as 4-(4-hydroxy-phenyl)-3H-pyran-2,6-dione using 1D and 2D NMR spectroscopy.
Natural product research 03/2012; · 1.01 Impact Factor
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ABSTRACT: To investigate the potential of plants used in Mayan traditional medicine to treat cancer-like symptoms using the Mayan ethnobotany literature, and evaluate their organic extracts for in vitro cytotoxic activity on cancer cell lines.
The selection of the plants studied in this investigation was based on the Mayan ethnobotanical information provided by different literature sources. Extracts were obtained by maceration with methanol for 72 h of each plant part used and evaporated in vacuo to give the corresponding dried extract. Each methanol extract was tested for its cytotoxicity using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay performed in 96-well tissue plates on seven cancer cell lines, lung carcinoma (A549), cervix adenocarcinoma (HeLa), laryngeal carcinoma (Hep-2), nasopharynx carcinoma (KB), breast adenocarcinoma (MCF-7), prostate adenocarcinoma (PC-3), and cervix squamous carcinoma cells (SiHa), as well as normal human embryonic kidney cell line (HEK-293). Cell proliferation/viability was spectrophotometrically assessed at 540 nm after addition of MTT.
51 plants were found in the literature to be used for the treatment of symptoms suggestive of cancer, 21 were chosen to evaluate the cytotoxic activity. Aeschynomene fascicularis root bark extract showed a pronounced cytotoxic activity on Hela and KB cell lines and Bonellia macrocarpa stem and root bark extracts showed similar prominent activities on KB cells.
21 plants were selected according to their use in the treatment of cancer-like symptoms recorded in the ethnobotanical literature. Plant extracts prepared from Aeschynomene fascicularis root bark and Bonellia macrocarpa stem and root bark have been selected for extensive studies leading to the isolation of the active constituents.
Journal of ethnopharmacology 06/2011; 135(3):719-24. · 2.32 Impact Factor
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ABSTRACT: The in vitro leishmanicidal effect of (3S)-16,17-didehydrofalcarinol (1) isolated from Tridax procumbens whole plant against Leishmania mexicana, the causative agent of cutaneous leishmaniasis (chiclero's ulcer) in the New World, was investigated. This oxylipin showed significant in vitro activity against promastigotes and intracellular amastigotes of L. mexicana. Its inhibitory effect on amastigotes was not due to activation of NO in recombinant gamma-interferon-stimulated macrophages, since the production of NO was decreased in presence of the oxylipin. This is the first report on the leishmanicidal activity against the intracellular stage (amastigote) of the oxylipin (3S)-16,17-didehydrofalcarinol.
Phytotherapy Research 12/2009; 24(7):1004-8. · 2.09 Impact Factor
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ABSTRACT: Tridax procumbens is an active herb against leishmaniasis.
Leishmaniasis is a group of diseases caused by Leishmania protozoa. We investigated the antileishmanial activity of Tridax procumbens extracts and a pure compound against promastigotes of Leishmania mexicana, the causative agent of cutaneous leishmaniasis in the New World.
Extracts and (3S)-16,17-didehydrofalcarinol (1) were obtained by chromatographic methods from Tridax procumbens, and the latter identified by spectroscopic analysis. The effect of these extracts and 1 on the growth inhibition of promastigotes of Leishmania mexicana was evaluated. In order to test the safety of extracts and 1, mammalian cells were treated with them, and cell viability was assessed using trypan blue and MTT.
We demonstrated that extracts of Tridax procumbens and 1 showed a pronounced activity against Leishmania mexicana. The methanol extract inhibited promastigotes growth of Leishmania mexicana with a 50% inhibitory concentration (IC(50)) of 3 microg/ml, while oxylipin 1 exhibited the highest inhibition at IC(50)=0.478 microg/ml.
In this study we report the biological activity of extracts and (3S)-16,17-didehydrofalcarinol (1), obtained from Tridax procumbens, on the promastigote form of Leishmania mexicana, with no effect upon mammalian cells.
Journal of ethnopharmacology 05/2009; 122(3):463-7. · 2.32 Impact Factor
